Regulatory mechanism for intracellular transport depending on the DLK-JNK signaling pathway

• Project/Area Number: 21570184
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Wakayama Medical University (2012); Yokohama City University (2009-2011)
• Principal Investigator: HIRAI Syu-ichi 和歌山県立医科大学, 医学部, 教授 (80228759)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: JNK / JIP / Kinesin-1 / 細胞内輸送 / 微小管 / 神経軸索 / Kinesin / キネシン

Research Abstract

Intracellular trafficking is one of crucial factors supporting cell differentiation and function. This depends on motor proteins, which carry different kinds of cargos, and cytoskeletons, which serve as scaffold or rail of motor proteins. We showed that a protein kinase DLK affect the intracellular trafficking via two different pathways. One is the regulation of microtubules via the activation of JNK, and the other is the regulation of the binding between kinesin motor and cargo molecules via adaptor protein JIP. These findings give new insight into the molecular mechanism regulating intracellular trafficking.

Research Products

• [Journal Article] the interaction of Kinesin-1 with its adaptor protein JIP1 can be regulated via proteins binding to the JIP1-PTB domain (2013)
  • Author(s): T.Satake,K.Ohtsuki,Y.Banba,J.Suenaga,H.Hirano,Y.Yamanaka,S.Ohno,S.Hirai
  • Journal Title: BMC Cell Biol Volume: 14 Pages: 12-12
  • Peer Reviewed
• [Journal Article] Functional genomic screening identifies dual leucine zipper kinase as a key (2013)
  • Author(s): D.S.Welsbie,Z.Yang,Y.Ge,K.L.Mitchell,X.Zhou,S.E.Martin,C.A.Berlinicke,L.HacklerJr,J.Fuller,J.Fu,L.H.Cao,B.Han,D.Auld,T.Xue,S.Hirai,L.Germain,C.SimardBisson,R.Blouin,J.V.Nguyen,C.H.Davis,R.A.Enke,S.L.Boye,S.L.Merbs,N.Marsh-Armstrong,W.W.Hauswirth,A.Diantonio,R.W.Nickells,J.Inglese,J.Hanes,K.W.Yau,H.A.Quigley,D.J.Zack
  • Journal Title: mediator of retinal ganglion cell death Volume: 110 Pages: 4045-4050
  • Peer Reviewed
• [Journal Article] The interaction of Kinesin-1 with its adaptor protein JIP1 can be regulated via proteins binding to the JIP1-PTB domain. (2013)
  • Author(s): Satake T, et al.
  • Journal Title: BMC Cell Biol Volume: 14 Issue: 1 Pages: 12-12
  • DOI: 10.1186/1471-2121-14-12
  • Peer Reviewed
• [Journal Article] Functional genomic screening identifies dual leucine zipper kinase as a key mediator of retinal ganglion cell death. (2013)
  • Author(s): Welsbie D, et al.
  • Journal Title: Proc Natl Acad Sci Volume: 110 Issue: 10 Pages: 4045-4050
  • DOI: 10.1073/pnas.1211284110
  • Peer Reviewed
• [Journal Article] 軸索形成に必要なDLK-JNK経路と微小管制御 (2012)
  • Author(s): 平井秀一
  • Journal Title: 生体の科学 Volume: 63 Pages: 183-188
  • Peer Reviewed
• [Journal Article] Axon formation in neocortical neurons depends on stage-specific regulation of microtubule stability by the Dual Leucine zipper Kinase-c-Jun N-terminal Kinase pathway (2011)
  • Author(s): S.Hirai,Y.Banba,T.Satake,S.Ohno
  • Journal Title: J.Neurosci Volume: 31 Pages: 6468-6480
  • Peer Reviewed
• [Journal Article] Maintenance of dendritic spine morphology by partitioning-defective 1b through regulation of microtubule growth (2011)
  • Author(s): K.Hayashi,A.Suzuki,S.Hirai,Y.Kurihara,C.C.Hoogenraad,S.
  • Journal Title: J。Neurosci Volume: 31 Pages: 12094-12103
  • Peer Reviewed
• [Journal Article] Maintenance of dendritic spine morphology by partitioning-defective 1b through regulation of microtubule growth (2011)
  • Author(s): Hayashi K, Suzuki A, Hirai S, Kurihara Y, Hoogenraad CC, Ohno S
  • Journal Title: J. Neurosci. Volume: 31 Issue: 34 Pages: 12094-12103
  • DOI: 10.1523/jneurosci.0751-11.2011
  • Peer Reviewed
• [Journal Article] Axon Formation in Neocortical Neurons Depends on Stage-Specific Regulation of Microtubule Stability by the Dual Leucine Zipper Kinase-c-Jun N-Terminal Kinase Pathway. (2011)
  • Author(s): Syu-ichi Hirai, et al.
  • Journal Title: J.Neurosci. Volume: 31 Pages: 6468-6480
  • Peer Reviewed
• [Journal Article] JNK phosphorylates Ser332 of doublecortin and regulates its function in neurite extension and neuronal migration (2010)
  • Author(s): J.Jin,H.Suzuki,S.Hirai,K.Mikoshiba,T.Ohshima
  • Journal Title: Dev.Neurobiol Volume: 70 Pages: 929-942
  • Peer Reviewed
• [Journal Article] Dominant-negative mutations in a-II spectrin cause West syndrome with severe cerebral hypomyelination (2010)
