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Regulation of CD44-hyaluronan interaction by SHAP and its role in inflammation

Research Project

Project/Area Number 21570147
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionAichi Medical University

Principal Investigator

ZHUO Lisheng  愛知医科大学, 医学部, 准教授 (00399031)

Co-Investigator(Renkei-kenkyūsha) KIMATA Koji  愛知医科大学, 名誉教授 (10022641)
BABA Kenji  愛知医科大学, 医学部, 教授 (80211499)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords糖鎖生物学 / SHAP / ヒアルロン酸 / CD44 / 炎症 / 細胞接着
Research Abstract

CD44and its ligand hyaluronan emerge as important adhesion molecules for leucocyte infiltration in inflammatory response. We have found that the serum protein SHAP binds covalently to hyaluronan and enhances the adhesion of CD44-positive cells to hyalurona. In this project, we reveal that SHAP plays significant roles in in vivo inflammatory responses by using SHAP-deficient mice. Depending on the affected organ and disease model, the role of SHAP varies, either being inflammatory(such as in acute liver injury model) or being anti-inflammatory(such as in airway hyper-responsiveness model and intracerebral hemorrhage model).

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (50 results)

All 2012 2011 2010 2009

All Journal Article (14 results) (of which Peer Reviewed: 13 results) Presentation (34 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Inhibition of hyaluronan synthesis in breast cancer cells by4-Methylumbelliferone suppresses tumorigenicity in vitro and metastatic lesions of bone in vivo2012

    • Author(s)
      Urakawa H, Nishida Y, Wasa J, Arai E, Zhuo L, Kimata K, Kozawa E, Futamura N, Ishiguro N
    • Journal Title

      Int J Cancer

      Volume: 130(2) Issue: 2 Pages: 454-66

    • DOI

      10.1002/ijc.26014

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Clinicopathological role of serum-derived hyaluronan-associated protein(SHAP)-hyaluronan complex in endometrial cancer2011

    • Author(s)
      Yabushita H, Iwasaki K, Kanyama K, Obayashi Y, Zhuo L, Itano N, Kimata K, Wakatsuki A
    • Journal Title

      Obstetrics and Gynecology International

      Volume: 2011 Pages: 739150-739150

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of hyaluronan retention by4-methylumbelliferone suppresses osteosarcoma cells in vitro and lung metastasis in vivo2011

    • Author(s)
      Arai E, Nishida Y, Wasa J, Urakawa H, Zhuo L, Kimata K, Kozawa E, Futamura N, Ishiguro N
    • Journal Title

      Br J Cancer

      Volume: 105(12) Issue: 12 Pages: 1839-49

    • DOI

      10.1038/bjc.2011.459

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Clinicopathological Role of Serum-Derived Hyaluronan-Associated Protein (SHAP)-Hyaluronan Complex in Endometrial Cancer2011

    • Author(s)
      Yabushita H, Iwasaki K, Kanyama K, Obayashi Y, Zhuo L, Itano N, Kimata K, Wakatsuki A
    • Journal Title

      Obstet Gynecol Int

      Volume: 2011 Pages: 739150-739150

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Serum level of SHAP as a disease marker : in comparison with hyaluronan2010

    • Author(s)
      Zhuo L, Kimata K
    • Journal Title

      Trends Glycosci Glyc

      Volume: 22(124) Pages: 80-88

    • NAID

      10027508196

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deficiency of the SHAP-hyaluronan complex enhances airway hyperresponsiveness in a murine model of asthma2010

    • Author(s)
      Zhu L, Zhuo L, Kimata K, Yamaguchi E, Watanabe H, Aronica M, Hascall VC, Baba K
    • Journal Title

      Int Arch Allergy Imm

      Volume: 153(3) Pages: 223-233

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of hyaluronan synthase, hyaluronan and serum-derived hyaluronan-associated protein(SHAP)-hyaluronan complex in endometrial cancer2010

