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The molecular mechanism and the physiological role of vacuolar amino acid pool formation

Research Project

Project/Area Number 21570200
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionEhime University

Principal Investigator

SEKITO Takayuki  愛媛大学, 農学部, 助教 (20419857)

Co-Investigator(Renkei-kenkyūsha) FUJIKI Yuki  埼玉大学, 大学院・理工学研究科, 助教 (00414011)
KAKINUMA Yoshimi  愛媛大学, 農学部, 教授 (80134394)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords細胞機能 / 構造 / 液胞 / アミノ酸トランスポーター / VBAファミリー / AVTファミリー / Major Facilitator Superfamily
Research Abstract

This study suggests that Avt3 and Avt4 in Saccharomyces cerevisiae AVT transporter family are redundantly involved in the export of a broad range of neutral amino acids from vacuoles, and that Avt4 but not Avt3 functions to extrude vacuolar basic amino acids during nitrogen starvation. On the other hand, Avt1 was suggested to uptake various neutral amino acids and histidine into vacuoles. In addition, we newly identified 13 transporters localized to the vacuolar membrane, and found that some of these transporters may be involved in the vacuolar amino acid transport.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (45 results)

All 2012 2011 2010 2009 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (32 results) Remarks (1 results)

  • [Journal Article] Atg22p, a vacuolar membrane protein involved in the amino acid compartmentalization of Schizosaccharomyces pombe2011

    • Author(s)
      N. Sugimoto, T. Iwaki, S. Chardwiriyapreecha, M. Shimazu, M. Kawano, T. Sekito, K. Takegawa and Y. Kakinuma
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 75 Pages: 385-387

    • NAID

      10027898232

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Atg22p, a vacuolar membrane protein involved in the amino acid compartmentalization of Schizosaccharomyces pombe2011

    • Author(s)
      Naoko Sugimoto
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: Vol.75 Pages: 385-387

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vba2p, a vacuolar membrane protein involved in basic amino acid transport in Schizosaccharomyces pombe2010

    • Author(s)
      N. Sugimoto, T. Iwaki, S. Chardwiriyapreecha, M. Shimazu, T. Sekito, K. Takegawa and Y. Kakinuma
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 74

    • NAID

      10027561068

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Avt5p is required for vacuolar uptake of amino acids in the fission yeast Schizosaccharomyces pombe2010

    • Author(s)
      S. Chardwiriyapreecha, H. Mukaiyama, T. Sekito, T. Iwaki, K. Takegawa and Y. Kakinuma
    • Journal Title

      FEBS Lett.

      Volume: 584 Pages: 2339-2345

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Vba2p, a vacuolar membrane protein involved in basic amino acid transport in Schizosaccharomyces pombe2010

    • Author(s)
      Naoko Sugimoto
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: Vol.74 Pages: 2166-2169

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vacuolar amino acid transporter Avt5p is responsible for lithium uptake in Schizosaccharomyces pombe2010

    • Author(s)
      Tomoko Iwaki
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: Vol.74 Pages: 1719-1721

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Avt5p is required for vacuolar uptake of amino acids in the fission yeast Schizosaccharomyces pombe2010

    • Author(s)
      Soracom Chardwiriyapreecha
    • Journal Title

      FEBS Letters

      Volume: Vol.584 Pages: 2339-2345

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Atg17 recruits Atg9 to organize the pre-autophagosomal structure2009

    • Author(s)
      T. Sekito, T. Kawamata, R. Ichikawa, K. Suzuki and Y. Ohsumi
    • Journal Title

      Genes Cells.

      Volume: 14 Pages: 525-538

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evidence for Avt6 as a vacuolar exporter of acidic amino acids in Saccharomyces cerevisiae cells2009

    • Author(s)
      T. Chahomchuen, K. Hondo, M. Ohsaki, T. Sekito and Y. Kakinuma
    • Journal Title

      J. Gen. Appl. Microbiol.

      Volume: 55 Pages: 409-417

    • NAID

      130004449253

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Atg17 recruits Atg9 to organize the pre-autophagosomal structure2009

    • Author(s)
      Takayuki Sekito
    • Journal Title

      Genes to Cells Vol.14

      Pages: 525-538

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evidence for Avt6 as a vacuolar exporter of acidic amino acids in Saccharomyces cerevisiae cells2009

    • Author(s)
      Thippayarat Chahomchuen
    • Journal Title

      The Journal of General and Applied Microbiology Vol.55

      Pages: 409-417

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A simple and specific procedure to permeabilize the plasma membrane of Schizosaccharomyces pombe2009

    • Author(s)
      Soracom Chardwiriyapreecha
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry Vol.73

      Pages: 2090-2095

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 出芽酵母液胞アミノ酸トランスポーターAvt1は広い基質特異性を有する2012

    • Author(s)
      刀根潤一
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-25
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 出芽酵母における分裂酵母液胞アミノ酸トランスポーターFnx1の機能発現2012

    • Author(s)
      眞鍋邦男
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] 出芽酵母新規液胞アミノ酸トランスポーターの同定2012

    • Author(s)
      森實大貴
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] Functional expression of the Arabidopsis thaliana AVT3A gene for a tonoplast amino acid exporter in Saccharomyces cerevisiae2011

    • Author(s)
      手嶋博光
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-15
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Functional analysis of the N-terminal hydrophilic region of a vacuolar amino acid transporter Avt4 in the budding yeast Saccharomyces cerevisiae2011

    • Author(s)
      石本晶也
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] Avt3p, a vacuolar amino acid transporter in Schizosaccharomyces pombe2011

    • Author(s)
      Soracom Chardwiriyapreecha
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] オートファジーアミノ酸リサイクルにおける液胞アミノ酸トランスポーターAvt3/Avt4の機能2011

    • Author(s)
      関藤孝之
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都国際会館
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Involvement of Vba2p and Atg22p in vacuolar amino acid compartment alization on Schizosaccharomyces pombe2011

    • Author(s)
      Soracom Chardwiriyapreecha
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都国際会館
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] 出芽酵母モノカルボン酸トランスポーターホモログの解析2011

    • Author(s)
      森實大貴
    • Organizer
      第52回日本生化学会中国・四国支部例会
    • Place of Presentation
      広島大学広仁会館
    • Year and Date
      2011-05-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] 出芽酵母液胞アミノ酸トランスポーターAvt1の解析2011

    • Author(s)
      刀根潤一
    • Organizer
      第52回日本生化学会中国・四国支部例会
    • Place of Presentation
      広島大学広仁会館
    • Year and Date
      2011-05-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] 分裂酵母液胞アミノ酸トランスポーターの機能解析2011

    • Author(s)
      眞鍋邦男
    • Organizer
      第52回日本生化学会中国・四国支部例会
    • Place of Presentation
      広島大学広仁会館
    • Year and Date
      2011-05-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] 出芽酵母におけるオートファジーアミノ酸リサイクル機構2011

    • Author(s)
      関藤孝之
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2011-03-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Novel vacuolar membrane proteins, Atg22p and Vba2p, involved in aminoacid transport by Schizosaccharomyces pombe2011

    • Author(s)
      Soracom Chardwiriyapreecha
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2011-03-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] The molecular mechanism of amino acid export from vacuoles in the budding yeast Saccharomyces cerevisiae2010

    • Author(s)
      Soracom Chardwiriyapreecha
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] 出芽酵母の液胞アミノ酸排出系Avt3/Avt4の性質2010

    • Author(s)
      関藤孝之
    • Organizer
      第36回日本生体エネルギー研究会
    • Place of Presentation
      大阪大学
    • Year and Date
      2010-11-19
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Vacuolar amino acid transporters in the fission yeast Schizosaccharomyces pombe2010

    • Author(s)
      Soracom Chardwiriyapreecha
    • Organizer
      28th International Specialized Symposium on Yeasts
    • Place of Presentation
      Montien Riverside Hotel, Bangkok, Thailand
    • Year and Date
      2010-09-17
    • Related Report
      2011 Final Research Report
  • [Presentation] Vacuolar amino acid transporters in the fission yeast Schizosaccharomyces pombe2010

