A sperm motility-controlling protein adapted to the urodele mode of internal fertilization and its diversity among amphibian species
| Project/Area Number |
21570236
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Evolutionary biology
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| Research Institution | Yamagata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKAUCHI Yuni 山形大学, 理学部, 助教 (60250908)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 体内受精 / 精子運動 / 機能進化 / 両生類 / 輸卵管 |
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| Research Abstract |
Sperm motility-initiating substance is a nonglycosilated protein that acts specifically in the mode of internal fertilization of the urodele amphibian, Cynops pyrrhogaster. In the present study, a cDNA encoding SMIS protein was identified. It coded a sequence of 151 amino acids that contained a cysteine knot domain. The expression of the SMIS gene was oviduct-specific and independent of the stimuli of ovulation. The active site of the SMIS was detected in a hydrophilic loop of the cysteine knot domain using synthetic peptides having partial amino acid sequences of the SMIS. Immunoblotting and southernblotting revealed that proteins homologous to SMIS were widely conserved among anuran and urodele amphibians but those homologues showed different isoelectric points ranging from 4 to 7. These results suggest that the molecular feature of the SMIS may be changed in adaptation to the diversified fertilization modes among amphibian species.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Identification of the sperm motility-initiating substance in the newt, Cynops pyrrhogaster, and its possible relation with the acrosome reaction during internal fertilization2010
Author(s)
T. Watanabe, H. Kubo, S. Takeshima, M. Nakagawa, M. Ohta, S. Kamimura, E. Takayama-Watanabe, A. Watanabe, K. Onitake
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Journal Title
Int. J. Dev. Biol
Volume: 54
Pages: 591-597
Related Report
Peer Reviewed
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