Mechanosensing system for regulating the cell morphology during cytokinesis

• Project/Area Number: 21570199
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Yamaguchi University
• Principal Investigator: YUMURA Shigehiko 山口大学, 大学院・医学系研究科, 教授 (70183986)
• Co-Investigator(Renkei-kenkyūsha): UYEDA Taro バイオメディシナル情報研究センター, 副研究センター長 (90356551) ; IWADATE Yoshiaki 山口大学, 大学院・医学系研究科, 准教授 (40298170)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 細胞質分裂 / アクチン / ミオシン / 細胞分裂 / 細胞性粘菌 / メカノセンシング

Research Abstract

Cells always monitor their size and shape. The excess shape change, which may result in tearing the cell membrane, should be put back by a feedback mechanism. Such mechanism may guarantee the equal division of cell volume during cytokinesis. In this study, we found actin filaments act as a mechanosensor in Dictyostelium cells. Additionally, myosin and PTEN also participated this function. The defects of this mechanism induced abnormal cytokinesis.

Research Products

• [Journal Article] Stretching actin filaments within cells enhances their affinity for the myosin II motor domain (2011)
  • Author(s): Uyeda T, Iwadate Y, Umeki N, Nagasaki A, Yumura S
  • Journal Title: PLos One Volume: 6 Pages: 26200-26200
  • NAID: 120005350262
  • Peer Reviewed
• [Journal Article] Stretching actin filaments within cells enhances their affinity for the myosin II motor domain (2011)
  • Author(s): Uyeda, et al
  • Journal Title: Plos One Volume: 6
  • Peer Reviewed
• [Journal Article] Cyclic stretch of the substratum using a shape-memory alloy induces directional migration in Dictyostelium cells (2009)
  • Author(s): Iwadate Y. and S. Yumura
  • Journal Title: Biotechniques Volume: 47 Pages: 757-767
  • Peer Reviewed
• [Journal Article] PTEN is a mechanosensing signal transducer for myosin II localization in Dictyostelium c (2009)
  • Author(s): Pramanik Md. K., M. Iijima, Y. Iwadate, and S. Yumura
  • Journal Title: _Genes to Cells Volume: 14 Pages: 821-834
  • Peer Reviewed
• [Journal Article] Cyclic stretch of the substratum using a shape-memory alloy induces directional migration in Dictyostelium cells. (2009)
  • Author(s): Iwadate Y., et al.
  • Journal Title: Biotechniques 47 Pages: 757-767
  • Peer Reviewed
• [Journal Article] PTEN is a mechanosensing signal transducer for myosin II localization in Dictyostelium cells. (2009)
  • Author(s): Pramanik Md.K., et al.
  • Journal Title: Genes to Cells 14 Pages: 821-834
  • Peer Reviewed
• [Presentation] ミオシンIIによる細胞極性制御 (2011)
  • Author(s): 藤本甲子郎, 祐村恵彦
  • Organizer: 山口大学研究推進体
  • Place of Presentation: 山口市
  • Year and Date: 2011-12-20
• [Presentation] 細胞性粘菌の細胞運動の3次元観察 (2011)
  • Author(s): 宇野博之, 祐村恵彦
  • Organizer: 山口大学研究推進体
  • Place of Presentation: 山口市
  • Year and Date: 2011-12-20
• [Presentation] 細胞質分裂に関する新規遺伝子の網羅的検索 (2011)
  • Author(s): 山内大史, 祐村恵彦
  • Organizer: 山口大学研究推進体
  • Place of Presentation: 山口市
  • Year and Date: 2011-12-20
• [Presentation] ミオシンIIによる仮足形成の制御 (2011)
  • Author(s): 藤本甲子郎, 祐村恵彦
  • Organizer: 日本生化学会
  • Place of Presentation: 国立京都国際会館京都市
  • Year and Date: 2011-09-22
• [Presentation] Role of Dynamin in cytokinesis of Dictyostelium (2011)
  • Author(s): A. Y. K. Md. Masud Rana and S. Yumura
  • Organizer: 日本生化学会
  • Place of Presentation: 国立京都国際会館京都市
  • Year and Date: 2011-09-22
• [Presentation] 細胞極性におけるミオシンIIの役割 (2011)
  • Author(s): 藤本甲子郎
  • Organizer: 細胞運動研究会
  • Place of Presentation: 広島大学,東広島市
  • Year and Date: 2011-09-10
• [Presentation] Dynamins contributes to the cytokinesis and cell migration in Dictyostelium (2011)
  • Author(s): A. Y. K. Md. Masud Rana S Miyagishima, and S. Yumura.
  • Organizer: 細胞運動研究会
  • Place of Presentation: 広島大学,東広島市
  • Year and Date: 2011-09-10