    • Author(s)
      Kanyama K, Yabushita H, Obayashi Y, Noguchi M, Zhuo L, Itano N, Kimata K, Wakatsuki A
    • Journal Title

      J Aichi Med Univ Assoc

      Volume: 38 Pages: 29-42

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of hyaluronan synthase, hyaluronan and serum-derived hyaluronan-associated protein (SHAP)-hyaluronan complex in endometrial cancer.2010

    • Author(s)
      Kanyama K, Yabushita H, Obayashi Y, Noguchi M, Zhuo L, Itano N, Kimata K, Wakatsuki A
    • Journal Title

      J Aichi Med Univ Assoc

      Volume: 38巻 Pages: 29-42

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deficiency of the SHAP-hyaluronan complex enhances airway hyperresponsiveness in a murine model of asthma.2010

    • Author(s)
      Zhu L, Zhuo L, Kimata K, Yamaguchi E, Watanabe H, Aronica M, Hascall VC, Baba K
    • Journal Title

      Int Arch Allergy Imm

      Volume: 153 Pages: 223-233

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hyaluronan mediates ozone-induced airway hyperresponsiveness in mice2009

    • Author(s)
      Garantziotis S, Li Z, Potts EN, Kimata K, Zhuo L, Morgan DL, Savani RC, Noble PW, Foster WM, Schwartz DA, Hollingsworth JW
    • Journal Title

      J Biol Chem

      Volume: 284(17) Pages: 11309-17

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inter-alpha-trypsin inhibitor promotes bronchial epithelial repair after injury through vitronectin binding2009

    • Author(s)
      Adair JE, Stober VP, Sobhany M, Zhuo L, Roberts JD, Negishi M, Kimata K, Garantziotis S
    • Journal Title

      J Biol Chem

      Volume: 284(25) Pages: 16922-30

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inter-alpha-trypsin inhibitor promotes bronchial epithelial repair after injury through vitronectin binding2009

    • Author(s)
      Adair JE, Stober VP, Sobhany M, Zhuo L, et al
    • Journal Title

      J Biol Chem 284(25)

      Pages: 16922-30

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hyaluronan mediates ozone-induced airway hyperresponsiveness in mice2009

    • Author(s)
      Garantziotis S, Li Z, Potts EN, Kimata K, Zhuo L, et al
    • Journal Title

      J Biol Chem 284(17)

      Pages: 11309-17

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2009

    • Author(s)
      卓麗聖, 木全弘治(田畑泰彦編集)
    • Journal Title

      ますます重要になる細胞周辺環境の科学技術-細胞の生存、増殖、機能のコントロールから再生医療まで(株式会社メディカルドゥ)

      Pages: 195-200

    • Related Report
      2009 Annual Research Report
  • [Presentation] Inter-a-Trypsin Inhibitor Ameliorates Endothelial Injury In Sepsis2011

    • Author(s)
      Stober V, Lim YP, Zhuo L, Peddada S, Kimata K, Garantziotis S
    • Organizer
      American Thoracic Society conference
    • Place of Presentation
      Denver, Colorado, USA
    • Year and Date
      2011-05-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] Inter-a-Trypsin Inhibitor Ameliorates Endothelial Injury In Sepsis2011

    • Author(s)
      Stober V, Lim YP, Zhuo L, Peddada S, Kimata K, Garantziotis S
    • Organizer
      American Thoracic Society conference(ATS)
    • Place of Presentation
      Denver, Colorado, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] Localization of hyaluronan in airways of patients with cough variant asthma2010

    • Author(s)
      Nishimura M, Baba K, Yamaguchi E, Zhuo L, Kimata K
    • Organizer
      Annual Congress of European Respiratory Society(ERS)
    • Place of Presentation
      Barcelona, Spain
    • Related Report
      2011 Final Research Report
  • [Presentation] Mechanism of the protective role of the SHAP-hyaluronan complex in the murine model of airway hyperresponsiveness2010

