Single cell analysis of tail regeneration in Xenopus tadpole

• Project/Area Number: 21570232
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: University of Hyogo
• Principal Investigator: MOCHII Makoto 兵庫県立大学, 大学院・生命理学研究科, 准教授 (90202358)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: アフリカツメガエル / 尾部再生 / 脊索 / 表皮 / 細胞分化 / 分化 / 液胞

Research Abstract

To analyze direct effects of fibroblast growth factor and hedgehog signalings in the tail regeneration notochord cells were isolated from Xenopus tadpole. Isolated immature cells proliferated and terminally differentiated into vacuole-containing cells in a culture condition. The vacuolation was inhibited by FGFs or by hedgehog inhibitor. Genes associated with the notochord maturation were identified after a gene expression screening.

Research Products

• [Journal Article] The Analysis of the Expression of a Novel Gene, Xenopus Polka Dots, which was Expressed in the Embryonic and Larval Epidermis during Early Development (2011)
  • Author(s): Yoshii S, Yamaguchi M, Oogata Y, Tazaki A, Mochii M, Suzuki S, Kinoshita T.
  • Journal Title: Zoolog Sci Volume: 28(11) Issue: 11 Pages: 809-16
  • DOI: 10.2108/zsj.28.809
  • NAID: 40019070956
  • Peer Reviewed
• [Journal Article] An overview of transcriptome profiles identified in hagfish, shark, and (2011)
  • Author(s): Takechi M, et al
  • Journal Title: J Exp Zool B Mol Dev Evol Volume: 316(7) Issue: 7 Pages: 526-546
  • DOI: 10.1002/jez.b.21427
  • Peer Reviewed
• [Journal Article] Germ-line mitochondria exhibit suppressed respiratory activity to support their accurate transmission to the next generation (2011)
  • Author(s): Kogo N, Tazaki A, Kashino Y, Morichika K, Orii H, Mochii M, Watanabe K.
  • Journal Title: Dev Biol Volume: 349 Issue: 2 Pages: 462-469
  • DOI: 10.1016/j.ydbio.2010.11.021
  • Peer Reviewed
• [Journal Article] Germ-line mitochondria exhibit suppressed respiratory activity to support their accurate transmission to the next generation. (2011)
  • Author(s): Kogo N, Tazaki A, Kashino Y, Morichika K, Orii H, Mochii M, Watanabe K.
  • Journal Title: Dev Biol. Volume: 349 Pages: 462-469
  • Peer Reviewed
• [Journal Article] Characterization of a novel type I keratin gene and generation of transgenic lines with fluorescent reporter genes driven by its promoter/enhancer in Xenopus laevis (2010)
  • Author(s): Suzuki, K, Kashiwagi, K, Ujihara, M, Marukane, T, Tazaki, A, Watanabe, K, Mizuno, N, Ueda, Y, Kondoh, H, Kashiwagi, A and Mochii, M
  • Journal Title: Dev Dyn Volume: 239 Issue: 12 Pages: 3172-3181
  • DOI: 10.1002/dvdy.22451
  • Peer Reviewed
• [Journal Article] C38, equivalent to BM88, is developmentally expressed in maturing retinal neurons and enhances neuronal maturation (2010)
  • Author(s): Wakabayashi, T, Kosaka, J, Mochii, M, Miki, Y, Mori, T, Takamori, Y and Yamada, H.
  • Journal Title: J. Neurochem Volume: 1112 Issue: 5 Pages: 1235-1248
  • DOI: 10.1111/j.1471-4159.2009.06536.x
  • Peer Reviewed
• [Journal Article] Perturbation of Notch/Suppressor of Hairless pathway disturbs migration of primordial germ cells in Xenopus embryo (2010)
  • Author(s): Morichika, K, Kataoka, K, Terayama, K, Tazaki, A, Kinoshita, T, Watanabe, K and Mochii, M.
  • Journal Title: Dev. Growth Differ Volume: 52 Pages: 235-244
  • NAID: 10027563960
  • Peer Reviewed
• [Journal Article] Characterization of a novel type I keratin gene and generation of transgenic lines with fluorescent reporter genes driven by its promoter/enhancer in Xenopus laevis. (2010)
  • Author(s): Suzuki, K, Kashiwagi, K, Ujihara, M, Marukane, T, Tazaki, A, Watanabe, K, Mizuno, N, Ueda, Y, Kondoh, H, Kashiwagi, A ,Mochii, M
  • Journal Title: Dev Dyn. Volume: 239 Pages: 3172-3181
  • Peer Reviewed
• [Journal Article] Schwann cell precursors from nerve innervation are a cellular origin of melanocytes in skin (2009)
  • Author(s): Adameyko I, Lallemend F, Aquino JB, Pereira JA, Topilko P, Muller T, Fritz N, Beljajeva A, Mochii M, Liste I, Usoskin D, Suter U, Birchmeier C and Ernfors P
  • Journal Title: Cell Volume: 139 Issue: 2 Pages: 366-379
  • DOI: 10.1016/j.cell.2009.07.049
  • Peer Reviewed
• [Journal Article] Xenopus Wnt-5a induces an ectopic larval tail at injured site, suggesting a crucial role for noncanonical Wnt signal in tail regeneration (2009)
  • Author(s): Sugiura, T, Tazaki, A, Ueno, N, Watanabe, K and Mochii, M
  • Journal Title: Mech. Dev Volume: 126 Issue: 1-2 Pages: 56-67
  • DOI: 10.1016/j.mod.2008.10.002
  • Peer Reviewed
• [Journal Article] Xenopus Wnt-5a induces an ectopic larval tail at injured site, suggesting a crucial role for noncanonical Wnt signal in tail regeneration (2009)
  • Author(s): Sugiura, T, Tazaki, A, Ueno, N, Watanabe, K, Mochii, M
  • Journal Title: Mech.Dev. 126 Pages: 56-67
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Makoto Mochii, Yuka Taniguchi
  • Journal Title: Electroporation in the regenerating tail of the Xenopus tadpole. In Electroporation and Sonoporation in Developmental Biology. (Ed.H.Nakamura)(Springer, Tokyo) Pages: 239-247
• [Journal Article] (2009)
  • Author(s): Makoto Mochii, Yuka Taniguchi
  • Journal Title: Electroporation in the regenerating tail of the Xenopus tadpole. In Electroporation and Sonoporation in Developmental Biology. (Ed.H.Nakamura)(Springer, Tokyo) Pages: 239-247
• [Presentation] Xenopus ES1 is expressed in the wound epidermis of regenerating tail and limb bud in tadpole (2012)
  • Author(s): Shigehiro Yasukawa, Kaname Yoneda, Akira Tazaki, Kenji Watanabe, Makoto Mochii
  • Organizer: Joint Meeting of the 45th Annual Meeting of the Japanese Society of Developmental Biologist & The 64th Annual Meeting of the Japan Society for Cell Biology
  • Place of Presentation: Kobe
• [Presentation] ゼノパス始原生殖細胞の運動制御機構 (2011)
  • Author(s): 餅井真
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川大雪クリスタルホール(北海道)(招待講演)
  • Year and Date: 2011-09-12
• [Presentation] Terminal differentiation of notochord cells isolated from Xenopus tadpole (2011)
  • Author(s): Mochii M, Watanabe K
  • Organizer: 第44回日本発生生物学会年会
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県)
  • Year and Date: 2011-05-20
• [Presentation] In vitro studies on migratory activity of Xenopus primordial germ cells (2011)
  • Author(s): Terayama K, Kataoka K, Morichika K, Orii H, Watanabe K, Mochii M
  • Organizer: 第44回日本発生生物学会年会
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県)
  • Year and Date: 2011-05-19
• [Presentation] アフリカツメガエル始原生殖細胞の移動に関する研究 (2011)
  • Author(s): 寺山耕平、片岡研介、森近恵祐、渡辺憲二、織井秀文、餅井真
  • Organizer: 日本動物学会82回大会
  • Place of Presentation: 旭川
• [Presentation] ゼノパス始原生殖細胞の運動制御機構 (2011)
  • Author(s): 餅井真
  • Organizer: 日本動物学会82回大会
  • Place of Presentation: 旭川
• [Presentation] Terminal differentiation of notochord cells isolated from Xenopus tadpole (2011)
  • Author(s): Makoto Mochii, Kenji Watanabe
  • Organizer: 44th Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Ginowan
• [Presentation] In vitro studies on migratory activity of Xenopus primordial cells (2011)
  • Author(s): Kohei Terayama, Kensuke Kataoka, Keisuke Morichika, Hidefumi Orii, Kenji Watanabe, Makoto Mochii
  • Organizer: 44th Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Ginowan
• [Presentation] In vitro study on terminal differentiation of notochord cells isolated from Xenopus tadpole (2011)
  • Author(s): Makoto Mochii, Kenji Watanabe
