• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of the transition mechanism from mitosis to meiosis on spermatogenic cells by using cybrid model.

Research Project

Project/Area Number 21570228
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionYamaguchi University (2010-2011)
Nagasaki University (2009)

Principal Investigator

SATO Yoko  山口大学, 農学部, 学術研究員 (50398963)

Co-Investigator(Kenkyū-buntansha) KOJI Takehiko  長崎大学, 大学院・医歯薬学総合研究科, 教授 (30170179)
小路 武彦  長崎大学, 大学院・医歯薬学総合研究科, 教授 (30170129)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords精子形成 / 減数分裂 / 細胞培養系 / 細胞融合 / 培養系 / 発生・分化 / 細胞間橋 / 共培養系
Research Abstract

Meiosis is the special option for germ line cells to determine the cell fate. The aim of this study is to know the factors, which are involved in the induction or inhibition of meiosis in the spermatogonia. In the beginning, we tried to establish the experimental condition to make hetero karyotic cell by electronic fusion technique using both the competent spermatogenic cells, which can show meiotic progression, and the non-competent spermatogenic cells. However, the competent spermatogenic cells could not induce meiosis in the non-competent spermatogenic cells under hetero karyotic cell condition. To remove the effects of the donor nuclei from the recipient cells, cytoplasts(enucleated cytoplasm) from the competent spermatogenic cells were electro-fused with non-competent spermatogenic cells. The nuclei of the produced cybrids(cytoplasmic hybrid) were analyzed morphologically. These cybrids showed morphological changes in the nuclei similar to zygotene spermatocytes immediately after fusion. These results suggested that the cytoplasm of the competent spermatogenic cells contain the factor, which can induce meiotic progression in the non-competent spermatogenic cells. Further analysis including the expression of the specific molecular marker of the meiosis will need to be taken.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (52 results)

All 2012 2011 2010 2009 Other

All Journal Article (32 results) (of which Peer Reviewed: 32 results) Presentation (18 results) Book (2 results)

  • [Journal Article] Long-term preservation of chilled canine semen using vitamine C in combination with green tea polyphenol2012

    • Author(s)
      M. Wittayarat, K. Taichi, R. Kodama, Z. Namula, K. Chatdarong, M. Techakumphu, Y. Sato, M. Tachiguchi, T. Otoi
    • Journal Title

      Cryo Letters

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of sericin on preimplantation development of bovine embryos cultured individually2012

    • Author(s)
      T. Isobe, Y. Ikebata, T. Onitsuka, M. Wittayarat, Y. Sato, M. Taniguchi and T. Otoi.
    • Journal Title

      Theiogenology

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of(-)-epigallocatechin gallate on the motility and penetrability of frozen-thawed boar spermatozoa incubated in the fertilization medium2012

    • Author(s)
      Y. Kaedei, M. Naito, H. Naoi, Y. Sato, M. Taniguchi, F. Tanihara, K. Kikuchi, T. Nagai, and T. Otoi
    • Journal Title

      Reprod. Dom. Anim

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Glycoconjugates recognized by peanut agglutinin lectin in the inner acellular layer of the lamina propria of seminiferous tubules in human testes showing impaired spermatogenesis.2012

    • Author(s)
      Y. Sato, S. Nozawa, M. Yoshiike, T. Otoi and T. Iwamoto
    • Journal Title

      Hum. Reprod

      Volume: 27 Pages: 659-668

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gene suppression of mouse testis in vivo using small interfering RNA derived from plasmid vectors.2012

    • Author(s)
      T. Takizawa, T. Ishikawa, T. Kosuge, Y. Mizuguchi, Y. Sato, T. Koji, Y. Araki and T. Takizawa
    • Journal Title

      Acta Histochem. Cytochem

      Volume: 45 Pages: 77-81

    • NAID

      130001854310

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Estrogen-dependent regulation of sodium/ hydrogen exchanger-3(NHE3) expression via estrogen receptor B in proximal colon of pregnant mice2012

    • Author(s)
      N. Choijookhuu, Y. Sato, T. Nishino, D. Endo, Y. Hishikawa and T. Koji
    • Journal Title

      Histochem. Cell Biol

      Volume: 137 Pages: 575-587

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Follicle formation in the canine ovary after autografting to a peripheral site2012

    • Author(s)
      T. Terazono, Y. Kaedei, F. Tanihara, Z. Namura, V. L. Viet, M. Takagi, M. Inoue, Y. Sato, M. Taniguchi and T. Otoi
    • Journal Title

      Reprod. Domest. Anim

      Volume: 47 Pages: 16-21

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of (-)-epigallocatechin gallate on the motility and penetrability of frozen-thawed boar spermatozoa incubated in the fertilization medium.2012

    • Author(s)
      Y. Kaedei, M. Naito, H. Naoi, Y. Sato, M. Taniguchi, F. Tanihara, K. Kikuchi, T. Nagai, T. Otoi
    • Journal Title

      Reprod. Domest. Anim.