    • Author(s)
      Soracom Chardwiriyapreecha
    • Organizer
      28th International Specialized Symposium on Yeasts : Metabolic and Bioprocess Engineering for Sustainable Development
    • Place of Presentation
      Montien Riverside Hotel, Bangkok, Thailand
    • Year and Date
      2010-09-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] 出芽酵母液胞からのアミノ酸排出経路の解析2010

    • Author(s)
      阪村慧
    • Organizer
      第51回日本生化学会 中国・四国支部例会
    • Place of Presentation
      山口大学
    • Year and Date
      2010-05-14
    • Related Report
      2010 Annual Research Report
  • [Presentation] Involvement of Avt3p in vacuolar amino acid transport of fission yeast Schizosaccharomyces pombe2010

    • Author(s)
      Soracom Chardwiriyapreecha
    • Organizer
      第51回日本生化学会 中国・四国支部例会
    • Place of Presentation
      山口大学
    • Year and Date
      2010-05-14
    • Related Report
      2010 Annual Research Report
  • [Presentation] Amino acid recycling by vacuolar amino acid transporters Avt3 and Avt4 during autophagy of the budding yeast Saccharomyces cerevisiae2010

    • Author(s)
      Takayuki Sekito
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 液胞内アミノ酸リサイクルにおける液胞アミノ酸トランスポーターの役割2009

    • Author(s)
      関藤孝之
    • Organizer
      日本生体エネルギー研究会
    • Place of Presentation
      旭川医科大学
    • Year and Date
      2009-12-20
    • Related Report
      2011 Final Research Report
  • [Presentation] 液胞内アミノ酸リサイクルにおける液胞アミノ酸トランスポーターの役割2009

    • Author(s)
      関藤孝之
    • Organizer
      日本生体エネルギー研究会第35回討論会
    • Place of Presentation
      旭川医科大学
    • Year and Date
      2009-12-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] 液胞アミノ酸トランスポーターをコードする分裂酵母SPBC1685.07遺伝子の解析2009

    • Author(s)
      柿沼喜己
    • Organizer
      日本生体エネルギー研究会第35回討論会
    • Place of Presentation
      旭川医科大学
    • Year and Date
      2009-12-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] The role of AVT vacuolar amino acid transporter family in amino acid recycling during autophagy in the budding yeast Saccharomyces cerevisiae2009

    • Author(s)
      関藤孝之
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-11
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] Physiological role and regulation of VBA vacuolar amino acid transporter family in the budding yeast Saccharomyces cerevisiae2009

    • Author(s)
      柿沼喜己
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] Role of Avt8p in vacuolar amino acid compartmentalization of Schizosaccharomyces pombe2009

    • Author(s)
      Soracom Chardwiriyapreecha
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] Functional characterization of Avt/LYAAT1-related vacuolar amino acid transporters in Arabidopsis2009

    • Author(s)
      藤木友紀
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] 液胞アミノ酸トランスポーターAVTファミリーの機能解析2009

    • Author(s)
      加藤秀一
    • Organizer
      第27回 Yeast Workshop
    • Place of Presentation
      飯塚研究開発センター
    • Year and Date
      2009-11-14
    • Related Report
      2009 Annual Research Report
  • [Presentation] 液胞アミノ酸トランスポーターVba1/Vba4の窒素飢餓応答2009

    • Author(s)
      吉村鮎美
    • Organizer
      第27回 Yeast Workshop
    • Place of Presentation
      飯塚研究開発センター
    • Year and Date
      2009-11-14
    • Related Report
      2009 Annual Research Report
  • [Presentation] 出芽酵母液胞VBAアミノ酸トランスポーターの発現変動とその生理機能解析2009

    • Author(s)
      柿沼喜己
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2009-10-22
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] 酵母オートファジーにおけるAVT液胞アミノ酸トランスポーターファミリーの役割2009

    • Author(s)
      関藤孝之
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2009-10-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] A vacuolar transporter Avt8p for acidic amino acids in Schizosaccharomyces pombe2009

    • Author(s)
      Soracom Chardwiriyapreecha
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2009-10-22
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://web-abs.agr.ehime-u.ac.jp/mpg/mpg-j/index.htm

    • Related Report
      2011 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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