Molecular and Cellular mechanisms of germline mother cell regeneration in colonial tunicates

• Project/Area Number: 21570227
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Kochi University
• Principal Investigator: KAWAMURA Kazuo 高知大学, 教育研究部・自然科学系, 教授 (30136361)
• Co-Investigator(Kenkyū-buntansha): SUNANAGA Takeshi 高知大学, 教育研究部・自然科学系, 講師 (20448393)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 幹細胞 / 生殖細胞 / 再生 / 無性生殖 / ホヤ / 生殖系列幹細胞 / Vasa / Piwi / Myc / RACK1 / 群体ボヤ / NCR / 生殖系列 / non-coding RNA / キメラ

Research Abstract

Ascidians are the marine invertebrate that is most closely related to vertebrates. Colonial tunicates propagate by means of both sexual and asexual reproduction, and they can regenerate gonads and germ cells even though gonad components are completely extirpated from the colony. This study has disclosed that Myc/ Piwi-expressing cells are reserved in the hemocoel, and that they respond to BMP to express germline-specific gene Vasa, enabling the regeneration of germline cells and tissues.

Research Products

• [Journal Article] Tunicate cytostatic factor TC14 induces the polycomb group gene Eed and histone modification through Ca^<2+> binding and protein dimerization (2012)
  • Author(s): Kawamura, K., Takakura, K., Mori, D., Ikeda, K., Nakamura, A. and Suzuki, T.
  • Journal Title: BMC Cell Biol Volume: 13 Pages: 1-13
  • Peer Reviewed
• [Journal Article] Tunicate cytostatic factor TC14-3 induces a polycomb group gene and histone modification through Ca2+ binding and protein dimerization (2012)
  • Author(s): Kawamura, K., Takakura, K., Mori, D., Ikeda, K., Nakamura, A., Suzuki, T.
  • Journal Title: BMC Cell.Biol. Volume: 13 Issue: 1 Pages: 1-13
  • DOI: 10.1186/1471-2121-13-3
  • Peer Reviewed
• [Journal Article] Expression and function of myc during asexual reproduction of the budding ascidian, Polyandrocarpa misakiensis (2011)
  • Author(s): Fujiwara, S., Isozaki, T., Mori, K. and Kawamura, K.
  • Journal Title: Develop. Growth Differ Volume: 53 Pages: 1004-1014
  • NAID: 10030404228
  • Peer Reviewed
• [Journal Article] Role of Vasa, Piwi and Myc-expressing coelomic cells in gonad regeneration of the colonial tunicate (2011)
  • Author(s): Kawamura, K. and Sunanaga, T
  • Journal Title: Botryllus primigenus. Mech. Dev. Volume: 128 Pages: 457-470
  • Peer Reviewed
• [Journal Article] Germline cell formation and gonad regeneration in solitary and colonial ascidians (2011)
  • Author(s): Kawamura, K., Tiozzo, S., Manni, L., Sunanaga, T., Burighel, P. and De Tomaso A. W
  • Journal Title: Dev. Dyn Volume: 240 Pages: 299-308
  • Peer Reviewed
• [Journal Article] Germline cell formation and gonad regeneration in solitary and colonial ascidians (2011)
  • Author(s): Kawamura, K., Tiozzo, S., Manni, L., Sunanaga, T., Burighel, P., DeTomaso, A.
  • Journal Title: Dev.Dyn. Volume: 240 Issue: 2 Pages: 299-308
  • DOI: 10.1002/dvdy.22542
  • Peer Reviewed
• [Journal Article] Role of Vasa, Piwi, and Myc-expressing cells in gonad regeneration of the colonial tunicate, Botryllus primigenus (2011)
  • Author(s): Kawamura, K., Sunanaga, T.
  • Journal Title: Mech.Dev. Volume: 128 Issue: 7-10 Pages: 457-470
  • DOI: 10.1016/j.mod.2011.09.001
  • Peer Reviewed
• [Journal Article] Expression and function of myc during asexual reproduction of the budding ascidian, Polyandrocarpa misakiensis (2011)
  • Author(s): Fujiwara, S., Isozaki, T., Mori, K., Kawamura, K.
  • Journal Title: Dev.Growth Differ. Volume: 53 Issue: 9 Pages: 1004-1014
  • DOI: 10.1111/j.1440-169x.2011.01312.x
  • NAID: 10030404228
  • Peer Reviewed
• [Journal Article] Germline cell formation and gonad regeneration in solitary and colonial ascidians. (2011)
  • Author(s): Kawamura, K., Tiozzo, S., Manni, L., Sunanaga, T., Burighel, P., DeTomaso, A.
  • Journal Title: Dev.Dyn. Volume: 240 Pages: 299-308
  • Peer Reviewed
• [Journal Article] Hemoblasts in colonial tunicates : Are they stem cells or tissue-restricted progenitor cells? Develop (2010)
  • Author(s): Kawamura, K. and Sunanaga, T
  • Journal Title: Growth Differ Volume: 52 Pages: 69-76
  • Peer Reviewed
• [Journal Article] Regeneration of the gut requires retinoic acid in the budding ascidian Polyandrocarpa misakiensis (2010)
  • Author(s): Kaneko, N., Katsuyama, Y., Kawamura, K. and Fujiwara, S.
  • Journal Title: Develop. Growth Differ Volume: 52 Pages: 457-468
  • NAID: 10027565188
  • Peer Reviewed
• [Journal Article] Piwi-expressing hemoblasts serve as germline stem cells during postembryonic germ cell specification in colonial ascidian (2010)
  • Author(s): Sunanaga, T., Inubushi, H. and Kawamura, K.
  • Journal Title: Botryllus primigenus. Develop. Growth Differ Volume: 52 Pages: 603-614
