Resonance Raman Study on the Proton Pumping Mechanism of Cytochrome c Oxidase

• Project/Area Number: 21570171
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: University of Hyogo
• Principal Investigator: OGURA Takashi 兵庫県立大学, 大学院・生命理学研究科, 教授 (70183770)
• Research Collaborator: YANAGISAWA Sachiko 兵庫県立大学, 大学院・生命理学研究科, 助教 (60557982) ; NAKASHIMA Satoru 兵庫県立大学, 大学院・生命理学研究科, 特任准教授 (80263234) ; KUBO Minoru 兵庫県立大学, 大学院・生命理学研究科, 特任助教 (90392878)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 好気呼吸 / 生体エネルギー変換 / チトクロムC酸化酵素 / 酸素還元 / プロトンポンプ / 共鳴ラマン / 部位特異的アミノ酸置換 / 振動スペクトル / ヘム / 水素結合 / ミトコンドリア / 細胞呼吸 / チトクロムc酸化酵素 / 酸素活性化 / タンパク質構造ダイナミクス / 酸化的リン酸化 / 呼吸鎖電子伝達系 / 構造ダイナミクス / 鉄-ヒスチジン結合

Research Abstract

Cytochrome c oxidase plays an essentially important function in cell respiration to maintain life, and catalyzes dioxygen reduction to water, which is coupled with proton pumping across the membrane. In order to elucidate the mechanism of this enzyme, high resolution structures at a picometer level are to be determined by resonance Raman spectroscopy. In the present study, we have established assignments of some Raman siganals. In addition, an intermediate conformational state and cooperative dynamics of the two hemes have been detected, which are essential for an efficient proton pump.

Research Products

• [Journal Article] An intermediate conformational state during ligand binding to cytochrome c oxidase detected by time-resolved resonance Raman analyses of heme peripheral groups (2012)
  • Author(s): Izumi Ishigami, Takeshi Nishigaki, Kyoko Shinzawa-Itoh, Shinya Yoshikawa, Satoru Nakashima and Takashi Ogura
  • Journal Title: Chem. Lett. Volume: 2 Issue: 2 Pages: 178-180
  • DOI: 10.1246/cl.2012.178
  • NAID: 130004426495
  • Peer Reviewed
• [Journal Article] T-quaternary structure of oxy human adult hemoglobin in the presence of two allosteric effectors, L35 and IHP (2011)
  • Author(s): Kenji Kanaori, Yusuke Tajiri, Antonio Tsuneshige, Izumi Ishigami, Takashi Ogura, Kunihiko Tajima, Saburo Neya and Takashi Yonetani
  • Journal Title: Biochim. Biophys. Acta-Bioenergetics Volume: 1807 Issue: 10 Pages: 1253-1261
  • DOI: 10.1016/j.bbabio.2011.06.004
  • Peer Reviewed
• [Journal Article] Post-Translational His-Cys Cross-Linkage Formation in Tyrosinase Induced by Copper(II)-Peroxo Species (2011)
  • Author(s): Nobutaka Fujieda, Takuya Ikeda, Michiaki Murata, Sachiko Yanagisawa, Shigetoshi Aono, Kei Ohkubo, Satoshi Nagao, Takashi Ogura, Shun Hirota, Shunichi Fukuzumi, Yukihiro Nakamura, Yoji Hata, Shinobu Itoh
  • Journal Title: Journal of the American Chemical Society Volume: 133 Issue: 5 Pages: 1180-1183
  • DOI: 10.1021/ja108280w
  • Peer Reviewed
• [Journal Article] Resonance Raman study on the oxygenated and the ferryl-oxo species of indoleamine 2, 3-dioxygenase during catalytic turnover (2011)
  • Author(s): Sachiko Yanagisawa, Masaki Horitani, Hiroshi Sugimoto, Yoshitsugu Shiro, Norihiro Okada, and Takashi Ogura
  • Journal Title: Faraday Disc Volume: 148 Pages: 239-247
  • DOI: 10.1039/c004552g
  • Peer Reviewed
• [Journal Article] Spectroscopic and computational characterization of Cu^<II>-OOR(R=H or cumyl) complexes bearing a Me_6-tren ligand (2011)
  • Author(s): Yu Jin Choi, et al.
  • Journal Title: Dalton Trans. Volume: 40 Pages: 2234-2241
  • Peer Reviewed
• [Journal Article] Post-translational His-Cys Cross Linkage Formation in Tyrosinase Induced by Copper(II)-Peroxo Species (2011)
  • Author(s): Nobutaka Fujieda, et al.
  • Journal Title: J.Am.Chew.Soc. Volume: 133 Pages: 1180-1183
  • Peer Reviewed
• [Journal Article] Resonance Raman study on the oxygenated and the ferryl-oxo species of indoleamine 2,3-dioxygenase during catalytic turnover (2011)
  • Author(s): Sachiko Yanagisawa, et al.
