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Study of rhodopsin-ion pumps by electrical measurement focusing on proteorhodopsin

Research Project

Project/Area Number 21570172
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionChuo University

Principal Investigator

MUNEYUKI Eiro  中央大学, 理工学部, 教授 (80219865)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords光生物学 / 生体エネルギー論 / 分子機械 / 生物物理 / エネルギー全般 / 生体分子
Research Abstract

In the present study, we examined the photoelectric current generated by bacteriorhodopsin adsorbed on a thin polymer film. An analysis of the experimental results obtained at various light intensities suggested that the electromotive force generated by bacteriorhodopsin was independent of the light intensity. The pH dependency of the photoelectric current suggested that the bacteriorhodopsin could generate the maximum electromotive force at around pH 6.We constructed a mutant of proteorhodopsin which mimics bacteriorhodopsin and found a change in the order of proton uptake and release. We also constructed a general model for ion pumps.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (25 results)

All 2012 2011 2010 2009

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (16 results)

  • [Journal Article] Recovery of state-specific potential of molecular motor from single-molecule trajectory2012

    • Author(s)
      Shoichi Toyabe, Hiroshi Ueno, Eiro Muneyuki
    • Journal Title

      EPL

      Volume: 97 Issue: 4 Pages: 40004-40004

    • DOI

      10.1209/0295-5075/97/40004

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Allosteric model of anion pump2010

    • Author(s)
      Muneyuki Eiro, Sekimoto Ken
    • Journal Title

      Physical Review E

      Volume: 81巻

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nonequilibrium Energetics of a Single F_1-ATPase Molecule2010

    • Author(s)
      Toyabe, S., Okamoto, T., Watanabe-Nakayama, T., Taketani, H., Kudo, S., Muneyuki, E.
    • Journal Title

      Phys.Rev.Lett.

      Volume: 104 Pages: 198103-198103

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chemo-mechanical coupling in F_1-ATPase revealed by catalytic site occupancy during catalysis.2010

    • Author(s)
      Shimo-Kon R, Muneyuki E, Sakai H, Adachi K, Yoshida M, Kinosita K Jr.
    • Journal Title

      Biophys J.

      Volume: 98 Pages: 1227-36

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Experimental demonstration of information-to-energy conversion and validation of the generalized Jarzynski equality2010

    • Author(s)
      Toyabe, S., Sagawa, T., Ueda, M., Muneyuki, E., Sano, M
    • Journal Title

      Nature Physics

      Volume: 6 Pages: 988-992

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Observation of DNA pinning at laser focal point on Au surface and its application to single DNA nanowire and cross-wire formation.2010

    • Author(s)
      Fujii, S.Kobayashi, K., Kanaizuka, K., Okamoto, T., Toyabe, S., Muneyuki, E., Haga, M.
    • Journal Title

      Bioelectrochemistry

      Volume: 80 Pages: 26-30

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Allosteric model of an ion pump2010

    • Author(s)
      Muneyuki Eiro, Sekimoto Ken
    • Journal Title

      Physical Review E 81

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Manipulation of Single DNA Using a Micronanobubble Formed by Local Laser Heating on a Au-coated Surface2010

    • Author(s)
      Sho Fujii, Katsuaki Kobayashi, Katsuhiko Kanaizuka, Tetsuaki Okamoto, Shoichi Toyabe, Eiro Muneyuki, Masa-aki Haga
    • Journal Title

      Chemistry Letters 39(2)

      Pages: 92-93

    • NAID

      10027176924

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Modulation of nucleotide binding to the catalytic sites of thermophilic F1-ATPase by the ε subunit : Implication for the role of the a subunit in ATP synthesis2009

    • Author(s)
      Yasuno, T., Muneyuki, E., Yoshida, M., Kato-Yamada, Y.
    • Journal Title

      Biochem Biophys Res Commun 390(2)

      Pages: 230-234

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] pH dependency of photoelectric current of bacteriorhodopsin absorbed on Lumirror membrane2011

    • Author(s)
      宮崎師夫, 鳥谷部祥一, 上野博史, 宗行英朗
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学
    • Year and Date
      2011-09-18
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Molecular dynamics simulation of outer mitochondrial membrane protein Tom20-presequence complexミトコンドリア外膜タンパク質Tom20-プレ配列ペプチド複合体の分子動力学計算2011

    • Author(s)
      小室靖明, 森貴治, 宗行英朗, 齊藤貴士, 神田大輔, 杉田有治
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学
    • Year and Date
      2011-09-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] The fast proton release of proteorhodopsin at low pH低pH条件下におけるプロテオロドプシンの速いプロトン放出について2011

    • Author(s)
      田母神淳, 菊川峰志, 下野和実, 奈良敏文, 宗行英朗, 加茂直樹
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学
    • Year and Date
      2011-09-17
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Single-molecule nonequilibrium thermodynamics of molecular motor分子モーターの1分子非平衡熱力学2011

