| Project/Area Number |
21570242
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Evolutionary biology
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| Research Institution | The University of Tokyo (2010-2011)
National Institute of Advanced Industrial Science and Technology (2009) |
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Principal Investigator |
WATANABE Kimitsuna 東京大学, 大学院・農学生命科学研究科, 名誉教授 (00134502)
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| Co-Investigator(Kenkyū-buntansha) |
YOKOBORI Shin-ichi 東京薬科大学, 生命科学部, 講師 (40291702)
SHIGI Naoki (独)産業技術総合研究所, バイオメディシナル情報研究センター, 研究員 (20392623)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 遺伝暗号 / ミトコンドリア / tRNA / 修飾塩基 / 分子進化 / マボヤ / ヤリイカ / 非普遍暗号 / ゲノム解析 |
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| Research Abstract |
In animal mitochondria, distribution of the non-universal codons(relationship between codon and amino acid) has been clarified in all animal phyla. However, relationship between codon and anticodon has not clarified except for Vertebrata and Nematoda. In order to elucidate a whole image for the decoding mechanism of genetic code, we isolated the relevant tRNAs from a tunicate(ascidian) and a mollusc(squid) and analysed them by using LC/MS. By doing so, we clarified the relationship. We found 5-taurinomethyluridine(. m5U) in the first position of ascidian tRNATrp, tRNAMet and tRNAGly, and squid tRNATrp and concluded that. m5U-bearing anticodons decode A-and G-ending codons.
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