Collective migration of epithelial cells induced by softness of substrates

• Project/Area Number: 21570158
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Hokkaido University
• Principal Investigator: HAGA Hisashi 北海道大学, 大学院・先端生命科学研究院, 准教授 (00292045)
• Co-Investigator(Kenkyū-buntansha): KAWABATA Kazushige 北海道大学, 大学院・先端生命科学研究院, 教授 (20261274)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 上皮細胞 / 細胞運動 / 集団性 / メカノセンス / コラーゲンゲル / 接着構造 / 集団運動 / ゲル基質

Research Abstract

Cell movement plays an important role in many physiological processes, including wound healing, embryonic development, and morphogenesis. In this study, we observed that epithelial cells (Madin-Darby canine kidney cell) migrated collectively along one direction on a soft substrate. Cell-substrate adhesions, cell-cell mechanotransduction, and appearance of leader cells were studied.

Research Products

• [Journal Article] Interruption of glycosphingolipid synthesis enhances osteoarthritis development in mice (2012)
  • Author(s): N.Seito, et al.
  • Journal Title: Arthritis & Rheumatism Volume: (印刷中) Issue: 8 Pages: 2579-88
  • DOI: 10.1002/art.34463
  • Peer Reviewed
• [Journal Article] Active fluctuation in the cortical cytoskeleton observed by high-speed live-cell scanning probe microscopy (2011)
  • Author(s): K.Tamura, T.Mizutani, H.Haga, K.Kawabata
  • Journal Title: Acta Biomater. Volume: 7 Issue: 10 Pages: 3766-3772
  • DOI: 10.1016/j.actbio.2011.06.013
  • Peer Reviewed
• [Journal Article] Radiation-induced Cancer Cell Repopulation : A Possible Mechanism Implied by Experiments Using Transplantable Mouse-derived Sarcoma Cell Line (2011)
  • Author(s): T.Nishioka, et al.
  • Journal Title: Cell Structure and Function Volume: 36 Pages: 13-20
  • Peer Reviewed
• [Journal Article] Nano-mechanical Properties of Living Cells Expressing Constitutively Active RhoA Effectors (2010)
  • Author(s): K. Tamura, T. Mizutani, H. Haga, and K. Kawabata
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 403巻 Issue: 3-4 Pages: 363-367
  • DOI: 10.1016/j.bbrc.2010.11.036
  • Peer Reviewed
• [Journal Article] Integrin β1-dependent Invasive Migration of Irradiation-tolerant Human Lung Adenocarcinoma Cells in 3D Collagen Matrix (2010)
  • Author(s): S.Ishihara, et al.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 396 Pages: 651-655
  • Peer Reviewed
• [Journal Article] Nano-mechanical Properties of Living Cells Expressing Constitutively Active RhoA Effectors (2010)
  • Author(s): K.Tamura, et al.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 403 Pages: 363-367
  • Peer Reviewed
• [Journal Article] Precise Synthesis and Physicochemical Properties of High-Density Polymer Brushes Designed with Poly (N-isopropylacrylamide) (2010)
  • Author(s): H.Suzuki, et al.
  • Journal Title: Macromolecules Volume: 43 Pages: 9945-9956
  • Peer Reviewed
• [Journal Article] Dynamics of Leading Lamellae of Living Fibroblasts Visualized by High-speed Scanning Probe Microscopy (2010)
  • Author(s): K.Tamura, et al.
