| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
Myosin is motor protein which converts chemical energy into mechanical movement. Phylogenetic analysis revealed that myosins consist of at least 35 classes. Among these classes, plant has only 2 classes, namely, class VIII and class XI. although enzymatic and motile properties of Arabidopsis class VIII myosin, ATM. We have found that actin sliding velocity by ATm was very slow and actin-activated ATPase activity of ATm was very low. In addition, we also found that both the actin sliding velocity and the actin-activated ATPase activities were regulated by free Mg^<2+>. Kinetic analyses using stopped flow apparatus revealed that free Mg^<2+>controled ADP dissociation rate from acto-myosin-ADP.
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