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Characterization of enzymatic property plant specific class VIII myosin

Research Project

Project/Area Number 21570159
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionChiba University

Principal Investigator

ITO Kohji  千葉大学, 大学院・理学研究院, 准教授 (50302526)

Co-Investigator(Kenkyū-buntansha) 岩城 光宏  大阪大学, 大学院・医学研究科, 助教 (30432503)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsミオシン / 分子モーター / アクチン / シロイヌナズナ / 植物 / キネティクス / 遊離マグネシウムイオン / モータータンパク質 / 1分子アッセイ
Research Abstract

Myosin is motor protein which converts chemical energy into mechanical movement. Phylogenetic analysis revealed that myosins consist of at least 35 classes. Among these classes, plant has only 2 classes, namely, class VIII and class XI. although enzymatic and motile properties of Arabidopsis class VIII myosin, ATM. We have found that actin sliding velocity by ATm was very slow and actin-activated ATPase activity of ATm was very low. In addition, we also found that both the actin sliding velocity and the actin-activated ATPase activities were regulated by free Mg^<2+>. Kinetic analyses using stopped flow apparatus revealed that free Mg^<2+>controled ADP dissociation rate from acto-myosin-ADP.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (30 results)

All 2012 2011 2010 2009

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (24 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 2 results)

  • [Journal Article] Unique charge distribution in surface loops confers high velocity on the fast motor protein Chara myosin2009

    • Author(s)
      Ito K, Yamaguchi Y, Yanase K, Ichikawa Y, Yamamoto K
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: Vol.106 Issue: 51 Pages: 21585-21990

    • DOI

      10.1073/pnas.0910787106

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Brownian search-and-catch mechanism for myosin-VI steps2009

    • Author(s)
      M.Iwaki, A.Iwane, T.Shimokawa, R.Cooke, T.Yanagida
    • Journal Title

      Nature Chemical Biology

      Volume: 5 Issue: 6 Pages: 376-377

    • DOI

      10.1038/nchembio.171

    • NAID

      10025976295

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Unique charge distribution in surface loops confers high velocity on the fastmotor protein Chara myosin.2009

    • Author(s)
      Ito K, Yamaguchi Y, Yanase K, Ichikawa Y, Yamamoto K.
    • Journal Title

      Proc Natl Acad Sci USA. 106

      Pages: 21585-21990

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Brownian search-and-catch mechanism for myosin-VI steps.2009

    • Author(s)
      Iwaki M, Iwane AH, Shimokawa T, Cooke R, Yangida T.
    • Journal Title

      Nat Chem Biol. 5

      Pages: 376-377

    • NAID

      10025976295

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] シャジクモ・シロイヌナズナキメラミオシンXIによる原形質流動高速化はシロイヌナズナを大型化する2012

    • Author(s)
      富永基樹, 木村篤司, 山本啓一, 中野明彦, 伊藤光二
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Single molecule analysis of actomyosin motility in the presence of osmolyte2012

    • Author(s)
      M.Iwaki
    • Organizer
      Workshop on "Hydration and ATP energy" 2012
    • Place of Presentation
      仙台
    • Year and Date
      2012-03-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] 浸透圧変調下におけるアクトミオシン運動機能の1分子解析2012

    • Author(s)
      K.Fujita, K.Itoh, A.Iwane, T.Yanagida, M.Iwaki
    • Organizer
      Workshop on "Hydration and ATP energy" 2012
    • Place of Presentation
      仙台
    • Year and Date
      2012-03-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] Switching the force generation model between lever arm swing and Brownian search and catch2012

    • Author(s)
      K.Fujita, M.Iwaki, A.Iwane, L.Marcucci, T.Yanagida
    • Organizer
      Biophysical Society 66th Annual Meeting
    • Place of Presentation
      San Diego, CA, USA
    • Year and Date
      2012-02-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] シロイヌナズナミオシン11は多分子による高速移動に有利である2012

    • Author(s)
      森川高光、伊藤光二、木村篤、富永基樹、池崎圭吾、小森智貴、藤田恵介、中野明彦、山本啓一、岩城光宏、柳田敏雄
    • Organizer
      生体運動研究合同班会議
    • Place of Presentation
      筑波大学、茨城
    • Year and Date
      2012-01-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] 最速ミオシンであるクラスXI車軸藻ミオシンの酵素機能解析2011

    • Author(s)
      伊藤光二, 原口武士, 山本啓一
    • Organizer
      第84回日本生化学学会大会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 高速型シャシクモシロイ ヌナスナキメラミオシンXIかシロイヌナスナ細胞内輸送およひ成長に及ぼす影響2011

    • Author(s)
      富永基樹, 木村篤司, 山本啓一, 中野明彦, 伊藤光二
    • Organizer
      第84回日本生化学学会大会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] 高速型シャジクモ・シロイヌナズナキメラミオシンXI2011

    • Author(s)
      富永基樹, 木村篤司, 山本啓一, 中野明彦, 伊藤光二
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      東京大学
    • Year and Date
      2011-09-18
    • Related Report
      2011 Final Research Report
  • [Presentation] 高速型シャシクモシロイ ヌナスナキメラミオシンXIかシロイヌナスナ細胞内輸送およひ成長に及ぼす影響2011

    • Author(s)
      富永基樹, 木村篤司, 山本啓一, 中野明彦, 伊藤光二
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京大学 駒場キャンパス
    • Year and Date
      2011-09-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] The effect of force and osmotic pressure on the chemo-mechanical transduction of ATP-driven linear motor2011

