Quantitative analysis of cell-cell interaction responsible for the robustness of development
| Project/Area Number |
21570226
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SAKAKMOTO Naoaki 広島大学, 大学院・理学研究科, 准教授 (00332338)
SHIBATA Tatsuo 理化学研究所, 発生再生科学総合研究センター, ユニットリーダー (10359888)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 発生・分化 / 遺伝子 / ゲノム / 発生制御 / ゆらぎ / 発現制御 |
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| Research Abstract |
To quantitate the cell-cell interaction during development, we tried to establish the system for quantification of two reporter genes in sea urchin embryo. However, we could not observe the relation between the levels of two reporter gene expressions. Next we analyzed the expression in mesenchyme cells using knock-in embryos generated by ZFN-mediated gene addition, and found that there was variations in expression levels among primary mesenchyme cells dependent on the changes of cell-cell interaction.
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Report
(4 results)
Research Products
(126 results)
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[Journal Article] HpSulf, a heparin sulfate 6-O-endosulfatase, is involved in the regulation of VEGF signaling during seaurchin development2009
Author(s)
Fujita K, Takechi E, Sakamoto N, SumiyoshiN, Izumi S, Miyamoto T, Matsuura S, Tsurugaya T, Akasaka K, Yamamoto T.
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Journal Title
Mechanisms of Development
Volume: 127
Pages: 235-245
Related Report
Peer Reviewed
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