  • Journal Title: Faraday Disc. Volume: 148 Pages: 239-247
  • Peer Reviewed
• [Journal Article] Important Roles of Tyr43 at the Putative Heme Distal Side in Oxygen Recognition and Stability of the Fe(II)-O_2 Complex of YddV, a Globin-coupled Heme-based Oxygen Sensor Diguanylate Cyclase (2010)
  • Author(s): Kenichi Kitanishi, et al.
  • Journal Title: Biochemistry Volume: 49 Pages: 10381-10393
  • Peer Reviewed
• [Journal Article] A Specific Interaction of L-Tryptophan with CO of CO-Bound Indoleamine 2,3-Dioxygenase Identified by Resonance Raman Spectroscopy (2010)
  • Author(s): Sachiko Yanagisawa, et al.
  • Journal Title: Biochemistry Volume: 49 Pages: 10081-10088
  • Peer Reviewed
• [Journal Article] Synthesis, Structural and Spectroscopic Characterization, and Reactivities of Mononuclear Cobalt(III)-Peroxo Complexes (2010)
  • Author(s): Jaeheung Cho, et al.
  • Journal Title: J.Am.Chem.Soc. Volume: 132 Pages: 16977-16986
  • Peer Reviewed
• [Journal Article] A Low-Spin Ruthenium(IV)-Oxido Complex : Does the Spin State Have an Impact on the Reactivity? (2010)
  • Author(s): Takahiko Kojima, et al.
  • Journal Title: Angew.Chem.Int.Ed. Volume: 49 Pages: 8449-8453
  • Peer Reviewed
• [Journal Article] Reversible O-O Bond Cleavage and Formation between Mn(IV)-Peroxo and Mn(V)-Oxo Corroles (2010)
  • Author(s): Sun Hee Kim, et al.
  • Journal Title: J.Am.Chem.Soc. Volume: 132 Pages: 14030-14032
  • Peer Reviewed
• [Journal Article] Cell-free synthesis of cytochrome c oxidase, a multicomponent membrane protein (2010)
  • Author(s): Yukie Katayama, et al.
  • Journal Title: J.Bioener.and Biomemb. Volume: 42 Pages: 235-240
  • Peer Reviewed
• [Journal Article] Nickel(II) Complexes of tpa Ligands with 6-Phenyl Substituents(Ph_ntpa).Structure and H_2O_2-Reactivity (2010)
  • Author(s): T.Tano, et al.
  • Journal Title: Bull.Chem.Soc.Jpn. Volume: 83 Pages: 530-538
  • Peer Reviewed
• [Journal Article] A square-planar Ni(II) complex with an N_2S_2 donor set similar to the active centre of nickel-containing superoxide dismutase and its reaction with superoxide (2010)
  • Author(s): D.Nakane, et al.
  • Journal Title: Chem.Commun. Volume: 46 Pages: 2142-2144
  • Peer Reviewed
• [Journal Article] Resonance Raman study on the oxygenated and the ferryl-oxo species of indoleamine 2, 3-dioxygenase during catalytic turnover (2010)
  • Author(s): Sachiko Yanagisawa, Masaki Horitani, Hiroshi Sugimoto, Yoshitsugu Shiro, Norihiro Okada, Takashi Ogura
  • Journal Title: Faraday Discussions (印刷中)
  • Peer Reviewed
• [Journal Article] Cell-free synthesis of cytochrome c oxidase, a multicomponent membrane protein (2010)
  • Author(s): Yukie Katayama, Kunitoshi Shimokata, Makoto Suematsu, Takashi Ogura, Tomitake Tsukihara, Shinya Yoshikawa, Hideo Shimada
  • Journal Title: J.Bioenergetics and Biomembranes
  • Peer Reviewed
• [Journal Article] A Resonance Raman Band Assignable to the O-O Stretching Mode in the Resting Oxidized State of Bovine Heart Cytochrome c Oxidase (2010)
  • Author(s): Miyuki Sakaguchi, Kyoko Shinzawa-Itoh, Shinya Yoshikawa, Takashi Ogura
  • Journal Title: J.Bioenergetics and Biomembranes
  • Peer Reviewed
• [Journal Article] Nickel(II)complexes of TPA ligands with 6-phenyl substituents PhnTPA. Structure and H_2O_2-reactivity (2010)
  • Author(s): Tano, T., Doi, Y., Inosako, M., Kunishita, A., Kubo, M., Ishimaru, H., Ogura, T., Sugimoto, H., Itoh, S.
  • Journal Title: Bull.Chem.Soc.Jpn. (印刷中)
  • Peer Reviewed
• [Journal Article] A square-planar Ni(II)complex with an N_2S_2 donor set similar to the active centre of nickel-containing superoxide dismutase and its reaction with superoxide (2010)
  • Author(s): Nakane, D., Kuwasako, S., Tsuge, M., Kubo, M., Funahashi, Y., Ozawa, T., Ogura, T., Masuda, H.
