Genome side screening of autophagy to elucidate its regulation

• Project/Area Number: 21570197
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: NODA Takeshi 大阪大学, 生命機能研究科, 准教授 (00290908)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: オートファジー / 酵母 / スクリーニング / ゲノムワイドスクリーン / タンパク質分解 / Tor

Research Abstract

Autophagy is intracellular bulk degradation system, but its regulatory mechanism is largely unknown. In this study, we succeeded in introducing ALP system, which enables quantitation of autophagy activity, into the genome wide yeast mutant collection. We measured autophagic activity under nitrogen starvation in large scale and found that several intriguing factors are directly associated with autophagy regulation. Thus we advanced understanding the mechanism of autophagy regulation.

Research Products

• [Journal Article] Dysfunction of autophagy participates in vacuole formation and cell death in cells replicating hepatitis C virus (2011)
  • Author(s): Taguwa, S., Kambara, H., Fujita, N., Noda, T., Yoshimori, T., Koike, K., Moriishi, K., Matsuura, Y.*
  • Journal Title: J Virol
  • URL: http://jvi.asm.org/content/early/2011/10/12/JVI.06099-11.long.
  • Peer Reviewed
• [Journal Article] The LC3 recruitment mechanism is separate from Atg9L1 dependent membrane formation in the autophagic response against Salmonella (2011)
  • Author(s): Kageyama, S., Omori, H., Saitoh, T., Sone, T., Guan, J. L., Akira, S., Imamoto, F., Noda, T.*, Yoshimori, T.*
  • Journal Title: Mol Biol Cell Volume: 22 Pages: 2290-2300
  • Peer Reviewed
• [Journal Article] Atg14L recruits PtdIns 3-kinase to the ER for autophagosome formation (2011)
  • Author(s): Noda, T.*, Matsunaga, K. & Yoshimori, T.*
  • Journal Title: Autophagy Volume: 7 Pages: 438-439
  • Peer Reviewed
• [Journal Article] Chemical modulators of autophagy as biological probes and potential therapeutics (2011)
  • Author(s): Fleming, A., Noda, T., Yoshimori, T.* & Rubinsztein, D. C.*
  • Journal Title: Nat Chem Biol Volume: 7 Pages: 9-17
  • Peer Reviewed
• [Journal Article] Modulation of local PtdIns3P levels by the PI phosphatase MTMR3 regulates constitutive autophagy (2010)
  • Author(s): Taguchi-Atarashi, N., Hamasaki, M., Matsunaga, K., Omori, H., Ktistakis, N. T., Yoshimori, T., Noda, T.*
  • Journal Title: Traffic Volume: 11 Pages: 468-478
  • Peer Reviewed
• [Journal Article] Rubicon and PLEKHM1 negatively regulate the endocytic/ autophagic pathway via a novel Rab7-binding domain (2010)
  • Author(s): Tabata, K., Matsunaga, K., Sakane, A., Sasaki, T., Noda, T., Yoshimori, T.*
  • Journal Title: Mol Biol Cell Volume: 21 Pages: 4162-4172
  • Peer Reviewed
• [Journal Article] Autophagy requires endoplasmic reticulum targeting of the PI3-kinase complex via Atg14L (2010)
  • Author(s): Matsunaga, K., Morita, E., Saitoh, T., Akira, S., Ktistakis, N. T., Izumi, T., Noda, T.*, Yoshimori, T.*
  • Journal Title: J Cell Biol Volume: 190 Pages: 511-521
  • Peer Reviewed
• [Journal Article] Combinational soluble N-ethylmaleimide-sensitive factor attachment protein receptor proteins VAMP8 and Vti1b mediate fusion of antimicrobial and canonical autophagosomes with lysosomes (2010)
  • Author(s): Furuta, N., Fujita, N., Noda, T., Yoshimori, T., Amano, A.*
  • Journal Title: Mol Biol Cell Volume: 21 Pages: 1001-1010
  • Peer Reviewed
• [Journal Article] Between canonical and antibacterial autophagy : Rab7 is required for GAS-containing autophagosome-like vacuole formation (2010)
  • Author(s): Noda, T. & Yoshimori, T.*
  • Journal Title: Autophagy Volume: 6 Pages: 419-420
  • Peer Reviewed
• [Journal Article] Regulation of membrane biogenesis in autophagy via PI3P dynamics (2010)
  • Author(s): Noda, T.*, Matsunaga, K., Taguchi-Atarashi, N. & Yoshimori, T.*
  • Journal Title: Semin Cell Dev Biol Volume: 21 Pages: 2010-2010
  • Peer Reviewed
• [Journal Article] Electron tomography reveals the endoplasmic reticulum as a membrane source for autophagosome formation (2010)
  • Author(s): Hayashi-Nishino, M., Fujita, N., Noda, T., Yamaguchi, A., Yoshimori, T. & Yamamoto, A.*
  • Journal Title: Autophagy Volume: 6 Pages: 301-303
  • Peer Reviewed
• [Journal Article] Modulation of Local PtdIns3P Levels by the PI Phosphatase MTMR3 Regulates Constitutive Autophagy (2010)
  • Author(s): Naoko Taguchi-Atarashi, Maho Hamasaki, Kohichi Matsunaga, Hiroko Omori, Nicholas T Ktistakis, Tamotsu Yoshimori, Takeshi Noda
  • Journal Title: Traffic Volume: 11 Pages: 468-478
  • Peer Reviewed
• [Journal Article] Combinational SNARE Proteins VAMP8 and Vti1b Mediate Fusion of Antimicrobial and Canonical Autophagosomes with Lysosomes (2010)
