| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
The evolutionarily conservedγ-tubulin is the main microtubule nucleator. C. elegans embryos have another,γ-tubulin-independent, microtubule assembly mechanism that requires Aurora A kinase(AIR-1). This study showed that AIR-1 stabilizes the spindle microtubules in a kinase-independent manner in addition to its kinase-dependent role at centrosomes. The kinase-independent AIR-1 was crucial in the assembly of chromatin-stimulated microtubules whileγ-tubulin complex was dispensable for the process. The results obtained suggested that the roles of aγ-tubulin complex, a kinase-dependent AIR-1, and a kinase-independent AIR-1 necessary to be coordinated to assemble functional mitotic spindles.
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