  • Journal Title: Histochem. Cell Biol. 133 Pages: 59-67
  • Peer Reviewed
• [Journal Article] Regulation of Cellular Contractile Force in Response to Mechanical Stretch by Diphosphorylation of Myosin Regulatory Light Chain via RhoA Signaling Cascade (2009)
  • Author(s): T. Mizutani, K. Kawabata, Y. Koyama, M. Takahashi, and H. Haga
  • Journal Title: Cell Motility and the Cytoskeleton Volume: 66巻 Issue: 7 Pages: 389-397
  • DOI: 10.1002/cm.20378
  • Peer Reviewed
• [Journal Article] Increased Motility and Invasiveness of Tumor Cells that Survived 10Gy Irradiation (2009)
  • Author(s): K.Tsutsumi, et al.
  • Journal Title: Cell Structure and Function 34 Pages: 89-96
  • Peer Reviewed
• [Journal Article] Regulation of Cellular Contractile Force in Response to Mechanical Stretch by Diphosphorylation of Myosin Regulatory Light Chain via RhoA Signaling Cascade (2009)
  • Author(s): T.Mizutani, et al.
  • Journal Title: Cell Motil. Cytoskeleton 66 Pages: 389-397
  • Peer Reviewed
• [Presentation] 3D Morphogenesis of Epithelial Cells on a Laminin-rich Soft Substrate Induced by Cellular Contractile Force (2011)
  • Author(s): M.Imai
  • Organizer: 第51回米国細胞生物学会年会
  • Place of Presentation: コロラドコンベンションセンター(アメリカ合衆国)
  • Year and Date: 2011-12-05
• [Presentation] NF-kB expression in cancer cells regulated by substrate stiffness (2011)
  • Author(s): 石原誠一郎
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2011-10-03
• [Presentation] 軟らかい基質がもたらす上皮細胞集団の協調運動 (2011)
  • Author(s): 芳賀永
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川市大雪クリスタルホール
  • Year and Date: 2011-09-23
• [Presentation] 軟らかい基質がもたらす上皮細胞集団の協調運動 (2011)
  • Author(s): 芳賀永
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川市大雪クリスタルホール(北海道)(招待講演)
  • Year and Date: 2011-09-23
• [Presentation] ラミニンを含む軟らかいゲル基質上でみられる上皮細胞集団の3次元形態形成 (2011)
  • Author(s): 今井美沙子
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2011-06-29
• [Presentation] Collective behavior of epithelial cells responding to stiffness of the extracellular matrix (2011)
  • Author(s): 芳賀永
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2011-06-27
• [Presentation] Collective behavior of epithelial cells responding to stiffness of the extracellular matrix (2011)
  • Author(s): 芳賀永
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道大学(札幌市)(招待講演)
  • Year and Date: 2011-06-27
• [Presentation] 細胞外基質の硬さ・表面形状・組成に応答する上皮細胞の集団協調運動 (2011)
  • Author(s): 芳賀永
  • Organizer: 口腔先端応用医科学研究会第3回学術会議
  • Place of Presentation: 東京医科歯科大学
  • Year and Date: 2011-01-22
• [Presentation] 細胞外基質の硬さ・表面形状・組成に応答する上皮細胞の集団協調運動 (2011)
  • Author(s): 芳賀永
  • Organizer: 口腔先端応用医科学研究会第3回学術会議
  • Place of Presentation: 東京医科歯科大(東京都) 招待講演
  • Year and Date: 2011-01-22
• [Presentation] Enhancement of NF-κB Expression and Malignancy of Cancer Cells Induced by Substrate Stiffness (2010)
  • Author(s): S.Ishihara
  • Organizer: 第50回米国細胞生物学会年会
  • Place of Presentation: 米国フィラデルフィア市・コンベンションセンター
  • Year and Date: 2010-12-13
• [Presentation] 上皮細胞の集団運動と3次元形態形成 (2010)
  • Author(s): 芳賀永
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-12-08