    • Author(s)
      鳥谷部祥一, 上野博史, 宗行英朗
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Simultaneous observation of fluorescent-nucleotides and rotation of F1-ATPase using plasmonic gold nanoparticle表面プラズモン共鳴を引き起こす金微粒子を用いた蛍光ヌクレオチドとF1-ATPaseの回転の同時観察2011

    • Author(s)
      上野博史, 鳥谷部祥一, 宗行英朗
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Thermodynamic parameters of nucleotide binding to the catalytic sites of F1-ATPase. F1-ATPaseの触媒部位に対するヌクレオチド結合の温度依存性2011

    • Author(s)
      菊池洋輔, 中裕佑, 小酒部秀光, 宗行英朗
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Information-heat engine as a model system of molecular motors2010

    • Author(s)
      Toyabe, S., Sagawa, T., Ueda, M., Muneyuki, E., Sano, M.
    • Organizer
      日本生物物理学会第48回年会
    • Place of Presentation
      東北大学川内キャンパス
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヌクレオチドとF1-ATPaseとの結合の熱力学パラメータの測定Thermodynamic parameters of nucleotide binding to the catalytic sites of F1-ATPase revealed by fluorescence measurement2010

    • Author(s)
      Kikuchi, Y., Naka, Y., Osakabe, H., Toyabe, S., Ueno, H., Muneyuki, E.
    • Organizer
      日本生物物理学会第48回年会
    • Place of Presentation
      東北大学川内キャンパス
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] F1-ATPaseのβサブユニット(Y341W)単体へのヌクレオチド結合の温度依存性Temperature dependence of nucleotide binding to β subunit of F1-ATPase revealed by fluorescence measurement2010

    • Author(s)
      Naka, Y., Osakabe, H., Masaike, T., Okamoto, T., Muneyuki, E.
    • Organizer
      日本生物物理学会第48回年会
    • Place of Presentation
      東北大学川内キャンパス
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] 2 mM ATPのDMSO存在下におけるF1-ATPaseのステップ運動Stepwise rotation of F1-ATPase at 2 mM ATP in the presence of DMSO2010

    • Author(s)
      Nishizuka, K., Toyabe, S., Okamoto, T., Muneyuki, E.
    • Organizer
      日本生物物理学会第48回年会
    • Place of Presentation
      東北大学川内キャンパス
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] ミトコンドリア外膜タンパク質Tom20-プレ配列複合体の分子動力学計算Molecular dynamics simulation of outer mitochondrial protein Tom20-presequence complex2010

    • Author(s)
      Komuro, Y., Mori, T., Muneyuki, M., Saito, T., Kohda, D., Sugita, Y.
    • Organizer
      日本生物物理学会第48回年会
    • Place of Presentation
      東北大学川内キャンパス
    • Year and Date
      2010-09-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] ハイブリッドF1-ATPaseの角度解析によって見出されたもう一つのサブ-サブステップ回転Angle distribution analysis of hybrid F1-ATPase indicates another sub-substep rotation between two catalytic dwells2010

    • Author(s)
      Ariga, T., Muneyuki, E., Yoshida, M.
    • Organizer
      日本生物物理学会第48回年会
    • Place of Presentation
      東北大学川内キャンパス
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 2mM ATPにおけるDNSO下でのF1-ATPaseのステップ運動 Stepping rotation of F1-ATPase at 2mM ATP in the presence of DMSO(dimethylsulfoxide)2009

    • Author(s)
      Kazuhiro Nishizuka, Shoiti Toyabe, Tetsuaki Okamoto, Eiro Muneyuki
    • Organizer
      第47回日本生物物理学会年会
    • Place of Presentation
      アスティ徳島(徳島)
    • Year and Date
      2009-11-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] F1-ATPaseのβ-サブユニット(Y341W)単体へのヌクレオチド結合の温度依存性 Temperature dependence of nucleotide binding to β-subunit of F1-ATPase revealed by fluorescence measurement2009

    • Author(s)
      Yusuke Naka, Eiro Muneyuki, Hidemitsu Osakabe, Tomoko Masaike
    • Organizer
      第47回日本生物物理学会年会
    • Place of Presentation
      アスティ徳島(徳島)
    • Year and Date
      2009-11-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] F1-ATPaseのストールトルク Stall torque of F1-ATPase2009

    • Author(s)
      Toyabe, S., Watanabe-Nakayama, T., Okamoto, T., Kudo, S., Muneyuki, E.
    • Organizer
      第47回日本生物物理学会年会
    • Place of Presentation
      アスティ徳島(徳島)
    • Year and Date
      2009-10-31
    • Related Report
      2009 Annual Research Report
  • [Presentation] F1-ATPaseの温度高感受性待ち時間の一分子速度論解析 Single molecule kinetic analysis of the temperature sensitive dwell of F1-ATPase2009

    • Author(s)
      Tetsuaki Okamoto, Shouich Toyabe, Eiro Muneyuki
    • Organizer
      第47回日本生物物理学会年会
    • Place of Presentation
      アスティ徳島(徳島)
    • Year and Date
      2009-10-30
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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