Mechanism of postmitotic nuclear membrane assembly by targeting of nuclear membrane proteins to nuclear subdomains
Project/Area Number |
21570211
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Cell biology
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Research Institution | The Institute of Physical and Chemical Research |
Principal Investigator |
FUNAKOSHI Tomoko 独立行政法人理化学研究所, ライブセル分子イメージング研究チーム, 基幹研究所研究員 (90318460)
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Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 核 / 核膜 / 核膜孔 / 膜動態 / 再構成 / イメージング / 核膜孔複合 / 核膜孔複合体 / リアルタイムイメージング |
Research Abstract |
To analyze mechanism of postmitotic NE assembly, we tried to reconstitute and visualize NE subdomain formation in a cell-free system using semi-intact mitotic human cells. Using this assay system, it was shown that INM proteins localized to metaphase chromosomes in a cytosol and ATP/GTP dependent manner and that different cytosol factor would be required for localization of each INM protein to metaphase chromosomes. We further showed that the cytosol dependency is not maintained during mitosis and that dephosphorylation activity is needed for the cytosol-independent step. We reported that a nucleoporin that required for the first step of nuclear pore complex assembly, organizes INM protein accumulation on the chromosome in living cells.
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Report
(4 results)
Research Products
(35 results)
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[Journal Article] Nuclear pore formation but not nuclear growth is governed by cyclin-dependent kinases(Cdks) during interphase2010
Author(s)
Maeshima K, Iino H, Hihara S, Funakoshi T, Watanabe A, Nishimura M, Nakatomi R, Yahata K, Imamoto F, Hashikawa T, Yokota H, Imamoto N
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Journal Title
Nature Mol Struct Biol
Volume: 17
Pages: 1065-1071
Related Report
Peer Reviewed
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[Journal Article] Nuclear pore formation but not nuclear growth is governed by cyclin-dependent kinases (Cdks) during interphase.2010
Author(s)
K.Maeshima, H.Iino, S.Hihara, T.Funakoshi, A.Watanabe, M.Nishimura, R.Nakatomi, K.Yahata, F.Imamoto, T.Hashikawa, H.Yota, N.Imamoto
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Journal Title
Nat.Struct.Mol.Biol.
Volume: 17
Pages: 1065-1071
Related Report
Peer Reviewed
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