The gene required for the plant pathogenic and mycotoxin producing fungus to spread in the host

• Project/Area Number: 21580055
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Gifu University
• Principal Investigator: SUGA Haruhisa 岐阜大学, 生命科学総合研究支援センター, 准教授 (20283319)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 菌類 / 遺伝子 / マイコトキシン / ムギ類赤かび病 / Fusarium

Research Abstract

Fusarium head blight fungus is a plant pathogen that causes mycotoxin contamination in major cereals. A natural pathogenicity mutant that could not spread beyond the inoculation point in host was found. The mutation locus was mapped to 54Kb-span in the chromosome 2by linkage analyses and3Kb in this region was deleted in the mutant. The FGSG0 2810 gene within the 3Kb of wild type strain was cloned and introduced into the mutant by transformation. Introduction of the FGSG02810 gene restored pathogenicity of the mutant. These results indicate that FGSG02810 is an essential gene for Fusarium head blight fungus to spread in the host plant.

Research Products

• [Journal Article] The genetic profile andmolecular diagnosis of thiophanate-methyl resistant strainsof Fusarium asiaticum in Japan (2011)
  • Author(s): Suga H, Nakajima T, Kageyama K, Hyakumachi M
  • Journal Title: FungalBiology Volume: 115 Pages: 1244-1250
  • Peer Reviewed
• [Journal Article] Amultiplex PCR for the detection of Phytophthora nicotianae and P. cactorum, and a survey of their occurrence in strawberry production areas of Japan (2011)
  • Author(s): Li M, Asano T, Suga H, Kageyama K
  • Journal Title: Plant Disease Volume: 95 Pages: 1270-1278
  • Peer Reviewed
• [Journal Article] Rice dwarf viruses with dysfunctional genomes generated in plants are filtered out in vectorinsects : implications for the origin ofthe virus (2011)
  • Author(s): Pu Y, Kikuchi A, Moriyasu Y, Tomaru M, Jin Y, Suga H, Hagiwara K, Akita F, Shimizu T, Netsu O, Suzuki N, Uehara-Ichiki T, Sasaya T, Wei T, Li Yi, Omura T
  • Journal Title: Journal of Virology Volume: 85 Pages: 2975-2979
  • Peer Reviewed
• [Journal Article] The genetic profile and molecular diagnosis of thiophanate-methyl resistant strains of Fusarium asiaticum in Japan (2011)
  • Author(s): Suga H, Nakajima T, Kageyama K, Hyakumachi M
  • Journal Title: Fungal Biology Volume: 115 Issue: 12 Pages: 1244-1250
  • DOI: 10.1016/j.funbio.2011.08.009
  • Peer Reviewed
• [Journal Article] Rice dwarf viruses with dysfunctional genomes generated in plants are filtered out in vector insects : implications for the origin of the virus (2011)
  • Author(s): Pu Y, Kikuchi A, Moriyasu Y, Tomaru M, Jin Y, Suga H, Hagiwara K, Akita F, Shimizu T, Netsu O, Suzuki N, Uehara-Ichiki T, Sasaya T, Wei T, Li Yi, Omura T
  • Journal Title: Journal of Virology Volume: 85 Issue: 6 Pages: 2975-2979
  • DOI: 10.1128/jvi.02147-10
  • Peer Reviewed
• [Journal Article] A multiplex PCR for the detection of Phytophthora nicotianae and P.cactorum, and a survey of their occurrence in strawberry production areas of Japan (2011)
  • Author(s): Li M, Asano T, Suga H, Kageyama K
  • Journal Title: Plant Disease Volume: 95 Issue: 10 Pages: 1270-1278
  • DOI: 10.1094/pdis-01-11-0076
  • Peer Reviewed
• [Journal Article] Development of multiplex PCR to detectfive Pythium species related to turfgrass diseases (2010)
  • Author(s): Asano T, Senda M, Suga H, Kageyama K
  • Journal Title: Journal of Phytopathology Volume: 158 Pages: 609-615
  • Peer Reviewed
• [Journal Article] Development of realtime PCR technique for the estimation of population density of Pythium intermedium in forest soils (2010)
  • Author(s): Li M, Senda M, Komatsu T, Suga H, Kageyama K
  • Journal Title: Microbiological Research Volume: 165 Pages: 695-705
  • Peer Reviewed
• [Journal Article] Development of multiplex PCR to detect five Pythium species related to turfgrass diseases. (2010)
  • Author(s): Asano T, Senda M, Suga H, Kageyama K
  • Journal Title: Journal of Phytopathology Volume: 158 Pages: 609-615
  • Peer Reviewed
• [Journal Article] Development of mrealtime PCR technique for the estimation of population density of Pythium intermedium in forest soils. (2010)
  • Author(s): Li M, Senda M, Komatsu T, Suga H, Kageyama K
