Role of replication rerated nucleosome assembly factors in DNA damage repair

• Project/Area Number: 21570183
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: University of Miyazaki
• Principal Investigator: TAKAMI Yasunari 宮崎大学, 医学部, 准教授 (80236356)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: ヒストン / クロマチン / ヌクレオソーム / ジーンノックアウト法 / エピジェネティクス

Research Abstract

In eukaryotic cells, DNA replication and repair is intimately connected to chromatin structure. To understand the role of chromatin rerated factors in DNA replication and repair, we generated systematically a number of chicken DT40 mutant cell lines, respectively, lacking each of appropriate genes, CAF-1, which is consisted of p150, p60 and p48 subunit, and ASF1. By analyses of these mutant cells, we have revealed the function of CAF-1 and ASF1-dependent nucleosome assembly in DNA replication, and the relation to DNA damage repair

Research Products

• [Journal Article] GCN5 regulates the activation of PI3K/Akt survival pathway in B cells exposed to oxidative stress via controlling gene expressions of Syk and Btk (2011)
  • Author(s): Kikuchi, H., Kuribayashi, F., Takami, Y., Imajoh-Ohmi, S. and Nakayama, T.
  • Journal Title: Biophys. Res. Commun Volume: 405 Pages: 657-661
  • Peer Reviewed
• [Journal Article] GCN5 regulates the superoxide-generating system in Leukocytes via controlling gp91-phox gene expression (2011)
  • Author(s): Kikuchi, H., Kuribayashi, F., Kiwaki, N., Takami, Y. and Nakayama, T.
  • Journal Title: J. Immunology Volume: 186 Pages: 3015-3022
  • Peer Reviewed
• [Journal Article] The histo ne chaperone facilitates chromatin transcription(FACT) protein maintains normal replication fork rates (2011)
  • Author(s): Abe, T., Sugimura, K., Hosono, Y., Takami, Y., Akita, M., Yoshimura, A., Tada, S., Nakayama, T., Murofushi, H., Okumura, K., Takeda, S., Horikoshi, M., Seki, M., Enomoto, T
  • Journal Title: J. Biol. Chem Volume: 286 Pages: 30504-30512
  • Peer Reviewed
• [Journal Article] The histone chaperone facilitates chromatin transcription (FACT) protein maintains normal replication fork rates (2011)
  • Author(s): Abe T, Sugimura K, et.al.
  • Journal Title: J.Biol.Chem. Volume: 286 Pages: 30504-30512
  • Peer Reviewed
• [Journal Article] GCN5 regulates the activation of PI3K/Akt survival pathway in B cells exposed to oxdative stress via controlling gene expressions of Syk and Btk (2011)
  • Author(s): Kikuchi H., Kuribayashi F., et al
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 405 Pages: 657-661
  • Peer Reviewed
• [Journal Article] GCN5 regulates the superoxide-generating system in leukocytes via controlling gp91-phox gene expression (2011)
  • Author(s): Kikuchi H
  • Journal Title: J Immunol. Volume: 186 Pages: 3015-3022
  • Peer Reviewed
• [Journal Article] GCN5 regulates the activation of PI3K/Akt survival pathway in B cells exposed to oxidative stress via controlling gene expressions of Syk and Btk (2011)
  • Author(s): Kikuchi H
  • Journal Title: Biochem Biophys Res Commun. Volume: 405 Pages: 657-661
  • Peer Reviewed
• [Journal Article] Improved applications of the tetracycline-regulated gene depletion system (2010)
  • Author(s): Nishijima, H., Takami, Y., Nakayama, T., Adachi, N. and Shibahara, K.
  • Journal Title: Bio Science Trends Volume: 3 Pages: 161-167
• [Journal Article] Histone H1 null vertebrate cells exhibit altered nucleosome architecture (2010)
  • Author(s): Hashimoto, H., Takami, Y., Sonoda, E., Iwasaki, T., Iwano, H., Tachibana, M., Takeda, S., Nakayama, T., Kimura, H. and Shinkai, Y.
  • Journal Title: Nucleic Acids Res Volume: 38 Pages: 3533-3545
  • Peer Reviewed
• [Journal Article] Histone H1 null vertebrate cells exhibit altered nucleosome architecture (2010)
  • Author(s): Hashimoto H.
  • Journal Title: Nucleic Acids Res. Volume: 38 Pages: 3533-3545
  • Peer Reviewed
• [Journal Article] Improved applications of the tetracycline-regulated gene depletion system (2009)
  • Author(s): Nishijima H, Takami Y, Nakayama T, Adachi N, Shibahara K.
  • Journal Title: Biosci.Trends 5 Pages: 161-167
  • Peer Reviewed
• [Journal Article] クロマチン構築を介したDNA複製制御 (2009)
  • Author(s): 高見恭成
  • Journal Title: 蛋白質核酸酵素増刊号「染色体サイクル」 54 Pages: 496-501
• [Presentation] GCN5によるB細胞酸化ストレス耐性機構の解析 (2011)
  • Author(s): 菊池秀彦, 栗林太, 高見恭成, 大海忍, 中山建男
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-24
• [Presentation] GCN5によるB細胞酸化ストレス耐性機構の解析 (2011)
  • Author(s): 菊池秀彦, ら
  • Organizer: 日本生化学会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-24
• [Presentation] ニワトリDT40細胞を用いたミトコンドリアSirtuinsの機能解析 (2010)
  • Author(s): 鈴木寛之、中山建男、高見恭成
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2010-06-17
• [Presentation] Histone chaperone FACT is involved in efficient elongation of nascent DNA chain (2009)
  • Author(s): 阿部拓也、大沢洸司、高見恭成、多田周右、中山建男、室伏廣、堀越正美、関政幸、榎本武美
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] ノックイン変異細胞を用いたASF1/CIAの機能解析 (2009)
  • Author(s): 高見恭成、中山建男
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] ノックイン変異細胞を用いたASF1/CIAの機能解析 (2009)
  • Author(s): 高見恭成, 中山建男
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] ノックイン変異ASF1/CIA細胞を用いたASF1の機能解析 (2009)
  • Author(s): 高見恭成、中山建男
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Book] Studies on epigenetic control of B cell functions using the DT40 cell line. Advances in Genetics Research Vol.2, Nova Science Publishers (2010)
  • Author(s): Kikuchi, H., Barman, H. K., Nakayama, M., Takami, Y. and Nakayama, T.
  • Publisher: Inc. NY
• [Remarks]
  • URL: http://www.med.miyazaki-u.ac.jp/2bio/index.html