Development of Analytical System of Structure and Function of Membrane Proteins with Surface-enhanced Infrared Spectroscopy
Project/Area Number |
21570165
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biophysics
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Research Institution | Gunma University |
Principal Investigator |
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Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 膜タンパク質 / 赤外分光 / 構造・機能解析 / 可溶化 / 界面活性剤 / リン脂質 / 膜タンパク / 再構成 / 可溶 / 金薄膜 / 表面プラズモン共鳴 / 構造安定性 / 金簿膜 / 表面 |
Research Abstract |
Experimental techniques on membrane proteins for solubilizing with short-chain phospholipids, reconstituting into partially fluorinated phospholipid vesicles and on a sensitive infrared detection with surface-enhanced effects have been developed for constructing an analytical system of structure and function of membrane proteins. It has been demonstrated that short-chain phospholipids are powerful for solubilization of membrane proteins and that vesicles of partially fluorinated phospholipids are suitable materials for incorporating membrane proteins under native conditions. An analytical system with surface-enhanced infrared spectroscopy has been developed for membrane proteins research.
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Report
(4 results)
Research Products
(48 results)
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[Journal Article] Effect of lipid phase transition on molecular assembly and structural stability of bacteriorhodopsin reconstituted into phosphatidylcholine liposomes with different acyl-chain lengths2010
Author(s)
Yokoyama, Y., Negishi, L., Kitoh, T., Sonoyama, M., Asami, Y., Mitaku, S.
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Journal Title
J.Phys.Chem.
Volume: 114
Pages: 15706-15711
Related Report
Peer Reviewed
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[Journal Article] Effect of molecular assembly on photocycle of reconstituted bacteriorhodopsin : Significant blue shift of the late M photointermediate in the liquid crystalline phase2009
Author(s)
Sonoyama, M., Kikukawa, T., Yokoyama, Y., Demura, M., Kamo, N., Mitaku, S.
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Journal Title
Chem.Lett. 38
Pages: 1134-1135
NAID
Related Report
Peer Reviewed
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[Journal Article] Bisphenol A weakens calcium binding affinity of sites III and IV in C-terminal domain of bovine brain calmodulin2009
Author(s)
Murayama, K., Sonoyama, M., Terada, T., Yokoyama, Y., Nara, M., Tomida, M., Matsuda, S.
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Journal Title
Chem.Lett. 38
Pages: 1062-1063
NAID
Related Report
Peer Reviewed
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[Presentation] 新規部分フッ素化リン脂質1,2-Di(14,14,14,13,13,12,12,11,11-nonafluorotetradecanoil)-glycero-3-phosphorylcholine二重膜の熱物性と膜の安定性2011
Author(s)
上治瑛, 吉野賢, 高橋浩, 高木俊之, 馬場照彦, 金森敏幸, 園山正史
Organizer
第5回バイオ関連化学シンポジウム
Place of Presentation
つくば国際会議場(茨城県)
Year and Date
2011-09-13
Related Report
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[Presentation] 日本生物物理学会第48回年会2010
Author(s)
M. Yoshino, H. Takahashi, T. Takagi,T. Baba, T. Kanamori, M. Sonoyama
Organizer
Structure and thermal properties of binary lipid bilayers of partially fluorinated and normal phosphatidylcholine
Place of Presentation
東北大学川内北キャンパス(宮城県)
Year and Date
2010-09-22
Related Report
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[Presentation] Bisphenol A weakens calcium binding affinity of calmodulin2009
Author(s)
Murayama, K., 園山正史, Terada, T., 横山泰範, Nara, M., Tomida, M., Matsuda, S.
Organizer
日本生物物理学会第47回年会
Place of Presentation
アスティー徳島(徳島県)
Year and Date
2009-10-30
Related Report
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