Molecular Mechanism of re-isomerization ofretinal in visual cycle

• Project/Area Number: 21570174
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Tokushima Bunri University
• Principal Investigator: TSUDA Motoyuki 徳島文理大学, 香川薬学部, 客員教授 (60045458)
• Co-Investigator(Renkei-kenkyūsha): TERAKITA Akihisa 大阪市立大学, 理学研究科, 教授 (30212062) ; KUSAKABE Takehiro 甲南大学, 理工学部, 教授 (40280862)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 視覚サイクル / レチナール / ロドプシン / 異性化 / ホヤ / 光異性化酵素 / 抗体 / 暗異性化酵素

Research Abstract

Absorption of a photon by visual pigments induces isomerization of 11-cis-retinaldehyde(RAL) chromophore to all-trans-RAL. Here we comparevisual retinoid cycles between different photoreceptors of vertebrates as well as invertebrates. The visual cycle systems in ascidians, the closest living relatives of vertebrates, show an intermediate state between vertebrates and non-chordate invertebrates. The ascidian larva may use retinochrome-like opsin as the major isomerase retinal but RPE65 in asidinan did not isomerase retinal. These results are very interesting in the evolutional standpoints.

Research Products

• [Journal Article] Evidence for differential regulation of GnRH signaling via heterodimerization amongGnRH receptor paralogs in the protochordate (2012)
  • Author(s): T. Sakai, M. Aoyama, T. Kusakabe, M. Tsuda, H. Satake
  • Journal Title: Ciona, Endocrinology Volume: 153 Pages: 1841-1849
  • Peer Reviewed
• [Journal Article] Evidence for differential regulation of GnRH signaling via heterodimerization amongGnRH receptor paralogs in the protochordate, Ciona intestinalis (2012)
  • Author(s): T.Sakai, M.Aoyama, T.Kusakabe, M.Tsuda, H.Satake
  • Journal Title: Endocrinology Volume: 153 Pages: 1841-1849
  • Peer Reviewed
• [Journal Article] Functional Diversity of Signaling Pathways through G Protein-CoupledReceptor Heterodimerization with a Species-Specific Orphan Receptor Subtype (2010)
  • Author(s): Sakai, T., Aoyama, M., Kusakabe, K., Tsuda, M., Satake, H
  • Journal Title: Mol. Biol. Evol Volume: 27(5) Pages: 1097-1106
  • Peer Reviewed
• [Journal Article] Smith WC, doublesex/mab3 related-1(dmrt1) is essential for development of anterior neural plate derivatives in Ciona (2010)
  • Author(s): Tresser J, Chiba S, Veeman M, El-Nachef D, Newman-Smith E, Horie T, Tsuda M
  • Journal Title: Development Volume: 37(13) Pages: 197-203
  • Peer Reviewed
• [Journal Article] Simple motor systemof the ascidian larva : neuronal complexcomprising putative cholinergic andGABAergic/glycinergic neurons (2010)
  • Author(s): Horie T, Nakagawa M, Sasakura Y, Kusakabe TG, Tsuda M
  • Journal Title: Zoolog Sci Volume: 27(2) Pages: 181-90
  • Peer Reviewed
• [Journal Article] Functional Diversity of Signaling Pathways through G Protein-CoupledReceptor Heterodimerization with a Species-Specific Orphan Receptor Subtype (2010)
  • Author(s): Sakai, T., Aoyama,M., Kusakabe, K., Tsuda, M., Satake, H
  • Journal Title: Mol.Biol.Evol. Volume: 27(5) Pages: 1097-1106
  • Peer Reviewed
• [Journal Article] doublesex/mab3 related-1 (dmrt1) is essential for development of anterior neural plate derivatives in Ciona. (2010)
  • Author(s): Tresser J, Chiba S, Veeman M, El-Nachef D, Newman-Smith E, Horie T, Tsuda M, Smith WC
  • Journal Title: Development Volume: 37(13) Pages: 2197-2203
  • Peer Reviewed
• [Journal Article] Simple motor system of the ascidian larva : neuronal complex comprising putative cholinergic and GABAergic/glycinergic neurons. (2010)
  • Author(s): Horie T, Nakagawa M, Sasakura Y, Kusakabe TG, Tsuda M
  • Journal Title: Zoolog Sci. Volume: 27(2) Pages: 181-190
  • NAID: 40017358569
  • Peer Reviewed
• [Journal Article] Functional Diversity of Signaling Pathways through G Protein-CoupledReceptor Heterodimerization with a Species-Specific Orphan Receptor Subtype (2010)
  • Author(s): Sakai, T., Aoyama, M., Kusakabe, K., Tsuda, M., Satake, H
  • Journal Title: Mol.Biol.Evol. 27(5) Pages: 1097-1106
  • Peer Reviewed
• [Journal Article] Evolution and the originof the visual retinoid cycle invertebrates (2009)
  • Author(s): Kusakabe, T., Takimoto, N., Minghao Jin, M., Tsuda, M
  • Journal Title: Phil. Trans. R. Soc. Lond Volume: 364 Pages: 2897-2910
  • Peer Reviewed
• [Journal Article] Tube formation by complexcellular processes in Ciona intestinalis notochord (2009)
  • Author(s): Dong, B., Horie, T., Denker, E., Kusakabe, K., Tsuda, M., Smith, W., Jiang, D.
  • Journal Title: Dev. Biol Volume: 330(2) Pages: 237-249
  • Peer Reviewed
• [Journal Article] Evolution and the origin of the visual retinoid cycle in vertebrates (2009)
  • Author(s): Kusakabe, T., Takimoto, N., Minghao Jin, M., Tsuda, M.
  • Journal Title: Phil.Trans.R.Soc.Lond. B 364 Pages: 2897-2910
  • Peer Reviewed
• [Journal Article] Tube formation by complex cellular processes in Ciona intestinalis notochord (2009)
  • Author(s): Dong, B., Horie, T., Denker, E., Kusakabe, K., Tsuda, M., Smith, W., Jiang, D.
  • Journal Title: Dev.Biol. 330(2) Pages: 237-249
  • Peer Reviewed
• [Presentation] 視覚サイクルの分子メカニズム (2010)
  • Author(s): 津田基之
  • Organizer: 生物物理学会中国四国支部大会
  • Place of Presentation: 松山大学
• [Presentation] Crystalization of octopus rhodopsin (2009)
  • Author(s): Zang, Kanda, Tsuda,, Kouyama
  • Organizer: 生物物理学会
  • Place of Presentation: アスティ徳島。徳島市
  • Year and Date: 2009-11-01
• [Presentation] Crystalization of octopus rhodopsin (2009)
  • Author(s): Zang, Kanda, Tsuda, Kouyama
  • Organizer: 生物物理学会
  • Place of Presentation: アスティ徳島、徳島市
• [Book] 環境生物学.地球環境を守るには (2010)
  • Author(s): 針山孝彦、津田基之
  • Total Pages: 261
  • Publisher: 共立出版
• [Book] 環境生物学 (2010)
  • Author(s): 針山孝彦、津田基之
  • Total Pages: 258
  • Publisher: 共立出版
• [Book] 環境生物学-地球環境を守るには (2010)
  • Author(s): 針山孝彦、津田基之
  • Total Pages: 261
  • Publisher: 共立出版