  • Author(s): Nobumichi Furuta, Naonobu Fujita, Takeshi Noda, Tamotsu Yoshimori, Atsuo Amano
  • Journal Title: Mol Biol Cell Volume: 21 Pages: 1001-1010
  • Peer Reviewed
• [Journal Article] Modulation of local PtdIns3P levels by the PI phosphatase MTMR3 regulates constitutive autophagy (2010)
  • Author(s): N Taguchi-Atarashi, M Hamasaki, K Matsunaga, H Omori, NT Ktistakis, T Yoshimori, T Noda
  • Journal Title: Traffic 4 Pages: 468-478
  • Peer Reviewed
• [Journal Article] Two Beclin 1-binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages (2009)
  • Author(s): K Matsunaga, T Saitoh, K Tabata, H Omori, T Satoh, N Kurotori, I Maejima, K Shirahama-Noda, T Ichimura, T Isobe, S Akira, T Noda, T Yoshimori
  • Journal Title: Nat Cell Biol 11 Pages: 385-396
  • Peer Reviewed
• [Journal Article] Differential involvement of Atg16L1 in Crohn disease and canonical autophagy : analysis of the organization of the Atg16L1 complex in fibroblasts (2009)
  • Author(s): N Fujita, T Saitoh, S Kageyama, S Akira, T Noda, T Yoshimori
  • Journal Title: J Biol Chem 284 Pages: 32602-32609
  • Peer Reviewed
• [Presentation] 酵母研究が切り開く細胞内自己分解のありかた (2011)
  • Author(s): 野田健司
  • Organizer: 大阪大学寄附講座酵母リソース工学開設式記念講演会
  • Place of Presentation: 吹田
  • Year and Date: 2011-11-25
• [Presentation] Critical role of PtdIns3P turn over in autophagy regulation (2011)
  • Author(s): Noda, T., Taguchi-Atarashi, N., Yoshimori, T.
  • Organizer: International Symposium New Aspects of Phospholipid Biology and Medicine 2011, New Aspects of Phospholipid Biology and Medicine 2011
  • Place of Presentation: 博多
  • Year and Date: 2011-11-15
• [Presentation] オートファジーによるサルモネラ増殖抑制機構 (2011)
  • Author(s): 野田健司
  • Organizer: 第2回加藤記念研究助成成果報告交流会
  • Place of Presentation: 町田
  • Year and Date: 2011-11-07
• [Presentation] オートファジーに於ける定常的小胞輸送の関与 (2011)
  • Author(s): 野田佳苗, 吉良新太郎, 吉森保, 野田健司
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会館
  • Year and Date: 2011-09-24
• [Presentation] How is the Membrane Biogenesis Regulated in Autophagy? (2010)
  • Author(s): 野田健司
  • Organizer: 日本生化学会
  • Place of Presentation: 神戸ポートピアホテル(招待講演)
  • Year and Date: 2010-12-10
• [Presentation] How is TRAPPIII involved in the yeast autophagy? (2010)
  • Author(s): Noda, T.
  • Organizer: The 2nd Japan-Sino Autophagy Conference
  • Place of Presentation: 葉山
  • Year and Date: 2010-12-09
• [Presentation] How is the Membrane Biogenesis Regulated in Autophagy? (2010)
  • Author(s): Noda, T.
  • Organizer: 日本生化学会日本分子生物学会合同年会シンポジウムRecent Trends in Membrane Traffic
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Elucidation of autophagy regulation mechanism through comprehensive yeast mutant screening (2010)
  • Author(s): Noda, T.
  • Organizer: The 1st Shino-Japan autophagy conference
  • Place of Presentation: Xi' an, China
  • Year and Date: 2010-10-06
• [Presentation] Autophagy requires endoplasmic reticulum-targeting of the PI3-kinase complex via Atg14L (2010)
  • Author(s): Matsunaga, K., , Morita, E., Saitoh, T., Akira, S., Ktistakis N. T., Izumi, T., Noda, T., Yoshimori, T
  • Organizer: EMBO Conference, Towards a comprehensive understanding of endoplasmic reticulum functions
  • Place of Presentation: Girona, Spain
  • Year and Date: 2010-10-04
• [Presentation] オートファジーの膜動態制御機構 (2010)
  • Author(s): 野田健司
  • Organizer: 大阪大学蛋白質研究所セミナー疾患と膜動態の蛋白質科学
  • Place of Presentation: 吹田
  • Year and Date: 2010-09-18
• [Presentation] Recruitment of LC3 is separable from the membrane generation system in autophagy against Salmonella. (2010)
  • Author(s): Kageyama, S., Omori, H., Saitoh, T., Sone, T., Akira S., Imamoto, F., Noda, T., Yoshimori, T.
  • Organizer: The 10^<th> Awaji International Forum on Infection and Immunity(Poster pick up)
  • Place of Presentation: 淡路島
  • Year and Date: 2010-09-09
• [Presentation] PI3Pによるオートファジーの制御機構 (2010)
  • Author(s): 野田健司
  • Organizer: 第10回日本蛋白質科学会年会ワークショップオートファジーの構造と機能
  • Place of Presentation: 札幌
  • Year and Date: 2010-06-16
• [Presentation] Genome-wide screening of autophagy related genes (2009)
  • Author(s): S Kira, T Yoshimori, T.Noda
  • Organizer: International symposium on autophagy
  • Place of Presentation: 大津プリンスホテル
  • Year and Date: 2009-09-25
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/yoshimori/jp/achievement/010/
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/yoshimori/jp/achievement/010/
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/yoshimori/jp/achievement/010/
• [Remarks]
  • URL: http://www.biken.osaka-u.ac.jp/act/act_yoshimori.php