  • Journal Title: Chem.Commun. 46 Pages: 2142-2144
  • Peer Reviewed
• [Journal Article] Resonance Raman Study of a High-valent Fe=O Porphyrin Complex as a Model for Peroxide Compound II (2010)
  • Author(s): Hirohito Ishimaru, Hiroshi Fujii, Takashi Ogura
  • Journal Title: Chem.Lett. 39 Pages: 332-333
  • Peer Reviewed
• [Journal Article] Identification of the Fe-O_2 and the Fe=O Heme Species for Indoleamine 2, 3- Dioxygenase during Catalytic Turnover (2010)
  • Author(s): Yanagisawa, S., Yotsuya, K., Hashiwaki, Y., Horitani, M., Sugimoto, H., Shiro, Y., Appelman, E.H., Ogura
  • Journal Title: Chem.Lett. 39 Pages: 36-37
  • NAID: 10027176398
  • Peer Reviewed
• [Journal Article] Spectroscopic Characterization of a Hydroperoxo-Heme Intermediate : Conversion of a Side-On Peroxo to an End-On Hydroperoxo Complex (2009)
  • Author(s): Liu, J.G., Ohta, T., Yamaguchi, S., Ogura, T., Sakamoto, S., Maeda, Y., Naruta, Y.
  • Journal Title: Angew.Chem.Int.Ed. 48 Pages: 9262-9267
  • Peer Reviewed
• [Journal Article] Geometric and electronic structure and reactivity of a mononuclear 'side-on' nickel(III)-peroxo complex (2009)
  • Author(s): Cho, J., Sarangi, R., Annaraj, J., Kim, S.Y., Kubo, M., Ogura, T., Solomon, E.I, Nam, W.
  • Journal Title: Nature Chemistry 1 Pages: 568-572
  • Peer Reviewed
• [Journal Article] Oxidation Reactivity of a Bis(μ-oxo)Dinickel(III)Complexes : Arene Hydroxylation of the Supporting Ligand (2009)
  • Author(s): Honda, K., Cho, J., Matsumoto, T., Roh, J., Furutachi, H., Tosha, T., Kubo, M., Fujinami, S., Ogura, T., Kitagawa, T., Suzuki, M.
  • Journal Title: Angewandte Chemie, Int.Ed. 121 Pages: 3354-3357
  • Peer Reviewed
• [Journal Article] Mononuclear Copper(II)-Superoxo Complexes that Mimic the Structure and Reactivity of the Active Centers of PHM and DβM (2009)
  • Author(s): Kunishita, A., Kubo, M., Sugimoto, H., Ogura, T., Sato, K., Takui, T., Itoh, S.
  • Journal Title: J.Am.Chem.Soc. 131 Pages: 2788-2789
  • Peer Reviewed
• [Presentation] チトクロムc酸化酵素におけるヘムa側鎖ヒドロキシファルネシルエチル基の共鳴ラマン線の帰属 (2012)
  • Author(s): 坂口美幸, その他
  • Organizer: 日本化学会第92春季年会
  • Place of Presentation: 慶応大学、神奈川県
  • Year and Date: 2012-03-27
• [Presentation] フェムト秒レーザーを用いた高感度時間分解赤外分光装置の開発と蛋白質水溶液への応用 (2011)
  • Author(s): 久保稔, その他
  • Organizer: 日本分光学会年次講演会
  • Place of Presentation: 理化学研究所、神奈川県
  • Year and Date: 2011-12-02
• [Presentation] Protein Dy namics of and Reaction Mechanism of Cytochrome c Oxidaseas Studied with Resonance Raman Spectroscop y (2011)
  • Author(s): Izumi Ishigami, et al.
  • Organizer: The 3rd Asian Spectroscopy Conference
  • Place of Presentation: Xiamen University, China
  • Year and Date: 2011-11-30
• [Presentation] Protein Dynamics of and Reaction Mechanism of Cytochrome c Oxidase as Studied with Resonance Raman Spectroscopy (2011)
  • Author(s): Izumi Ishigami, et al
  • Organizer: The 3rd Asian Spectroscopy Conference
  • Place of Presentation: Xiamen University, China
  • Year and Date: 2011-11-30
• [Presentation] Microsecond Protein Dynamics of Cytochrome c Oxidase as Studied with Resonanc e Raman Spectroscopy (2011)
  • Author(s): Takashi Ogura, et al.
