Structural and molecular genetical analyses of factors related to bacterial chromosome condensation

• Project/Area Number: 21570125
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: KAMADA Katsuhiko 独立行政法人理化学研究所, 平野染色体ダイナミクス研究室, 専任研究員 (70360526)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 染色体凝縮

Research Abstract

I have identified functional regions of regulator subunits(ScpA and ScpB) of the bacterial condensin, and investigated their biochemical properties. In addition, using various mutants of the subunits, I examined the ATPase activity of the SMC subunit. As a result, some peptide regions of ScpA, through binding of the C-terminal domain of ScpB, control the ATPase activity of the SMC subunit. This result suggests that internal conformational changes of the regulatory subcomplex organize dimerization of the SMC subunit in the bacterial condensin.

Research Products

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  • URL: http://www.riken.jp/chromdyna/