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Construction of an atomic model of the skeletal muscle thin filament by FRET measurements

Research Project

Project/Area Number 21570162
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionUniversity of Fukui

Principal Investigator

MIKI Masao  福井大学, 大学院・工学研究科, 教授 (30242580)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsタンパク質 / 核酸の構造 / 動態 / 機能 / アクチン / トロポミオシン / トロポニン / 筋収縮制御 / FRET / 細いフィラメント / カルシウム制御 / アトミックモデル / 生物物理 / 分子スイッチ / 蛍光測定 / 制御メカニズム
Research Abstract

Striated muscle thin filament is composed of actin, tropomyosin(Tm), and troponin(Tn). The fluorescence resonance energy(FRET) donor probe was labeled at the specific sites on tropomyosin or troponin, and the energy acceptor probes were labeled at the specific sites on actin. From these donor and acceptor pairs, FRET efficiencies were determined. Using the atomic coordinates of F-actin, Tm, and Tn, we searched all possible arrangements for Tn and Tm on F-actin to calculate FRET efficiency for each donor-acceptor pair in each arrangement. By minimizing the squared sum of deviations for the calculated FRET efficiencies from the observed efficiencies, we determined the location of Tm and Tn on F-actin.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (21 results)

All 2012 2011 2010 2009

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (13 results)

  • [Journal Article] A Three-Dimensional FRET Analysis to Construct an Atomic Model of the Actin. Tropomyosin. Troponin Core Domain Complex on a Muscle Thin Filament2012

    • Author(s)
      Miki, M., Makimura, S., Sugahara, Y., Yamada, R., Bunya, M., Saitoh, T., & Tobita, H.
    • Journal Title

      J. Mol. Biol

      Volume: 420 Pages: 40-55

    • NAID

      120004186302

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Three Dimensional FRET Analysis to construct an Atomic Model of the Actin-Tropomyosin-Troponin Core Domain Complex on a Muscle Thin Filament2012

    • Author(s)
      Miki, M., Makimura, S., Sugahara, Y., Yamada, R., Bunya, M., Saitoh, T., Tobita, H.
    • Journal Title

      Journal of Molecular Biology

      Volume: 420 Issue: 1-2 Pages: 40-55

    • DOI

      10.1016/j.jmb.2012.03.029

    • NAID

      120004186302

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Three-Dimensional FRET Analysis to Construct an Atomic Model of the Actin-Tropomyosin Complex on a Reconstituted Thin Filament2011

    • Author(s)
      M. Miki, S. Makimura, T. Saitoh, M. Bunya, Y. Sugahara, Y. Ueno, C. Kimura-Sakiyama & H. Tobita
    • Journal Title

      J. Mol. Biol

      Volume: 414 Pages: 765-782

    • NAID

      120003726650

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interaction sites of tropomyosin in muscle thin filament as identified by site-directed spin-labeling2011

    • Author(s)
      K. Ueda, C. Kimura-Sakiyama, T. Aihara, M. Miki & T. Arata
    • Journal Title

      Biophys. J.

      Volume: 100 Pages: 2432-2439

    • NAID

      120003726649

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interaction Sites of Tropomyosin in Muscle Thin Filament as Identified by Site-Directed Spin-Labeling2011

    • Author(s)
      Ueda, K., Kimura-Sakiyama, C., Aihara, T., Miki, M., Arata, T.
    • Journal Title

      Biophysical Journal

      Volume: 100 Issue: 10 Pages: 2432-2439

    • DOI

      10.1016/j.bpj.2011.03.021

    • NAID

      120003726649

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Three-Dimensional FRET Analysis to Construct an Atomic Model of the Actin-Tropomyosin Complex on a Reconstituted Thin Filament2011

    • Author(s)
      Miki, M., Makimura, S., Saitoh, T., Bunya, M., Sugahara, Y., Ueno, Y., Kimura-Sakiyama, C., Tobita, H.
    • Journal Title

      Journal of Molecular Biology

      Volume: 414 Issue: 5 Pages: 765-782

    • DOI

      10.1016/j.jmb.2011.10.033

    • NAID

      120003726650

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Switch action of Troponin on Muscle Thin Filament as Revealed by Spin Labeling and Pulsed EPR2010

    • Author(s)
      T. Aihara, M. Nakamura, S. Ueki, H. Hara, M. Miki, and T. Arata
    • Journal Title

      J. Biol. Chem

      Volume: 285 Pages: 10671-10677

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Switch Action of Troponin on Muscle Thin Filament as Revealed by Spin Labeling and Pulsed ESR2010

    • Author(s)
      Aihara, Nakamura, Ueki, Hara, Mild, Arata
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 1285 Pages: 10671-1067

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] A Three-Dimensional FRET Analysis to Construct an Atomic Model of the Actin. Tropomyosin. Troponin Core Domain Complex on a Muscle Thin Filament2012