    • Author(s)
      Zhuo L, Zhu L, Zhu L, Baba K, Yamaguchi E, Kimata K
    • Organizer
      The 25th International Carbohydrate Symposium
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Characterization of influence of glycans on CD44variant form(CD44v8-10) for cell adhesion mediated by E-selectin2010

    • Author(s)
      Kanamori A, Zhuo L, Kimata K, Kannagi R
    • Organizer
      The 25th International Carbohydrate Symposium
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Evidence for the presence of a novel SHAP transferase2010

    • Author(s)
      Zhuo L, Nakamura T, Zhu L, Yang Q, Hamamaoto T, Kimata K
    • Organizer
      HA2010
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Inhibition of hyaluronan synthesis in breast cancer cells suppresses tumorigenecity in vitro and metastatic lesion of bone in vivo2010

    • Author(s)
      Urakawa H, Nishida Y, Wasa J, Arai E, Zhuo L, Kimata K, Kozawa E, Futamura N, Ishiguro N
    • Organizer
      HA2010
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Hyaluronan plays significant roles not only in maintenance of musculoskeletal systems but also in tumorigenecity of musculoskeletal malignant tumors2010

    • Author(s)
      Nishida Y, Wasa J, Urakawa H, Arai E, Shinomura T, Zhuo L, Kimata K, Knudson W, Knudson C, Ishiguro N
    • Organizer
      HA2010
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] 咳喘息の病態における気道内ヒアルロン酸(HA)の意義について2010

    • Author(s)
      西村眞樹, 馬場研二, 河合聖子, 田中博之, 横江徳仁, 高橋大輔, 八木健郎, 久保昭仁, 山口悦郎, 卓麗聖
    • Organizer
      第22回日本アレルギー学会春季臨床大会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Characterization of influence of glycans on CD44 variant form (CD44v8-10) for cell adhesion mediated by E-selectin2010

    • Author(s)
      Kanamori A, Zhuo L, Kimata K, Kannagi R
    • Organizer
      The 25^<th> International Carbohydrate Symposium
    • Place of Presentation
      幕張メッセ(千葉)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Mechanism of the protective role of the SHAP-hyaluronan complex in the murine model of airway hyperresponsiveness2010

    • Author(s)
      Zhuo L, Zhu L, Zhu L, Baba K, Yamaguchi E, Kimata K
    • Organizer
      The 25^<th> International Carbohydrate Symposium
    • Place of Presentation
      幕張メッセ(千葉)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Evidence for the presence of a novel SHAP transferase2010

    • Author(s)
      Zhuo L, Nakamura T, Zhu L, Yang Q, Hamamaoto T, Kimata K
    • Organizer
      HA2010
    • Place of Presentation
      ブライトンホテル京都
    • Related Report
      2010 Annual Research Report
  • [Presentation] Inhibition of hyaluronan synthesis in breast cancer cells suppresses tumorigenecity in vitro and metastatic lesion of bone in vivo.2010

    • Author(s)
      Urakawa H, Nishida Y, Wasa J, Arai E, Zhuo L, Kimata K, Kozawa E, Futamura N, Ishiguro N
    • Organizer
      HA2010
    • Place of Presentation
      ブライトンホテル京都
    • Related Report
      2010 Annual Research Report
  • [Presentation] Hyaluronan plays significant roles not only in maintenance of musculoskeletal systems but also in tumorigenecity of musculoskeletal malignant tumors2010

    • Author(s)
      Nishida Y, Wasa J, Urakawa H, Arai E, Shinomura T, Zhuo L, Kimata K, Knudson W, Knudson C, Ishiguro N
    • Organizer
      HA2010
    • Place of Presentation
      ブライトンホテル京都
    • Related Report
      2010 Annual Research Report
  • [Presentation] マウス肝障害における好中球細胞浸潤はSHAP-ヒアルロン酸複合体を要求する2009

    • Author(s)
      卓麗聖, 横地高志, 木全弘治
    • Organizer
      糖鎖科学名古屋拠点第7回若手のカフォーラム
    • Place of Presentation
      名古屋大学VBLホール(名古屋)
    • Year and Date
      2009-09-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] Role of SHAP-hyaluronan complex in ovalbumin-induced airway hyperresponsiveness in mice2009

