Gene-specific transcriptional regulation by heat shock factor

• Project/Area Number: 21570181
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Kanazawa University
• Principal Investigator: SAKURAI Hiroshi 金沢大学, 保健学系, 准教授 (00225848)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ストレス応答 / 熱ショック転写因子 / 培養細胞 / 酵母菌

Research Abstract

HSF(heat shock factor) trimer, an evolutionarily conserved heat-responsive regulator, binds to heat shock element(HSE) and regulates expression of target genes. Continuous HSEs consist of contiguous inverted repeats of the nGAAn sequence, and discontinuous HSEs contain gaps in the array of the unit. This study shows that HSFs of various organisms, including human, bind discontinuous HSEs, as well as continuous HSEs. Furthermore, modulation of the trimer formation is important for the HSE specificity of HSFs.

Research Products

• [Journal Article] Regulation of chaperone gene expression by heat shock transcription factor in Saccharomyces cerevisiae : Importance in normal cell growth, stress resistance and longevity (2011)
  • Author(s): Sakurai, H., Ota, A.
  • Journal Title: FEBS Letters Volume: 585 Pages: 2744-2748
  • Peer Reviewed
• [Journal Article] Binding affinities and interactions among different heat shock element types and heat shock factors in rice(Oryza sativa L.) (2011)
  • Author(s): Mittal, DJ., Enoki, Y., Lavania, D., Singh, A., Sakurai, H., Grover, A.
  • Journal Title: FEBS Journal Volume: 278 Pages: 3076-3085
  • Peer Reviewed
• [Journal Article] Diversity in DNA recognition by heat shock transcription factors(HSFs) from model organisms (2011)
  • Author(s): Enoki, Y., Sakurai, H.
  • Journal Title: FEBS Letters Volume: 585 Pages: 1293-1298
  • Peer Reviewed
• [Journal Article] Regulation of chaperone gene expression by heat shock transcription factor in Saccharomyces cerevisiae (2011)
  • Author(s): Sakurai, H.
  • Journal Title: FEBS Letters Volume: 585 Issue: 17 Pages: 2744-2748
  • DOI: 10.1016/j.febslet.2011.07.041
  • Peer Reviewed
• [Journal Article] Diversity in DNA recognition by heat shock transcription fiactors (HSFs) from model organisms (2011)
  • Author(s): Enoki, Y.
  • Journal Title: FEBS Letters Volume: 585 Issue: 9 Pages: 1293-1298
  • DOI: 10.1016/j.febslet.2011.04.014
  • Peer Reviewed
• [Journal Article] Binding affinities and interactions among different heat shock element types and heat shock factors in rice (Oryza sativa L.) (2011)
  • Author(s): Mittal, DJ.
  • Journal Title: FEBS Journal Volume: 278 Issue: 17 Pages: 3076-3085
  • DOI: 10.1111/j.1742-4658.2011.08229.x
  • Peer Reviewed
• [Journal Article] Novel aspects of heat shock factors : DNA recognition, chromatin modulation and gene expression (2010)
  • Author(s): Sakurai, H., Enoki, Y.
  • Journal Title: FEBS Journal Volume: 277 Pages: 4140-4149
  • Peer Reviewed
• [Journal Article] DNA-binding and transcriptional activities of human HSF4 containing mutations that associate with congenital and age-related cataracts (2010)
  • Author(s): Enoki, Y., Mukoda, Y., Furutani, C., Sakurai, H.
  • Journal Title: Biochimica et Biophysica Acta Molecular Basis of Disease Volume: 1802 Pages: 749-753
  • Peer Reviewed
• [Journal Article] Mutational analysis of human heat-shock transcription factor 1 revealsa regulatory role for oligomerization in DNA-binding specificity (2010)
  • Author(s): Enoki, Y.
  • Journal Title: Biochimica et Biophysica Acta, Molecular Basis of Disease Volume: 1802 Pages: 749-753
  • Peer Reviewed
• [Journal Article] Mutational analysis of human heat-shock transcription factor 1 reveals a regulatory role for oligomerization in DNA-binding specificity (2009)
  • Author(s): Takemori, Y., Enoki, Y., Yamamoto, N., Fukai, Y., Adachi, K., Sakurai, H.
  • Journal Title: Biochemical Journal Volume: 424 Pages: 253-261
  • Peer Reviewed
• [Journal Article] Galactose transporters discriminate steric anomers at the cell surface in yeast (2009)
  • Author(s): Fukasawa, T., Sakurai, H., Nogi, Y., Baruffini, E.
  • Journal Title: FEMS Yeast Research Volume: 9 Pages: 723-731
  • Peer Reviewed
• [Journal Article] Mutational analysis of human heat-shock transcription factor 1 revealsa regulatory role for oligomerization in DNA-binding specificity (2009)
  • Author(s): Takemori, Y., et al.
  • Journal Title: Biochemical Journal 424 Pages: 253-261
  • Peer Reviewed
• [Journal Article] Galactose transporters discriminate steric anomers at the cell surface in yeast (2009)
  • Author(s): Fukasawa, T., et al.
  • Journal Title: FEMS Yeast Research Pages: 723-731
  • Peer Reviewed
• [Presentation] 酵母Hsf1によるシャペロン遺伝子の発現調節:細胞増殖、ストレス抵抗性、寿命における重要性 (2011)
  • Author(s): 桜井博,太田あづ美
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-15
• [Presentation] 酵母Hsf1によるシャペロン遺伝子の発現調節 (2011)
  • Author(s): 桜井博
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-15
• [Presentation] 熱ショック転写因子HSF1のDNA結合調節 (2010)
  • Author(s): 榎康明,桜井博
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-08
• [Presentation] 熱ショック転写因子HSFIのDNA結合調節 (2010)
  • Author(s): 榎康明
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-08
• [Presentation] さまざまな生物の熱ショック転写因子の機能解析 (2009)
  • Author(s): 榎康明,向田裕香,古谷智慧,桜井博
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-12
• [Presentation] さまざまな生物の熱ショック転写因子の機能解析 (2009)
  • Author(s): 榎康明, ら
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜 (神奈川県)
  • Year and Date: 2009-12-12