Study of rhodopsin-ion pumps by electrical measurement focusing on proteorhodopsin

• Project/Area Number: 21570172
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Chuo University
• Principal Investigator: MUNEYUKI Eiro 中央大学, 理工学部, 教授 (80219865)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 光生物学 / 生体エネルギー論 / 分子機械 / 生物物理 / エネルギー全般 / 生体分子

Research Abstract

In the present study, we examined the photoelectric current generated by bacteriorhodopsin adsorbed on a thin polymer film. An analysis of the experimental results obtained at various light intensities suggested that the electromotive force generated by bacteriorhodopsin was independent of the light intensity. The pH dependency of the photoelectric current suggested that the bacteriorhodopsin could generate the maximum electromotive force at around pH 6.We constructed a mutant of proteorhodopsin which mimics bacteriorhodopsin and found a change in the order of proton uptake and release. We also constructed a general model for ion pumps.

Research Products

• [Journal Article] Recovery of state-specific potential of molecular motor from single-molecule trajectory (2012)
  • Author(s): Shoichi Toyabe, Hiroshi Ueno, Eiro Muneyuki
  • Journal Title: EPL Volume: 97 Issue: 4 Pages: 40004-40004
  • DOI: 10.1209/0295-5075/97/40004
  • Peer Reviewed
• [Journal Article] Allosteric model of anion pump (2010)
  • Author(s): Muneyuki Eiro, Sekimoto Ken
  • Journal Title: Physical Review E Volume: 81巻
  • Peer Reviewed
• [Journal Article] Nonequilibrium Energetics of a Single F_1-ATPase Molecule (2010)
  • Author(s): Toyabe, S., Okamoto, T., Watanabe-Nakayama, T., Taketani, H., Kudo, S., Muneyuki, E.
  • Journal Title: Phys.Rev.Lett. Volume: 104 Pages: 198103-198103
  • Peer Reviewed
• [Journal Article] Chemo-mechanical coupling in F_1-ATPase revealed by catalytic site occupancy during catalysis. (2010)
  • Author(s): Shimo-Kon R, Muneyuki E, Sakai H, Adachi K, Yoshida M, Kinosita K Jr.
  • Journal Title: Biophys J. Volume: 98 Pages: 1227-36
  • Peer Reviewed
• [Journal Article] Experimental demonstration of information-to-energy conversion and validation of the generalized Jarzynski equality (2010)
  • Author(s): Toyabe, S., Sagawa, T., Ueda, M., Muneyuki, E., Sano, M
  • Journal Title: Nature Physics Volume: 6 Pages: 988-992
  • Peer Reviewed
• [Journal Article] Observation of DNA pinning at laser focal point on Au surface and its application to single DNA nanowire and cross-wire formation. (2010)
  • Author(s): Fujii, S.Kobayashi, K., Kanaizuka, K., Okamoto, T., Toyabe, S., Muneyuki, E., Haga, M.
  • Journal Title: Bioelectrochemistry Volume: 80 Pages: 26-30
  • Peer Reviewed
• [Journal Article] Allosteric model of an ion pump (2010)
  • Author(s): Muneyuki Eiro, Sekimoto Ken
  • Journal Title: Physical Review E 81
  • Peer Reviewed
• [Journal Article] Manipulation of Single DNA Using a Micronanobubble Formed by Local Laser Heating on a Au-coated Surface (2010)
  • Author(s): Sho Fujii, Katsuaki Kobayashi, Katsuhiko Kanaizuka, Tetsuaki Okamoto, Shoichi Toyabe, Eiro Muneyuki, Masa-aki Haga
  • Journal Title: Chemistry Letters 39(2) Pages: 92-93
  • NAID: 10027176924
  • Peer Reviewed
• [Journal Article] Modulation of nucleotide binding to the catalytic sites of thermophilic F1-ATPase by the ε subunit : Implication for the role of the a subunit in ATP synthesis (2009)
  • Author(s): Yasuno, T., Muneyuki, E., Yoshida, M., Kato-Yamada, Y.
  • Journal Title: Biochem Biophys Res Commun 390(2) Pages: 230-234
  • Peer Reviewed
• [Presentation] pH dependency of photoelectric current of bacteriorhodopsin absorbed on Lumirror membrane (2011)
  • Author(s): 宮崎師夫, 鳥谷部祥一, 上野博史, 宗行英朗
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学
  • Year and Date: 2011-09-18
• [Presentation] Molecular dynamics simulation of outer mitochondrial membrane protein Tom20-presequence complexミトコンドリア外膜タンパク質Tom20-プレ配列ペプチド複合体の分子動力学計算 (2011)
  • Author(s): 小室靖明, 森貴治, 宗行英朗, 齊藤貴士, 神田大輔, 杉田有治
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学
  • Year and Date: 2011-09-18
