| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Research Abstract |
Knockdown of diacylglycerol kinase_η(DGK_η) using siRNA inhibited cell proliferation of the HeLa cells. Moreover, DGK_ηactivateed the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by epidermal growth factor(EGF). DGK_ηregulated recruitment of B-Raf and C-Raf from cytosol to membranes and their heterodimerization in EGF-stimulated HeLa cells. DGK_ηalso activated C-Raf but not B-Raf in an EGF-dependent manner. These results suggest that DGK_ηacts as a novel critical regulatory component of the ERK signaling pathway. On the other hand, it is suggested that DGKα positively regulates tumor necrosis factor_α-dependent nuclear factor-_κB activation via the protein kinase Cζ-mediated Ser311 phosphorylation of p65 subunit in AKI melanoma cells.
|
