Analysis of mechanisms to regulate oxidative stress response by glyceraldehyde-3-phosphate dehydrogenase

• Project/Area Number: 21570198
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: MORIGASAKI Susumu 奈良先端科学技術大学院大学, バイオサイエンス研究科, 研究員 (90242487)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 細胞内情報伝達 / タンパク質複合体 / target of rapamycin / グリセルアルデヒド-3-リン酸脱水素酵素 / ストレス / シグナル伝達 / ターゲットオブラパマイシン / MAPキナーゼカスケード / 分裂酵母 / Schizosaccharomyces pombe / 環境応答 / 低分子量型GTPase / ストレス応答 / Schizosaccharomyices pombe

Research Abstract

As environmental conditions are always fluctuating, it is most important function for organisms to sense the changes and protect themselves. I, in this research, biochemically analyzed two signal transduction pathways that control stress resistance or proliferation/growth. Moreover, I elucidated function of a glycolytic enzyme GAPDH on the pathways. In conclusion, GAPDH has functions as a sensor of oxidative stress and a master regulator of adaptation to the stress conditions, in addition to the classical function.

Research Products

• [Journal Article] Rab-family GTPase regulates TOR complex 2 signaling in fission yeast (2010)
  • Author(s): Hisashi Tatebe, Susumu Morigasaki, Shinichi Murayama, Cui Tracy Zeng, and Kazuhiro Shiozaki
  • Journal Title: Current Biology Volume: 20巻 Pages: 1975-1982
  • Peer Reviewed
• [Journal Article] Rab-family GTPase regulates TOR complex 2 signaling in fission yeast (2010)
  • Author(s): Hisashi Tatebe, Susumu Morigasaki, Shinichi Murayama, Cui Tracy Zeng, Kazuhiro Shiozaki(第一著者)
  • Journal Title: Gurrent Biology Volume: 20 Pages: 1975-1982
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): Susumu Morigasaki, Kazuhiro Shiozaki
  • Journal Title: Methods in Enzymology, vol.471, "Two-Component Signaling Systems, Part C" (Edit.by M.I.Simon, B.R.Crane, A.Crane)(Elsevier Inc. (Academic Press)) Pages: 279-289
• [Presentation] Studies on a TORC2 regulation mechanism in the fission yeast Schizosaccharomyces pombe (2011)
  • Author(s): 森ヶ崎進
  • Organizer: 第34回日本分子生物学会年会(MBSJ2011)
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2011-12-16
• [Presentation] 分裂酵母におけるTdh1とRyh1によるTORC2経路の制御機構の解析 (2011)
  • Author(s): 森ヶ崎進
  • Organizer: 酵母遺伝学フォーラム第44回研究報告会
  • Place of Presentation: 九州大学医学部百年講堂(福岡)
  • Year and Date: 2011-09-06
• [Presentation] Ryh1, an ortholog of mammal Rab6 small GTPase associates with and regulates the target of rapamycin complex 2 (2011)
  • Author(s): Susumu Morigasaki
  • Organizer: The sixth international fission yeast meeting(pombe 2011)
  • Place of Presentation: Martin Conference Center(Boston, USA.)
• [Presentation] Ryh1, an ortholog of mammal Rab6 small GTPase associates with and regulates the target of rapamycin complex 2 (2011)
  • Author(s): Susumu Morigasaki, Hisashi Tatebe, Cui Tracy Zeng, Tomoyuki Hatano, Hiroshi Takagi, Kaz Shiozaki
  • Organizer: The sixth international fission yeast meeting (pombe2011)
  • Place of Presentation: Martin Conference Center (Boston, U.S.A.)(複数日ポスター発表)
• [Presentation] Regulation of the target of rapamycin complex 2 by a Rab-family small G-protein in the fission yeast Schizosaccharomyces pombe (2010)
  • Author(s): 森ヶ崎進
  • Organizer: 第83回日本生化学会大会・第33回日本分子生物学会年会合同大会(BMB2010)
  • Place of Presentation: 神戸ポートアイランド(神戸)