    • Author(s)
      Zhuo L, Zhu L, Kimata K, Yamaguchi E, Baba K
    • Organizer
      20th International Symposium on Glycoconjugate. Glycans : From Molecules to Structures to Therapeutics
    • Place of Presentation
      Puerto Rico
    • Related Report
      2011 Final Research Report
  • [Presentation] 咳喘息患者の気管支粘膜におけるヒアルロン酸の局在について2009

    • Author(s)
      西村眞樹, 馬場研二, 河合聖子, 田中博之, 横江徳仁, 高橋大輔, 八木健郎, 山口悦郎, 塩野裕之, 卓麗聖
    • Organizer
      第59回日本アレルギー学会秋季学術大会
    • Place of Presentation
      秋田キャッスルホテル
    • Related Report
      2011 Final Research Report
  • [Presentation] Formation of SHAP-hyaluronan covalent complex prevents the development of airway hyperresponsiveness in mice2009

    • Author(s)
      Zhuo L, Zhu L, Baba K. Yamaguchi E, Kimata K
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Related Report
      2011 Final Research Report
  • [Presentation] Investigation of influence of glycans on CD44variant forms for cell adhesion mediated by selectin2009

    • Author(s)
      Kanamori A, Zhuo L, Kimata K, Kannagi R
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2011 Final Research Report
  • [Presentation] マウス肝障害における好中球細胞の浸潤はSHAP-ヒアルロン酸複合体を要求する2009

    • Author(s)
      卓麗聖、横地高志、木全弘治
    • Organizer
      糖鎖科学名古屋拠点第7回若手の力フォーラム
    • Place of Presentation
      名古屋
    • Related Report
      2011 Final Research Report
  • [Presentation] SHAP-hyaluronan complex-null mutation enfeebles D-Galactosamine/bacterial lipopolysaccharide-induced liver injury2009

    • Author(s)
      Zhuo L, Yokochi T, Kimata K
    • Organizer
      6th International Conference on Proteoglycan
    • Place of Presentation
      Aixles Bains, France
    • Related Report
      2011 Final Research Report
  • [Presentation] Variability in the chondroitin sulfate linkage structures of bikunin-A potential biological role2009

    • Author(s)
      Lord MS, Youssef P, Zhuo L, Watanabe H, Day A, Whitelock J
    • Organizer
      6th International Conference on Proteoglycan
    • Place of Presentation
      Aixles Bains, France
    • Related Report
      2011 Final Research Report
  • [Presentation] SHAP-hyaluronan complex suppresses the airway hyperresponsiveness in mice2009

    • Author(s)
      Zhuo L, Zhu L, Baba K. Yamaguchi E, Kimata K
    • Organizer
      21st IUBMB & 12th FAOBMB InternationalCongress of Biochemistry and Molecular Biology
    • Place of Presentation
      Shanghai
    • Related Report
      2011 Final Research Report
  • [Presentation] Ovalbumin-induced Airway Hyperresponsiveness is increased in SHAP-hyaluronan complex deficient Mice. Joint Conference of8th PPCTSS2009

    • Author(s)
      Zhuo L, Zhu L, Kimata K, Yamaguchi E, Baba K
    • Organizer
      41st Annual Meeting of JSCTR, 56th Annual Meeting of JMC, and YICCM
    • Place of Presentation
      Shonan Village Center, Kanagawa, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Inter-alpha-trypsin inhibitor ameliorates endotoxin-induced endothelial injury2009

    • Author(s)
      Stober V, Kornepati A, Siryaporn E, Lim Y, Zhuo L, Kimata K, Garantziotis S
    • Organizer
      American Thoracic Society(ATS)
    • Related Report
      2011 Final Research Report
  • [Presentation] Inter-α-tyrpsin inhibitor promotes epithelial recovery after injury through vitronectin binding2009