      Volume: 47(6) Issue: 6 Pages: 880-886

    • DOI

      10.1111/j.1439-0531.2012.01984.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gene suppression of mouse testis in vivo vising small interfering RNA derived from plasmid vectors2012

    • Author(s)
      T.Takizawa, T.Ishikawa, T.Kosuge, Y.Mizuguchi, Y.Sato, T.Koji, Y.Araki, T.Takizawa
    • Journal Title

      Acta Histochem.Cytochem.

      Volume: 45 Pages: 77-81

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Glycoconjugates recognized by peanut agglutinin lectin in the inner acellular layer of the lamina propria of seminiferous tubules in human testes showing impaired spermatogenesis.2012

    • Author(s)
      Y. Sato, S. Nozawa, M. Yoshiike, T. Otoi, T. Iwamoto
    • Journal Title

      Hum. Reprod

      Volume: 27 (6) Issue: 3 Pages: 659-668

    • DOI

      10.1093/humrep/der430

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Follicle formation in the canine ovary after autografting to a peripheral site.2012

    • Author(s)
      T. Terazono, Y. Kaedei, F. Tanihara, Z. Namura, V. L. Viet, M. Takagi, M. Inoue, Y. Sato, M. Taniguchi and T. Otoi
    • Journal Title

      Reprod. Domest. Anim.

      Volume: 47 (2) Issue: 2 Pages: 16-21

    • DOI

      10.1111/j.1439-0531.2011.01880.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Estrogen-dependent regulation of sodium/hydrogen exchanger-3 (NHE3) expression via estrogen receptor β in proximal colon of pregnant mice2012

    • Author(s)
      Choijookhuu N, Sato Y, Nishino T, Endo D, Hishikawa Y, Koji T
    • Journal Title

      Histochem.Cell Biol.

      Volume: 137 Issue: 5 Pages: 575-587

    • DOI

      10.1007/s00418-012-0935-2

    • NAID

      120006985370

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Long-temr preservation of chilled canine semen usin vitamine C in combination with green tea polyphenol2012

    • Author(s)
      M.Wittayarat, K.Taichi, R.Kodama, Z.Namula, K.Chatdarong, M.Techakum Y.Sato, M.Tachiguchi, T.Otoi
    • Journal Title

      Cryo Letters

      Volume: (in press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development and subsequent cryotolerance of domestic cat embryos cultured in serum-free and serum-containing media2011

    • Author(s)
      M. Murakami, Y. J. Dong, T. Suzuki, M. Taniguchi, Y. Kaedei, Y. Sato, F. Tanihara and T. Otoi
    • Journal Title

      Cryobiology

      Volume: 63 Pages: 170-174

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of cryoprotectant agents and equilibration methods on developmental competence of porcine oocytes2011

    • Author(s)
      M. Taniguchi, R. Arikawa, Y. Kaedei, F. Tanihara, Z. Namula, V. L. Viet, Y. Sato and T. Otoi
    • Journal Title

      Cryo Letters

      Volume: 32 Pages: 410-414

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development and subsequent cryotolerance of domestic cat embryos cultured in serum-free and serum-containing media2011

    • Author(s)
      M.Murakami, Y.J.Dong, T.Suzuki, M.Taniguchi, Y.Kaedei, Y.Sato, F.Tanihara, T.Otoi
    • Journal Title

      Cryo Biology

      Volume: 63 Issue: 3 Pages: 170-174

    • DOI

      10.1016/j.cryobiol.2011.06.002

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Xenografting of testicular tissue from an infant human donor results in accelerated testicular maturation2010

    • Author(s)
      Y. Sato, S. Nozawa, M. Yoshiike, M. Arai, C. Sasaki and T. Iwamoto
    • Journal Title

      Human Reprod

      Volume: 25 Pages: 1113-1122

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Xenografting of testicular tissue from an infant human donor results in accelerated testicular maturation.2010

    • Author(s)
      Sato, Y.
    • Journal Title

      Human Reproduction

      Volume: 25 Pages: 1113-1122

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pathogenesis of middle ear cholesteatoma ; a new model of experimentally induced cholesteatoma in Mongolian gerbils.2010

    • Author(s)
      Yamamoto-Fukuda T.
    • Journal Title

      American Journal of Pathology

      Volume: 176 Pages: 2602-2606

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of keratinocyte growth factor and its receptor in rat tracheal cartilage : Possible involvement in wound healing of the damaged cartilage.2010