• [Presentation] 上皮細胞の集団運動と3次元形態形成 (2010)
  • Author(s): 芳賀永
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場(神戸市) 招待講演
  • Year and Date: 2010-12-08
• [Presentation] 基質の硬さがもたらす転写因子NF-κB田の発現 (2010)
  • Author(s): 石原誠一郎
  • Organizer: 第5回メカノセンシング研究会
  • Place of Presentation: 京都大学(京都市)
  • Year and Date: 2010-11-26
• [Presentation] 培養基質の硬さ、表面形状がもたらす細胞集団の3次元形態形成 (2010)
  • Author(s): 今井美沙子
  • Organizer: 第5回メカノセンシング研究会
  • Place of Presentation: 京都大学(京都市)
  • Year and Date: 2010-11-26
• [Presentation] 培養基質の硬さがもたらす細胞集団の協調運動と形態形成 (2010)
  • Author(s): 芳賀永
  • Organizer: 第1回バイオエンジニアリングサマースクール
  • Place of Presentation: 北海道医療大学・サテライトキャンパス
  • Year and Date: 2010-09-03
• [Presentation] 培養基質の硬さがもたらす細胞集団の協調運動と形態形成 (2010)
  • Author(s): 芳賀永
  • Organizer: 第1回バイオエンジニアリングサマースクールin Sapporo
  • Place of Presentation: 北海道医療大学・サテライトキャンパス(札幌市) 招待講演
  • Year and Date: 2010-09-03
• [Presentation] ナノパターン基盤の表面形状がもたらす細胞の集団挙動 (2010)
  • Author(s): 今井美沙子
  • Organizer: 第1回バイオエンジニアリングサマースクールin Sapporo
  • Place of Presentation: 北海道医療大学・サテライトキャンパス(札幌市)
  • Year and Date: 2010-09-03
• [Presentation] Invasive Mechanism of Lung Adenocarcinoma Cells Revealed by 3D Collagen Culture (2010)
  • Author(s): S.Ishihara
  • Organizer: 第57回マトリツクス研究会大会
  • Place of Presentation: 秋田拠点センター・アルヴェ(秋田市)
  • Year and Date: 2010-08-19
• [Presentation] Integrin Dependent Invasive Mmigration in 3D Collagen Matrix of Human Lung Adenocarcinoma Cells that Survived 10Gy lirradiation (2010)
  • Author(s): S.Ishihara
  • Organizer: 札幌国際がんシンポジウム2010
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2010-06-28
• [Presentation] Invasive Migration in 3D Collagen of Human Lung Adenocarcinoma Cells that Survived 10Gy Irradiation (2010)
  • Author(s): 石原誠一郎
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 2010-05-19
• [Presentation] 3D Morphogenesis of Epithelial Cells Observed on a Soft Substrate Containing Laminin (2010)
  • Author(s): 今井美沙子
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 2010-05-19
• [Presentation] 細胞外基質の硬さおよび組成に応答する細胞集団の協調挙動と形態形成 (2010)
  • Author(s): 芳賀永
  • Organizer: 北海道医療大学個体差研セミナー
  • Place of Presentation: 北海道医療大学
  • Year and Date: 2010-04-02
• [Presentation] 細胞外基質の硬さおよび組成に応答する細胞集団の協調挙動と形態形成 (2010)
  • Author(s): 芳賀永
  • Organizer: 北海道医療大学個体差研セミナー
  • Place of Presentation: 北海道医療大学(当別町) 招待講演
  • Year and Date: 2010-04-02
• [Presentation] 3D Morphogenesis and Collective Migration of Epithelial Cells Observed on a Soft Substrate Containing Laminin (2009)
  • Author(s): 芳賀永
  • Organizer: 第49回米国細胞生物学会年会
  • Place of Presentation: サンディエゴコンベンションセンター
  • Year and Date: 2009-12-08
• [Presentation] 3D Morphogenesis and Collective Migration of Epithelial Cells Observed on a Soft Substrate Containing Laminin (2009)
  • Author(s): H.Haga, et al.
  • Organizer: 第49回米国細胞生物学会年会
  • Place of Presentation: 米国・サンディエゴ市・コンベンションセンター
  • Year and Date: 2009-12-08
• [Remarks]
  • URL: http://altair.sci.hokudai.ac.jp/g3/member/hagaj.html
• [Remarks]
  • URL: http://altair.sci.hokudai.ac.jp/g3/member/hagai.html