| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
To analyze mechanism of postmitotic NE assembly, we tried to reconstitute and visualize NE subdomain formation in a cell-free system using semi-intact mitotic human cells. Using this assay system, it was shown that INM proteins localized to metaphase chromosomes in a cytosol and ATP/GTP dependent manner and that different cytosol factor would be required for localization of each INM protein to metaphase chromosomes. We further showed that the cytosol dependency is not maintained during mitosis and that dephosphorylation activity is needed for the cytosol-independent step. We reported that a nucleoporin that required for the first step of nuclear pore complex assembly, organizes INM protein accumulation on the chromosome in living cells.
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