  • Year and Date: 2010-12-10
• [Presentation] Regulation of the target of rapamycin complex 2 by a Rab-family small G-protein in the fission yeast Schizosaccharomyces pombe (2010)
  • Author(s): 森ヶ崎進、建部恒、秦野智行、塩崎一裕、高木博史
  • Organizer: 第83回日本生化学会大会・第33回日本分子生物学会年会合同大会(BMB2010)
  • Place of Presentation: 神戸ポートアイラント(兵庫県)
  • Year and Date: 2010-12-10
• [Presentation] 分裂酵母の低分子量型Gタンパク質Ryh1によるtarget of rapamycin複合体2経路の制御 (2010)
  • Author(s): 森ヶ崎進
  • Organizer: 酵母遺伝学フォーラム第43回研究報告会
  • Place of Presentation: ならまちセンター(奈良)
  • Year and Date: 2010-09-09
• [Presentation] 分裂酵母の低分子量型Gタンパク質Ryh1によるtarget of rapamycin複合体2経路の制御 (2010)
  • Author(s): 森ヶ崎進、建部恒、秦野智行、塩崎一裕、高木博史
  • Organizer: 酵母遺伝学フォーラム第43回研究報告会
  • Place of Presentation: ならまちセンター(奈良県)
  • Year and Date: 2010-09-09
• [Presentation] Wis4-Win1 MAPKKK heteromer as a platform for stress signaling (2009)
  • Author(s): Susumu Morigasaki
  • Organizer: The Fifth International Fission Yeast Meeting
  • Place of Presentation: 国立オリンピック記念青少年総合センター(東京)
  • Year and Date: 2009-10-28
• [Presentation] Wis4-Win1 MAPKKK heteromer as a platform for stress signaling (2009)
  • Author(s): Susumu Morigasaki, Aminah Ikner, Kazuhiro Shiozaki
  • Organizer: The Fifth International Fission Yeast Meeting
  • Place of Presentation: 国立オリンピック記念青少年総合センター(東京都)
  • Year and Date: 2009-10-28
• [Presentation] Glyceraldehyde-3-phosphate dehydrogenase as a modulator of the target of rapamycin complex 2 in fission yeast, Schizosaccharomyces pombe (2009)
  • Author(s): 森ヶ崎進
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場.神戸国際展示場(神戸)
  • Year and Date: 2009-10-23
• [Presentation] Glyceraldehyde-3-phosphate dehydrogenase as a modulator of the target of rapamycin complex 2 in fission yeast, Schizosaccharomyces pombe (2009)
  • Author(s): 森ヶ崎進、嶋田浩一、坂田梢、高木博史、塩崎一裕
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場・神戸国際展示場(兵庫県・神戸市)
  • Year and Date: 2009-10-23
• [Presentation] Glyceraldehyde-3-phosphate dehydrogenase mediates hydrogen peroxide-stress signaling in fission yeast, Schizosaccharomyces pombe (2009)
  • Author(s): Susumu Morigasaki
  • Organizer: 21st IUBMB and 12th FAOBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Shanghai International Conference Center(Shanghai, China)
  • Year and Date: 2009-08-05
• [Presentation] Glyceraldehyde-3-phosphate dehydrogenase mediates hydrogen peroxide-stress signaling in fission yeast, Schizosaccharomyces pombe (2009)
  • Author(s): Susumu Morigasaki, Koichi Shimada, Hiroshi Takagi, Kazuhiro Shiozaki
  • Organizer: 21^<st> IUBMB and 12^<th> FAOBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Shanghai International Conference Center (Shanghai, China)
  • Year and Date: 2009-08-05
• [Presentation] 分裂酵母Target of rapamycin複合体2を介した情報伝達系の解析 (2009)
  • Author(s): 森ヶ崎進
  • Organizer: 酵母遺伝学フォーラム第42回研究報告会
  • Place of Presentation: ノバホール(つくば)
  • Year and Date: 2009-07-30
• [Presentation] 分裂酵母Target of rapamycin複合体2を介した情報伝達系の解析 (2009)
  • Author(s): 森ヶ崎進、嶋田浩一、坂田梢、高木博史、塩崎一裕
  • Organizer: 酵母遺伝学フォーラム第42回研究報告会
  • Place of Presentation: ノバホール(茨城県・つくば市)
  • Year and Date: 2009-07-30
• [Book] Methods in Enzymology, Two-Component Signaling Systems, Part C (2010)
  • Author(s): Susumu Morigasaki and Kazuhiro Shiozaki
  • Publisher: Elsevier Inc.(Academic Press)