  • Organizer: The 3rd Asian Spectroscopy Conference
  • Place of Presentation: Xiamen University, China
  • Year and Date: 2011-11-29
• [Presentation] Microsecond Protein Dynamics of Cytochrome c Oxidase as Studied with Resonance Raman Spectroscopy (2011)
  • Author(s): Takashi Ogura, et al
  • Organizer: The 3rd Asian Spectroscopy Conference
  • Place of Presentation: Xiamen University, China(招待講演)
  • Year and Date: 2011-11-29
• [Presentation] 水溶液中のタンパク質の高感度時間分解赤外分光測定 (2011)
  • Author(s): 久保稔, その他
  • Organizer: 第5回分子科学討論会
  • Place of Presentation: 札幌コンベンションセンター、北海道
  • Year and Date: 2011-09-20
• [Presentation] 水溶液中のタンパク質の高感度時間分解赤外分光測定 (2011)
  • Author(s): 久保稔, 他
  • Organizer: 第5回分子科学討論会
  • Place of Presentation: 札幌コンベンションセンター北海道
  • Year and Date: 2011-09-20
• [Presentation] ヘム鉄の電子密度がミオグロビンの自動酸化反応に与える影響の解明 (2011)
  • Author(s): 西村龍, その他
  • Organizer: 錯体化学会第61回討論会
  • Place of Presentation: 岡山理科大学、岡山県
  • Year and Date: 2011-09-18
• [Presentation] Structural analysis of FMN and iron-sulfur clusters of bovine heart NADH-ubiquinone oxidoreductase by resonance Raman spectroscopy (2011)
  • Author(s): Masahide Hikita, et al
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学、兵庫県
  • Year and Date: 2011-09-18
• [Presentation] Resonance Raman Study on Cytochrome c Oxida se Reconstituted in Phospholipid Vesicles (2011)
  • Author(s): Takashi Nomura, et al.
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学、兵庫県
  • Year and Date: 2011-09-17
• [Presentation] Protein Dy namics and Reaction Mechanism of Cytochrome c Oxidase : Resonance R aman Spectroscopy (2011)
  • Author(s): Izumi Ishigami, et al.
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学、兵庫県
  • Year and Date: 2011-09-17
• [Presentation] Resonance R aman Study on Ligand-Bound Forms of Indoleamine 2, 3-Dioxygenase (2011)
  • Author(s): Masayuki Hara, et al.
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学、兵庫県
  • Year and Date: 2011-09-17
• [Presentation] Resona nce Raman Study on CO-bound Indol eamine 2, 3-Dioxygenase with Tryp tophan (2011)
  • Author(s): Sachiko Yanagisawa, et al.
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学、兵庫県
  • Year and Date: 2011-09-17
• [Presentation] The chem ical nature of the ligand at the oxygen reduction site of the rest ing oxidized cytochrome c oxidase (2011)
  • Author(s): Miyuki Sakaguchi, et al.
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学、兵庫県
  • Year and Date: 2011-09-17
• [Presentation] Protein Dynamics of Cytochrome c Oxidase upon CN-Photolysis as Studied w ith Resonance Raman Spectroscopy (2011)
  • Author(s): Takeshi Nishigaki, et al.
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学、兵庫県
  • Year and Date: 2011-09-17
• [Presentation] Resonan ce Raman study on the ferryl oxo hemes with imidazole as axilal li gand having a bulky substituent (2011)
  • Author(s): Hirohito Ishimaru, et al.
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学、兵庫県
  • Year and Date: 2011-09-17
• [Presentation] Structura l analysis of FMN and iron-sulfur clusters of bovine heart NADH-ub iquinone oxidoreductase by resona nce Raman spectroscopy (2011)
  • Author(s): Masahide Hikita, et al.
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学、兵庫県
  • Year and Date: 2011-09-17
• [Presentation] Resonance Raman Study on Cytochrome c Oxidase Reconstituted in Phospholipid Vesicles (2011)
  • Author(s): Takashi Nomura, et al
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学、兵庫県
  • Year and Date: 2011-09-17
• [Presentation] Protein Dynamics and Reaction Mechan sm of Cytochrome c Oxidase : Resonance Raman Spectroscopy (2011)
  • Author(s): Izumi Ishigami, et al
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学、兵庫県
  • Year and Date: 2011-09-17
• [Presentation] The chemical nature of the ligand at the oxygen reduction site of the resting oxidized cytochrome c oxidase (2011)
  • Author(s): Miyuki Sakaguchi, et al
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学、兵庫県
  • Year and Date: 2011-09-17
• [Presentation] Protein Dynamics of Cytochrome c Oxidase upon CN^- Photolysis as Studied with Resonance Raman Spectroscopy (2011)
  • Author(s): Takeshi Nishigaki, et al
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学、兵庫県
  • Year and Date: 2011-09-17
