Development of Analytical System of Structure and Function of Membrane Proteins with Surface-enhanced Infrared Spectroscopy

• Project/Area Number: 21570165
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Gunma University
• Principal Investigator: SONOYAMA Masashi 群馬大学, 大学院・工学研究科, 教授 (40242242)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 膜タンパク質 / 赤外分光 / 構造・機能解析 / 可溶化 / 界面活性剤 / リン脂質 / 膜タンパク / 再構成 / 可溶 / 金薄膜 / 表面プラズモン共鳴 / 構造安定性 / 金簿膜 / 表面

Research Abstract

Experimental techniques on membrane proteins for solubilizing with short-chain phospholipids, reconstituting into partially fluorinated phospholipid vesicles and on a sensitive infrared detection with surface-enhanced effects have been developed for constructing an analytical system of structure and function of membrane proteins. It has been demonstrated that short-chain phospholipids are powerful for solubilization of membrane proteins and that vesicles of partially fluorinated phospholipids are suitable materials for incorporating membrane proteins under native conditions. An analytical system with surface-enhanced infrared spectroscopy has been developed for membrane proteins research.

Research Products

• [Journal Article] Solubilizaiotn and structuralstability of bacteriorhodopsin with amild nonionic detergent n-octyl-β-thioglucoside (2011)
  • Author(s): A. Asada and M. Sonoyama
  • Journal Title: Biosci. Biotech. Biochem Volume: 75 Pages: 376-378
  • Peer Reviewed
• [Journal Article] Solubilizaiotn and structural stability of bacteriorhodopsin with a mild nonionic detergent n-octyl-β-thioglucoside (2011)
  • Author(s): Asada, A., Sonoyama, M.
  • Journal Title: Biosci.Biotech.Biochem. Volume: 7 Pages: 376-378
  • Peer Reviewed
• [Journal Article] Effect of lipid phasetransition on molecular assembly andstructural stability of bacteriorhodopsin reconstituted into phosphatidylcholine liposomes with different acyl-chain lengths (2010)
  • Author(s): Y. Yokoyama, L. Negishi, T. Kitoh, M. Sonoyama, Y. Asami, S. Mitaku
  • Journal Title: J. Phys.Chem. B Volume: 114 Pages: 15706-15711
  • Peer Reviewed
• [Journal Article] Highly stable solubilization of membrane protein bacteriorhodopsin with a short chain phospholipid diheptanoylphosphatidylcholine (2010)
  • Author(s): M. Sonoyama, M. Fukumoto, Y. Kuwabara
  • Journal Title: Chem. Lett Volume: 39 Pages: 876-877
  • NAID: 10027184098
  • Peer Reviewed
• [Journal Article] Structural changes of bacteriorhodopsin in purplemembranes induced by irreversible photobleaching with heterogeneous and homogeneous stability (2010)
  • Author(s): Y. Yokoyama, M. Sonoyama, S. Mitaku
  • Journal Title: Photochem. Photobiol Volume: 86 Pages: 297-301
  • Peer Reviewed
• [Journal Article] Structural changes of bacteriorhodopsin in purple membranes induced by irreversible photobleaching with heterogeneous and homogeneous stability (2010)
  • Author(s): Yokoyama, Y., Sonoyama, M., Mitaku, S.
  • Journal Title: Photochem. Photobiol. Volume: 86 Pages: 297-301
  • Peer Reviewed
• [Journal Article] Highly stable solubilization of membrane protein bacteriorhodopsin with a short chain phospholipid diheptanoylphosphatidylcholine (2010)
  • Author(s): Sonoyama, M., Fukumoto, M., Kuwabara, Y.
  • Journal Title: Chem.Lett. Volume: 39 Pages: 876-877
  • NAID: 10027184098
  • Peer Reviewed
• [Journal Article] Effect of lipid phase transition on molecular assembly and structural stability of bacteriorhodopsin reconstituted into phosphatidylcholine liposomes with different acyl-chain lengths (2010)
  • Author(s): Yokoyama, Y., Negishi, L., Kitoh, T., Sonoyama, M., Asami, Y., Mitaku, S.
