| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
Expression and purification of human methionine synthase, methionine synthase reductase(MSR), and methylenetetrahydrofolate reductase(MTHFR) have been achieved. A common polymorphism in MSR gene, 66A> G, did not influence on protein function. Also, effects of gene mutations found in severe MTHFR-deficient patients were examined, i. e. properties of these mutant proteins were biochemically characterized. In addition, protein structural analysis using Thermus thermophilus HB8 MTHFR as model rationally explained how the gene mutations affected protein properties.
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