    • Author(s)
      Abo, T.
    • Journal Title

      Acta Histochem Cytochem

      Volume: 43 Pages: 89-98

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Localization of HSP47 mRNA in murine bleomycin-induced pulmonary fibrosis.2010

    • Author(s)
      Kakugawa, T.
    • Journal Title

      Virchows Arch. 456

      Pages: 309-315

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aberrant expression of interferon regulatory factor 3 in human lung cancer.2010

    • Author(s)
      Tokunaga, T.
    • Journal Title

      Cancer Sci. (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of keratinocyte growth factor and its receptor in rat tracheal cartilage : Possible involvement in wound healing of the damaged cartilage.2010

    • Author(s)
      Abo, T.
    • Journal Title

      Acta Histochem. Cytochem. (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pathogenesis of middle ear cholesteatoma ; a new model of experimentally induced cholesteatoma in Mongolian gerbils.2010

    • Author(s)
      Yamamoto-Fukuda, T.
    • Journal Title

      Am.J.Pathol. (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Xenografting of testicular tissue from an infant human donor results in accelerated testicular maturation.2010

    • Author(s)
      Sato, Y.
    • Journal Title

      (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Glutathione S-transferase pi localizes in mitochondria and protects against oxidative stress.2009

    • Author(s)
      Goto, S
    • Journal Title

      Free Radic.Biol.Med. 46

      Pages: 1392-1403

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of keratinocyte growth factor and its receptor in odontogenic keratocysts.2009

    • Author(s)
      Suyama, Y.
    • Journal Title

      J.Oral.Pathol.Med. 38

      Pages: 476-480

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Keratinocyte growth factor accelerates compensatory growth in the remaining lung after trilobectomy in rats.2009

    • Author(s)
      Matsumoto, K.
    • Journal Title

      J.Thorac.Cardiovasc.Surg. 137

      Pages: 1499-1507

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Improvement of in situ PCR by optimization of PCR cycle number and proteinase k concentration : localization of x chromosome-linked phosphoglycerate kinase-1 gene in mouse reproductive organs.2009

    • Author(s)
      Hishikawa Y.
    • Journal Title

      Acta Histochem. Cytochem. 42

      Pages: 15-21

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Human Papillomavirus infection and its possible correlation with p63 expression in cervical cancer among Japan, Mongolia and Myanmar.2009

    • Author(s)
      Ulziibat, S.
    • Journal Title

      Acta Histochem. Cytochem. 42

      Pages: 181-190

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diverse effects of FK506 on the apoptosis of hepatocytes and infiltrating lymphocytes in an allografted rat liver.2009

    • Author(s)
      Moriuchi, H.
    • Journal Title

      J Surg. Res. (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Studying spermatogenesis by using in vivo and in vitro models : Advantages and disadvantages of these models for practical use

    • Author(s)
      Y. Sato, M. Taniguchi, T. Otoi
    • Journal Title

      J. Vet. Sci. Tech

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Presentation] Application of in vitro spermatogenesis model2011

    • Author(s)
      Y. Sato
    • Organizer
      JICA program for Southeast Asia
    • Place of Presentation
      Yamaguchi University, Yamaguchi
    • Year and Date
      2011-10-11
    • Related Report
      2011 Final Research Report
  • [Presentation] Application of in vitro spermatogenesis model2011

    • Author(s)
      佐藤陽子
    • Organizer
      JICA program for Southeast Asia
    • Place of Presentation
      山口大学(山口市)
    • Year and Date
      2011-10-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] 造精機能障害を示す精巣について2011

    • Author(s)
      佐藤陽子
    • Organizer
      脂質栄養と性差に関するオープンリサーチセンター整備事業講演会
    • Place of Presentation
      金城学院大学,名古屋
    • Year and Date
      2011-08-19
    • Related Report
      2011 Final Research Report
  • [Presentation] 造精機能障害を示す精巣について2011

    • Author(s)
      佐藤陽子
    • Organizer
      脂質栄養と性差に関するオープンリサーチセンター講演会
    • Place of Presentation
      金城学院大学(名古屋市)
    • Year and Date
      2011-08-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] マウス精子形成過程における5-aza-2'一deoxycytidine(5-aza-dC)のエピジェネティック因子への効果2011

    • Author(s)
      宋寧
    • Organizer
      第88回日本生理学会大会/第116回日本解剖学会総会・全国学術集会合同大会
    • Place of Presentation
      横浜パシフィコ横浜(震災により紙上開催)
    • Year and Date
      2011-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] Apoptosis related distribution of mitochondrial components assessed by fluorescence-immunohistochemistry with laser scanning microscopy in mouse spermatogenic cells.2011

