| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Research Abstract |
Dnmt3a creates DNA methylation patterns. Dnmt3a2, an isoform of Dnmt3a, lacks amino-terminal 219 amino acids of Dnmt3a and is highly expressed in germ cells and early embryo. We found that the amino-terminal 219 amino acids shows DNA binding activity and contributes for its localization and for DNA methylation activity. On the other hands, DNA methylation pattern once created is maintained by Dnmt1. We solved crystal structure of mouse Dnmt1(291-1602 amino acids) at 1. 3 A resolution. Notably, in the absence of DNA, the amino-terminal domain responsible for targeting Dnmt1 to replication foci is inserted into the DNA-binding pocket, indicating that this domain must be removed during methylation reaction.
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