| Project/Area Number |
21570192
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Research Collaborator |
TANAKA Kazuma 北海道大学, 遺伝子病制御研究所, 教授 (60188290)
KAMADA Konomi 北海道大学, 遺伝子病制御研究所, 准教授 (80312354)
NAGATANI Akira 京都大学, 理学研究科, 教授 (40183082)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 生体膜 / リン脂質非対称分布 / リン脂質フリッパーゼ / 細胞内小胞輸送 / フリッパーゼキナーゼ / モデル生物 / 出芽酵母 / 遺伝学 / 脂質 / アレスチン / ArfGAP |
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| Research Abstract |
Our yeast genetic screening successfully identified Art5, one of α-arrestins, as a novel regulator of Dnf1/2 phospholipid flippases. We found that Art5 may condition phospholipid asymmetry of biomembranes, mediated by its binding with Dnf1/2. In addition, we developed a novel method to analyze functions of PHOT kinases, plant homologues of yeast Fpk1/2 flippase kinases, by producing them in the living yeast system.
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