Does inhibition of phosphatidylserine synthase induce autophagy?

• Project/Area Number: 21570155
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: TANIDA Isei 国立感染症研究所, 細胞化学部, 室長 (30296868)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: オートファジー / リン脂質代謝 / タンパク質分解 / 細胞死 / ホスファチジルセリ / タンパク分解 / ホスファチジルセリン / LC3 / ATG / PSS1 / PSS2

Research Abstract

To investigate the relation between a defect in phosphatidylserine synthesis and autophagic cell death, we focused on a CHO mutant that has a defect in an activity of phosphatidylserine synthase. When the mutant cells were cultured in a medium lacking phosphatidylserine, LC3-II, a promising autophagic marker, was increased within 24h. At 24-48h after induction of LC3-II, cell viability was decreased. The results suggested that the autophagy play a cytoprotective role, when phsophatidylserine depeletion-induced cell death.

Research Products

• [Journal Article] The FAP motif within human ATG7, an autophagy-related E1-like enzyme, is essential for the E2-substrate reaction of LC3 lipidation (2012)
  • Author(s): Tanida I, Yamasaki M, Komatsu M, Ueno T.
  • Journal Title: Autophagy Volume: 8 Issue: 1 Pages: 88-97
  • DOI: 10.4161/auto.8.1.18339
  • Peer Reviewed
• [Journal Article] Doxorubicin-induced glomerulosclerosis with proteinuria in GFP-GABARAP transgenic mice (2012)
  • Author(s): Takagi-Akiba M, Asanuma K, Tanida I, Tada N, Oliva Trejo JA, Nonaka K, Asanuma E, Kominami E, Ueno T, Tomino Y.
  • Journal Title: Am J Physiol Renal Physiol Volume: 302 Issue: 3 Pages: F380-F389
  • DOI: 10.1152/ajprenal.00502.2010
  • Peer Reviewed
• [Journal Article] Hepatic steatosis inhibits autophagic proteolysis via impairment of autophagosomal acidification and cathepsin expression (2011)
  • Author(s): Inami Y, Yamashina S, et al
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 412 Issue: 4 Pages: 618-25
  • DOI: 10.1016/j.bbrc.2011.08.012
  • Peer Reviewed
• [Journal Article] Liver autophagy contributes to the maintenance of blood glucose and amino acid levels (2011)
  • Author(s): Ezaki, J., Matsumoto, N., Takeda-Ezaki, M., Komatsu, M., Takahashi, K., Hiraoka, Y., Taka, H., Fujimura, T., Takehana, K., Yoshida, M., Iwata, J., Tanida, I., Furuya, N., Zheng, D-m., Tada, N., Tanaka, K., Kominami, E., Ueno, T.
  • Journal Title: Autophagy Volume: 7 Issue: 7 Pages: 727-736
  • DOI: 10.4161/auto.7.7.15371
  • Peer Reviewed
• [Journal Article] Caffeine induces apoptosis by enhancement of autophagy via PI3K/Akt/mTOR/p70S6K inhibition (2011)
  • Author(s): Saiki S
  • Journal Title: Autophagy Volume: 7(2) Issue: 2 Pages: 176-87
  • DOI: 10.4161/auto.7.2.14074
  • Peer Reviewed
• [Journal Article] Autophagy basics (2011)
  • Author(s): Tanida I.
  • Journal Title: Microbiol Immunol Volume: 55 Issue: 1 Pages: 1-11
  • DOI: 10.1111/j.1348-0421.2010.00271.x
  • NAID: 10031121434
  • Peer Reviewed
• [Journal Article] Autophagosome Formation and Molecular Mechanism of Autophagy (2011)
  • Author(s): Tanida I.
  • Journal Title: Antioxid Redox Signal Volume: 14 Issue: 11 Pages: 2201-14
  • DOI: 10.1089/ars.2010.3482
  • Peer Reviewed
• [Journal Article] オートファジー-リソソーム分解系～疾患・生理機能との関わりと分子機構 (2011)
  • Author(s): 谷田以誠
  • Journal Title: 内分泌・糖尿病・代謝内科、和文総説 Volume: 33 Pages: 319-325
  • URL: http://www.kahyo.com/item/B201110-334
• [Journal Article] オートファジーの評価方法、実験医学 (2011)
  • Author(s): 谷田以誠、上野隆、和栗聡
  • Journal Title: 和文総説 Volume: 29 Pages: 2054-2062
  • URL: http://www.yodosha.co.jp/bookdata/9784758103169/9784758103169_contents.pdf
• [Journal Article] Measurement of autophagy in cells and tissues (2010)
  • Author(s): Tanida I, Waguri S.
  • Journal Title: Methods Mol Biol Volume: 648 Pages: 193-214
  • DOI: 10.1007/978-1-60761-756-3_13
  • ISBN: 9781607617556, 9781607617563
  • Peer Reviewed
• [Journal Article] Autophagic compartments gain access to the MHC class II compartments in thymic epithelium (2009)
  • Author(s): Kasai M, Tanida I, Ueno T, Kominami E, Seki S, Ikeda T, Mizuochi T.
  • Journal Title: J Immunol Volume: 183 Pages: 7278-7285
  • URL: http://www.jimmunol.org/content/183/11/7278.long
  • Peer Reviewed
• [Journal Article] Knockdown of autophagy-related gene decreases the production of infectious hepatitis C virus particles (2009)
