| Project/Area Number |
21570171
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | University of Hyogo |
|---|
Principal Investigator |
OGURA Takashi 兵庫県立大学, 大学院・生命理学研究科, 教授 (70183770)
|
|---|
| Research Collaborator |
YANAGISAWA Sachiko 兵庫県立大学, 大学院・生命理学研究科, 助教 (60557982)
NAKASHIMA Satoru 兵庫県立大学, 大学院・生命理学研究科, 特任准教授 (80263234)
KUBO Minoru 兵庫県立大学, 大学院・生命理学研究科, 特任助教 (90392878)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | 好気呼吸 / 生体エネルギー変換 / チトクロムC酸化酵素 / 酸素還元 / プロトンポンプ / 共鳴ラマン / 部位特異的アミノ酸置換 / 振動スペクトル / ヘム / 水素結合 / ミトコンドリア / 細胞呼吸 / チトクロムc酸化酵素 / 酸素活性化 / タンパク質構造ダイナミクス / 酸化的リン酸化 / 呼吸鎖電子伝達系 / 構造ダイナミクス / 鉄-ヒスチジン結合 |
|---|
| Research Abstract |
Cytochrome c oxidase plays an essentially important function in cell respiration to maintain life, and catalyzes dioxygen reduction to water, which is coupled with proton pumping across the membrane. In order to elucidate the mechanism of this enzyme, high resolution structures at a picometer level are to be determined by resonance Raman spectroscopy. In the present study, we have established assignments of some Raman siganals. In addition, an intermediate conformational state and cooperative dynamics of the two hemes have been detected, which are essential for an efficient proton pump.
|