    • Author(s)
      Hishikawa, Y.
    • Organizer
      Myanmar Health Research Congress
    • Place of Presentation
      Yangon, Myanmar
    • Year and Date
      2011-01-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Molecular histochemical analysis of DNA methylation status of specific DNA sequences by histo endonuclease-linked detection of methylation sites of DNA(HELMET).2010

    • Author(s)
      Koji, T.
    • Organizer
      The 6th Asian Science Seminar
    • Place of Presentation
      Taichung, Taiwan
    • Year and Date
      2010-11-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 雄性生殖細胞におけるpiRNAのlive imaging解析2010

    • Author(s)
      佐藤陽子
    • Organizer
      組織的な若手研究者等海外派遣プログラムシンポジウム
    • Place of Presentation
      長崎良順会館
    • Year and Date
      2010-11-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] Association between carcinogenic effect of Aflatoxin B 11(AFB1)and other histochemical markers on Hepatocellular Carcinoma.2010

    • Author(s)
      Yin Min Htun
    • Organizer
      Myanmar Health Research Congress 2010
    • Place of Presentation
      Yangon, Myanmar
    • Year and Date
      2010-09-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] HELMET法によるDNAメチル化レベルの解析と精子形成過程に於けるその変動2010

    • Author(s)
      小路武彦
    • Organizer
      第51回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      東京秋葉原コンベンションホール
    • Year and Date
      2010-09-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] Effect of 5-aza-2'-deoxycytidine(5-aza-dC) on epigenetic modifications in spermatogenic cells of mouse2010

    • Author(s)
      N. Song, T. Nishino, Y. Sato, Y. Hishikawa, T. Koji
    • Organizer
      第51回日本組織細胞化学会総会
    • Place of Presentation
      秋葉原コンベンションセンター,東京
    • Year and Date
      2010-09-04
    • Related Report
      2011 Final Research Report
  • [Presentation] Effect of 5-aza-2 deoxycytidine(5-aza-dC)on epigenetic modifications in spermatogenic cells of mouse.2010

    • Author(s)
      Song, N.
    • Organizer
      第51回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      東京秋葉原コンベンションホール
    • Year and Date
      2010-09-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] Nucleolar localization of rDNA transcripts detected by electron microscopical insitu hybridization.2009

    • Author(s)
      小路武彦
    • Organizer
      The 9^<th>China-Japan Joint Seminar on Histochemistry and Cytochemistry
    • Place of Presentation
      Nanning, China
    • Year and Date
      2009-11-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] Differentiation stage-specific changes of DNA methylation and histone H3 modifications in mouse spermatogenesis.2009

    • Author(s)
      宋寧
    • Organizer
      第50回日本組織細胞化学会・学術集会
    • Place of Presentation
      滋賀県大津市
    • Year and Date
      2009-09-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] Differentiation stage-specific changes of DNA methylation and histone H3 modifications in mouse spermatogenesis2009

    • Author(s)
      N. Song, Y. Hishitaka, Y. Sato, T. Koji
    • Organizer
      第50回日本組織細胞化学会総会
    • Place of Presentation
      ピアザ淡海,大津
    • Year and Date
      2009-09-26
    • Related Report
      2011 Final Research Report
  • [Presentation] 生殖細胞に発現しているTex101に関するin vivoノックダウン解析の試み2009

    • Author(s)
      瀧澤敬美・石川朋子・石橋宰・後藤忠・佐藤陽子・小路武彦・荒木慶彦・瀧澤俊広
    • Organizer
      第50回日本組織細胞化学会総会
    • Place of Presentation
      ピアザ淡海,大津
    • Year and Date
      2009-09-26
    • Related Report
      2011 Final Research Report
  • [Presentation] DNAメチル化部位局在のための新しい組織化学 : HELMET法2009

    • Author(s)
      小路武彦
    • Organizer
      第41回日本臨床分子形態学会総会・学術集会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2009-09-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] マウス生殖細胞アポトーシス誘導におけるミトコンドリア動態とERβ発現の関与2009

    • Author(s)
      菱川善隆・安樹才・荒木慶彦・松田賢一・河田光博・小路武彦
    • Organizer
      第114回日本解剖学会総会
    • Place of Presentation
      岡山理科大学,岡山
    • Year and Date
      2009-03-29
    • Related Report
      2011 Final Research Report
  • [Book] 組織細胞化学2010(日本組織細胞化学会編)(転写調節解析への組織化学的アプローチ:転写因子とDNAメチル化部位の局在証明法)2010

    • Author(s)
      小路武彦
    • Total Pages
      10
    • Publisher
      中西印刷
    • Related Report
      2010 Annual Research Report
  • [Book] 永遠の不死-精子形成細胞の生物学-[新・生命科学ライブラリー医学とバイオ6]2009

    • Author(s)
      小路武彦
    • Total Pages
      139
    • Publisher
      サイエンス社
    • Related Report
      2009 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi