Localization mechanisms of axon guidance molecules and their receptors in C. elegans

• Project/Area Number: 21570230
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Yokohama City University
• Principal Investigator: OGURA Ken-ichi 横浜市立大学, 医学部, 助教 (20326028)
• Co-Investigator(Renkei-kenkyūsha): GOSHIMA Yoshio 横浜市立大学, 医学研究科, 教授 (00153750)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 発生遺伝 / 軸索ガイダンス / C.elegans / Netrin / 線虫 / UNC-5 / 局在機構 / 神経 / C. elegans

Research Abstract

A hypothesis that localizations of axon guidance molecules and their receptors cause variability of neural networks is proposed. However, the molecular mechanisms are largely unknown. By using a model organism C. elegans, we found molecules that regulate localization of an axon guidance molecule UNC-6/Netrin and its receptor UNC-5.

Research Products

• [Journal Article] Localization mechanisms of the axon guidance molecule UNC-6/Netrin and its receptors (2012)
  • Author(s): Ogura K, Asakura T, Goshima Y.
  • Journal Title: Caenorhabditis elegans Volume: 54(3) Issue: 3 Pages: 390-7
  • DOI: 10.1111/j.1440-169x.2012.01349.x
  • Peer Reviewed
• [Journal Article] A seven-transmembrane receptor that mediates avoidance response to dihydrocaffeic acid, a water-soluble repellent in Caenorhabditis elegans (2011)
  • Author(s): Aoki R, Yagami T, Sasakura H, Ogura K, Kajihara Y, Ibi M, Miyamae T, Nakamura F, Asakura T, Kanai Y, Misu Y, Iino Y, Ezcurra M, Schafer WR, Mori I, Goshima Y. J Neurosci.
  • Journal Title: J Neurosci Volume: 31(46) Pages: 16603-10
  • Peer Reviewed
• [Journal Article] Mutation in a mitochondrial ribosomal protein causes increased sensitivity to oxygen with decreased longevity in the nematode Caenorhabditis elegans (2011)
  • Author(s): Fujii M, Shikatani K, Ogura K, Goshima Y, Ayusawa D.
  • Journal Title: Genes Cells Volume: 16(1) Issue: 1 Pages: 69-79
  • DOI: 10.1111/j.1365-2443.2010.01469.x
  • Peer Reviewed
• [Journal Article] A seven-transmembrane receptor that mediates avoidance response to dihydrocaffeic acid, a water-soluble repellent in Caenorhabditis elegans (2011)
  • Author(s): 小倉顕一
  • Journal Title: J Neurosci Volume: 31 Pages: 16603-16610
  • Peer Reviewed
• [Journal Article] Mutation in a mitochondrial ribosomal protein causes increased sensitivity to oxygen with decreased longevity in the nematode Caenorhabditis elegans. (2011)
  • Author(s): 小倉顕一
  • Journal Title: Genes to Cells Volume: 16 Pages: 69-79
  • Peer Reviewed
• [Journal Article] Protein phosphatase 2A cooperates with the autophagy-related kinase UNC-51 to regulate axon guidance in Caenorhabditis elegans (2010)
  • Author(s): Ogura K, Okada T, Mitani S, Gengyo-Ando K, Baillie DL, Kohara Y, Goshima Y.
  • Journal Title: Development Volume: 137(10) Pages: 1657-67
  • Peer Reviewed
• [Journal Article] Genes required for cellular UNC-6/n etrin localization in Caenorhabditis elegans (2010)
  • Author(s): Asakura T, Waga N, Ogura K, Goshima Y.
  • Journal Title: Genetics Volume: 85(2) Pages: 573-85
  • Peer Reviewed
• [Journal Article] Genes required for cellular UNC-6/Netrin localization in Caenorhabditis elegans (2010)
  • Author(s): 小倉顕一
  • Journal Title: Genetics (in press)
  • Peer Reviewed
• [Journal Article] Protein phosphatase 2A cooperates with the autophagy-related kinase UNC-51 to regulate axon guidance in Caenorhabditis elegans (2010)
  • Author(s): 小倉顕一
  • Journal Title: Development 137 Pages: 1657-1667
  • Peer Reviewed
• [Presentation] The secretory mechanism of an axon guidance molecule UNC-6/Netrin (2011)
  • Author(s): Wataru Okubo, Taro Asakura, Kumiko Fujita, Kazunori Hanaoka, Yoshio Goshima, Ken-ichi Ogura
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-16
• [Presentation] A vesicular transport mechanism regulates UNC-6/Netrin (2011)
  • Author(s): Taro Asakura, Ken-ichi Ogura, Kumiko Fujita, Yoshio Goshima
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-16
• [Presentation] The secretory mechanism of an axon guidance molecule UNC-6/Netrin (2011)
  • Author(s): 小倉顕一
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-16
• [Presentation] A copine like protein CPNA-2 regulates neural localization of UNC-6/Netrin in Caenorhabditis elegans (2011)
  • Author(s): Taro Asakura, Kumiko Fujita, Yoshio Goshima and Ken-ichi Ogura
  • Organizer: 18th International C. elegans Meeting
  • Place of Presentation: UCLA
  • Year and Date: 2011-06-24
• [Presentation] Isolation and characterization of unc-51 suppressor mutants in C. elegans (2010)
  • Author(s): Ken-ichi Ogura, Hiroshi Matsuoka and Yoshio Goshima
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] UNC-104/KIF1A regulates the transport of UNC-6/Netrin in neurons (2010)
  • Author(s): Taro Asakura, Kumiko Fujita, Yoshio Goshima and Ken-ichi Ogura
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] Isolation and characterization of unc-51 suppressor mutants in C.elegans (2010)
  • Author(s): 小倉顕一
  • Organizer: 第33回日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド(兵庫)
  • Year and Date: 2010-12-08
• [Presentation] 線虫C.elegans の筋肉形成異常変異unc-22、unc-15はunc-51変異体の軸索ガイダンス異常を抑制する (2009)
  • Author(s): 小倉顕一
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-12
• [Presentation] オートファジー関連分子Atg-4.2は軸索ガイダンス分子UNC-6/Netrin受容体UNC-5の細胞内局在を制御する (2009)
  • Author(s): 朝倉太郎, 小倉顕一, 和賀真子, 岡田貴子, 五嶋良郎
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] 線虫C. elegansの筋肉形成異常変異unc-22、unc-15はunc-51変異体の軸索ガイダンス異常を抑制する (2009)
  • Author(s): 小倉顕一、五嶋良郎
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] Genes required for localization of UNC-6/Netrin and/or UNC-5 in Caenorhabditis elegans (2009)
  • Author(s): 小倉顕一
  • Organizer: 17th International C.elegans Meeting
  • Place of Presentation: UCLA
  • Year and Date: 2009-06-27
• [Presentation] Genes required for localization of UNC-6/Netrin and/or UNC-5 in Caenorhabditis elegans (2009)
  • Author(s): Taro Asakura, Naoko Waga, Ken-ichi Ogura and Yoshio Goshima
  • Organizer: 17th International C. elegans Meeting
  • Place of Presentation: UCLA
  • Year and Date: 2009-06-26