    • Author(s)
      Adair J, Stober V, Zhuo L, Roberts J, Kimata K, Garantziotis S
    • Organizer
      American Thoracic Society(ATS)
    • Related Report
      2011 Final Research Report
  • [Presentation] Role of SHAP-hyaluronan complex in ovalbumin-induced airway hyperresponsiveness in mice2009

    • Author(s)
      Zhuo L, Zhu L, Kimata K, Yamaguchi E, Baba K
    • Organizer
      20th International Symposium on Glycoconjugate
    • Place of Presentation
      San Juan, Puerto Rico
    • Related Report
      2009 Annual Research Report
  • [Presentation] 咳喘息患者の気管支粘膜におけるピアルロン酸の局在について2009

    • Author(s)
      西村眞樹, 馬場研二, 河合聖子, 田中博之, 横江徳仁, 高橋大輔, 八木健郎, 山口悦郎, 塩野裕之, 卓麗聖
    • Organizer
      第59回日本アレルギー学会秋季学術大会
    • Place of Presentation
      キャッスルホテル(秋田)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Investigation of influence of glycans on CD44 variant forms for cell adhesion mediated by selectin2009

    • Author(s)
      Kanamori A, Zhuo L, Kimata K, Kannagi R
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      国際会議場(神戸)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Formation of SHAP-hyaluronan covalent complex prevents the development of airway hyperresponsiveness in mice2009

    • Author(s)
      Zhuo L, Zhu L, Baba K.Yamaguchi E, Kimata K
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      国際会議場(神戸)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Variability in the chondroitin sulfate linkage structures of bikunin-A potential biological role2009

    • Author(s)
      Lord MS, Youssef P, Zhuo L, Watanabe H, Day A, Whitelock J
    • Organizer
      6th International Conference on Proteoglycan
    • Place of Presentation
      Aix les Bains, France
    • Related Report
      2009 Annual Research Report
  • [Presentation] SHAP-hyaluronan complex-null mutation enfeebles D-Galactosamine/bacterial lipopolysaccharide-induced liver injury2009

    • Author(s)
      Zhuo L, Yokochi T, Kimata K.
    • Organizer
      6th International Conference on Proteoglycan
    • Place of Presentation
      Aix les Bains, France
    • Related Report
      2009 Annual Research Report
  • [Presentation] SHAP-hyaluronan complex suppresses the airway hyperresponsiveness in mice2009

    • Author(s)
      Zhuo L, Zhu L, Baba K.Yamaguchi E, Kimata K.
    • Organizer
      21st IUBMB & 12th FAOBMB InternationalCongress of Biochemistry and Molecular Biology
    • Place of Presentation
      Shanghai, China
    • Related Report
      2009 Annual Research Report
  • [Presentation] Ovalbumin-induced Airway Hyperresponsiveness is increased in SHAP-hyaluronan complex deficient Mice2009

    • Author(s)
      Zhuo L, Zhu, Kimata K, Yamaguchi E, Baba K
    • Organizer
      Joint Conference of 8th Pan-Pacific Connective Tissue Society Symposium, 41st Annual Meeting of the Japanese Society of Connective Tissue Research, 56th Annual Meeting of the Japan Matrix Club, Yokosuka International Conference on Cancer Microenvironments(PPCTSS/JSCTR/JMC/YICCM)
    • Place of Presentation
      Shonan Village Center, Kanagawa
    • Related Report
      2009 Annual Research Report
  • [Book] ますます重要になる細胞周辺環境の科学技術-細胞の生存、増殖、機能のコントロールから再生医療まで2009

    • Author(s)
      卓麗聖、木全弘治
    • Publisher
      株式会社メディカルドゥ
    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] ビクニン遺伝子のターゲッティング用DNA2010

    • Inventor(s)
      卓麗聖、米田雅彦、木全弘治
    • Industrial Property Rights Holder
      生化学工業株式会社
    • Acquisition Date
      2010-08-13
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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