Functional of a brain-specific mucin-type carbohydrate glycosyltransferase involved in neural development and apoptosis

• Project/Area Number: 21570148
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Kyoto Sangyo University
• Principal Investigator: KUROSAKA Akira 京都産業大学, 総合生命科学部, 教授 (90186536)
• Co-Investigator(Kenkyū-buntansha): TERACHI Toru 京都産業大学, 総合生命科学部, 教授 (90202192) ; SEO Misuzu 京都産業大学, 総合生命科学部, 教授 (60211223) ; NAKAMURA Naosuke 京都産業大学, 工学部, 講師 (30424964) ; NAKAYAMA Yoshiaki 京都産業大学, 総合生命科学部, 助教 (40512455)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 糖鎖生物学 / 神経科学 / 糖転移酵素 / アポトーシス / RNAi / ゼブラフィッシュ / マクロピノサイトーシス / 神経発生

Research Abstract

The purpose of this study is to clarify the roles of mucin-type carbohydrates and glycosyltransferases involved in their biosynthesis in neural development. We investigated the functions of WBSCR17, a brain-specific isozyme, and mucin-type carbohydrates in mouse embryonic carcinoma P19 cells, which can differentiate into neural cells, and human embryonic kidney HEK293T cells. Also the roles of WBSCR17 in neural differentiation in zebrafish were examined. Through these studies, we elucidated that WBCSR17 is involved in the neural development and membrane trafficking in the cells.

Research Products

• [Journal Article] Neucrin, a novel secreted antagonist of canonical Wnt signaling, plays roles in developing neural tissues in zebrafish (2012)
  • Author(s): Ayumi Miyake, Satoka Nihno, Yuino Murakoshi, Ayano Satsuka, Yoshiaki Nakayama, and Nobuyuki Itoh
  • Journal Title: Mech Volume: 128 Pages: 577-590
  • Peer Reviewed
• [Journal Article] Neucrin, a novel secreted antagonist of canonical Wnt signaling, plays roles in developing neural tissues in zebrafish (2012)
  • Author(s): A.Miyake
  • Journal Title: Mechanism of Development Volume: 128 Pages: 577-590
  • Peer Reviewed
• [Journal Article] Neuferricin, a novel extra cellular heme-binding protein promotes neurogenesis (2010)
  • Author(s): I. Kimura, Y. Nakayama, M. Konishi, T. Kobayashi, M. Mori, M. Ito, A. Hirasawa, G. Tsujimoto, M. Ohta, N. Itoh, M. Fujimoto
  • Journal Title: J. Neurochem Volume: 112 Issue: 5 Pages: 1155-1167
  • DOI: 10.1111/j.1471-4159.2009.06522.x
  • Peer Reviewed
• [Journal Article] Expression and function of glycogen synthase kinase-3 in human hair follicles (2010)
  • Author(s): Koichi Yamauchi and Akira Kurosaka
  • Journal Title: Arch. Dermatol. Res Volume: 302 Issue: 4 Pages: 263-270
  • DOI: 10.1007/s00403-009-0987-x
  • Peer Reviewed
• [Journal Article] Expression and function of glycogen synthase kinase-3 in human hair follicles (2010)
  • Author(s): Yamauchi, K.
  • Journal Title: Arch.Dermatol.Res. Volume: 302 Pages: 263-270
  • Peer Reviewed
• [Journal Article] Protein O-mannosylation in animal development and physiology : from human disorders to Drosophila phenotypes (2010)
  • Author(s): Nakamura, N.
  • Journal Title: Semin.Cell Dev.Biol. Volume: 21 Pages: 622-630
  • Peer Reviewed
• [Journal Article] Drosophila Dystroglycan is a target of O-mannosyltransferase activity of two protein O-mannosyltransferases, Rotated Abdomen and Twisted (2010)
  • Author(s): Nakamura, N.
  • Journal Title: Glycobiology Volume: 20 Pages: 381-394
  • Peer Reviewed
• [Journal Article] Neuferricin, a novel extracellular heme-binding protein, promotes neurogenesis. (2010)
  • Author(s): Nakayama, Y.
  • Journal Title: J.Neurochem. Volume: 112 Pages: 1156-1167
  • Peer Reviewed
• [Journal Article] Inhibition of glycogen synthase kinase-3 enhances the expression of alkaline phosphatase and insulin-like growth factor-1 in human primary dermal papilla cell culture and maintains mouse hair bulbs in organ culture (2009)
  • Author(s): Koichi Yamauchi and Akira Kurosaka
  • Journal Title: Arch. Dermatol. Res Volume: 301 Issue: 5 Pages: 357-365
  • DOI: 10.1007/s00403-009-0929-7
  • Peer Reviewed
• [Journal Article] Expression and function of glycogen synthase kinase-3 in human hair follicles. (2009)
  • Author(s): Yamauchi, K., Kurosaka, A.
  • Journal Title: Arch. Dermatol. Res.(published on line)
  • Peer Reviewed
• [Presentation] Roles of mucin-type carbohydrates in endocytosis (2012)
  • Author(s): Yoshiaki Nakayama, and Akira Kurosaka
  • Organizer: 2012 Joint Seminar in Science and Technology
  • Place of Presentation: Bangkok Thailand
  • Year and Date: 2012-03-12
• [Presentation] Roles of mucin-type carbohydrates in zebrafish development (2012)
  • Author(s): Akira Kurosaka, and Yoshiaki Nakayama
  • Organizer: 2012 Joint Seminar in Science and Technology
  • Place of Presentation: Bangkok Thailand
  • Year and Date: 2012-03-12
• [Presentation] Roles of mucin-type carbohydrates in zebrafish development (2012)
  • Author(s): A.Kurosaka
  • Organizer: 2012 Joint Seminar in Science and Technology
  • Place of Presentation: Bangkok (Thailand)(招待講演)
  • Year and Date: 2012-03-12
• [Presentation] Roles of mucin-type carbohydrates in endocytosis (2012)
  • Author(s): Y.Nakayama
  • Organizer: 2012 Joint Seminar in Science and Technology
  • Place of Presentation: Bangkok (Thailand)(招待講演)
  • Year and Date: 2012-03-12
• [Presentation] Developmental Roles of Putative Polypeptide GalNAc-transferases in Zebrafish (2011)
  • Author(s): Naosuke Nakamura, Eiichi Kaneda, Yoshiaki Nakayama, and Akira Kurosaka
  • Organizer: 2011 Annual Conference of the Society for Glycobiology
  • Place of Presentation: Seattle, Washington USA
  • Year and Date: 2011-11-11
• [Presentation] Developmental roles of putative polypeptide GalNAc-transferases in zebrafish (2011)
  • Author(s): N.Nakamura
  • Organizer: 2011 Annual Conference of the Society for Glycobiology
  • Place of Presentation: Seattle (USA)
  • Year and Date: 2011-11-11
• [Presentation] Functional analysis of N-acetylgalactosaminyltransferase 8 in zebrafish (2011)
  • Author(s): Tamiko Kawai, Kenji Ohshita, Asaka Kakii, Naosuke Nakamura, Yoshiaki Nakayama, and Akira Kurosaka
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市
• [Presentation] Functional analysis of N-acetylgalactosaminyltransferase-like 4 in zebrafish (2011)
