Analysis of physiological function of uroplakin complex in fertilization of Xenopus eggs

• Project/Area Number: 21570225
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Kobe University
• Principal Investigator: FUKAMI Yasuo 神戸大学, 自然科学系先端融合研究環遺伝子実験センター, 教授 (00156746)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 受精 / 細胞膜マイクロドメイン / Srcチロシンキナーゼ / ウロプラキン複合体 / 三量体GTP結合タンパク質 / PI3キナーゼ / Ca^<2+>イオン上昇 / 受精シグナリング

Research Abstract

Oncogene-related gene product Src is a protein kinase that is indispensable for the successful fertilization of Xenopus eggs. This study has revealed that a protein complex called uroplakin complex, which is originally expressed in urothelial tissues, is also present on the egg membranes and functioning as a negative regulator of the Src.

Research Products

• [Journal Article] Role and Regulation of STAT3Phosphorylation at Ser727in Melanocytes and Melanoma Cells (2012)
  • Author(s): Sakaguchi, M., Oka, M., Iwasaki, T., Fukami, Y., Nishigori, C.
  • Journal Title: J Invest Dermatol Volume: (掲載確定) Issue: 7 Pages: 1877-1885
  • DOI: 10.1038/jid.2012.45
  • Peer Reviewed
• [Journal Article] Structural basis of species differences between human and experimental animal CYP1A1S in metabolism of 3, 3', 4, 4', 5-pentachlorobiphenyl (2011)
  • Author(s): Yamazaki, K., Suzuki, M., Itoh, T., Yamamoto, K., Kanemitsu, M., Matsumura, C., Nakano, T., Sakaki, T., Fukami, Y., Imaishi, H., and Inui, H.
  • Journal Title: J. Biochem Volume: 149(4) Pages: 487-494
  • NAID: 10030584940
  • Peer Reviewed
• [Journal Article] Characterization of Lipovitellin 2 as a Tyrosinephosphorylated Protein in Oocytes, Eggs, and Early Embryos of Xenopus laevis (2011)
  • Author(s): Kushima, S., Mammadova, G., Hasan, A. K. M. M., Fukami, Y., and Sato, K.-I.
  • Journal Title: Zoological Sci Volume: 28 Pages: 550-559
  • Peer Reviewed
• [Journal Article] Physiology and gene expression of the rice landrace Horkuch under salt stress (2011)
  • Author(s): Lisa, L. A., Elias, S. M., Rahman, M. S., Shahid, S., Iwasaki, T., Hasan, A. K. M. M., Kosuge, K., Fukami, Y., and Seraj, Z. I.
  • Journal Title: Func. Plant Biol Volume: 38 Issue: 4 Pages: 282-282
  • DOI: 10.1071/fp10198
  • Peer Reviewed
• [Journal Article] Gamete membrane microdomains and their associated molecules in fertilization signaling (2011)
  • Author(s): Mahbub, Hasan AK., Fukami, Y, Sato K.-I.
  • Journal Title: Mol Reprod Dev Volume: 78(10-11) Pages: 814-30
  • Peer Reviewed
• [Journal Article] Unfertilized frog eggs die by apoptosis following meiotic exit (2011)
  • Author(s): Tokmakov, AA, Iguchi, S, Iwasaki, T, Fukami, Y
  • Journal Title: BMC Cell Biol Volume: 12(1) Pages: 56-56
  • Peer Reviewed
• [Journal Article] Gamete membrane microdomains and their associated molecules in fertilization signaling (2011)
  • Author(s): Mahbub Hasan AK, Fukami Y, Sato K.-I
  • Journal Title: Mol.Reprod.Dev. Volume: 78(10-11) Pages: 814-830
  • Peer Reviewed
• [Journal Article] Unfertilized frog eggs die by apoptosis following meiotic exit (2011)
  • Author(s): Tokmakov AA, Iguchi S, Iwasaki T, Fukami Y
  • Journal Title: BMC Cell Biol Volume: 23;12(1) Pages: 56-69
  • Peer Reviewed
• [Journal Article] Characterization of lipovitellin 2 as a tyrosine-phosphorylated protein in oocytes, eggs and early embryos of Xenopus laevis. (2011)
  • Author(s): Kushima, S., Gunay, M., Hasan, A.K.M.M., Fukami, Y., Sato, K
  • Journal Title: Zool.Sci. Volume: (印刷中)
  • NAID: 40018937058
  • Peer Reviewed
• [Journal Article] Comprehensive bioinformatics analysis of cell-free protein synthesis : identification of multiple protein properties that correlate with successful expression (2010)
  • Author(s): Kurotani, A., Takagi, T., Toyama, M., Shirouzu, M., Yokoyama, S., Fukami, Y., and Tokmakov, A. A.
  • Journal Title: FASEB J Volume: 24(4) Pages: 1095-1104
  • Peer Reviewed
• [Journal Article] Analysis of signal transduction in cell-free extracts and rafts of Xenopus eggs (2010)
  • Author(s): Tokmakov, A. A., Iwasaki, T, Sato K.-I., and Fukami, Y.
  • Journal Title: Methods Volume: 51(1) Pages: 177-182
  • Peer Reviewed
• [Journal Article] Activation of T7RNA polymerase in Xenopus oocytes and cell-free extracts (2010)
  • Author(s): Tokmakov, A. A. and Fukami, Y.
  • Journal Title: Genes Cells Volume: 15(11) Pages: 1136-1144
  • Peer Reviewed
• [Journal Article] Analysis of signal transduction in cell-free extracts and rafts of Xenopus eggs. (2010)
  • Author(s): Tokmakov, A.A., Iwasaki, T, Sato K.-I., Fukami, Y.
  • Journal Title: Methods Volume: 51 Pages: 177-182
  • Peer Reviewed
• [Journal Article] Activation of T7 RNA polymerase in Xenopus oocytes and cell-free extracts. (2010)
  • Author(s): Tokmakov, A., Fukami, Y.
  • Journal Title: Genes Cells Volume: 15 Pages: 1136-1144
  • Peer Reviewed
• [Journal Article] Evidence that phosphatidylinositol 3-kinase is involved in sper-minduced tyrosine kinase signaling in Xenopus egg fertilization. (2010)
  • Author(s): Mammadova, G., Iwasaki, T., Tokmakov, A.A., Fukami, Y., Sato, K.-I.
  • Journal Title: BMC Dev.Biol. (印刷中)
  • Peer Reviewed
• [Journal Article] Analysis of signal transduction in cell-free extracts and rafts of Xenopus eggs. (2010)
  • Author(s): Tokmakov, A.A., Iwasaki, T., Sato, K.-I., Fukami, Y.
  • Journal Title: Methods (印刷中)
  • Peer Reviewed
• [Journal Article] Comparative expression analysis of multiple PDK genes in Xenopus laevis during oogenesis, maturation, fertilization, and early embryogenesis (2009)
  • Author(s): Tokmakov, A. A., Terazawa, Y., Ikeda, M., Shirouzu, M., Fukami, Y., and Yokoyama, S.
  • Journal Title: Gene Exp. Patterns Volume: 9(3) Pages: 158-165
  • Peer Reviewed
• [Journal Article] TPA inhibits melanoma growth by inactivation of STAT3through PKC-activated tyrosine phosphatase(s) (2009)
  • Author(s): Oka, M., Sumita, N., Sakaguchi, M., Iwasaki, T., Bito, T., Kageshita, T., Takeuchi, S., Sato, K., Fukami, Y., and Nishigori, C.
  • Journal Title: J. Biol. Chem Volume: 284(44) Pages: 30416-23
  • Peer Reviewed
• [Journal Article] Evidence that phosphatidylinositol 3-kinase is involved in sperm-induced tyrosine kinase signaling in Xenopus egg fertilization (2009)
  • Author(s): Mammadova, G., Iwasaki, T., Tokmakov, A. A., Fukami, Y., and Sato, K.-I.