• [Presentation] The fast proton release of proteorhodopsin at low pH低pH条件下におけるプロテオロドプシンの速いプロトン放出について (2011)
  • Author(s): 田母神淳, 菊川峰志, 下野和実, 奈良敏文, 宗行英朗, 加茂直樹
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学
  • Year and Date: 2011-09-17
• [Presentation] Single-molecule nonequilibrium thermodynamics of molecular motor分子モーターの1分子非平衡熱力学 (2011)
  • Author(s): 鳥谷部祥一, 上野博史, 宗行英朗
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学
  • Year and Date: 2011-09-16
• [Presentation] Simultaneous observation of fluorescent-nucleotides and rotation of F1-ATPase using plasmonic gold nanoparticle表面プラズモン共鳴を引き起こす金微粒子を用いた蛍光ヌクレオチドとF1-ATPaseの回転の同時観察 (2011)
  • Author(s): 上野博史, 鳥谷部祥一, 宗行英朗
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学
  • Year and Date: 2011-09-16
• [Presentation] Thermodynamic parameters of nucleotide binding to the catalytic sites of F1-ATPase. F1-ATPaseの触媒部位に対するヌクレオチド結合の温度依存性 (2011)
  • Author(s): 菊池洋輔, 中裕佑, 小酒部秀光, 宗行英朗
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学
  • Year and Date: 2011-09-16
• [Presentation] Information-heat engine as a model system of molecular motors (2010)
  • Author(s): Toyabe, S., Sagawa, T., Ueda, M., Muneyuki, E., Sano, M.
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学川内キャンパス
  • Year and Date: 2010-09-22
• [Presentation] ヌクレオチドとF1-ATPaseとの結合の熱力学パラメータの測定Thermodynamic parameters of nucleotide binding to the catalytic sites of F1-ATPase revealed by fluorescence measurement (2010)
  • Author(s): Kikuchi, Y., Naka, Y., Osakabe, H., Toyabe, S., Ueno, H., Muneyuki, E.
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学川内キャンパス
  • Year and Date: 2010-09-22
• [Presentation] F1-ATPaseのβサブユニット(Y341W)単体へのヌクレオチド結合の温度依存性Temperature dependence of nucleotide binding to β subunit of F1-ATPase revealed by fluorescence measurement (2010)
  • Author(s): Naka, Y., Osakabe, H., Masaike, T., Okamoto, T., Muneyuki, E.
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学川内キャンパス
  • Year and Date: 2010-09-22
• [Presentation] 2 mM ATPのDMSO存在下におけるF1-ATPaseのステップ運動Stepwise rotation of F1-ATPase at 2 mM ATP in the presence of DMSO (2010)
  • Author(s): Nishizuka, K., Toyabe, S., Okamoto, T., Muneyuki, E.
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学川内キャンパス
  • Year and Date: 2010-09-22
• [Presentation] ミトコンドリア外膜タンパク質Tom20-プレ配列複合体の分子動力学計算Molecular dynamics simulation of outer mitochondrial protein Tom20-presequence complex (2010)
  • Author(s): Komuro, Y., Mori, T., Muneyuki, M., Saito, T., Kohda, D., Sugita, Y.
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学川内キャンパス
  • Year and Date: 2010-09-21
• [Presentation] ハイブリッドF1-ATPaseの角度解析によって見出されたもう一つのサブ-サブステップ回転Angle distribution analysis of hybrid F1-ATPase indicates another sub-substep rotation between two catalytic dwells (2010)
  • Author(s): Ariga, T., Muneyuki, E., Yoshida, M.
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学川内キャンパス
  • Year and Date: 2010-09-20
• [Presentation] 2mM ATPにおけるDNSO下でのF1-ATPaseのステップ運動 Stepping rotation of F1-ATPase at 2mM ATP in the presence of DMSO(dimethylsulfoxide) (2009)
  • Author(s): Kazuhiro Nishizuka, Shoiti Toyabe, Tetsuaki Okamoto, Eiro Muneyuki
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: アスティ徳島(徳島)
  • Year and Date: 2009-11-01
• [Presentation] F1-ATPaseのβ-サブユニット(Y341W)単体へのヌクレオチド結合の温度依存性 Temperature dependence of nucleotide binding to β-subunit of F1-ATPase revealed by fluorescence measurement (2009)
  • Author(s): Yusuke Naka, Eiro Muneyuki, Hidemitsu Osakabe, Tomoko Masaike
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: アスティ徳島(徳島)
  • Year and Date: 2009-11-01
• [Presentation] F1-ATPaseのストールトルク Stall torque of F1-ATPase (2009)
  • Author(s): Toyabe, S., Watanabe-Nakayama, T., Okamoto, T., Kudo, S., Muneyuki, E.
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: アスティ徳島(徳島)
  • Year and Date: 2009-10-31
• [Presentation] F1-ATPaseの温度高感受性待ち時間の一分子速度論解析 Single molecule kinetic analysis of the temperature sensitive dwell of F1-ATPase (2009)
  • Author(s): Tetsuaki Okamoto, Shouich Toyabe, Eiro Muneyuki
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: アスティ徳島(徳島)
  • Year and Date: 2009-10-30