  • Journal Title: J.Phys.Chem. Volume: 114 Pages: 15706-15711
  • Peer Reviewed
• [Journal Article] Structural changes of bacteriorhodopsin in purple membranes induced by irreversible photobleaching with heterogeneous and homogeneous stability (2010)
  • Author(s): Yokoyama, Y., Sonoyama, M., Mitaku, S.
  • Journal Title: Photochem.Photobiol. 86 Pages: 297-301
  • Peer Reviewed
• [Journal Article] Effect of molecular assemblyon photocycle of reconstituted bacteriorhodops in : Significant blueshift of the late M photointermediatein the liquid crystalline phase (2009)
  • Author(s): M. Sonoyama, T. Kikukawa, Y. Yokoyama, M. Demura, N. Kamo, S. Mitaku
  • Journal Title: Chem.Lett Volume: 38 Pages: 1134-1135
  • Peer Reviewed
• [Journal Article] Effect of molecular assembly on photocycle of reconstituted bacteriorhodopsin : Significant blue shift of the late M photointermediate in the liquid crystalline phase (2009)
  • Author(s): Sonoyama, M., Kikukawa, T., Yokoyama, Y., Demura, M., Kamo, N., Mitaku, S.
  • Journal Title: Chem.Lett. 38 Pages: 1134-1135
  • NAID: 10027175463
  • Peer Reviewed
• [Journal Article] Bisphenol A weakens calcium binding affinity of sites III and IV in C-terminal domain of bovine brain calmodulin (2009)
  • Author(s): Murayama, K., Sonoyama, M., Terada, T., Yokoyama, Y., Nara, M., Tomida, M., Matsuda, S.
  • Journal Title: Chem.Lett. 38 Pages: 1062-1063
  • NAID: 10025548025
  • Peer Reviewed
• [Presentation] 新規部分フッ素化燐脂質の膜タンパク質研究への応用 (2012)
  • Author(s): 園山正史, 吉野賢, 高木俊之, 高橋浩, 馬場照彦, 金森敏幸
  • Organizer: 第11回産総研・産技連LS-BT合同研究発表会
  • Place of Presentation: 産業総合技術研究所つくばセンター共用講堂(茨城県)
  • Year and Date: 2012-01-31
• [Presentation] Structure and Function of Bacteriorhodopsin Reconstituted into Partially Fluorinated Phosphatidylcholine Liposome (2012)
  • Author(s): M. Yoshino, T. Kikukawa, T. Takagi, Y. Yokoyama, H. Takahashi, T. Baba, T. Kanamori, M. Demura, M. Sonoyama
  • Organizer: 5th International Symposium on MolecularScience of Fluctuations toward Biological Functions
  • Place of Presentation: 東大寺総合文化センター(奈良県)
  • Year and Date: 2012-01-07
• [Presentation] Structure and Function of Bacteriorhodopsin Reconstituted into Partially Fluorinated Phosphatidylcholine Liposome (2012)
  • Author(s): M.Yoshino, T.Kikukawa, T.Takagi, Y.Yokoyama, H.Takahashi, T.Baba, T.Kanamori, M.Demura, M.Sonoyama
  • Organizer: 5th International Symposium on Molecular Science of Fluctuations toward Biological Functions
  • Place of Presentation: 東大寺総合文化センター(奈良県)
  • Year and Date: 2012-01-07
• [Presentation] A Comparative Study of Reconstituted Bacteriorhodopsin in Partially Fluorinated and Unfluorinated Phosphatidylcholine Liposome (2011)
  • Author(s): M. Yoshino, T. Kikukawa, T. Takagi, Y.Yokoyama, H. Takahashi, M. Demura, T.Baba, T. Kanamori, M. Sonoyama
  • Organizer: 1st International Conference on ElementInnovation
  • Place of Presentation: 桐生市民会館(群馬県)
  • Year and Date: 2011-12-09
• [Presentation] A Comparative Study of Reconstituted Bacteriorhodopsin in Partially Fluorinated and Unfluorinated Phosphatidylcholine Liposome (2011)
  • Author(s): M.Yoshino, T.Kikukawa, T.Takagi, Y.Yokoyama, H.Takahashi, M.Demura, T.Baba, T.Kanamori, M.Sonoyama
  • Organizer: 1st International Conference on Element Innovation
  • Place of Presentation: 桐生市民会館(群馬県)
  • Year and Date: 2011-12-09
• [Presentation] Decay Kinetics of the M Photointermediate of Bacteriorhodopsin in Short Chain Phosphatidylcholine Micelles (2011)
  • Author(s): Y. Kuwabara, M. Fukumoto, T. Kikukawa,M. Demura, M. Sonoyama
  • Organizer: 日本生物物理学会第49回年会
  • Place of Presentation: 兵庫県立大学書写キャンパス(兵庫県)
  • Year and Date: 2011-09-16
• [Presentation] Reconstitution of Bacteriorhodopsininto Partially Fluorinated Phosphatidylcholine Liposomes (2011)
  • Author(s): M. Yoshino, T. Kikukawa, Y. Yokoyama T. Takagi, H. Takahashi, M. Demura, T.Baba, T. Kanamori, M. Sonoyama
  • Organizer: 日本生物物理学会第49回年会
  • Place of Presentation: 兵庫県立大学書写キャンパス(兵庫県)
  • Year and Date: 2011-09-16
• [Presentation] Reconstitution of Bacteriorhodopsin into Partially Fluorinated Phosphatidylcholine Liposomes (2011)
  • Author(s): M.Yoshino, T.Kikukawa, Y.Yokoyama, T.Takagi, H.Takahashi, M.Demura, T.Baba, T.Kanamori, M.Sonoyama
  • Organizer: 日本生物物理学会第49回年会
  • Place of Presentation: 兵庫県立大学書写キャンパス(兵庫県)
  • Year and Date: 2011-09-16
• [Presentation] 新規部分フッ素化リン脂質1,2-Di(14,14,14,13,13,12,12,11,11-nonafluorotetradecanoil)-glycero-3-phosphorylcholine二重膜の熱物性と膜の安定性 (2011)
  • Author(s): 上治瑛, 吉野賢, 高橋浩, 高木俊之, 馬場照彦, 金森敏幸, 園山正史
  • Organizer: 第5回バイオ関連化学シンポジウム
  • Place of Presentation: つくば国際会議場(茨城県)
  • Year and Date: 2011-09-13
• [Presentation] Highly stablesolubilization of membrane proteinswith a short chain phospholipid (2010)
  • Author(s): M. Sonoyama, M. Fukumoto, Y.Kuwabara
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2010-12-09
• [Presentation] Highly stable solubilization of membrane proteins with a short chain phospholipid (2010)
  • Author(s): M.Sonoyama, M.Fukumoto, Y.Kuwabara
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-12-09
• [Presentation] 短鎖リン脂質ジヘプタノイルフォスファチジルコリンで可溶化したバクテリオロドプシンの構造と光機能中間体 (2010)
  • Author(s): 桑原由美子・福元美奈・菊川峰志・出村誠・園山正史
  • Organizer: 日本化学会関東支部群馬地区懇談会
  • Place of Presentation: 日本原子力研究開発機構高崎量子応用研究所
  • Year and Date: 2010-11-22
• [Presentation] 新規部分フッ素化フォスファチジルコリン小胞への膜タンパク質バクテリオロドプシンの再構成 (2010)
  • Author(s): 吉野賢・菊川峰志・横山泰範・高木俊之・高橋浩・出村誠・馬場照彦・金森敏幸・園山正史
  • Organizer: 日本化学会関東支部群馬地区懇談会
  • Place of Presentation: 日本原子力研究開発機構高崎量子応用研究所
  • Year and Date: 2010-11-22
• [Presentation] 新規合成部分フッ素化フォスファチジルコリン二重層膜の構造・物性評価 (2010)
