| Project/Area Number |
21570204
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Takasaki University of Health and Welfare |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
UMATA Toshiyuki 産業医科大学, 産業医学研究支援施設, 准教授 (30213482)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | Mina53 / アレルギー / リボソーム / 喘息 / IL4 / 気道過敏性 / 炎症性細胞浸潤 / 遺伝子 / 核酸 / 細胞・組織 / KDM2A / リボソームRNA / コハク酸 / ピストン脱メチル化酵素 / 転写 / JmjC / MINA |
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| Research Abstract |
Asthma is a highly prevalent chronic respiratory disease affecting 300 million people world-wide. Allergic asthma is the most common form of asthma, a Th2-biased disease, and triggered by inhaling allergens such as house dust mites(HDM). In addition to the environmental factors, genetic factors are also involved in asthma. However, the interaction of these factors is complex and not fully understood. It was recently reported that Mina53 was a necessary and sufficient dose-dependent IL-4-specific 'repressor' in naive helper T cells, using an ex vivo system of Th2-differentiation from naive helper T cells(Nat Immunol. 2009 ; 10 : 872-9). However, there have been no experiments investigating the role of Mina53 in the Th2-bias in vivo in animals. While it had been reported that Mina53(myc-induced nuclear antigen with a 53 kDa molecular mass ; also known as mina) is associated with tumorigenesis, it was not clear what role Mina53 plays in non-neoplastic tissues. To directly address the functions of Mina53 in a normal body, we created mina53-deficient mice. While both male and female mina53-deficient mice grew to adulthood and were fertile, we found that Mina53 functions as an 'activator' in the allergic response, in asthma, possibly through controlling IL-4 production. We also analyzed the effect of succinate on ribosome biogenesis, and found that succinate inhibited another JmjC protein KDM2A to increase the rDNA transcription.
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