  • Peer Reviewed
• [Journal Article] Hemoblasts in colonial tunicates : Are they stem cells or tissue-restricted progenitor cells? (2010)
  • Author(s): Kawamura, K., Sunanaga, T.
  • Journal Title: Dev.Growth Differ. Volume: 52 Pages: 69-76
  • NAID: 10027563115
  • Peer Reviewed
• [Journal Article] Regeneration of the gut requires retinoic acid in the budding ascidian Polyandrocarpa misakiensis. (2010)
  • Author(s): Kaneko, N., Katsuyama, Y., Kawamura, K., Fujiwara, S.
  • Journal Title: Dev.Growth Differ. Volume: 52 Pages: 457-468
  • NAID: 10027565188
  • Peer Reviewed
• [Journal Article] Piwi-expressing hemoblasts serve as germline stem cells during postembryonic germ cell specification in colonial ascidian, Botryllus primigenus. (2010)
  • Author(s): Sunanaga, T., Inubushi, H., Kawamura, K.
  • Journal Title: Dev.Growth Differ. Volume: 52 Pages: 603-614
  • NAID: 10027566047
  • Peer Reviewed
• [Journal Article] Hemoblasts in colonial tunicates : Are they stem cells or tissue-restricted progenitor cells? (2010)
  • Author(s): Kawamura, K.
  • Journal Title: Develop.Growth Differ. 52 Pages: 69-76
  • Peer Reviewed
• [Journal Article] Regeneration of the gut requires retinoic acid in the budding ascidian Polyandrocarpa misakiensis (2010)
  • Author(s): Kaneko, N.
  • Journal Title: Develop.Growth Differ 52(印刷中)
  • Peer Reviewed
• [Journal Article] The hemocytes of Polyandrocarpa misakiensis : morphology and immune-related activities (2009)
  • Author(s): Ballarin, L. and Kawamura, K
  • Journal Title: Invert. Surv. J. Volume: 6 Pages: 154-161
  • Peer Reviewed
• [Journal Article] The hemocytes of Polyandrocarpa misakiensis : morphology and immune-related activities (2009)
  • Author(s): Ballarin, L.
  • Journal Title: Invert.Surv.J. 6 Pages: 154-161
  • Peer Reviewed
• [Journal Article] RACK1 regulates mesenchymal cell recruitment during sexual and asexual reproduction in budding tunicates
  • Author(s): Tatzuke, Y., Sunanaga, T., Fujiwara, S. and Kawamura, K.
  • Journal Title: Dev. Biol
  • Peer Reviewed
• [Journal Article] Looking for putative phenoloxidases of compound ascidians : haemocyanin-like proteins in Polyandrocarpa misakiensis and Botryllus schlosseri
  • Author(s): Ballarin, L., Franchi, N., Schiavon, F., Tosatto, S. C. E, Mi. eti., I. and Kawamura, K.
  • Journal Title: Dev. Comp. Immunol.
  • Peer Reviewed
• [Presentation] Isolation and spatiotemporal expression analysis of SoxB1 and SoxB2 in colonial ascidian, Botryllus primigenus (2010)
  • Author(s): Kitamura, M.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートピア
  • Year and Date: 2010-12-08
• [Presentation] Cellular and molecular basis of germ cell specification in colonial ascidian, Botryllus primigenus (2010)
  • Author(s): Kashiwase, Y
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートピア
  • Year and Date: 2010-12-08
• [Presentation] Isolation and spatiotemporal expression analysis of SoxB1 and SoxB2 in colonial ascidian, Botryllus primigenus. (2010)
  • Author(s): Kitamura, M., Kawamura, K., Sunanaga, T.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] Cellular and molecular basis of germ cell specification in colonial ascidian, Botryllus primigenus. (2010)
  • Author(s): Kashiwase, Y., Tashiro, M., Kawamura, K., Sunanaga, T.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] Exploration of origin of germ cells in colonial ascidian Botryllus primigenus (2009)
  • Author(s): Sunanaga, T
  • Place of Presentation: 那覇市沖縄産業支援センター
  • Year and Date: 2009-06-24
• [Presentation] Regeneration of the gut requires retinoic acid in the budding ascidian Polyandrocarpa misakiensis (2009)
  • Author(s): Fujiwara, S
  • Place of Presentation: 那覇市沖縄産業支援センター
  • Year and Date: 2009-06-24
• [Presentation] Cellular and molecular basis of germ cell regeneration in colonial tunicates (2009)
  • Author(s): Kawamura, K
  • Organizer: The 5^<th> International Tunicate Meeting
  • Place of Presentation: 那覇市沖縄産業支援センター
  • Year and Date: 2009-06-22
• [Presentation] Role of RACK1 homologue in zooidal growth and reproduction of budding tunicates (2009)
  • Author(s): Kawamura, K
  • Organizer: The 5^<th> International Tunicate Meeting
  • Place of Presentation: 那覇市沖縄産業支援センター
  • Year and Date: 2009-06-22
• [Presentation] Exploration of origin of germ cells in colonial ascidian, Botryllus primigenus (2009)
  • Author(s): Sunanaga, T.
  • Organizer: The 5^<th> International Tunicate Meeting
  • Place of Presentation: 那覇市沖縄産業支援センター
• [Presentation] Regeneration of the gut requires retinoic acid in the budding ascidian Polyandrocarpa misakiensis (2009)
  • Author(s): Fujiwara, S.
  • Organizer: The 5^<th> International Tunicate Meeting
  • Place of Presentation: 那覇市沖縄産業支援センター