Study for a novel mechanism of developmental regulation via nucleocytoplasmic transport

• Project/Area Number: 21570224
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Osaka University
• Principal Investigator: YASUHARA Noriko 大阪大学, 医学系研究科, 特任助教 (90423152)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 核-細胞質間分子輸送 / 初期胚発生 / importinα / 情報伝達制御 / importin α / 核-細胞質間蛋白質輸送 / 細胞分化 / 胚発生

Research Abstract

We have previously found that the nucleocytoplasmic transport system plays an important role for differentiation of mouse embryonic stem cells. The goal of this research is to investigate the role of transport receptors during embryogenesis. We found that transport receptor importin. family members were differentially expressed during mouse embryogenesis. We also found that one member of importin. family has a specific activity to inhibit the nuclear transport of certain transcription factor. Therefore, it is becoming clear that the nucleocytoplasmic transport machinery is involved in cell differentiation and organism development.

Research Products

• [Journal Article] Cell type-specific transcriptional regulation of the gene encoding importin-α1 (2011)
  • Author(s): Yasunao Kamikawa, Noriko Yasuhara, Yoshihiro Yoneda
  • Journal Title: Experimental Cell Research Volume: 317 Pages: 1970-1978
  • URL: http://dx.doi.org/10.1016/j.yexcr.2011.05.024
  • Peer Reviewed
• [Journal Article] Cross talk between distinct nuclear import pathways enables efficient nuclear import of E47 in conjunction with its partner transcription factors (2011)
  • Author(s): Mehmood, R., et al
  • Journal Title: Mol.Biol.Cell Volume: 22 Issue: 19 Pages: 3715-3724
  • DOI: 10.1091/mbc.e10-10-0809
  • Peer Reviewed
• [Journal Article] Uwe Vinkemeier and Gino Cingolani, Molecular Basis for the Recognition of Phosphorylated STAT1 by Importinα5 (2010)
  • Author(s): Jonathan Nardozzi, Nikola Wenta, Noriko Yasuhara
  • Journal Title: Journal of Molecular Biology Volume: 402 Issue: 1 Pages: 83-100
  • DOI: 10.1016/j.jmb.2010.07.013
  • Peer Reviewed
• [Journal Article] Molecular Basis for the Recognition of Phosphorylated STATI by Importin α5 (2010)
  • Author(s): Jonathan Nardozzi, Nikola Wenta, Noriko Yasuhara, Uwe Vinkemeier, Gino Cingolani
  • Journal Title: Journal of Molecular Biology Volume: 402 Pages: 83-100
  • Peer Reviewed
• [Journal Article] Synergistic nuclear import of NeuroD1 and its partner transcription factor, E47, via heterodimerization (2009)
  • Author(s): Rashid Mehmood
  • Journal Title: Experimental Cell Research 315 Pages: 1639-1652
  • Peer Reviewed
• [Journal Article] The role of the nuclear transport system in cell differentiation (2009)
  • Author(s): Noriko Yasuhara
  • Journal Title: Seminars in Cell & Developmental Biology 20 Pages: 590-599
• [Presentation] The nucleocytoplasmic transport system regulates the stem cell differentiation (2011)
  • Author(s): 安原徳子
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 神奈川みなとみらい横浜
  • Year and Date: 2011-12-15
• [Presentation] The nucleocytoplasmic transport system regulates the stem cell difierentiation (2011)
  • Author(s): 安原徳子
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 神奈川
  • Year and Date: 2011-12-15
• [Presentation] The role of nucleocytoplasmic transport in cell differentiation (2011)
  • Author(s): Noriko Yasuhara
  • Place of Presentation: エルサレム(イスラエル)
  • Year and Date: 2011-11-08
• [Presentation] 核-細胞質間蛋白質輸送システムによる転写制御機構 (2011)
  • Author(s): 安原徳子
  • Organizer: 第10回核ダイナミクス研究会
  • Place of Presentation: 北海道北広島
  • Year and Date: 2011-10-27
• [Presentation] 核-細胞質間蛋白質輸送システムによる転写制御機構 (2011)
  • Author(s): 安原徳子
  • Organizer: 第10回核ダイナミクス研究会
  • Place of Presentation: 北海道
  • Year and Date: 2011-10-27
• [Presentation] 核-細胞質間輸送と細胞分化 (2011)
  • Author(s): 安原徳子
  • Organizer: 第11回日本蛋白質科学会年会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2011-06-09
• [Presentation] 核-細胞質間輸送と細胞分化 (2011)
  • Author(s): 安原徳子
  • Organizer: 第11回日本蛋白質科学会年会
  • Place of Presentation: 大阪(招待講演)
  • Year and Date: 2011-06-09
• [Presentation] Controlling the cell fate by nucleocytoplasmic transport (2011)
  • Author(s): 安原徳子
  • Organizer: International Symposium on the Physicochemical Field for Genetic Activities
  • Place of Presentation: 淡路島夢舞台
  • Year and Date: 2011-01-26
• [Presentation] Controlling the cell fate by nucleocytoplasmic transport (2011)
  • Author(s): 安原徳子
  • Organizer: International Symposium on the Physicochemical Field for Genetic Activities
  • Place of Presentation: 淡路島 夢舞台(招待講演)
  • Year and Date: 2011-01-26
• [Presentation] Nuclear import of E47 and its partner transcription factors involves a cross talk between distinct nuclear import pathways (2010)
  • Author(s): 安原徳子
  • Organizer: 日本分子生物学会、日本生化学会(合同年会)
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-12-08
• [Presentation] Nuclear import of E47 and its partner transcription factors involves a cross talk between distinct nuclear import pathways (2010)
  • Author(s): 安原徳子
  • Organizer: 日本分子生物学会、日本生化学会(合同年会)
  • Place of Presentation: 神戸 国際会議場
  • Year and Date: 2010-12-08
• [Presentation] 核-細胞質間蛋白質輸送システムによる細胞運命決定機構 (2010)
  • Author(s): 安原徳子
  • Organizer: 核ダイナミクス研究会
  • Place of Presentation: 静岡ラフォーレ修善寺
  • Year and Date: 2010-05-29
• [Presentation] 核-細胞質間蛋白質輸送受容体によるES細胞の分化制御機構 (2010)
  • Author(s): 安原徳子
  • Organizer: 染色体ワークショップ
  • Place of Presentation: 静岡
  • Year and Date: 2010-01-22
• [Presentation] 核輸送因子による新たなES細胞の未分化維持機 (2010)
  • Author(s): 安原徳子
  • Organizer: 日本細胞生物学会
  • Place of Presentation: 大阪国際会議場
• [Presentation] The role of the nucleocytoplasmic transport system in cell differentiation (2010)
  • Author(s): 安原徳子
  • Organizer: GCOE国際会議
  • Place of Presentation: 大阪千里ライフサイエンスセンター
• [Presentation] The role of the nucleocytoplasmic transport system in cell differentiation (2010)
  • Author(s): 安原徳子
  • Organizer: GCOE国際会議
  • Place of Presentation: 大阪 千里ライフサイエンスセンター
• [Presentation] 核輸送因子による新たなES細胞の未分化維持機構 (2010)
  • Author(s): 安原徳子
  • Organizer: 日本細胞生物学会
  • Place of Presentation: 大阪 国際会議場
• [Presentation] A novel regulatory system to maintain ES cells through the nuclear-cytoplasmic transport (2009)
  • Author(s): 安原徳子
  • Organizer: 日本生化学会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] The compensatory function of importin α subtypes (2009)
  • Author(s): 安原徳子
  • Organizer: 日本細胞生物学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-02