Regulation of starch synthesis and tuberous root formation by a transcription factor WRKY in sweet potato

• Project/Area Number: 21580015
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Crop science/Weed science
• Research Institution: Kyushu University
• Principal Investigator: SAITOU Kazuyuki 九州大学, 大学院・農学研究院, 准教授 (00215534)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 食用作物 / サツマイモ / 転写因子 / WRKY / 転写制御 / ADP-グルコースピロホスホリラーゼ / サイクリンD3 / シロイヌナズナ / AtWRKY20

Research Abstract

ADP-glucose pyrophosporylase(AGPase) catalyzes the first limiting step in starch biosynthesis. In this study, a WRKY transcription factor cDNA, AtWRKY20, has been isolated from Arabidopsis thaliana and AtWRKY20 was a direct transcriptional activator of the AGPase gene. AtWRKY20 interacted with the promoter of sweet potato AGPase gene and the expression of AGPase gene was enhanced by introduction of AtWRKY20 into sweet potato.

Research Products

• [Journal Article] Takashi Yuasa, Osamu Ueno, Activation of ADP-glucose pyrophosphorylase gene promoters by a WRKY transcription factor, AtWRKY20, in Arabidopsis thaliana L. and sweet potato(Ipomoea batatas Lam.) (2012)
  • Author(s): Takafumi Nagata, Hiromich Hara, Kazuyuki Saitou, Akiko Kobashi, Kengo Kojima
  • Journal Title: Plant Production Science Volume: Vol.15, No.1 Pages: 10-18
  • Peer Reviewed
• [Journal Article] Activation of ADP-Glucose Pyrophosphorylase Gene Promoters by a WRKY Transcription Factor, AtWRKY20, in Arabidopsis thaliana L. and Sweet Potato (Ipomoea batatas Lam.) (2012)
  • Author(s): Nagata, et al
  • Journal Title: Plant Production Science Volume: 15 Issue: 1 Pages: 10-18
  • DOI: 10.1626/pps.15.10
  • NAID: 130004462250
  • ISSN: 1343-943X, 1349-1008
  • Peer Reviewed
• [Journal Article] Effects of nitrogen on the expression of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit multigene family members in rice (2012)
  • Author(s): Miyazaki, et al
  • Journal Title: Plant Production Science Volume: 15(In press)
  • Peer Reviewed
• [Journal Article] Regulation of expression of D3-type cyclins and ADP-glucose pyrophosphorylase genes by sugar, cytokinin and ABA in sweet potato(Ipomoea batatas Lam.) (2009)
  • Author(s): Takafumi Nagata, Kazuyuki Saitou
  • Journal Title: Plant Production Science Volume: Vol.12, No.4 Pages: 434-442
  • NAID: 130004462188
  • Peer Reviewed
• [Journal Article] 、シロイヌナズナの転写因子AtWRKY20はADP-グルコースピロホスホリラーゼ大サブユニット遺伝子ApL3の転写活性を高める (2009)
  • Author(s): 原大道、永田敬文、児嶌健吾、湯淺高志、齋藤和幸、上野修
  • Journal Title: 日本作物学会紀事 Volume: 第78巻、別号2 Pages: 136-137
  • NAID: 10030133478
• [Journal Article] サツマイモにおけるADP-グルコースピロホスホリラーゼ小サブユニット遺伝子ibAGP1の転写活性はシロイヌナズナAtWRKY20転写因子により高まる (2009)
  • Author(s): 永田敬文、原大道、齋藤和幸、上野修
  • Journal Title: 日本作物学会紀事 Volume: 第78巻、別号2 Pages: 138-139
  • NAID: 10030133479
• [Journal Article] Regulation of expression of D3-type cyclins and ADP-glucose pyro-phosphorylase genes by sugar, cytokinin and ABA in sweet potato(Ipomoea batatas Lam.) (2009)
  • Author(s): Nagata, et al
  • Journal Title: Plant Production Science 12 Pages: 434-442
  • Peer Reviewed
• [Journal Article] シロイヌナズナの転写因子AtWRKY20はADP-グルコースピロホスホリラーゼ大サブユニット遺伝子ApL3の転写活性を高める (2009)
  • Author(s): 原大道, ら
  • Journal Title: 日本作物学会紀事 78別号2 Pages: 136-137
• [Journal Article] サツマイモにおけるADP-グルコースピロホスホリラーゼ小サブユニット遺伝子ibAGP1の転写活性はシロイヌナズナAtWRKY20転写因子により高まる (2009)
  • Author(s): 永田敬文, ら
  • Journal Title: 日本作物学会紀事 78別号2 Pages: 137-138
• [Journal Article] Activation of ADP-glucose pyrophosporylase gene promoters by a WRKY transcription factor, AtWRKY20, in Arabidopsis thaliana L.and sweet potato (Ipomoea batatas Lam.)
  • Author(s): Nagata, et al
  • Journal Title: Plant Production Science Volume: (掲載決定)
  • Peer Reviewed
• [Presentation] イネの窒素によるOsDREB1GとOsbZIP62の相互作用を介したRubiscoスモールサブユニット遺伝子、OsRBCS3、の転写制御 (2011)
  • Author(s): 齋藤和幸
  • Organizer: 日本作物学会第232回講演会
  • Place of Presentation: 山口大学共通教育棟(山口)
  • Year and Date: 2011-09-02
• [Presentation] OsDREB1E転写因子によるイネRubiscoスモールサブユニット遺伝子、OsRBCS3、の窒素に応答した転写制御 (2011)
  • Author(s): 齋藤和幸
  • Organizer: 日本作物学会第232回講演会
  • Place of Presentation: 山口大学共通教育棟(山口)
  • Year and Date: 2011-09-02
• [Presentation] OsHAP5CによるイネRubiscoスモールサブユニット遺伝子の窒素に応答した転写制御 (2010)
  • Author(s): 永倉大輝, 他
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-08
• [Presentation] The rice OsHAP5C gene encoding a subunit of the CCAAT box bindingcomplex regulates the expression of Rubisco small subunit gene in response to nitrogen supply (2010)
  • Author(s): Miyazaki, et al
  • Organizer: The 15^<th> International Congress of Photosynthesis
  • Place of Presentation: Friendship Hotel (Beijing, China)
• [Presentation] The rice OsDREB1G transcription factor regulates gene expression of Rubisco small subunit in response to nitrogen supply (2010)
  • Author(s): Miyazaki, et al
  • Organizer: 3^<rd> International Rice Congress
  • Place of Presentation: National Convention Center (Hanoi, Vietnam)
• [Presentation] シロイヌナズナの転写因子AtWRKY20はADP-グルコースピロホスホリラーゼ大サブユニット遺伝子ApL3の転写活性を高める (2009)
  • Author(s): 原大道ら
  • Organizer: 日本作物学会
  • Place of Presentation: 静岡県コンベンションアーツセンター(グランシップ)
  • Year and Date: 2009-09-30
• [Presentation] サツマイモにおけるADP-グルコースピロホスホリラーゼ小サブユニット遺伝子ibAGP1の転写活性はシロイヌナズナAtWRKY20転写因子により高まる (2009)
  • Author(s): 永田敬文ら
  • Organizer: 日本作物学会
  • Place of Presentation: 静岡県コンベンションアーツセンター(グランシップ)
  • Year and Date: 2009-09-30
• [Remarks]
  • URL: http://www.agr.kyushu-u.ac.jp/lab/shokusei/
• [Remarks]
  • URL: http://bbs1.agr.kyushu-u.ac.jp/prweb2/b07/