| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
We here show that BIG2 plays an important role in a formation of Glut4 vesicles from endosomal compartment in both basal and insulin-stimulated adipocytes. In 3T3-L1 adipocytes, knockdown of BIG2 by RNA interference leaded to insufficient insulin-stimulated Glut4 translocation to the plasma membrane. The basal distribution of Glut4 was analyzed by subcellular fractionation. It was shown that the formation of Glut4-vesicles in the BIG2-knockdown cells was strongly repressed. Here, we used an in vitro reconstitution of Glut4-vesicles in order to demonstrate that these Glut4-vesicles are formed from endosomal membranes in a BIG2-dependent manner. Reducing endogenous BIG2 inhibited in vitro formation of Glut4 vesicles from the endosomal compartment prepared from not only unstimulated but insulin-stimulated adipocytes.
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