• [Presentation] Regulation of cell polarity by myosin II (2011)
  • Author(s): K. Fujimoto and S. Yumura
  • Organizer: 日本細胞生物学会大会
  • Place of Presentation: 北海道大学(札幌)
  • Year and Date: 2011-06-27
• [Presentation] Dynamin affects cytokinesis and cell migration in Dictyostelium cells (2011)
  • Author(s): A. Y. K. Md. Masud Rana and S. Yumura
  • Organizer: 日本細胞生物学会大会
  • Place of Presentation: 北海道大学(札幌)
  • Year and Date: 2011-06-27
• [Presentation] Stretched Actin Filaments in Cells Have a High Affinity for S1 of Myosin II (2010)
  • Author(s): Uyeda, et al
  • Organizer: 米国生物物理学会シンポジウムActin, the Cytoskeleton and the Nucleus
  • Place of Presentation: シンガポール共和国・シンガポール
  • Year and Date: 2010-11-09
• [Presentation] Mechanism for association of actin and myosin II filaments at the equatorial region during cytokinesis in Dictyopstelium. (2010)
  • Author(s): Uematsu, et al.
  • Organizer: 日本細胞生物学会ワークショップ
  • Place of Presentation: 大阪・大阪国際会議場
  • Year and Date: 2010-05-20
• [Presentation] Talin A couples acto-myosin bundles to the plasma membrane. (2010)
  • Author(s): Tsujioka, et al.
  • Organizer: 日本細胞生物学会ワークショップ
  • Place of Presentation: 大阪・大阪国際会議場
  • Year and Date: 2010-05-20
• [Presentation] Dynamins are involved in cytokinesis of Dictyostelium (2010)
  • Author(s): Rena, et al.
  • Organizer: 日本細胞生物学会
  • Place of Presentation: 大阪・大阪国際会議場
  • Year and Date: 2010-05-20
• [Presentation] Mechanism for association of actin and myosin II filaments at the equatorial region during cytokinesis in Dictyostelium discoideum (2010)
  • Author(s): N. Uematsu and S. Yumura
  • Organizer: 日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場大阪市
  • Year and Date: 2010-05-19
• [Presentation] S. Yonemura Talin A couples acto-myosin bundles to the plasma membrane (2010)
  • Author(s): M. Tsujioka, S. Yumura
  • Organizer: 日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場大阪市
  • Year and Date: 2010-05-19
• [Presentation] Dynamins are involved in cytokinesis of Dictyostelium (2010)
  • Author(s): R. Masud, S. Miyagishima, S. Yumura
  • Organizer: 日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場大阪市
  • Year and Date: 2010-05-19
• [Presentation] Stretched actin filaments in cells have a high affinity for S1 of myosin II (2010)
  • Author(s): T. Uyeda, Y. Iwadate, S. Yumura, and A. Nagasaki
  • Organizer: 米国生物物理学会シンポジウム
  • Place of Presentation: シンガポール
  • Year and Date: 2010-01-09
• [Presentation] A possible mechanism of directional migration in Dictyostelium cells induced by the cyclic stretch and shrinkage of the substratum (2009)
  • Author(s): Y. Iwadate and S. Yumura
  • Organizer: 日本生物物理学会
  • Place of Presentation: 徳島文理大学徳島市
  • Year and Date: 2009-10-30
• [Presentation] 細胞性粘菌の分裂時のアクチン、ミオシンの分裂面への集合機構 (2009)
  • Author(s): Uemastu, et al.
  • Organizer: 第12回細胞性粘菌研究会
  • Place of Presentation: 山口大学大学会館
  • Year and Date: 2009-10-20
• [Presentation] Cell migration of Dictyostelium cells in three-dimensional environment. (2009)
  • Author(s): Kuwabara, et al.
  • Organizer: 第61回日本細胞生物学会大学
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-06-03
• [Presentation] PTEN contributes to myosi II locahzation by mnechanosensing signal in Dictvostelium cells. (2009)
  • Author(s): Pramanik, et al.
  • Organizer: 第61回日本細胞生物学会大学
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-06-03
• [Book] 部分執筆(前田靖男編)細胞運動分裂の分子モーター「モデル生物:細胞性粘菌」 (2012)
  • Author(s): 祐村恵彦・上田太郎
  • Publisher: アイ.ピー.シー社
• [Book] 細胞運動,分裂の分子モーター「モデル生物:細胞性粘菌」 (2012)
  • Author(s): 祐村恵彦・上田太郎, (前田靖男編)
  • Publisher: アイ.ピー.シー社(未定)
• [Book] 研究をささえるモデル生物(吉川寛他編部分執筆) (2009)
  • Author(s): 祐村恵彦
• [Book] ゲノム時代のモデル生物 (2009)
  • Author(s): 吉川寛, ら編
  • Total Pages: 3
  • Publisher: 化学同人
• [Remarks]
  • URL: http://web.cc.yamaguchi-u.ac.jp/?nenkin/TOPYUMURA.html