  • Author(s): 高橋浩・吉野賢・高木俊之・馬場照彦・金森敏幸・園山正史
  • Organizer: 日本化学会関東支部群馬地区懇談会
  • Place of Presentation: 日本原子力研究開発機構高崎量子応用研究所
  • Year and Date: 2010-11-22
• [Presentation] 日本生物物理学会第48回年会 (2010)
  • Author(s): M. Yoshino, H. Takahashi, T. Takagi,T. Baba, T. Kanamori, M. Sonoyama
  • Organizer: Structure and thermal properties of binary lipid bilayers of partially fluorinated and normal phosphatidylcholine
  • Place of Presentation: 東北大学川内北キャンパス(宮城県)
  • Year and Date: 2010-09-22
• [Presentation] Effect of molecular assembly upon structure and structural stability of bacteriorhodopsin reconstituted into phosphatidylcholine lipid liposomes (2010)
  • Author(s): T. Kito, Y. Yokoyama, R. Negishi, M. Sonoyama, S. Mitaku
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学川内北キャンパス(宮城県)
  • Year and Date: 2010-09-22
• [Presentation] Dynamic study of bacteriorhodopsin-Triton X-100 complex in solution by dynamic hght Scattering (2010)
  • Author(s): A.Yanagawa, I.Tsukushi, M.Sonoyama
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東邦大学川内北キャンパス
  • Year and Date: 2010-09-22
• [Presentation] Effect of molecular assembly upon structure and structural stability of bacteriorhodopsin reconstituted into phosphatidylchohne lipid liposomes (2010)
  • Author(s): T.Kito, Y.Yokoyama, R.Negishi, M.Sonoyama, S.Mitaku
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東邦大学川内北キャンパス
  • Year and Date: 2010-09-22
• [Presentation] Structure and thermal properties of binary lipid bilayers of partially fluorinated and normal phosphatidylcholine (2010)
  • Author(s): M.Yoshino, H.Takahashi, T.Takagi, T.Baba, T.Kanamori, M.Sonoyama
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東邦大学川内北キャンパス
  • Year and Date: 2010-09-22
• [Presentation] Structuralstability and photocycle of bacteriorhodopsin in short chain phospholipid micelles (2010)
  • Author(s): M. Fukumoto, Y. Kuwabara, T. Kikukawa, M. Demura, M. Sonoyama
  • Organizer: 日本生物物理学会第48回年会、
  • Place of Presentation: 東北大学川内北キャンパス(宮城県)
  • Year and Date: 2010-09-20
• [Presentation] Structural stability and photocycle of bacteriorhodopsin in short chain phospholipid micelles (2010)
  • Author(s): M.Fukumoto, Y.Kuwabara, T.Kikukawa, M.Demura, M.Sonoyama
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東邦大学川内北キャンパス
  • Year and Date: 2010-09-20
• [Presentation] アシル鎖長の異なる3種類の脂質膜リポソームに再構成されたバクテリオロドプシンにおける分子集合状態ならびに構造安定性に対する脂質膜相転移の影響 (2010)
  • Author(s): 鬼頭琢, 横山泰範, 根岸瑠美, 麻見安雄, 園山正史, 美宅成樹
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2010-06-17
• [Presentation] 短鎖リン脂質ジヘプタノイルホスファチジルコリンによる膜タンパク質バクテリオロドプシンの可溶化 (2010)
  • Author(s): 福元美奈, 桑原由美子, 園山正史
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2010-06-17
• [Presentation] 短鎖リン脂質ジヘプタノイルホスファチジルコリンによる膜タンパク質バクテリオロドプシンの可溶化 (2010)
