Identification of polyembryogenesis regulation gene in Citrus and diversification.

• Project/Area Number: 21580005
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Shizuoka University
• Principal Investigator: OMURA Mitsuo 静岡大学, 農学部, 教授 (90355397)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 遺伝子 / ゲノム / 育種 / 発生・分化 / 育種学

Research Abstract

To understand the molecular mechanism of apomixis in plant, the positional cloning method was applied to identify the gene regulating nucellar embryogenesis in Citrus. The 380 kb covering the locus responsible to somatic embryogenesis on the linkage group 1 was sequenced and the diversification of the region was surveyed. The ORFs detected in the region were compared in their structure and expression between polyembryony and monoembryony haplotypes. The genes expressing during the somatic embryogenesis from cultured callus were also explored to associate with the responsible gene in nucellar embryogenesis.

Research Products

• [Journal Article] Characterization of genomic sequence showing strong association wit hpolyembryony among diverse Citrus species and cultivars, and its synteny with Vitis and Populus (2012)
  • Author(s): Nakano, M., T. Shimada, T. Endo, H. Fujii, H. Nesumi, M. Kita, M. Ebina, T. Shimizu and M. Omura
  • Journal Title: Plant Science Volume: 183 Pages: 131-142
  • Peer Reviewed
• [Journal Article] Characterization of genomic sequence showing strong association with polyembryony among diverse Citrus species and cultivars, and its synteny with Vitis and Populus (2012)
  • Author(s): Nakano, M., T.Shimada, T.Endo, H.Fujii, H.Nesumi, M.Kita, M.Ebina, T.Shimizu, M.Omura
  • Journal Title: Plant Science Volume: 183 Pages: 131-142
  • Peer Reviewed
• [Journal Article] Quantitative trait loci(QTL) analysis of carotenoid content in Citrus fruit (2011)
  • Author(s): Sugiyama, A., M. Omura, H. Matsumoto, T. Shimada, H. Fujii, T. Endo, T. Shimizu, H. Nesumi and Y. Ikoma
  • Journal Title: J. Japan. Soc. Hort. Sci Volume: 80 Pages: 136-144
  • NAID: 40018755460
  • Peer Reviewed
• [Journal Article] PCR primer for marker assisted backcrossing to introduce a CTV resistance gene from Poncirus trifoliate(L.) Raf. into Citurs. (2011)
  • Author(s): Ohta, S., T. Endo, T. Shimada, H. Fujii, T. Shimizu, T. Kuniga, T. Yoshioka, H. Nesumi, T. Yoshida, and M. Omura
  • Journal Title: J. Japan. Soc. Hort. Sci Volume: 80 Pages: 295-307
  • Peer Reviewed
• [Journal Article] Quantitative trait loci (QTL) analysis of carotenoid content in Citrus fruit (2011)
  • Author(s): Sugiyama, A., M.Omura, H.Matsumoto, T.Shimada, H.Fujii, T.Endo, T.Shimizu, H.Nesumi, Y.Ikoma
  • Journal Title: J.Japan.Soc.Hort.Sci. Volume: 80 Pages: 136-144
  • NAID: 40018755460
  • Peer Reviewed
• [Journal Article] PCR primer for marker assisted backcrossing to introduce a CTV resistance gene from Poncirus trifoliate (L.) Raf.into Citurs (2011)
  • Author(s): Ohta, S., T.Endo, T.Shimada, H.Fujii, T.Shimizu, T.Kuniga, T.Yoshioka, H.Nesumi, T.Yoshida, M.Omura
  • Journal Title: J.Japan.Soc.Hort.Sci. Volume: 80 Pages: 295-307
  • Peer Reviewed
• [Journal Article] Structure and expression levels of alleles of Citrus zeaxanthine epoxidase genes (2010)
  • Author(s): Sugiyama, A., Y. Ikoma, H. Fujii, T. Shimada, T. Endo, T. Shimizu and M. Omura