  • Organizer: Joint Meeting of the German and Japanese Societies of Developmental Biologists
  • Place of Presentation: Dresden
• [Presentation] In vitro study on terminal differentiation of notochord cells isolated from Xenopus tadpole (2011)
  • Author(s): Mochii, M., Watanabe, K
  • Organizer: Joint Meeting of the German and Japanese Societies of Developmental Biologists.
  • Place of Presentation: Dresden, Germany
• [Presentation] 生殖隆起に入らなかったXenopus始原生殖細胞におけるXtrの量的変化と形態変化 (2010)
  • Author(s): 杉本哲治, 日吉真照、片岡研介, 鮭真, 渡辺憲二, 高宗和史
  • Organizer: 日本動物学会81回大会
  • Place of Presentation: 東京
• [Presentation] Xenopus始原生殖細胞の運動性は発生時期に依存して変化する (2010)
  • Author(s): 寺山耕平、東野仁央、織井秀文、渡辺憲二、餅井真
  • Organizer: 日本動物学会81回大会
  • Place of Presentation: 東京
• [Presentation] Developmental change of motility in Xenopus PGCs (2010)
  • Author(s): Kohei Terayama, Kensuke Kataoka, Keisuke Morichika, Hidefumi Orii, Kenji Watanabe, Makoto Mochii
  • Organizer: 13th International Xenopus Conference
  • Place of Presentation: Lake Louise, Canada
• [Presentation] Fibroblast growth factor and hedgehog regulate growth and maturation of larval notochord cell in Xenopus (2010)
  • Author(s): Shunsuke Yoneda, Kenji Watanabe, Makoto Mochii
  • Organizer: CDB symposium
  • Place of Presentation: Kobe
• [Presentation] Fibroblast growth factor and hedgehog regulate growth and maturation of larval notochord cell in Xenopus (2010)
  • Author(s): Yoneda, S, Watanabe, K, Mochii, M
  • Organizer: CDB Symposium 2010 : Frontiers in Organogenesis
  • Place of Presentation: Kobe
• [Presentation] 生殖隆起に入れなかったXenopus始原生殖細胞におけるXtrタンパク質の消失と形態変化 (2009)
  • Author(s): 杉本哲治, 片岡研介, 餅井真, 渡辺憲二, 高宗和史
  • Organizer: 日本動物学会80回大会
  • Place of Presentation: 静岡
• [Presentation] アフリカツメガエルの初期原腸胚における生殖細胞質の再配置 (2009)
  • Author(s): 田口彩華、平林美紀、餅井真、渡辺憲二
  • Organizer: 日本動物学会80回大会
  • Place of Presentation: 静岡
• [Presentation] Molecular mechanism of primordial germ cell migration in Xenopus (2009)
  • Author(s): Kohei Terayama, Kensuke Kataoka, Keisuke Morichika, Kenji Watanabe, Hidefumi Orii, Makoto Mochii
  • Organizer: 42nd Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Niigata
• [Presentation] Characterization of Xpod-expressing cells in the Xenopus larval epidermis (2009)
  • Author(s): Shunsuke Yoshii, Masahiro Yamaguchi, Yuichiro Oogata, Akira Tazaki, Makoto Mochii, Shintaro Suzuki, Tsutomu Kinoshita
  • Organizer: 42nd Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Niigata
• [Presentation] Activity of mitochondria in germ plasm is regulated dramatically during oogenesis of Xenopus laevis (2009)
  • Author(s): Naomi Kogo, Akira Tazaki, Hidefumi Orii, Makoto Mochii, Kenji Watanabe
  • Organizer: 42nd Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Niigata
• [Presentation] In vivo and in vitro analysis of notochord cell differentiation of Xenopus larva (2009)
  • Author(s): Shunsuke Yoneda, Kenji Watanabe, Makoto Mochii
  • Organizer: 42nd Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Niigata
• [Presentation] In vivo and in vitro analysis of notochord cell differentiation of Xenopus larva (2009)
  • Author(s): Yoneda, S, Watanabe, K, Mochii, M
  • Organizer: 第42回日本発生生物学会年会
  • Place of Presentation: 新潟
• [Presentation] Characterization of Xpod-expressing cells in the Xenopus larval epidermis (2009)
  • Author(s): Yoshii, S, Yamaguchi, M, Oogata, Y, Tazaki, A, Mochii, M, Suzuki, S, Kinoshita, T
  • Organizer: 第42回日本発生生物学会年会
  • Place of Presentation: 新潟
• [Book] Electroporation in the regenerating tail of the Xenopus tadpole. In Electroporation and Sonoporation in Developmental Biology (2009)
  • Author(s): Makoto Mochii, Yuka Taniguchi
  • Publisher: Springer