  • Author(s): H.Saitsu,J.Tohyama,T.Kumada,K.Egawa,K.Hamada,I.Okada,T.Mizuguchi,H.Osaka,shR.Miyata,T.Furukawa,K.Haginoya,H.Hoshino,T.Goto,Y.Hachiya,T.Yamagata,S.Saitoh,T.Nagai,K.Niiyama,A.Nishimura,N.Miyake.M.Komada,K.Hayashi,S.Hirai,K.Ogata,M.Kato,A.Fukuda,N.Matsumoto
  • Journal Title: spastic quadriplegia, and developmental delay Volume: 86 Pages: 1-11
  • Peer Reviewed
• [Journal Article] がん細胞を知る-分子レベルでみた正常細胞との違い- (2010)
  • Author(s): 平井秀一
  • Journal Title: Medical Technology Volume: 38 Pages: 503-506
• [Journal Article] JNK phosphorylates Ser332 of doublecortin and regulates its function inneurite extension and neuronal migration. (2010)
  • Author(s): Junghee Jin, et al.
  • Journal Title: Dev.Neurobiol. Volume: 79 Pages: 929-942
  • Peer Reviewed
• [Journal Article] Dominant-negative mutations in a-II spectrin cause West syndrome with severe cerebral hypomyelination, spastic quadriplegia, and developmental delay. (2010)
  • Author(s): Hirotomo Saitsu, et al.
  • Journal Title: Am.J.Hum.Genet. Volume: 86 Pages: 1-11
  • Peer Reviewed
• [Journal Article] がん細胞を知る -分子レベルで見た正常細胞との違い- (2010)
  • Author(s): 平井秀一
  • Journal Title: Medical Technology 38 Pages: 503-506
• [Presentation] JNK reduces SCG10 protein level to regulate axon formation (2012)
  • Author(s): Hirai S,Ohno
  • Organizer: 2012 Annual Meeting of The American Society for Cell Biology
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2012-12-19
• [Presentation] JNK facilitates the formation of epithelial cell sheet (2012)
  • Author(s): Furukawa K, Ohno S, Hirai S
  • Organizer: 第33回日本分子生物学会年会、第83回日本生化学会合同大会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] JNK reduces SCG10 protein level to regulate axon formation. (2012)
  • Author(s): Hirai S, Ohno S
  • Organizer: 2012 Annual Meeting of The American Society for Cell Biology
  • Place of Presentation: San Francisco, USA
• [Presentation] Molecular mechanism supporting transitions in cell polarity during neuronal development (2011)
  • Author(s): Hirai S
  • Organizer: The 34thAnnual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Yokohama
  • Year and Date: 2011-12-15
• [Presentation] Molecular mechanism supporting transitions in cell polarity during Neuronal development (2011)
  • Author(s): Syu-ichi Hirai
  • Organizer: The 34^<th> Annual Meeting of the Molecular Biology Society of Japan (3S3pI-5)
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-15
• [Presentation] Axon formation in neocortical neurons depends on the stage-specific stabilization of microtubules by DLK-JNK pathway (2010)
  • Author(s): Hirai S, Banba Y, Satake T, Ohno S
  • Organizer: 第33回日本分子生物学会年会,第83回日本生化学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] egulatory mechanism for Kinesin-1 motor activity through JIP1 (2010)
  • Author(s): Satake T, Banba Y, Ohno S, Hirai S
  • Organizer: 第33回日本分子生物学会年会、第83回日本生化学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Axon formation in neocortical neurons depends on the stage-specific stabilization of microtubules by DLK-JNK pathway. (2010)
  • Author(s): Syu-ichi Hirai, et.al.
  • Organizer: 第33回日本分子生物学会年会、第83回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫)
  • Year and Date: 2010-12-08
• [Presentation] Regulatory mechanism for Kinesin-1 motor activity through JIP1. (2010)
  • Author(s): Tomoko Satake, et.al.
  • Organizer: 第33回日本分子生物学会年会、第83回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫)
  • Year and Date: 2010-12-08
• [Presentation] egulatory mechanism for Kinesin-1 motor activity through JIP1 (2009)
  • Author(s): Satake T, Banba Y, Ohno S, Hirai S
  • Organizer: 第31回日本分子生物学会年会、第81回日本生化学会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] Regulatory mechanism for Kinesin-1 motor activity through JIP1 (2009)
  • Author(s): Tomoko Satake, 他3名
  • Organizer: 第31回日本分子生物学会年会、第81回日本生化学会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] JNK as a coordinator of microtubule modulation facilitating axon formation (2009)
  • Author(s): Syu-ichi Hirai, 他3名
  • Organizer: 第31回日本分子生物学会年会、第81回日本生化学会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Remarks]
  • URL: http://www.wakayama-med.ac.jp/med/lasbi
• [Remarks]
  • URL: http://ology1/Biology_Index.html
• [Remarks] 和歌山県立医科大学医学部教養医学教育大講座生物学教室
  • URL: http://www.wakayama-med.ac.jp/med/lasbiology1/Biology_Index.html
• [Remarks]
  • URL: http://www-user.yokohama-cu.ac.jp/~ohnos/research/dlk.html
• [Remarks]
  • URL: http://www-user.yokohama-cu.ac.jp/~ohnos/research/dlk.html
• [Remarks]
  • URL: http://www-user.yokohama-cu.ac.jp/~ohnos//research/dlk.html