  • Journal Title: J. Japan. Soc. Hort. Sci Volume: 79 Pages: 263-274
  • NAID: 130004726128
  • Peer Reviewed
• [Journal Article] Structure and expression levels of alleles of Citrus zeaxanthine epoxidase genes. (2010)
  • Author(s): Sugiyama, A., Y.Ikoma, H.Fujii, T.Shimada, T.Endo, T.Shimizu, M.Omura
  • Journal Title: J. Japan. Soc. Hort. Sci. Volume: 79 Pages: 263-274
  • NAID: 130004726128
  • Peer Reviewed
• [Presentation] カンキツ連鎖地図STSマーカーのクレメンチンゲノム配列との対応づけ (2012)
  • Author(s): 大村三男・中野道治・藤井浩・遠藤朋子・島田武彦・清水徳朗・喜多正幸・吉岡照高
  • Organizer: 園芸学会平成24年度春季大会
  • Place of Presentation: 大阪府立大学(大阪府)
  • Year and Date: 2012-03-29
• [Presentation] カンキツ多胚性遺伝子座領域における予測ORFの発現解析 (2011)
  • Author(s): 小澤綾・中野道治・遠藤朋子・藤井浩・島田武彦・清水徳朗・喜多正幸・吉岡照高・大村三男
  • Organizer: 園芸学会平成23年度秋季大会
  • Place of Presentation: 岡山大学(岡山県)
  • Year and Date: 2011-09-26
• [Presentation] カンキツ多胚性遺伝子座領域における挿入欠失変異カンキツ多胚性遺伝子座領域における挿入欠失変異 (2011)
  • Author(s): 大村三男・中野道治・藤井浩・遠藤朋子・島田武彦・清水徳朗・喜多正幸・吉岡照高
  • Organizer: 園芸学会平成23年度秋季大会
  • Place of Presentation: 岡山大学(岡山県)
  • Year and Date: 2011-09-26
• [Presentation] カンキツ多胚性遺伝子座領域における挿入欠失変異 (2011)
  • Author(s): 大村三男・中野道治・藤井浩・遠藤朋子・島田武彦・清水徳朗・喜多正幸・吉岡照高
  • Organizer: 園芸学会平成23年度秋季大会
  • Place of Presentation: 岡山大学(岡山県)
  • Year and Date: 2011-09-26
• [Presentation] カンキツ多胚性遺伝子座領域におけるゲノム配列のハプロタイプ比較 (2011)
  • Author(s): 中野道治・小澤綾・遠藤朋子・藤井浩・島田武彦・清水徳朗・蝦名真澄・大村三男
  • Organizer: 園芸学会平成23年度春季大会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2011-03-21
• [Presentation] カンキツの不定胚形成過程におけるAP2様遺伝子の発現プロフィールの解析 (2010)
  • Author(s): 木越景子・中野道治・遠藤朋子・島田武彦・藤井浩・清水徳朗・大村三男
  • Organizer: 園芸学会平成22年度秋季大会
  • Place of Presentation: 大分大学
  • Year and Date: 2010-09-20
• [Presentation] マイクロアレイを用いたカンキツカルスからの胚形成過程における遺伝子発現プロフィールの解析 (2010)
  • Author(s): 木越景子・中野道治・遠藤朋子・島田武彦・藤井浩・清水徳朗・大村三男
  • Organizer: 園芸学会
  • Place of Presentation: 園芸学会
  • Year and Date: 2010-03-21
• [Presentation] Characterization of genomic sequence for the polyembryony locus in Citrus by expression and association analysis. (2010)
  • Author(s): Omura, M., M. Nakano, H. Fujii, K. Kigoshi, T. Shimada, T. Endo, T. Shimizu and M. Ebina
  • Organizer: The International Conference on the status of Plant & Animal genome research.(PAG-XVIII)
  • Place of Presentation: San Diego, CA, USA
• [Presentation] Characterization of genomic sequence for the polyembryony locus in Citrus by expression and association analysis (2010)
  • Author(s): Omura, M., M.Nakano, H.Fujii, K.Kigoshi, T.Shimada, T.Endo, T.Shimizu, M.Ebina.
  • Organizer: The International Conference on the status of Plant & Animal genome research. (PAG-XVIII)
  • Place of Presentation: San Diego, CA, USA
• [Presentation] マイクロアレイを用いたカンキツカルスからの胚形成過程における遺伝子発現プロフィールの解析 (2010)
  • Author(s): 木越景子・中野道治・遠藤朋子・島田武彦・藤井浩・清水徳朗・大村三男
  • Organizer: 園芸学会
  • Place of Presentation: 藤沢市日本大学
• [Presentation] カンキツ多胚性遺伝子座ゲノム領域の塩基配列決定と発現解析 (2009)
  • Author(s): 中野道治・藤井浩・木越景子・島田武彦・遠藤朋子・清水徳朗・蝦名真澄・大村三男
  • Organizer: 園芸学会
  • Place of Presentation: 秋田市秋田大学
  • Year and Date: 2009-09-26
• [Presentation] カンキツカルスからの胚形成過程における遺伝子発現プロフィールの解析 (2009)
  • Author(s): 木越景子・中野道治・遠藤朋子・島田武彦・藤井浩・清水徳朗・大村三男
  • Organizer: 園芸学会
  • Place of Presentation: 秋田市秋田大学
  • Year and Date: 2009-09-26
• [Book] 果物学-果物のなる樹のツリーウオッチング (2010)
  • Author(s): 八田洋章・大村三男
  • Total Pages: 388
  • Publisher: 東海大学出版会