• [Presentation] Resonance Raman study on the ferryl oxo hemes with imidazole as axilal ligand having a bulky substituent (2011)
  • Author(s): Hirohito Ishimaru, et al
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学、兵庫県
  • Year and Date: 2011-09-17
• [Presentation] Resonance Raman study on Ligand-Bound Forms of Indoleamine 2, 3-Diocygenase (2011)
  • Author(s): Masayuki Hara, et al
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学、兵庫県
  • Year and Date: 2011-09-16
• [Presentation] Resonance Raman Study on CO-bound Indoleamine 2,3-Dioxygenase with Tryptophan (2011)
  • Author(s): Sachiko Yanagisawa, et al
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学、兵庫県
  • Year and Date: 2011-09-16
• [Presentation] 共鳴ラマン分光法によるウシ心筋NADH-ユビキノン酸化還元酵素のFMNとFe-Sクラスターの構造解析 (2011)
  • Author(s): 引田理英, その他
  • Organizer: 第38回生体分子科学討論会
  • Place of Presentation: 筑波大学、茨城県
  • Year and Date: 2011-06-24
• [Presentation] 共鳴ラマン分光法によるインドールアミン2, 3ジオキシゲナーゼの光学異性依存的基質相互作用の検出 (2011)
  • Author(s): 柳澤幸子, その他
  • Organizer: 第38回生体分子科学討論会
  • Place of Presentation: 筑波大学、茨城県
  • Year and Date: 2011-06-24
• [Presentation] チトクロム酸化酵素のリガンド解離構造ダイナミクス:新規高精度時間分解赤外分光装置を用いた追跡 (2011)
  • Author(s): 中島聡, その他
  • Organizer: 第38回生体分子科学討論会
  • Place of Presentation: 筑波大学、茨城県
  • Year and Date: 2011-06-24
• [Presentation] CO光解離をトリガーとしたチトクロムc酸化酵素の構造ダイナミクスの追跡 (2011)
  • Author(s): 石上泉, その他
  • Organizer: 第38回生体分子科学討論会
  • Place of Presentation: 筑波大学、茨城県
  • Year and Date: 2011-06-24
• [Presentation] 共鳴ラマン分光法によるインドールアミン2,3ジオキシゲナーゼの光学異性依存的基質相互作用の検出 (2011)
  • Author(s): 柳澤幸子, 他
  • Organizer: 第38回生体分子科学討論会
  • Place of Presentation: 筑波大学、茨城県
  • Year and Date: 2011-06-24
• [Presentation] ミオグロビンの機能調節機構の解明 (2011)
  • Author(s): 西村龍, その他
  • Organizer: 第38回生体分子科学討論会
  • Place of Presentation: 筑波大学、茨城県
  • Year and Date: 2011-06-23
• [Presentation] Resonance Raman st udy on CO-bound indoleamine 2, 3-dioxygenase (2011)
  • Author(s): Takashi Ogura
  • Organizer: The 3rd Georgian Bay International Conference on Bioi norganic Chemistry
  • Place of Presentation: Stockey Centre, Parry Sound, Ont ario, Canada
  • Year and Date: 2011-06-01
• [Presentation] Resonance Raman study on CO-bound indoleamine 2,3-dioxygenase (2011)
  • Author(s): Takashi Ogura
  • Organizer: The 3rd Georgian Bay International Conference on Bioinorganic Chemistry
  • Place of Presentation: Stockey Centre, Parry Sound, Ontario, Canada(招待講演)
  • Year and Date: 2011-06-01
• [Presentation] ヘム鉄の電子密度の変化を通したミオグロビンの機能調節機構の解明 (2011)
  • Author(s): 西村龍, その他
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(神奈川県)
  • Year and Date: 2011-03-29
• [Presentation] CuA近傍のアミノ酸残基へ部位特異的変異導入をしたチロシナーゼにおけるペルオキソ活性酸素種の特性評価 (2011)
  • Author(s): 藤枝伸宇, その他
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(神奈川県)
  • Year and Date: 2011-03-29
• [Presentation] CuB近傍のアミノ酸残基へ部位特異的変異を導入したチロシナーゼにおけるペルオキソ活性酸素種の特性評価 (2011)
  • Author(s): 藪田真太郎, その他
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(神奈川県)
  • Year and Date: 2011-03-29
• [Presentation] 単核ルテニウムヘテロポリタングステン酸錯体を触媒とする水の酸化反応機構 (2011)
  • Author(s): 村上将人, その他
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(神奈川県)
  • Year and Date: 2011-03-27
• [Presentation] 時間分解共鳴ラマン分光法によるCO光解離後のチトクロムc酸化酵素の構造緩和過程の追跡 (2011)
  • Author(s): 石上泉, その他
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(神奈川県)
  • Year and Date: 2011-03-27
• [Presentation] リン脂質小胞に再構成したチトクロムc酸化酵素の共鳴ラマン分光 (2011)
  • Author(s): 野村高志, その他
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(神奈川県)
  • Year and Date: 2011-03-27
• [Presentation] 高酸化型鉄ポルフィリンの共鳴ラマンスペクトル:軸配位子の陰性度及び2位の嵩高さがFe=O伸縮振動に及ぼす影響 (2011)
  • Author(s): 石丸裕士, その他
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(神奈川県)
  • Year and Date: 2011-03-27
• [Presentation] CO結合型インドールアミン2,3ジオキシゲナーゼへのTrpの光学異性体依存的相互作用の検出:共鳴ラマン分光法 (2011)
  • Author(s): 柳澤幸子, その他
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(神奈川県)
  • Year and Date: 2011-03-27
• [Presentation] 単核銅(II)アルキルペルオキソ錯体の性質に及ぼす配位子の効果 (2011)
  • Author(s): 太農哲朗, その他
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(神奈川県)
  • Year and Date: 2011-03-26
• [Presentation] Resonance Raman study on the oxygenated and the ferryl-oxo species of indoleamine 2,3-dioxygenase during catalytic turnover (2010)
  • Author(s): Takashi Ogura, et al.