  • Journal Title: BMC Dev. Biol Volume: 9 Pages: 68-68
  • Peer Reviewed
• [Journal Article] Comparative expression analysis of multiple PDK genes in Xenopuslaevis during oogenesis, maturation, fertilization, and early embryogenesis. (2009)
  • Author(s): Tokmakov, A.A., Terazawa, Y., Ikeda, M., Shirouzu, M., Fukami, Y., Yokoyama, S.
  • Journal Title: Gene Exp. Patterns. 9(3) Pages: 158-165
  • Peer Reviewed
• [Presentation] Functional deregulation of MAP kinase in apoptotic cells (2011)
  • Author(s): Alexander Tokmakov, Kosuke Akino, Tomoyo Tani, Sho Iguchi, Tetsushi Iwasaki, Yasuo Fukami
  • Organizer: BMB2011第34回分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-16
• [Presentation] Profiling of caspase activity in ageing Xenopus eggs (2011)
  • Author(s): Sho Iguchi, Tetsushi Iwasaki, Yasuo Fukami, Alexander Tokmakov
  • Organizer: BMB2011第34回分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15
• [Presentation] Gene expression regulated by STAT3during Xenopus oocyte maturation (2011)
  • Author(s): Tetsushi Iwasaki, Sho Iguchi, Kosuke Akino, Alexander Tokmakov, Yasuo Fukami
  • Organizer: BMB2011第34回分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15
• [Presentation] アフリカツメガエル配偶子間における膜マイクロドメイン依存性クロストーク (2011)
  • Author(s): マーブブハサンAKM、玖島将太、橋本亜樹、前川由佳、三田勇樹、戸阪智樹、深見泰夫、佐藤賢一
  • Organizer: BMB2011第34回分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] アフリカツメガエル配偶子間における膜マイクロドメイン依存性クロストーク (2011)
  • Author(s): マーブブ ハサン AKM、玖島将太、橋本亜樹、前川由佳、三田勇樹、戸阪智樹、深見泰夫、佐藤賢一
  • Organizer: 第34回分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-13
• [Presentation] Isoform-specific activation of caspases in unfertilized post-meiotic frog eggs (2011)
  • Author(s): Sho Iguchi, Tetsushi Iwasaki, Yasuo Fukami, Alexander Tokmakov
  • Organizer: 日本動物学会第82回旭川大会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-21
• [Presentation] Evolutional and functional analysis of Xenopus STAT3Keystone Symposia on Molecular and Cellular Biology (2011)
  • Author(s): Tetsushi Iwasaki, Sho Iguchi, Tokmakov A. Alexander, Yasuo Fukami
  • Organizer: The Evolution of Protein Phosphorylation
  • Place of Presentation: Keystone, CO, US
  • Year and Date: 2011-01-25
• [Presentation] Functional analysis of STAT3during Xenopus oocyte maturation (2010)
  • Author(s): Tetsushi Iwasaki, Sho Iguchi, Takanori Hashimoto, Minori Sasakura, Tokmakov A. Alexander, Yasuo Fukami
  • Organizer: BMB2010第33回分子生物学会年会、第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Alexander, Biphasic activation of caspase3/7in maturing Xenopus oocytes and post-meiotic eggs (2010)
  • Author(s): Sho Iguchi, Takanori Hashimoto, Tetsushi Iwasaki, Yasuo Fukami, Tokmakov A
  • Organizer: BMB2010第33回分子生物学会年会、第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Biphasic activation of caspase 3/7 in maturing Xenopus oocytes and post-meiotic eggs (2010)
  • Author(s): Iguchi, S., Hashimoto, T., Iwasaki, T., Fukami, Y., Tokmakov, A.
