Study of rhodopsin-ion pumps by electrical measurement focusing on proteorhodopsin
| Project/Area Number |
21570172
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Chuo University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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|---|
| Keywords | 光生物学 / 生体エネルギー論 / 分子機械 / 生物物理 / エネルギー全般 / 生体分子 |
|---|
| Research Abstract |
In the present study, we examined the photoelectric current generated by bacteriorhodopsin adsorbed on a thin polymer film. An analysis of the experimental results obtained at various light intensities suggested that the electromotive force generated by bacteriorhodopsin was independent of the light intensity. The pH dependency of the photoelectric current suggested that the bacteriorhodopsin could generate the maximum electromotive force at around pH 6.We constructed a mutant of proteorhodopsin which mimics bacteriorhodopsin and found a change in the order of proton uptake and release. We also constructed a general model for ion pumps.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Nonequilibrium Energetics of a Single F_1-ATPase Molecule2010
Author(s)
Toyabe, S., Okamoto, T., Watanabe-Nakayama, T., Taketani, H., Kudo, S., Muneyuki, E.
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Journal Title
Phys.Rev.Lett.
Volume: 104
Pages: 198103-198103
Related Report
Peer Reviewed
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[Journal Article] Observation of DNA pinning at laser focal point on Au surface and its application to single DNA nanowire and cross-wire formation.2010
Author(s)
Fujii, S.Kobayashi, K., Kanaizuka, K., Okamoto, T., Toyabe, S., Muneyuki, E., Haga, M.
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Journal Title
Bioelectrochemistry
Volume: 80
Pages: 26-30
Related Report
Peer Reviewed
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[Presentation] ヌクレオチドとF1-ATPaseとの結合の熱力学パラメータの測定Thermodynamic parameters of nucleotide binding to the catalytic sites of F1-ATPase revealed by fluorescence measurement2010
Author(s)
Kikuchi, Y., Naka, Y., Osakabe, H., Toyabe, S., Ueno, H., Muneyuki, E.
Organizer
日本生物物理学会第48回年会
Place of Presentation
東北大学川内キャンパス
Year and Date
2010-09-22
Related Report
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[Presentation] ミトコンドリア外膜タンパク質Tom20-プレ配列複合体の分子動力学計算Molecular dynamics simulation of outer mitochondrial protein Tom20-presequence complex2010
Author(s)
Komuro, Y., Mori, T., Muneyuki, M., Saito, T., Kohda, D., Sugita, Y.
Organizer
日本生物物理学会第48回年会
Place of Presentation
東北大学川内キャンパス
Year and Date
2010-09-21
Related Report
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