Coupling mRNA nuclear export to transcription and translation

• Project/Area Number: 21570195
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: KATAHIRA Jun 大阪大学, 生命機能研究科, 准教授 (30263312)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 遺伝子発現制御 / mRNA / 核-細胞質間輸送 / 翻訳制御 / 熱ショック遺伝子 / 核-細胞質問輸送 / 核-細胞質間輪送 / 転写伸長

Research Abstract

The aim of this study is to better understand the function of the human Transcription-Export(TREX) complex in gene expression. I examined intracellular localization and structural alteration of the TREX complex under normal and stressed conditions. In addition, protein factors associating with the human TREX complex and a novel role of the TREX complex in protein translation were analyzed.

Research Products

• [Journal Article] mRNA export and the TREX complex (2012)
  • Author(s): Katahira, J.
  • Journal Title: Biochim. Biophys. Acta.-Gene regulatory mechanisms Volume: 1891 Pages: 507-513
  • Peer Reviewed
• [Journal Article] Nucleo-cytoplasmic transport of microRNAs and related small RNAs (2011)
  • Author(s): Katahira, J. and Yoneda, Y.
  • Journal Title: Traffic Volume: 12 Pages: 1468-1474
  • Peer Reviewed
• [Journal Article] Cell type-dependent gene regulation by Staufen2 in conjunction with Upf1 (2011)
  • Author(s): Miki, T., Kamikawa, Y., Kurono, S., Kaneko, Y., Katahira, J., Yoneda, Y.
  • Journal Title: BMC Mol. Biol Volume: 12 Pages: 48-48
  • Peer Reviewed
• [Journal Article] Cell type-dependent gene regulation by Staufen2in conjunction with Upf1 (2011)
  • Author(s): Miki, T., Kurono, S., Kaneko, Y., Katahira, J. and Yoneda, Y
  • Journal Title: BMC Mol. Biol. Volume: 12 Issue: 1 Pages: 48-48
  • DOI: 10.1186/1471-2199-12-48
  • Peer Reviewed
• [Journal Article] mRNAの核外輸送と細胞内局在化機構 (2011)
  • Author(s): 片平じゅん, ら
  • Journal Title: 生体の科学 Volume: 62 Pages: 69-74
  • NAID: 40018713434
• [Journal Article] Identification of importin alphal as a novel constituent of RNA stressgranules. (2010)
  • Author(s): K.Fujimura, et al.
  • Journal Title: Biochim.Biophys.Acta. Volume: 1803 Pages: 865-871
  • Peer Reviewed
• [Journal Article] Roles of the TREX complex in nuclear export of mRNA (2009)
  • Author(s): Katahira, J. and Yoneda, Y.
  • Journal Title: RNA Biol Volume: 6 Pages: 149-152
  • Peer Reviewed
• [Journal Article] 共立出版 (2009)
  • Author(s): 片平じゅん
  • Journal Title: 蛋白質核酸酵素 増刊・mRNAプログラム-多様性と非対称性の獲得戦略 54 Pages: 2109-2113
• [Journal Article] Roles of the TREX complex in nuclear export of mRNA (2009)
  • Author(s): 片平じゅん., et al.
  • Journal Title: RNA Biology 6 Pages: 149-152
  • Peer Reviewed
• [Journal Article] A high-resolution structure of the pre-microRNA nuclear export machinery (2009)
  • Author(s): Okada, C., et al.
  • Journal Title: Science 326 Pages: 1275-1279
  • Peer Reviewed
• [Journal Article] Selective localization of PCBP2 to cytoplasmic processing bodies (2009)
  • Author(s): Fujimura K., et al.
  • Journal Title: BBA 1793 Pages: 878-887
  • Peer Reviewed
• [Journal Article] Microscopic dissection of the process of stress granule assembly (2009)
  • Author(s): Fujimura K., et al.
  • Journal Title: BBA 1793 Pages: 1728-1737
  • Peer Reviewed
• [Presentation] Roles of the human TREX complex in gene expression (2009)
  • Author(s): 片平じゅん
  • Organizer: 2009 International meeting on nuclear trafficking
  • Place of Presentation: Banff, Alberta, Canada
  • Year and Date: 2009-08-28
• [Presentation] Roles of the human TREX complex in gene expression(一般口頭発表) (2009)
  • Organizer: 2009 International meeting on nuclear trafficking
  • Place of Presentation: Banff、Alberta、Canada
  • Year and Date: 2009-08-24
• [Presentation] Thoc5およびAly/ REFはHSP70 mRNA核外輸送のアダプターとして機能する(一般口頭発表) (2009)
  • Organizer: 第11回RNAミーティング
  • Place of Presentation: 新潟
  • Year and Date: 2009-07-27
• [Presentation] Thoc5 およびAly/REFはHSP70 mRNA核外輸送のアダプターとして機能する (2009)
  • Author(s): 片平じゅん
  • Organizer: 第11回 日本RNA学会総会
  • Place of Presentation: 朱鷺メッセ (新潟)
  • Year and Date: 2009-07-27
• [Presentation] Adaptor Aly and Co-Adaptor Thoc5 Function in the Tap-p15-Mediated Nuclear Export of HSP70 mRNA (2009)
  • Author(s): 片平じゅん
  • Organizer: 第61回 日本細胞生物学会
  • Place of Presentation: 名古屋国際会議場 (愛知)
  • Year and Date: 2009-06-27
• [Presentation] Adaptor Aly and Co-Adaptor Thoc5 Function in the Tap-p15-Mediated Nuclear Export of HSP70 mRNA(ポスター発表) (2009)
  • Organizer: 第61回細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-02
• [Book] mRNAの核外輸送と細胞内局在化機構、生体の科学 (2011)
  • Author(s): 片平じゅん、米田悦啓
• [Remarks]
  • URL: http://www.anat3.med.osaka-u.ac.jp/research/research3_1.html