Development of TILLING platform for the elucidation of important breeding traits in tomato

• Project/Area Number: 21580004
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: University of Tsukuba
• Principal Investigator: EZURA Hiroshi 筑波大学, 生命環境系, 教授 (00332552)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: トマト / EMS / 変異体 / TILLING / 重要形質 / 日持ち性 / 重要形

Research Abstract

TILLING (Targeting Induced Local Lesions In Genomes) platform has been established in Micro-Tom cultivar, which is a model tomato cultivar for fruit biology. TILLING allows us to isolate mutants, which has mutations in gene of interests. Using the TILLING platform, ripening-related mutants were isolated and characterized, demonstrating the validity of the established platform.

Research Products

• [Journal Article] Availability of Micro-Tom mutant library combined with TILLING in molecular breeding of tomato fruit shelf-life (2012)
  • Author(s): Okabe Y, Asamizu E, Ariizumi T, Shirasawa K, Tabata S, Ezura H.
  • Journal Title: Breeding Science
  • NAID: 10030434826
  • Peer Reviewed
• [Journal Article] Availability of Micro-Tom mutant library combined with TILLING in molecular breeding of tomato fruit shelf-life (2012)
  • Author(s): Okabe Y, Asamizu E, Ariizumi T, Shirasawa K, Tabata S, Ezura H
  • Journal Title: Breeding Science Volume: (印刷中)
  • NAID: 10030434826
  • Peer Reviewed
• [Journal Article] Tomato TILLING technology : Development of a reverse genetics tool for the efficient isolation of mutants from Micro-Tom mutant libraries. (2011)
  • Author(s): Okabe Y, Asamizu E, Saito T, Matsukura C, Ariizumi T, Bres C, Rothan C, Mizoguchi T, Ezura H.
  • Journal Title: Plant and Cell Physiology Volume: 52(11)
  • NAID: 10030305636
  • Peer Reviewed
• [Journal Article] Tomato TILLING technology : Development of a reverse genetics tool for the efficient isolation of mutants from Micro-Tom mutant libraries (2011)
  • Author(s): Okabe Y, Asamizu E, Saito T, Matsukura C, Ariizumi T, Bres C, Rothan C, Mizoguchi T, Ezura H
  • Journal Title: Plant and Cell Physiology Volume: 52 Issue: 11 Pages: 1994-2005
  • DOI: 10.1093/pcp/pcr134
  • NAID: 10030305636
  • Peer Reviewed
• [Presentation] トマト日持ち性分子育種へのマイクロトムTILINGプラットフォームの利用可能性 (2012)
  • Author(s): 岡部佳弘, 浅水恵理香, 有泉亨, 白澤健太, 田畑哲之, 江面浩
  • Organizer: 日本育種学会第120回講演会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2012-03-29
• [Presentation] Tomato ethylene receptor mutants sletr1-1 and sletr1-2 exhibit different levels of ethylene insensitivity and impaired fruit ripening phenotype (2012)
  • Author(s): Okabe Y, Asamizu E, Ariizumi T, Ezura H.
  • Organizer: The IXth International Conference on the Plant Hormone Ethylene
  • Place of Presentation: Rotorua Convention Centre, Rotorua, New Zealand
  • Year and Date: 2012-03-20
• [Presentation] Tomato ethylene receptor mutants sletrl-1 and sletrl-2 exhibit different levels of ethylene insensitivity and impaired fruit ripening phenotype (2012)
  • Author(s): Yoshihiro Okabe, Erika Asamizu, Tohru Ariizumi, Hiroshi Ezura
  • Organizer: The IXth International Conference on the Plant Hormone Ethylene
  • Place of Presentation: Rotorua Convention Centre, Rotorua, New Zealand
  • Year and Date: 2012-03-20
• [Presentation] Micro-Tom TILLING technology : A reverse genetic tool for the elucidation of important traits in tomato (2012)
  • Author(s): Ezura H, Okabe Y.
  • Organizer: 第53回日本植物生理学会年会(招待講演)
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-16
• [Presentation] Micro-Tom TILLING technology : A reverse genetic tool for the elucidation of important traits in tomato (2012)
  • Author(s): Hiroshi Ezura, Yoshihiro Okabe
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(招待講演)
  • Year and Date: 2012-03-16
• [Presentation] Isolation and characterization of Sletr1 mutant alleles exhibiting different levels of ethylene insensitivity and impaired fruit ripening phenotype (2011)
  • Author(s): Okabe Y, Asamizu E, Ariizumi T, Ezura H.
  • Organizer: 8th Solanaceae and 2nd Cucurbitaceae Genome Conference
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2011-11-28
• [Presentation] Isolation and characterization of Sletrl mutant alleles exhibiting different levels of ethylene insensitivity and impaired fruit ripening phenotype (2011)
  • Author(s): Yoshihiro Okabe, Erika Asamizu, Tohru Ariizumi, Hiroshi Ezura
  • Organizer: 8^<th> Solanaceae and 2^<nd> Cucurbitaceae Genome Conference
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2011-11-28
• [Presentation] トマト重要形質解析のためのTILLING技術の基盤開発 (2011)
  • Author(s): 岡部佳弘, 浅水恵理香, 斎藤岳士, 松倉千昭, 有泉亨, 溝口剛, 江面浩
  • Organizer: 日本育種学会第120回講演会
  • Place of Presentation: 福井県立大学
  • Year and Date: 2011-09-24
• [Presentation] Development of Micro-Tom TILLING platform (2011)
  • Author(s): Okabe Y, Asamizu E, Saito T, Ariizumi T, Mizoguchi T, Matsukura C, Ezura H.
  • Organizer: 日本ナス科・ウリ科合同国際シンポジウム
  • Place of Presentation: 岡山大
• [Presentation] Development of Micro-Tom TILL-NG platform. (2011)
  • Author(s): Okabe Y, Asamizu E, Saito T, Ariizumi T, Mizoguchi T, Matsukura C, Ezura H
  • Organizer: 日本ナス科・ウリ科合同国際シンポジウム
  • Place of Presentation: 岡山大学
• [Presentation] Development of Micro-Tom TILLING platform (2010)
  • Author(s): Okabe Y, Asamizu E, Saito T, Ariizumi T, Mizoguchi T, Matsukura C, Ezura H.
  • Organizer: The 7th Solanaceae Conference, SOL2010
  • Place of Presentation: Dundee, Scotland
• [Presentation] Development of Micro-Tom TILL-NG platform. (2010)
  • Author(s): Okabe Y, Asamizu E, Saito T, Ariizumi T, Mizoguchi T, Matsukura C, Ezura H
  • Organizer: The 7^<th> Solanaceae Conference, SOL2010
  • Place of Presentation: Dundee, Scotland
• [Remarks]
  • URL: http://www.gene.tsukuba.ac.jp/Plant/MolecularBreeding/index.html