  • Author(s): 福元美奈・桑原由美子・園山正史
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2010-06-17
• [Presentation] アシル鎖長の異なる3種類の脂質膜リポソームに再構成されたバクテリオロドプシンにおける分子集合状態ならびに構造安定性に対する脂質膜相転移の影響 (2010)
  • Author(s): 鬼頭琢・横山泰範・根岸瑠美・麻見安雄・園山正史・美宅成樹
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2010-06-17
• [Presentation] Photobleaching of bacteriorhodopsin reconstituted in DMPC and DPP Cbilayer (2009)
  • Author(s): T. Kitoh, L. Negishi, Y. Yokoyama, M.Sonoyama, S. Mitaku
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: アスティー徳島(徳島県)
  • Year and Date: 2009-11-01
• [Presentation] Identification of cytoplasmic and extracellular side of bacteriorhodopsin for force spectroscopy (2009)
  • Author(s): 東陽介, 尾崎聡, 渡邉直哉, 横山泰範, 園山正史, 美宅成樹
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: アスティー徳島(徳島県)
  • Year and Date: 2009-11-01
• [Presentation] Effect of protein-lipid interaction on irreversible photobleaching of bacteriorhodopsin in purple membrane (2009)
  • Author(s): 成重太助, 横山泰範, 園山正史, 美宅成樹
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: アスティー徳島(徳島県)
  • Year and Date: 2009-11-01
• [Presentation] Photobleaching of bacteriorhodospin reconstituted in DMPC and DPPC bilayer (2009)
  • Author(s): 鬼頭琢, 根岸瑠美, 横山泰範, 園山正史, 美宅成樹
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: アスティー徳島(徳島県)
  • Year and Date: 2009-11-01
• [Presentation] Circular dichroism measurements of bacteriorhodopsin reconstituted in DMPC and DPPC bilayer in visible region (2009)
  • Author(s): 根岸瑠美, 鬼頭琢, 横山泰範, 園山正史, 美宅成樹
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: アスティー徳島(徳島県)
  • Year and Date: 2009-10-30
• [Presentation] Bisphenol A weakens calcium binding affinity of calmodulin (2009)
  • Author(s): Murayama, K., 園山正史, Terada, T., 横山泰範, Nara, M., Tomida, M., Matsuda, S.
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: アスティー徳島(徳島県)
  • Year and Date: 2009-10-30
• [Presentation] バクテリオロドプシンのアルカリ化に伴う二次元結晶構造の崩壊過程 (2009)
  • Author(s): 東陽介・尾崎聡・渡邉直哉・横山泰範・園山正史・美宅成樹
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 熊本全日空ホテルニュースカイ(熊本県)
  • Year and Date: 2009-05-23
• [Presentation] 脂質二重膜に再構成されたバクテリオロドプシンの脂質膜相転移への影響 (2009)
  • Author(s): 鬼頭琢, 根岸瑠美, 横山泰範, 園山正史, 美宅成樹
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 熊本全日空ホテルニュースカイ(熊本県)
  • Year and Date: 2009-05-22
• [Presentation] 紫膜バクテリオロドプシンの光退色現象におけるタンパク質-脂質相互作用の影響 (2009)
  • Author(s): 成重太助・横山泰範・園山正史・美宅成樹
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 熊本全日空ホテルニュースカイ(熊本県)
  • Year and Date: 2009-05-21
• [Presentation] 短鎖リン脂質により可溶化したバクテリオロドプシンの構造安定性 (2009)
  • Author(s): 園山正史, 福元美奈, 桑原由美子, 菊川峰志, 出村誠
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 近畿大学(大阪府)
  • Year and Date: 2009-03-26
• [Remarks]
  • URL: http://www.chem-bio.gunma-u.ac.jp/~biomolsci/public.html