  • Organizer: Faraday Discussions 148, Spectroscopy, Theory and Mechanisms in Bioinorganic Chemistry
  • Place of Presentation: University of Nottingham (UK)
  • Year and Date: 2010-07-06
• [Presentation] インドールアミンジオキシゲナーゼの共鳴ラマンスペクトル (2010)
  • Author(s): 柳澤幸子, その他
  • Organizer: 第37回生体分子科学討論会
  • Place of Presentation: 山口大学(山口県)
  • Year and Date: 2010-06-19
• [Presentation] Distinct binding geometries of CO to L-Trp- and D-Trp-bound indoleamine 2,3-dioxygenase as probed with resonance Raman spectroscopy (2010)
  • Author(s): Sachiko Yanagisawa, et al.
  • Organizer: Faraday Discussions 148, Spectroscopy, Theory and Mechanisms in Bioinorganic Chemistry
  • Place of Presentation: University of Nottingham (UK)
• [Presentation] Resonance Raman Identification of a Fe^<IV>=O Type Reaction Intermediate During Indoleamine 2,3-Dioxygenase Reaction (2010)
  • Author(s): Takashi Ogura, et al.
  • Organizer: The XXII^<th> International Conference on Raman Spectroscopy
  • Place of Presentation: Boston Park Plaza Hotel (USA)
• [Presentation] 高輝度フェムト秒レーザーを用いた時間分解赤外分光装置の開発 (2010)
  • Author(s): 久保稔, その他
  • Organizer: 分子科学討論会2010
  • Place of Presentation: 大阪大学(大阪府)
• [Presentation] ペルオキシダーゼ反応中間体モデル錯体の共鳴ラマンスペクトル (2010)
  • Author(s): 石丸裕士, その他
  • Organizer: 第60回錯体化学討論会
  • Place of Presentation: 大阪国際交流センター(大阪府)
• [Presentation] Femtosecond-Laser-Based Highly-Sensitive Time-Resolved IR Spectrometer for Studying Enzyme Reactions under Physiological Conditions (2010)
  • Author(s): Minoru Kubo, et al.
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学(宮城県)
• [Presentation] Resonance Raman study on the oxygenated and the ferryl-oxo species of indoleamine 2,3-dioxygenase (2010)
  • Author(s): Sachiko Yanagisawa, et al.
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学(宮城県)
• [Presentation] Interaction between Substrate and Dioxygen in the Enzyme/Substrate/Dioxygen Ternary Complex of Indoleamine Dioxygenase as Probed with Resonance Raman Spectroscopy (2010)
  • Author(s): Takashi Ogura, et al.
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学(宮城県)
• [Presentation] Resonance Raman Evidence for Heme-heme interaction in Cytochrome c Oxidase (2010)
  • Author(s): Miyuki Sakaguchi, et al.
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学(宮城県)
• [Presentation] Structural Analysis Transcriptional Factor NPAS2 by Resonance Raman Spectroscopy (2010)
  • Author(s): Yuko Misumi, et al.
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学(宮城県)
• [Presentation] Structural Relaxation of Cytochrome C Oxidase after CO photolysis : Assignment of a Heme Peripheral Raman Mode (2010)
  • Author(s): Izumi Ishigami, et al.
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学(宮城県)
• [Presentation] インドールアミンジオキシゲナーゼの反応中間体活性部位の構造解析:共鳴ラマン分光法 (2010)
  • Author(s): 小倉尚志
  • Organizer: 大阪大学蛋白質研究所セミナー:蛋白質の機能-構造相関解明のための精密構造解析とその方法～水素原子から細胞まで～
  • Place of Presentation: 大阪大学(大阪府)
• [Presentation] 代謝回転中インドールアミン2,3ジオキシゲナーゼの酸素化型およびフェリルオキソ種の検出 (2010)
  • Author(s): 柳澤幸子・堀谷正樹・杉本宏・城宜嗣・Appelman, Evan H.・小倉尚志
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 東大阪
• [Presentation] 高酸化型鉄ポルフィリンの共鳴ラマンスペクトル:Fe=O伸縮振動数の軸配位子依存性 (2010)
  • Author(s): 石丸裕士・藤井浩・小倉尚志
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 東大阪
• [Presentation] 共鳴ラマン分光法によるウシ心筋ミトコンドリア呼吸鎖NADH:ユビキノン酸化還元酵素のFMNと鉄-硫黄クラスターの構造解析 (2009)
  • Author(s): 引田理英, 森山昌和, 小倉尚志, 伊藤-新澤恭子, 吉川信也
  • Organizer: 第36回生体分子科学討論会
  • Place of Presentation: 札幌
• [Presentation] 共鳴ラマン分光法によるインドールアミン2,3-ジオキシナーゼの反応中間体の構造解析 (2009)
  • Author(s): 柳澤幸子, 四ッ谷景子, 橋脇裕美, 堀谷正樹, 杉本宏, 城宜嗣, 小倉尚志