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会合同大会
  • Place of Presentation: 神戸国際展示場(兵庫県)
  • Year and Date: 2010-12-09
• [Presentation] Effect of Src kinase inhibition on melanoma cell growth (2010)
  • Author(s): Tomoyo Tani, Tokmakov A. Alexander, Tetsushi Iwasaki, Yasuo Fukami
  • Organizer: BMB2010第33回分子生物学会年会、第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Serumindependent growth and anti-apoptosis of human bladder carcinoma cells require functions of membrane microdomain-associated proteins such as Src and uroplakin III (2010)
  • Author(s): Ken-ichi Sato, Yukari Kawada, Natsumi Yamamoto, Yasuo Fukami
  • Organizer: BMB2010第33回分子生物学会年会、第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Analysis of Src-dependent gene expression that is regulated under serum starvation conditions in human bladder carcinoma cells(ヒトがん細胞において血清飢餓環境下でSrc依存的に発現制御を受ける遺伝子の解析) (2009)
  • Author(s): 河田有佳里、山本夏海、深見泰夫、佐藤賢一
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] Analysis of gamete interaction and signal transduction in Xenopus laevis by using isolated egg membrane microdomains, iEMD(単離した卵細胞膜マイクロドメイン(iEMD)を用いた配偶子間相互作用と受精シグナリング機構の解析) (2009)
  • Author(s): 佐藤賢一、玖島将太、ママドワギュナイ、マブブハサンAKM、深見泰夫
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] Involvement of phosphatidylinositol 3-kinase in signal transduction at fertilization in Xenopus eggs(ツメガエル卵受精シグナル伝達におけるホスファチジルイノシトール3キナーゼの関与) (2009)
  • Author(s): Mammadova, G., Iwasaki, T., Tokmakov, AA., Fukami, Y., Sato, K
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] Unfertilized Xenopus eggs die by apoptosis after MAPK inactivation (2009)
  • Author(s): Tokmakov, AA., Iguchi, S., Iwasaki, T., Fukami, Y
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Expression analysis of hnRNP K-binding mRNAs during Xenopus early development (2009)
  • Author(s): Iwasaki, T., Hasan, AKM. M., Iguchi, S., Mammadova, G., Tokmakov, AA., Sato, K., Fukami, Y
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Analysis of Xenopus Src and G protein functions in egg activation (2009)
  • Author(s): Hasan, AKM, M., Iwasaki, T., Mammadova, G., Iguchi, S., Tokmakov, AA., Sato, K., Fukami, Y.
  • Organizer: 第82回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] Analysis of Xenopus Src and G protein functions in egg activation. (2009)
  • Author(s): Hasan, A.K.M., Iwasaki, T., Mammadova, G., Iguchi, S., Tokmakov, A.A., Sato, K.-I., Fukami, Y.
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2009-10-22
• [Presentation] TPA inhibits melanoma growth by inactivation of STAT3through PKC-activated tyrosine phosphatase(s) (2009)
  • Author(s): Oka M., Bito T., Sato K., Fukami Y., Nishigori C
  • Organizer: 第86回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-01
• [Presentation] Involvement of Phosphatidylinositol 3-kinase in sperm-induced tyrosine kinase signaling in Xenopus egg fertilization (2009)
  • Author(s): Mammadova, G, , Kushima, S., Iwasaki, T., Tokmakov, AA., Fukami, Y., Sato, K
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡
  • Year and Date: 2009-09-17
• [Presentation] Molecular mechanism of Src activation in Xenopus egg fertilization、Gordon Research Conference (2009)
  • Author(s): Iwasaki T, Hasan AKM M, Tokmakov A., Mammadova G, Sato K, Fukami Y
  • Organizer: Phosphorylation & G-Protein Mediated Signaling Network
  • Place of Presentation: Biddeford, US
• [Remarks]
  • URL: http://www.research.kobe-u.ac.jp/rceg/fukami/index.html
• [Remarks]
  • URL: http://www.research.kobe-u.ac.jp/rceg/fukami/index.html
• [Remarks]
  • URL: http://www.research.kobe-u.ac.jp/rceg/fukami/index.html
• [Remarks]
  • URL: http://www.research.kobe-u.ac.jp/rceg/fukami/index.html