    • Author(s)
      M.Iwaki
    • Organizer
      The 49th annual meeting of the Biophysical Society of Japan "Asymmetry production by water and ATP"
    • Place of Presentation
      兵庫県立大学姫路書写キャンパス、兵庫
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Switching the force generation model between lever arm swing and Brownian search and catch2011

    • Author(s)
      K.Fujita, M.Iwaki, A.Iwane, L.Marcucci, T.Yanagida
    • Organizer
      The 49th annual meeting of the Biophysical Society of Japan "Asymmetry production by water and ATP"
    • Place of Presentation
      兵庫県立大学姫路書写キャンパス、兵庫
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Arabidopsis Thaliana Myosin XI properties make it ideal for fast transport by multiple myosins2011

    • Author(s)
      T.Morikawa, K.Itoh, M.Iwaki, T.Yanagida
    • Organizer
      The 49th annual meeting of the Biophysical Society of Japan "Asymmetry production by water and ATP"
    • Place of Presentation
      兵庫県立大学姫路書写キャンパス、兵庫
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Co-solvent effect on actomyosin motility revealed by single molecule measurements2011

    • Author(s)
      Mitsuhiro Iwaki
    • Organizer
      「水を主役としたATPエネルギー変換」全体会議
    • Place of Presentation
      大阪ガーデンパレス、大阪
    • Year and Date
      2011-08-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] Switching the force generation model between lever arm swing and Brownian search and catch2011

    • Author(s)
      K.Fujita, M.Iwaki, A.Iwane, L.Marcucci, T.Yanagida
    • Organizer
      「水を主役としたATPエネルギー変換」全体会議
    • Place of Presentation
      大阪ガーデンパレス、大阪
    • Year and Date
      2011-08-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] Direct observation of elementary mechanical process for myosin II2011

    • Author(s)
      M.Iwaki, A.Iwane, T.Yanagida
    • Organizer
      「水を主役としたATPエネルギー変換」全体会議
    • Place of Presentation
      大阪ガーデンパレス、大阪
    • Year and Date
      2011-08-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] Energetics and switching of myosin-V force generation between lever-arm swing and Brownian-secarch-and-catch2011

    • Author(s)
      M. Iwaki, K. Fujita, A. Iwane, L. Marcucci, T. Yanagida
    • Organizer
      Gordon Research Conferences Muscle & Molecular Motors
    • Place of Presentation
      New London, USA
    • Year and Date
      2011-07-10
    • Related Report
      2011 Final Research Report
  • [Presentation] Energetics and switching of myosin-V force generation between lever-arm swing and Brownian-search-and-catch2011

    • Author(s)
      M.Iwaki, K.Fujita, A.Iwane, L.Marcucci, T.Yanagida
    • Organizer
      Gordon Research Conferences "Muscle & Molecular Motors"
    • Place of Presentation
      New London, USA
    • Year and Date
      2011-07-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] シロイヌナズナミオシンXI-Iは、アクチンとの親和性が高く、滑り速度は低い2011

    • Author(s)
      原口武士, 伊藤光二, 山本啓一
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2011-03-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] 高速型シャジクモ・シロイヌナズナキメラミオシンXIがシロイヌナズナ細胞内輸送および成長に及ぼす影響2011

    • Author(s)
      富永基樹, 木村篤司, 山本啓一, 中野明彦, 伊藤光二
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2011-03-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] Arabidpsis thaliana Myosin XI is a processive motor with a broad stepsize2011

    • Author(s)
      T.Morikawa, K.Ito, A.Kimura, K.Ikezaki, T.Komori, K.Fujita, M.Iwaki, T.Yanagida
    • Organizer
      The Biophysical Society's 55th Annual Meeting
    • Place of Presentation
      Baltimore Convention Center(Baltimore, U.S.A.)
    • Year and Date
      2011-03-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Analysis of enzyme properties of Arabidopsis thaliana myosin XI-I.2010

    • Author(s)
      Takashi Haraguchi, Kohji Ito, Keiichi Yamamoto
    • Organizer
      日本生物物理学会第48回年会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] クライオ電子顕微鏡法による車軸藻ミオシンの高速移動分子メカニズムの解明2010

    • Author(s)
      Hiroaki Hidaka, Hiroshi Mine, KohjiIto, Keiichi Yamamoto, Takuo Yasunaga
    • Organizer
      日本生物物理学会第48回年会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 高速型シャジクモ・シロイヌナズナキメラミオシンXIを用いた細胞内交通機構の解析2010

    • Author(s)
      富永基樹, 伊藤光二, 木村篤司, 山本啓一, 中野明彦
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      中部大学(春日井市)
    • Year and Date
      2010-09-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] クラスVIIIミオシンは超high duty ratioである。2009

    • Author(s)
      Ito, K., Matsumoto R., Yamamoto, K.
    • Organizer
      第47回 日本生物物理学会
    • Place of Presentation
      アクティ徳島(徳島市)
    • Year and Date
      2009-10-30
    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] 成長増強植物及びその作出方法2011

    • Inventor(s)
      富永基樹, 伊藤光二
    • Industrial Property Rights Holder
      富永基樹, 伊藤光二, 理化学研究所
    • Filing Date
      2011-06-29
    • Related Report
      2011 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 成長増強植物及びその作出方法2011

    • Inventor(s)
      富永基樹, 伊藤光二
    • Industrial Property Rights Holder
      富永基樹, 伊藤光二, 理化学研究所
    • Filing Date
      2011-06-29
    • Related Report
      2011 Annual Research Report
    • Overseas

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Published: 2009-04-01   Modified: 2016-04-21  

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