Study on molecular co-evolution of enzyme and substrate
| Project/Area Number |
21570240
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Evolutionary biology
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| Research Institution | Sophia University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NISIDA Mutumi 東京大学, 海洋研究所, 教授 (90136896)
NAGATA Koji 東京大学, 大学院・農学生命科学研究科, 准教授 (30280788)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 機能進化 / 共進化 / 孵化酵素 / 遺伝子重複 / 卵膜 / 硬骨魚類 / 卵膜タンパク質 / 卵膜分解機構 / 新規機能遺伝子 / 遺伝子 / 進化 / 酵素 |
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| Research Abstract |
In previous phylogenetic analysis, we suggested that fishes included in Elopomorpha, as basal teleosts, possess a single type of hatching enzyme genes, and that fishes in Otocephala and Euteleostei gain two types of hatching enzyme genes called clade I and II genes by duplication. The Elopomorph hatching enzyme and the clade I enzyme swell the egg envelope by cleaving the N-terminal regions of ZP proteins, while the clade II enzyme solubilizes the swollen envelope by cleaving the two specific sites at N-terminal of ZP domain and the middle region on the ZP domain. In the evolutionary scenario, our findings support that the clade I enzymes inherit the ancestral enzyme function, and the clade II enzymes gain a new function during evolution to Otocephala and Euteleostei. Our study also suggested that a change in the egg envelope protein during evolution strongly contributed to the acquisition of a new function of euteleostean clade II enzyme.
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Report
(4 results)
Research Products
(36 results)
