Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Research Abstract |
The novel molecular biological system, which artificially alter the homodimeric structure of the photosynthetic reaction center(RC) to the heterodimeric one, has been constructed in the green sulfur bacterium, Chlorobaculum tepidum. By the tandem chromatographic procedures using Ni-NTA and Strep-tactin resins, the Strep-PscA/ His-PscA-type RC could be obtained successfully. The method for the transformation system with a broad-host-range plasmid has also been developed to simplify genetic analyses, and will be a useful tool in the near future.
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