Functional elucidation of IRBIT, a master regulator in the central nervous system

• Project/Area Number: 21570190
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Showa Pharmaceutical University (2011); The Institute of Physical and Chemical Research (2009-2010)
• Principal Investigator: MIZUTANI Akihiro 昭和薬科大学, 薬学部, 教授 (30242861)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: IRBIT / 多重リン酸化領域 / 細胞外環境応答 / ストレス反応 / 多重リン酸化 / 細胞外環境センサー / IP3レセプター / マスター調節因子

Research Abstract

IRBIT is highly expressed in the glial processes facing and/or ensheathing synaptic clefts. IRBIT interacts with both IP3 receptor and NBC1 in its phosphorylation dependent manner, and modulates their functions. The phosphorylated residues on IRBIT which binds to IP3R with high affinity were revealed to differ from those of IRBIT which binds to NBC1.

Research Products

• [Journal Article] Syntaxin 1B Suppresses Macropinocytosis and Semaphorin 3A-Induced Growth Cone Collapse (2011)
  • Author(s): Kabayama H
  • Journal Title: J Neurosci Volume: 31(20) Issue: 20 Pages: 7357-64
  • DOI: 10.1523/jneurosci.2718-10.2011
  • Peer Reviewed
• [Journal Article] IRBIT governs epithelial secretion in mice by antagonizing the WNK/SPAK kinase pathway (2011)
  • Author(s): Yang D
  • Journal Title: J Clin Invest Volume: 121(3) Issue: 3 Pages: 956-65
  • DOI: 10.1172/jci43475
  • Peer Reviewed
• [Journal Article] Syntaxin 1B suppresses macropinocytosis and semaphorin 3A-induced growth cone collapse (2011)
  • Author(s): 樺山博之
  • Journal Title: J.Neurosci. Volume: 31 Pages: 7357-7364
  • Peer Reviewed
• [Journal Article] IRBIT governs epithelial secretion in mice by antagonizing the WNK/SPAK kinase pathway. (2011)
  • Author(s): Yang D, Li Q, So I, Huang CL, Ando H, Mizutani A, Seki G, Mikoshiba K, Thomas PJ, Muallem S.
  • Journal Title: J Clin Invest Volume: (In press)
  • Peer Reviewed
• [Journal Article] Isolation of inositol 1, 4, 5-trisphosphate receptor-associating proteins and selective knockdown using RNA interference (2010)
  • Author(s): Mizutani A, Kawaai K, Hisatsune C, Ando H, Michikawa T, Mikoshiba K
  • Journal Title: Methods Mol Biol Volume: 645 Pages: 133-41
  • DOI: 10.1007/978-1-60327-175-2_9
  • ISBN: 9781603271745, 9781603271752
• [Journal Article] Isolation of inositol 1,4,5-trisphosphate receptor-associating proteins and selective knockdown using RNA interference. (2010)
  • Author(s): Mizutani A, Kawaai K, Hisatsune C, Ando H, Michikawa T, Mikoshiba K
  • Journal Title: Methods Mol Biol Volume: 645 Pages: 133-141
• [Journal Article] Inositol 1, 4, 5-triphosphate receptor-binding protein released with Inositol 1, 4, 5-triphosphate(IRBIT), associates with components of the mRNA 3' processing machinery in a phosphorylation-dependent manner and inhibits polyadenylation (2009)
  • Author(s): Kiefer H, Mizutani A, Iemura A, Natsume T, Ando H, Kuroda Y, Mikoshiba K
  • Journal Title: J.Biol.Chem. 284 Pages: 10694-10705
  • Peer Reviewed
• [Journal Article] An IRBIT homologue lacks binding activity to inositol 1, 4, 5-trisphosphate receptor due to the unique N-terminal appendage (2009)
  • Author(s): Ando H, Mizutani A, Mikoshiba K.
  • Journal Title: J.Neurochem 109 Pages: 539-550
  • Peer Reviewed
• [Journal Article] IRBIT coordinates epithelial fluid and HCO3- secretion by stimulating the transporters pNBC1 and CFTR in the murine pancreatic duct (2009)
  • Author(s): Yang D, Shcheynikov N, Zeng W, Ohana E, So I, Ando H, Mizutani A, Mikoshiba K, Muallem S
  • Journal Title: J.Clin.Invest. 119 Pages: 193-202
  • Peer Reviewed
• [Presentation] IRBITの多重リン酸化領域におけるリン酸化パターンによって、IRBITの標的分子との相互作用が規定される (2011)
  • Author(s): 水谷顕洋、御子柴克彦
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2011-09-22
• [Presentation] Multi-site phosphorylation regulates the target recognition of IRBIT (2011)
  • Author(s): 水谷顕洋
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2011-09-22
• [Presentation] IRBITの中枢神経系における分布について (2009)
  • Author(s): 水谷顕洋、黒田有希子、安東英明、永吉旭土、河合克宏、御子柴克彦
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場 1号館1階 イベントホール
  • Year and Date: 2009-09-16