  • Author(s): Naosuke Nakamura, Eiichi Kaneda, Yoshiaki Nakayama, and Akira Kurosaka
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市
• [Presentation] Characterization of a brain-specific polypeptide N-acetylgalactosaminyltransferase (2011)
  • Author(s): Hiroki Fujiwara, Tatsunori Satoh, Yasuhiro Tsuji, Yoshiaki Nakayama, Naosuke Nakamura, and Akira Kurosaka
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市
• [Presentation] A putative polypeptide GalNAc-transferase, WBSCR17, regulates endocytosis in HEK293T cells (2011)
  • Author(s): Yoshiaki Nakayama, Ayumi Wada, Naosuke Nakamura, and Akira Kurosaka
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市
• [Presentation] The Biological Roles of a putative Polypeptide GalNAc-transferase/WBSCR17 (2011)
  • Author(s): Yoshiaki Nakayama, Naosuke Nakamura, and Akira Kurosaka
  • Organizer: Glyco21
  • Place of Presentation: Vienne Austria
• [Presentation] The biological roles of a putative polypeptide GalNAc-transferase/WBSCR17 (2011)
  • Author(s): Y.Nakayama
  • Organizer: Glyco21
  • Place of Presentation: Vienne (Austria)
• [Presentation] The Biological Roles of WBSCR17, a Gene Homologous to Polypeptide GalNAc-transferases in Zebrafish (2010)
  • Author(s): Nakamura, N.
  • Organizer: 第33回日本分子生物学会/第83回日本生化学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] Characterization of Brain-specific Polypeptide GalNAc-transferases in P19 cells (2010)
  • Author(s): Tsuji, Y.
  • Organizer: 第33回日本分子生物学会/第83回日本生化学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] 脳特異的ポリペプチドN-アセチルガラクトサミン転移酵素関連遺伝子の機能解析 (2010)
  • Author(s): 藤原浩喜
  • Organizer: 第33回日本分子生物学会/第83回日本生化学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] Zebrafish as a Model to Study the Developmental Roles of Brain-specific Polypeptide GalNAc-transferase (2010)
  • Author(s): Nakamura, N.
  • Organizer: 2010 Annual Conference of the Society for Glycobiology
  • Place of Presentation: St.Peters Beach, Florida USA
  • Year and Date: 2010-11-05
• [Presentation] Suppression of a novel brain-specific UDP-GalNAc : polypeptide N-acetyl galactosaminyltransferase causes cell death in P19 embryonic carcinoma cells during neural differentiation (2010)
  • Author(s): Kurosaka, A.
  • Organizer: International Carbohydrate Symposium (ICS2010)
  • Place of Presentation: 千葉
  • Year and Date: 2010-08-05
• [Presentation] The Biological Roles of Brain-specific Polypeptide GalNAc-transferases in Zebrafish (2010)
  • Author(s): Nakamura, N.
  • Organizer: International Carbohydrate Symposium (ICS2010)
  • Place of Presentation: 千葉
  • Year and Date: 2010-08-02
• [Presentation] Characterization of brain-specific polypeptide GalNAc-transferases in P19 cells (2010)
  • Author(s): Yoshiaki Nakayama, Yasuhiro Tsuji, Naosuke Nakamura, and Akira Kurosaka
  • Organizer: 第33回日本分子生物学会年会/第83回日本生化学会大会合同年会
  • Place of Presentation: 神戸市
• [Presentation] Functional analysis of brain-specific polypeptide N-acetylglactosaminyltransferaserelated genes (2010)
  • Author(s): Hiroki Fujiwara, Tatsunori Satoh, Yoshiaki Nakayama, Naosuke Nakamura, and Akira Kurosaka
  • Organizer: 第33回日本分子生物学会年会/第83回日本生化学会大会合同年会
  • Place of Presentation: 神戸市
• [Presentation] The Biological Roles of WBSCR17, a Gene Homologous to Polypeptide GalNAc-transferases in Zebrafish (2010)
  • Author(s): Naosuke Nakamura, Masaaki Tawara, Kazuma Nishimura, Kosuke Hachiga, Hirotaka Nishizaki, Yoshiaki Nakayama, Ayumi Miyake, Nobuyuki Itoh, and Akira Kurosaka
  • Organizer: 第33回日本分子生物学会年会/第83回日本生化学会大会合同年会
  • Place of Presentation: 神戸市
• [Presentation] Polypeptide N-acetylgalactosaminyltransferase causes cell death in P19 embryonic carcinoma cells during neural differentiation (2010)
  • Author(s): Akira Kurosaka, Shinya Toba, Tatsunori Satoh, Naosuke Nakamura, Yoshiaki Nakayama, and Kei-ich Ozaki
  • Organizer: International Carbohydrate Symposium
  • Place of Presentation: 横浜市
• [Presentation] The Biological Roles of Brain-specific Polypeptide GalNAc-transferases in Zebrafish (2010)
  • Author(s): Naosuke Nakamura, Masaaki Tawara, Kazuma Nishimura, Kosuke Hachiga, Hirotaka Nishizaki, Yoshiaki Nakayama, Ayumi Miyake, Nobuyuki Itoh, and Akira Kurosaka
  • Organizer: International Carbohydrate Symposium
  • Place of Presentation: 横浜市
• [Presentation] ゼブラフィッシュを用いた神経発生における脳特異的GalNAc転移酵素の機能解析 (2009)
  • Author(s): 西嵜大貴
  • Organizer: 第82回 日本生化学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2009-10-24
• [Book] Human Genetic Diseases (2011)
  • Author(s): Sayaka Sasaki, Hiroya Ohta, Yoshiaki Nakayama, Morichika Konishi, Ayumi Miyake, and Nobuyuki Itoh
  • Publisher: In Tech
• [Book] Human Genetic Diseases (2011)
  • Author(s): S.Sasaki
  • Publisher: In Tech
• [Remarks]
  • URL: http://www.cc.kyoto-su.ac.jp/-kurosaka/
• [Remarks]
  • URL: http://www.cc.kyoto-su.ac.jp/~kurosaka/
• [Remarks]
  • URL: http://post.kyoto-su.ac.jp/s/w020/w020a100-utf.php?sno=2040