    • Author(s)
      Masao Miki
    • Organizer
      International Workshop "from Structure to Dynamics : for Our Understanding of Protein-Protein Interactions"
    • Place of Presentation
      長浜
    • Year and Date
      2012-03-18
    • Related Report
      2011 Final Research Report
  • [Presentation] A Three-Dimensional FRET Analysis to Locate the Troponin Core Domain on a Reconstituted Thin Filament2012

    • Author(s)
      三木正雄、牧村敏史、菅原康之、山田隆太、文屋正志、飛田英孝
    • Organizer
      生体運動研究合同班会議
    • Place of Presentation
      筑波大学
    • Year and Date
      2012-01-06
    • Related Report
      2011 Final Research Report
  • [Presentation] A Three-Dimensional FRET Analysis to Locate the Troponin Core Domain on the Reconstituted Thin Filament2012

    • Author(s)
      三木正雄、牧村敏史、菅原康之、山田隆太、文屋正志、斎藤隆弘、飛田英孝
    • Organizer
      生体運動合同研究班会議
    • Place of Presentation
      筑波大学
    • Year and Date
      2012-01-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] 三次元FRET解析によるFアクチンートロポニンコアドメイン複合体のアトミックモデル構築2011

    • Author(s)
      三木正雄、牧村敏史、菅原康之、山田隆太、文屋正志、飛田英孝
    • Organizer
      日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学姫路
    • Year and Date
      2011-09-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] 三次元FRET解析によるFアクチンートロポニンコアドメイン複合体のアトミックモデル構築2011

    • Author(s)
      三木正雄、牧村敏史、菅原康之、山田隆太、文屋正志、飛田英孝
    • Organizer
      日本生物物理学会第49回年会
    • Place of Presentation
      兵庫県立大学姫路
    • Year and Date
      2011-09-08
    • Related Report
      2011 Final Research Report
  • [Presentation] 三次元FRET解析によるFアクチンートロポミオシン複合体のアトミックモデル構築2011

    • Author(s)
      三木正雄,牧村敏史,文屋正志,斉藤隆弘,菅原康之,飛田英孝
    • Organizer
      生体運動研究合同班会議
    • Place of Presentation
      大阪市大
    • Year and Date
      2011-01-07
    • Related Report
      2011 Final Research Report
  • [Presentation] 三次元FRET解析によるFアクチン-トロポミオシン複合体のアトミックモデル構築2011

    • Author(s)
      三木正雄, 牧村敏史, 文屋正志, 斉藤隆弘, 菅原康之, 飛田英孝
    • Organizer
      生体運動研究合同班会議
    • Place of Presentation
      大阪市立大学(大阪府)
    • Year and Date
      2011-01-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] FRET between probes on Actin and Tropomyosin in the Reconstituted Thin Filament for Constructing an Atomic Model of the FA-Tm Complex2010

    • Author(s)
      牧村敏史、文屋正志、斉藤隆弘、菅原康之、飛田英孝、三木正雄
    • Organizer
      日本生物物理学会第48回年会
    • Place of Presentation
      東北大学
    • Year and Date
      2010-09-20
    • Related Report
      2011 Final Research Report
  • [Presentation] FRET between probes on Actin and Tropomyosin in the Reconstituted Thin Fila ment for Constructing an Atomic Model of the FA-Tm Complex2010

    • Author(s)
      牧村敏史、文屋正志、斉藤隆弘、菅原康之、飛田英孝、三木正雄
    • Organizer
      日本生物理学会年会
    • Place of Presentation
      東北大学(宮城県)
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] Three-Dimensional FRET Analysis for Constructing an Atomic Model of the Muscle Thin Filament2010

    • Author(s)
      三木正雄、飛田英孝、斎藤隆広、牧村敏史、文屋正志
    • Organizer
      生体運動研究合同班会議
    • Place of Presentation
      東京大学
    • Year and Date
      2010-01-10
    • Related Report
      2011 Final Research Report
  • [Presentation] Three-Dimensional FRET Analysis for Constructing an Atomic Model of the Muscle Thin Filament2010

    • Author(s)
      三木正雄, 飛田英孝, 斎藤隆広, 牧村敏史, 文屋正志
    • Organizer
      生体運動研究合同班会議
    • Place of Presentation
      生体運動研究合同班会議
    • Year and Date
      2010-01-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] FRET between Residues on Actin and the N-terminal region(83-111) of Tropomyosin in the Reconstituted Thin Filament2009

    • Author(s)
      M. Bunya, S. Makimura, H. Tobita, and M. Miki
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      徳島文理大学
    • Year and Date
      2009-11-01
    • Related Report
      2011 Final Research Report
  • [Presentation] FRET between Residues on Actin and the N-terminal region (83-111) of Tropomyosin in the Reconstituted Thin Filament2009

    • Author(s)
      M.Bunya, S.Makimura, H.Tobita, M.Miki
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      日本生物物理学会第47回年会
    • Year and Date
      2009-11-01
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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