• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis for the two distinct pathways to form mitotic spindles in C. elegans embryos

Research Project

Project/Area Number 21570209
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

TOYA Mika  独立行政法人理化学研究所, 高次構造形成研究グループ, 研究員 (80455360)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords紡錘体形成 / 微小管制御 / オーロラキナーゼA / γチューブリン / 線虫初期胚 / ライブイメージング / RNAi / γ-チューブリン
Research Abstract

The evolutionarily conservedγ-tubulin is the main microtubule nucleator. C. elegans embryos have another,γ-tubulin-independent, microtubule assembly mechanism that requires Aurora A kinase(AIR-1). This study showed that AIR-1 stabilizes the spindle microtubules in a kinase-independent manner in addition to its kinase-dependent role at centrosomes. The kinase-independent AIR-1 was crucial in the assembly of chromatin-stimulated microtubules whileγ-tubulin complex was dispensable for the process. The results obtained suggested that the roles of aγ-tubulin complex, a kinase-dependent AIR-1, and a kinase-independent AIR-1 necessary to be coordinated to assemble functional mitotic spindles.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (21 results)

All 2011 2010 2009 Other

All Journal Article (5 results) (of which Peer Reviewed: 4 results) Presentation (12 results) Book (3 results) Remarks (1 results)

  • [Journal Article] A kinase-independent role for Aurora A in the assembly of mitotic spindle microtubules in Caenorhabditis elegans embryos2011

    • Author(s)
      Toya, M., Terasawa, M., Nagata, K., Iida, Y. and Sugimoto, A.
    • Journal Title

      Nat. Cell Biol

      Volume: 13 Pages: 708-715

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A kinase-independent role for Aurora A in the assembly of mitotic spindle microtubules in Caenorhabditis elegans embryos2011

    • Author(s)
      Toya M., et al
    • Journal Title

      Nature Cell Biology

      Volume: 13 Issue: 6 Pages: 708-715

    • DOI

      10.1038/ncb2242

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Caenorhabditis elegans ortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bridging DNC-1/p150(Glued) and DNC-2/dynamitin2010

    • Author(s)
      Terasawa, M., Toya, M., Motegi, F., Mana, M., Nakamura, K. and Sugimoto, A.
    • Journal Title

      Genes Cells

      Volume: 15 Pages: 1145-1157

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Caenorhabditis elegans ortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bringing DNC-1/p150(Glued) and DNC-2/dynamitin2010

    • Author(s)
      Terasawa M., et al.
    • Journal Title

      Genes to Cells

      Volume: 15 Pages: 1145-1157

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2009

    • Author(s)
      Masamitsu Sato
    • Journal Title

      Methods in Molecular Biology 545 Mitosis(Humana Press(Springer))

      Pages: 185-204

    • Related Report
      2009 Annual Research Report
  • [Presentation] Contribution of the kinase-inactive-form of Aurora A kinase to the assembly of mitotic spindles in C. elegans embryos2010

    • Author(s)
      戸谷美夏、杉本亜砂子
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
    • Related Report
      2011 Final Research Report
  • [Presentation] Contribution of the kinase-inactive-form of Aurora A kinase to the assembly of mitotic spindles in C.elegans embryos2010

    • Author(s)
      戸谷美夏
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Coordination of two microtubule assembly mechanisms in the formation of mitotic spindles in C. elegans embryos2010

    • Author(s)
      Toya, M., Sugimoto, A.
    • Organizer
      4^<th> East Asia C. elegans Meeting
    • Place of Presentation
      Tokyo
    • Year and Date
      2010-07-13
    • Related Report
      2011 Final Research Report
  • [Presentation] Coordination of two microtubule assembly mechanisms in the formation of mitotic spindles in C.elegans embryos2010

    • Author(s)
      Toya M.
    • Organizer
      4^<th> East Asia C.elegans Meeting
    • Place of Presentation
      東京
    • Year and Date
      2010-07-13
    • Related Report
      2010 Annual Research Report
  • [Presentation] Coordination of two microtubule assembly mechanisms in the formation of mitotic spindles in C. elegans embryos2010

    • Author(s)
      Toya, M., Sugimoto, A.
    • Organizer
      EMBO conference Series Microtubules
    • Place of Presentation
      Heidelberg, Germany
    • Year and Date
      2010-06-04
    • Related Report
      2011 Final Research Report
  • [Presentation] Coordination of two microtubule assembly mechanisms in the formation of mitotic spindles in C.elegans embryos2010

    • Author(s)
      Toya M.
    • Organizer
      EMBO Conference Series Microtubules
    • Place of Presentation
      Heidelberg, Germany
    • Year and Date
      2010-06-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] Coordination of two microtubule assembly mechanisms in the formation of mitotic spindles in C. elegans embyos2010

    • Author(s)
      戸谷美夏、杉本亜砂子
    • Organizer
      第62回日本細胞生物学会大会
    • Place of Presentation
      大阪
    • Year and Date
      2010-05-19
    • Related Report
      2011 Final Research Report
  • [Presentation] Coordination of two microtubule assembly mechanisms in the formation of mitotic spindles in C.elegans embryos2010

    • Author(s)
      戸谷美夏
    • Organizer
      第62回 日本細胞生物学会大会
    • Place of Presentation
      大阪
    • Year and Date
      2010-05-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] Coordination of two distinct pathways to form mitotic spindle microtubules in C. elegans embryos2009

    • Author(s)
      戸谷美夏、杉本亜砂子
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-10
    • Related Report
      2011 Final Research Report
  • [Presentation] Coordination of two distinct pathways to form mitotic spindle microtubules in C.elegans embryos2009

    • Author(s)
      戸谷美夏
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜市、パシフィコ横浜
    • Year and Date
      2009-12-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] 紡錘体形成に関わる2つの微小管経路2009

    • Author(s)
      戸谷美夏、杉本亜砂子
    • Organizer
      第61回日本細胞生物学会大会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-06-04
    • Related Report
      2011 Final Research Report
  • [Presentation] 紡錘体形成に関わる2つの微小管制御経路2009

    • Author(s)
      戸谷美夏
    • Organizer
      第61回日本細胞生物学会大会
    • Place of Presentation
      名古屋市、名古屋国際会議場
    • Year and Date
      2009-06-04
    • Related Report
      2009 Annual Research Report
  • [Book] Microtubules in vivo : Chapter 19, Imaging of mitotic spindle dynamics in Caenorhabditis elegans embryos2010

    • Author(s)
      Toya, M., Iida, Y. and Sugimoto, A.
    • Publisher
      Academic Press Methods in Cell Biology
    • Related Report
      2011 Final Research Report
  • [Book] Methods in Cell Biology, Microtubules in vivo (Chapter 19を担当)2010

    • Author(s)
      Toya M., Iida Y., Sugimoto A.
    • Total Pages
      12
    • Publisher
      Academic Press
    • Related Report
      2010 Annual Research Report
  • [Book] Methods in Molecular Biology 545 Mitosis : Visualization of fluoresence-Tagged proteins in fission yeast : The analysis micotic spindle dynamics using GFP-tubulin under the native promoter2009

    • Author(s)
      Sato, M., Toya, M. and Toda. T.
    • Publisher
      Humana Press(Springer)
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.cdb.riken.jp/jp/04_news/articles/11/110710_auroraa.html

    • Related Report
      2011 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi