In vivo imaging of embryonic cell death in the new model animal and its molecular analysis

• Project/Area Number: 21570223
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Kyoto University
• Principal Investigator: SAKAMAKI Kazuhiro 京都大学, 大学院・生命科学研究科, 准教授 (20271017)
• Co-Investigator(Kenkyū-buntansha): UENO Naoto 基礎生物学研究所, 形態形成研究部門, 教授 (40221105)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 発生遺伝 / 細胞死 / 胚発生 / アフリカツメガエル / カスパーゼ / アポトーシス / トランスジェニックカエル / モニター分子 / 可視化 / イメージング

Research Abstract

FADD is an adaptor protein that transmits apoptotic signals in the cell. In this study, we have shown that FADD has multiple functions in Xenopus embryos; it plays roles in heart formation as well as apoptosis. We also established a transgenic frog line expressing a biosensor, which is suitable for detecting dying cells, and observed the change of the biosensor in developing embryos when apoptosis was induced

Research Products

• [Journal Article] Multiple functions of FADD in apoptosis, NF- κB-related signaling, and heart development in Xenopus embryos (2012)
  • Author(s): Kazuhiro Sakamaki、Chiyo Takagi、Atsushi Kitayama 、 Tomoko Kurata 、 Takamasa S. Yamamoto、 Kumiko Chiba、 Katsuya Kominami、Sang-Kee Jung、 Katsuya Okawa、 Masami Nozaki、Hiroshi Y. Kubota 、Naoto Ueno
  • Journal Title: Genes to Cells Volume: 17巻 Issue: 11 Pages: 875-896
  • DOI: 10.1111/gtc.12004
  • Peer Reviewed
• [Presentation] Analysis of programmed cell death in Xenoupus embryos using a FRET-based caspase biosensor (2012)
  • Author(s): Kazuhiro Sakamaki、 Makoto Suzuki、 Chiyo Takagi 、Yusuke Hara 、Katsuya Kominami 、Kumiko Chiba、 Hiroshi Y. Kubota、 Naoto Ueno
  • Organizer: 第45回日本発生生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2012-05-31
• [Presentation] 細胞死誘導アダプター分子 FADD の胚発生における機能解析 (2010)
  • Author(s): 酒巻和弘、 高木知世、 木村春奈、 鄭祥基、大川克也、上野直人
  • Organizer: 第43回日本発生生物学会
  • Place of Presentation: 京都
  • Year and Date: 2010-06-21
• [Presentation] 細胞死誘導アダプター分子FADDの胚発生における機能解析 (2010)
  • Author(s): 酒巻和弘、高木知世、木村春奈、鄭祥基、大川克也、上野直人
  • Organizer: 第43回日本発生生物学会
  • Place of Presentation: 京都
  • Year and Date: 2010-06-21
• [Presentation] Analysis of programmed cell death in Xenoupus embryos using a FRET-based caspase biosensor
  • Author(s): Kazuhiro Sakamaki
  • Organizer: 第45回日本発生生物学会
  • Place of Presentation: 神戸国際会議場