  • Author(s): Tanida I, Fukasawa M, Ueno T, Kominami E, Wakita T, Hanada K.
  • Journal Title: Autophagy Volume: 5 Pages: 937-945
  • URL: http://www.landesbioscience.com/journals/autophagy/article/9243/?nocache=162694941
  • Peer Reviewed
• [Journal Article] Inhibition of hepatitis C virus replication by chloroquine targeting virus-associated autophagy (2009)
  • Author(s): Mizui T, Yamashina S, Tanida I, Takei Y, Ueno T, Sakamoto N, Ikejima K, Kitamura T, Enomoto N, Sakai T, Kominami E, Watanabe S.
  • Journal Title: J Gastroenterol Volume: 45 Issue: 2 Pages: 195-203
  • DOI: 10.1007/s00535-009-0132-9
  • NAID: 10027214573
  • Peer Reviewed
• [Presentation] Ubiquitin modifies an autophagic modifier to inhibit ALIPS formation (2012)
  • Author(s): Tanida, I., Koike, M., Ueno, T., Uchiyama, Y.
  • Organizer: Gordon Research Conferences "Autophay in Stress, Development and Disease"
  • Place of Presentation: Ventura, CA, USA
• [Presentation] Ubiquitin modifies an autophagic modifier to inhibit ALIPS formation (2012)
  • Author(s): I.Tanida
  • Organizer: Gordon Research Conference 2012 "Autophagy In Stress
  • Place of Presentation: ベンチュラ(米国・カリフォルニア)
• [Presentation] 新奇構造体ALIPS形成抑制のためのユビキチン・プロテアソームによるATG12分解制御 (2011)
  • Author(s): 谷田以誠、小池正人、上野隆、内山安男
  • Organizer: 第34回日本分子生物学会年
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15
• [Presentation] 新奇構造体ALIPS形成抑制のためのユビキチン・プロテアソームによるATG12分解制御 (2011)
  • Author(s): 谷田以誠, ら
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15
• [Presentation] Stability of luciferase activity during autophagy (2010)
  • Author(s): Tanida, I., Hanada, K.
  • Organizer: 50th Annual Meeting of American Society of Cell Biology
  • Place of Presentation: Philadelphia, PA, U. S. A.
• [Presentation] オートファジー条件におけるプロテアソームの活性化 (2010)
  • Author(s): 谷田以誠、花田賢太郎
  • Organizer: BMB2010
  • Place of Presentation: 神戸
• [Presentation] Autophagic Mechanism and Production of HCV Particles (2010)
  • Author(s): Tanida, I.
  • Organizer: World Congress of Virus and Infection-2010
  • Place of Presentation: Busan, Korea
• [Presentation] Autophagy and mTor-signaling in production of infectious HCV-particles (2010)
  • Author(s): Tanida, I., Fukasawa, M., Ueno, T., Kominami, E., Wakita, T., Hanada, K.
  • Organizer: Gordon Research Conferences "Autophay in Stress, Development and Disease", Il Ciocco
  • Place of Presentation: Barga, Italy
• [Presentation] Visualization of GABARAP in mice (2009)
  • Author(s): Tanida, I., Koike, M., Tada, N., Iwata, J., Ueno, T., Uchiyama, Y., Kominami, E., Hanada, K.
  • Organizer: 49th Annual Meeting of American Society of Cell Biology
  • Place of Presentation: San Diego, U. S. A.
  • Year and Date: 2009-12-08
• [Presentation] オートファジーはHCV粒子産生に関与している (2009)
  • Author(s): 谷田以誠、深澤征義、脇田隆字、花田賢太郎
  • Organizer: 第57回日本ウィルス学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-25
• [Presentation] オートファジー関連遺伝子のノックダウンはHCV粒子産生を抑制する (2009)
  • Author(s): 谷田以誠、深澤征義、脇田隆字、上野隆、木南英紀、花田賢太郎
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Presentation] Autophagy in Naive Hepatitis C Virus Production (2009)
  • Author(s): Tanida, I.
  • Organizer: 5th International Symposium on Autophagy
  • Place of Presentation: Shiga, Japan
  • Year and Date: 2009-09-27
• [Presentation] Essential role of liver autophagy in maintaining blood glucose and plasma amino acids (2009)
  • Author(s): Ueno, T., Ezaki, J., Matsumoto, N., Takeda-Ezaki, M., Komatsu, M., Fujimura, T., Tanida, I., Furuya, N., Kominami, E.
  • Organizer: 5th International Symposium on Autophagy
  • Place of Presentation: Shiga, Japan
  • Year and Date: 2009-09-27
• [Presentation] Autophagy Basics (2009)
  • Author(s): Tanida, I.
  • Organizer: International Symposium on Autophagy and Cell Death
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2009-07-06
• [Remarks]
  • URL: http://www.nih.go.jp/niid/ja/from-biochem/931-2nd.html
• [Remarks]
  • URL: http://www.nih.go.jp/niid/ja/from-biochem/931-2nd.html
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  • URL: http://www.nih.go.jp/niid/biochem/2nd/index.html
• [Remarks]
  • URL: http://www.nih.go.jp/niid/biochem/2nd/index.html