Analysis for the two distinct pathways to form mitotic spindles in C. elegans embryos

• Project/Area Number: 21570209
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: TOYA Mika 独立行政法人理化学研究所, 高次構造形成研究グループ, 研究員 (80455360)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 紡錘体形成 / 微小管制御 / オーロラキナーゼA / γチューブリン / 線虫初期胚 / ライブイメージング / RNAi / γ-チューブリン

Research Abstract

The evolutionarily conservedγ-tubulin is the main microtubule nucleator. C. elegans embryos have another,γ-tubulin-independent, microtubule assembly mechanism that requires Aurora A kinase(AIR-1). This study showed that AIR-1 stabilizes the spindle microtubules in a kinase-independent manner in addition to its kinase-dependent role at centrosomes. The kinase-independent AIR-1 was crucial in the assembly of chromatin-stimulated microtubules whileγ-tubulin complex was dispensable for the process. The results obtained suggested that the roles of aγ-tubulin complex, a kinase-dependent AIR-1, and a kinase-independent AIR-1 necessary to be coordinated to assemble functional mitotic spindles.

Research Products

• [Journal Article] A kinase-independent role for Aurora A in the assembly of mitotic spindle microtubules in Caenorhabditis elegans embryos (2011)
  • Author(s): Toya, M., Terasawa, M., Nagata, K., Iida, Y. and Sugimoto, A.
  • Journal Title: Nat. Cell Biol Volume: 13 Pages: 708-715
  • Peer Reviewed
• [Journal Article] A kinase-independent role for Aurora A in the assembly of mitotic spindle microtubules in Caenorhabditis elegans embryos (2011)
  • Author(s): Toya M., et al
  • Journal Title: Nature Cell Biology Volume: 13 Issue: 6 Pages: 708-715
  • DOI: 10.1038/ncb2242
  • Peer Reviewed
• [Journal Article] Caenorhabditis elegans ortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bridging DNC-1/p150(Glued) and DNC-2/dynamitin (2010)
  • Author(s): Terasawa, M., Toya, M., Motegi, F., Mana, M., Nakamura, K. and Sugimoto, A.
  • Journal Title: Genes Cells Volume: 15 Pages: 1145-1157
  • Peer Reviewed
• [Journal Article] Caenorhabditis elegans ortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bringing DNC-1/p150(Glued) and DNC-2/dynamitin (2010)
  • Author(s): Terasawa M., et al.
  • Journal Title: Genes to Cells Volume: 15 Pages: 1145-1157
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Masamitsu Sato
  • Journal Title: Methods in Molecular Biology 545 Mitosis(Humana Press(Springer)) Pages: 185-204
• [Presentation] Contribution of the kinase-inactive-form of Aurora A kinase to the assembly of mitotic spindles in C. elegans embryos (2010)
  • Author(s): 戸谷美夏、杉本亜砂子
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] Contribution of the kinase-inactive-form of Aurora A kinase to the assembly of mitotic spindles in C.elegans embryos (2010)
  • Author(s): 戸谷美夏
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] Coordination of two microtubule assembly mechanisms in the formation of mitotic spindles in C. elegans embryos (2010)
  • Author(s): Toya, M., Sugimoto, A.
  • Organizer: 4^<th> East Asia C. elegans Meeting
  • Place of Presentation: Tokyo
  • Year and Date: 2010-07-13
• [Presentation] Coordination of two microtubule assembly mechanisms in the formation of mitotic spindles in C.elegans embryos (2010)
  • Author(s): Toya M.
  • Organizer: 4^<th> East Asia C.elegans Meeting
  • Place of Presentation: 東京
  • Year and Date: 2010-07-13
• [Presentation] Coordination of two microtubule assembly mechanisms in the formation of mitotic spindles in C. elegans embryos (2010)
  • Author(s): Toya, M., Sugimoto, A.
  • Organizer: EMBO conference Series Microtubules
  • Place of Presentation: Heidelberg, Germany
  • Year and Date: 2010-06-04
• [Presentation] Coordination of two microtubule assembly mechanisms in the formation of mitotic spindles in C.elegans embryos (2010)
  • Author(s): Toya M.
  • Organizer: EMBO Conference Series Microtubules
  • Place of Presentation: Heidelberg, Germany
  • Year and Date: 2010-06-04
• [Presentation] Coordination of two microtubule assembly mechanisms in the formation of mitotic spindles in C. elegans embyos (2010)
  • Author(s): 戸谷美夏、杉本亜砂子
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪
  • Year and Date: 2010-05-19
• [Presentation] Coordination of two microtubule assembly mechanisms in the formation of mitotic spindles in C.elegans embryos (2010)
  • Author(s): 戸谷美夏
  • Organizer: 第62回 日本細胞生物学会大会
  • Place of Presentation: 大阪
  • Year and Date: 2010-05-19
• [Presentation] Coordination of two distinct pathways to form mitotic spindle microtubules in C. elegans embryos (2009)
  • Author(s): 戸谷美夏、杉本亜砂子
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] Coordination of two distinct pathways to form mitotic spindle microtubules in C.elegans embryos (2009)
  • Author(s): 戸谷美夏
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市、パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] 紡錘体形成に関わる2つの微小管経路 (2009)
  • Author(s): 戸谷美夏、杉本亜砂子
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-04
• [Presentation] 紡錘体形成に関わる2つの微小管制御経路 (2009)
  • Author(s): 戸谷美夏
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋市、名古屋国際会議場
  • Year and Date: 2009-06-04
• [Book] Microtubules in vivo : Chapter 19, Imaging of mitotic spindle dynamics in Caenorhabditis elegans embryos (2010)
  • Author(s): Toya, M., Iida, Y. and Sugimoto, A.
  • Publisher: Academic Press Methods in Cell Biology
• [Book] Methods in Cell Biology, Microtubules in vivo (Chapter 19を担当) (2010)
  • Author(s): Toya M., Iida Y., Sugimoto A.
  • Total Pages: 12
  • Publisher: Academic Press
• [Book] Methods in Molecular Biology 545 Mitosis : Visualization of fluoresence-Tagged proteins in fission yeast : The analysis micotic spindle dynamics using GFP-tubulin under the native promoter (2009)
  • Author(s): Sato, M., Toya, M. and Toda. T.
  • Publisher: Humana Press(Springer)
• [Remarks]
  • URL: http://www.cdb.riken.jp/jp/04_news/articles/11/110710_auroraa.html