  • Organizer: 第36回生体分子科学討論会
  • Place of Presentation: 札幌
• [Presentation] Structure and Dynamics of Cytochrome c Oxidase as Studied with Resonance Raman Spectroscopy (2009)
  • Author(s): 小倉尚志
  • Organizer: The 14^<th> International Conference on Biological Inorganic Chemistry(招待講演)
  • Place of Presentation: Nagoya
• [Presentation] Time-Resolved Resonance Raman Study on Structural Relaxation Process of Cytochrome c Oxidase Following Photolysis of Carbonmonoxide (2009)
  • Author(s): Izumi Ishigami, Satoru Nakashima, Kyoko Shinzawa-Itoh, Shinya Yoshikawa, Takashi Ogura
  • Organizer: The 14^<th> International Conference on Bioinorganic Chemistry
  • Place of Presentation: Nagoya
• [Presentation] A New Resonance Raman Marker Band of Cytochrome c Oxidase (2009)
  • Author(s): Miyuki Sakaguchi, Kyoko Shinzawa-Itoh, Shinya Yoshikawa, Hiroshi Fujii, Takashi Ogura
  • Organizer: The 14^<th> International Conference on Bioinorganic Chemistry
  • Place of Presentation: Nagoya
• [Presentation] X-ray Structure and Function of Peroxide Bridge between Fe and Cu in the O_2 Reduction Site of the Fully Oxidized Cytochrome c Oxidase (2009)
  • Author(s): Hiroshi Aoyama, Kazumasa Muramoto, Kyoko Shinzawa-Itoh, Kunio Hirata, Eiki Yamashita, Tomitake Tsukihara, Takashi Ogura, Shinya Yoshikawa
  • Organizer: The 14^<th> International Conference on Bioinorganic Chemistry
  • Place of Presentation: Nagoya
• [Presentation] Reactivity of Ru(IV)-Oxo Complexes Derived from Proton-Coupled Electron Transfer of Ru(II)-Aqua Complexes (2009)
  • Author(s): Takahiro Kojima, Yuichiro Hirai, Yasuhisa Mizutani, Kenichiro Ikemura, Takashi Ogura, Yoshihito Shiota, Kazunari Yoshizawa, Shunichi Fukuzumi
  • Organizer: The 14^<th> International Conference on Bioinorganic Chemistry
  • Place of Presentation: Nagoya
• [Presentation] Accurate Design of Copper-containing Oxidase Active Centers with a S-containing Ligand (2009)
  • Author(s): Takashi Tozuka, Yuji Kajita, Minoru Kubo, Takashi Ogura, Yasuhiro Funahashi, Tomohiro Ozawa, Hideki Masuda
  • Organizer: The 14^<th> International Conference on Bioinorganic Chemistry
  • Place of Presentation: Nagoya
• [Presentation] Structure and Reactivity of Mononuclear Copper(II)-Superoxo Complexes Supported by N_3-Tridentate Ligands (2009)
  • Author(s): Atsushi Kunishita, Hideki Sugimoto, Minoru Kubo, Takashi Ogura, Takeji Takui, Shinobu Itoh
  • Organizer: The 14^<th> International Conference on Bioinorganic Chemistry
  • Place of Presentation: Nagoya
• [Presentation] Preparation of Low-spin End-on Ferric-Peroxo and Ferric-Hydroperoxo Species by a Heme Model Complex Bearing an Imidazole Tail (2009)
  • Author(s): Jin-Gang Liu, Takehiro Ohta, Satoru Yamaguchi, Takashi Ogura, Yoshinori Naruta
  • Organizer: The 14^<th> International Conference on Bioinorganic Chemistry
  • Place of Presentation: Nagoya
• [Presentation] Detection of the Iron-Oxygen Stretching Raman Mode for Human Indoleamine 2, 3-Dioxygenase in the Oxygenated Form (2009)
  • Author(s): Sachiko Yanagisawa, Keiko Yotsuya, Masaki Horitani, Hiroshi Sugimoto, Yoshitsugu Shiro, Yumi Hashiwaki, Takashi Ogura
  • Organizer: The 14^<th> International Conference on Bioinorganic Chemistry
  • Place of Presentation: Nagoya
• [Presentation] C-H Bond Activation by Peroxodiiron(III)Complexes (2009)
  • Author(s): Susumu Kyuda, Yoshimi Kawade, Hideki Furutachi, Shuhei Fujinami, Masatatsu Suzuki, Takehiko Tosha, Teizo Kitagawa, Minoru Kubo, Takashi Ogura
  • Organizer: The 14^<th> International Conference on Bioinorganic Chemistry
  • Place of Presentation: Nagoya
• [Presentation] Contribution of the Supporting Ligands to NiO_2 Intermediates (2009)
  • Author(s): Jaeheung Cho, Ritimukta Sarangi, Jamespondi Annaraj, Sung Yeon Kim, Minoru Kubo, Takashi Ogura, Edward I.Solomon, Wonwoo Nam
  • Organizer: The 14^<th> International Conference on Bioinorganic Chemistry
  • Place of Presentation: Nagoya
• [Presentation] Cooperative L-Trp Binding to Human Tryptophan 2, 3-Dioxygenase Revealed by Resonance Raman Spectroscopy (2009)
  • Author(s): Eiko Fukumura, Hiroshi Sugimoto, Yuko Misumi, Takashi Ogura, Yoshitsugu Shiro
  • Organizer: The 14^<th> International Conference on Bioinorganic Chemistry
  • Place of Presentation: Nagoya
• [Presentation] Detection of the Iron-Oxygen Stretching Raman Mode for Human Indoleamine 2, 3-Dioxygenase in the Oxygenated Form (2009)
  • Author(s): 柳澤幸子
  • Organizer: International Workshop on Metalloprotein Functions
  • Place of Presentation: Kamigori, Hyogo
• [Presentation] Ultraviolet resonance Raman spectroscopy for functioning proteins (2009)
  • Author(s): 小倉尚志
  • Organizer: 第47回生物物理学会年会(招待講演)
  • Place of Presentation: 徳島
• [Presentation] Site-directed isotope labeling of cytochrome c oxidase (2009)
  • Author(s): Hideo Shimada, Yukie Katayama, Takashi Ogura, Tomitake Tsukihara, Shinya Yoshikawa
  • Organizer: 第47回生物物理学会年会(招待講演)
  • Place of Presentation: 徳島
• [Presentation] Resonance Raman Marker Bands of Hydroxyfarnesylethyl Substituent of Cytochrome c Oxidase (2009)
  • Author(s): Miyuki Sakaguchi, Kyoko Shinzawa-Itoh, Shinya Yoshikawa, Hiroshi Fujii, Takashi Ogura
  • Organizer: 第47回生物物理学会年会
  • Place of Presentation: 徳島
• [Presentation] Structural Relaxation Process of Cytochrome c Oxidase Following Photolysis of Carbonmonoxide (2009)
  • Author(s): Izumi Ishigami, Satoru Nakashima, Kyoko Shinzawa-Itoh, Shinya Yoshikawa, Takashi Ogura
  • Organizer: 第47回生物物理学会年会
  • Place of Presentation: 徳島
• [Presentation] Structural analysis of FMN and iron-sulfer clusters of the bovine heart NADH-ubiquinone oxidoreductase by resonance Raman spectra (2009)
  • Author(s): 引田理英, 森山昌和, 小倉尚志, 伊藤-新澤恭子, 吉川信也
  • Organizer: 第47回生物物理学会年会
  • Place of Presentation: 徳島
• [Presentation] Non-Resonance Raman Investigations of Bovine Mitochondrial F_oF_1-ATPsynthase (2009)
  • Author(s): Kaoru Mieda, Minoru Kubo, Kyoko Shinzawa-Itoh, Takashi Ogura, Shinya Yoshikawa
  • Organizer: 第47回生物物理学会年会
  • Place of Presentation: 徳島
• [Presentation] Detection of Two Oxygen-Isotope Sensitive Resonance Raman Bands for Human Indoleamine 2, 3-Dioxygenase (2009)
  • Author(s): 柳澤幸子, 四ッ谷景子, 橋脇裕美, 堀谷正樹, 杉本宏, 城宜嗣, 小倉尚志
  • Organizer: 第47回生物物理学会年会
  • Place of Presentation: 徳島
• [Presentation] Protein Dynamics of Cytochrome c Oxidase as Studied with Resonance Raman Spectroscopy (2009)
  • Author(s): Takashi Ogura, Satoru Nakashima, Izumi Ishigami, Kyoko Shinzawa-Itoh, Shinya Yoshikawa
  • Organizer: Symposium on Advanced Biological Inorganic Chemistry(招待講演)
  • Place of Presentation: Mumbai, India
• [Remarks] ホームページ:(研究一覧:
  • URL: http://lib.laic.u-hyogo.ac.jp/laic/2/annual_list.html
• [Remarks] 市民講演会開催「ピコバイオロジー:原子レベルの生命科学」「ナノ」よりミクロな「ピコ」、「ピコバイオロジー」ってなんだろう。第49回日本生物物理学会年会実行委員会主催、平成23年9月19日、姫路商工会議所、兵庫県。
  • URL: http://www.sci.u-hyogo.ac.jp/life/GCOE/index-j.html
• [Remarks]
  • URL: http://www.sci.u-hyogo.ac.jp/
• [Remarks]
  • URL: http://www.sci.u-hyogo.ac.jp/life/biophys2/MolBiophys_II_main/home_j.html
• [Remarks]
  • URL: http://www.sci.u-hyogo.ac.jp/life/biophys2/MolBiophys_II_main/home.html