| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Research Abstract |
To quantitate the cell-cell interaction during development, we tried to establish the system for quantification of two reporter genes in sea urchin embryo. However, we could not observe the relation between the levels of two reporter gene expressions. Next we analyzed the expression in mesenchyme cells using knock-in embryos generated by ZFN-mediated gene addition, and found that there was variations in expression levels among primary mesenchyme cells dependent on the changes of cell-cell interaction.
|