  • Journal Title: Microbiological Research Volume: 165 Pages: 695-705
  • Peer Reviewed
• [Journal Article] 静岡県西部地域のススキから分離されたFusarium graminearum (2009)
  • Author(s): 外側正之, 須賀晴久
  • Journal Title: 関西病虫研報 Volume: 51 Pages: 55-56
  • NAID: 130004773692
  • Peer Reviewed
• [Journal Article] Populationstructure of the Fusarium graminearum species complex from a single Japanese wheat field sampled in two consecutive years (2009)
  • Author(s): Karugia GW, Suga H, Gale LR, Nakajima T, Tomimura K, Hyakumachi M
  • Journal Title: Plant Disease Volume: 93 Pages: 170-174
  • Peer Reviewed
• [Journal Article] Population structure of Fusarium asiaticum from two Japanese regions and eastern China (2009)
  • Author(s): Karugia GW, Suga H, Gale LR, Nakajima T, Ueda A, Hyakumachi M
  • Journal Title: Journal of General Plant Pathology Volume: 75 Pages: 110-118
  • NAID: 10029717564
  • Peer Reviewed
• [Journal Article] 静岡県西部地域のススキから分離されたFusarium graminearum (2009)
  • Author(s): 外側正之、須賀晴久
  • Journal Title: 関西病虫研報 51巻 Pages: 55-56
  • NAID: 130004773692
  • Peer Reviewed
• [Journal Article] ムギ類赤かび病菌の病原性と子のう殻形成能の両方に関与するゲノム領域の構造 (2009)
  • Author(s): 須賀晴久、中島隆、景山幸二、百町満朗
  • Journal Title: 日本植物病理学会報 75巻 Pages: 195-196
• [Journal Article] Population structure of the Fusarium graminearum species complex from a single Japanese wheat field sampled in two consecutive years (2009)
  • Author(s): Karugia GW, Suga H, Gale LR, Nakajima T, Tomimura K, Hyakumachi M
  • Journal Title: Plant Disease VOL.93 Pages: 170-174
  • Peer Reviewed
• [Journal Article] Population structure of Fusarium asiaticum from two Japanese regions and eastern China (2009)
  • Author(s): Karugia GW, Suga H, Gale LR, Nakajima T, Ueda A, Hyakumachi M
  • Journal Title: J Gen Plant Pathol VOL.75 Pages: 110-118
  • NAID: 10029717564
  • Peer Reviewed
• [Presentation] Fusarium fujikuroiの2系統分化を支持する一塩基多型の検出 (2012)
  • Author(s): 須賀晴久,月星隆雄,上垣隆一,中島隆,景山幸二,百町満朗
  • Organizer: 日本植物病理学会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2012-03-28
• [Presentation] Fusarium fujikuroiのフモニシン産生能の有無を識別するDNAマーカーの開発 (2012)
  • Author(s): 須賀晴久,月星隆雄,上垣隆一,中島隆,景山幸二,百町満朗
  • Organizer: 日本マイコトキシン学会
  • Place of Presentation: タワーホール船堀(東京都)
  • Year and Date: 2012-01-06
• [Presentation] Naturaldeletion of FGSG02810 gene lost both of pathogenicity and perithecium development in Fuasarium graminearum species complex (2011)
  • Author(s): Suga H, Scott T, Hirata Y, Nakajima T, Kageyama K, Hyakumachi M
  • Organizer: International Union of Microbiological Societies(IUMS) 2011Congress
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-06
• [Presentation] Natural deletion of FGSG02810 gene lost both of pathogenicity and perithecium development in Fuasarium graminearum species complex (2011)
  • Author(s): Suga H, Scott T, Hirata Y, Nakajima T, Kageyama K, Hyakumachi M
  • Organizer: International Union of Microbiological Societies (IUMS) 2011 Congress
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-06
• [Presentation] PCRによるイチゴ萎黄病菌の同定 (2011)
  • Author(s): 須賀晴久
  • Organizer: 第8回フザリウム研究
  • Place of Presentation: 福山市鞆公民館(広島県)
  • Year and Date: 2011-08-25
• [Presentation] PCRによるイチゴ萎黄病菌の同定 (2011)
  • Author(s): 須賀晴久
  • Organizer: 第8回フザリウム研究会
  • Place of Presentation: 福山市鞆公民館(広島県)
  • Year and Date: 2011-08-25
• [Presentation] FGSG02810遺伝子はムギ類赤かび病菌の病原性と子のう殻形成能の両方に関与する (2011)
  • Author(s): 須賀晴久,スコット暁子,平田有紀,中島隆,景山幸二,百町満朗
  • Organizer: 日本植物病理学会
  • Place of Presentation: 東京農工大学府中キャンパス(東京都)
  • Year and Date: 2011-03-27
• [Presentation] Fusarium fujikuroiのフモニシン産生クラスターの構造解明 (2011)
  • Author(s): 北嶋美葉,スコット暁子,久城真代,中島隆,景山幸二,百町満朗,須賀晴久
  • Organizer: 日本マイコトキシン学会
  • Place of Presentation: タワーホール船堀(東京都)
  • Year and Date: 2011-01-07
• [Presentation] Fusarium fujikuroiのフモニシン産生クラスターの構造解明 (2011)
  • Author(s): 北嶋美葉、スコット暁子、久城真代、中島隆、景山幸二、百町満朗、須賀晴久
  • Organizer: 日本マイコトキシン学会
  • Place of Presentation: ワターホール船堀(東京都)
  • Year and Date: 2011-01-07
• [Presentation] 日本産Fusarium fujikuroi内に検出された分子系統とフモニシン産生能の関係 (2010)
  • Author(s): 南雲陸,深澤恵海,北嶋美葉,月星隆雄,上垣隆一,中島隆,景山幸二,百町満朗,須賀晴久
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: AOSSA(福井県)
  • Year and Date: 2010-09-30
• [Presentation] 日本産Fusarium fujikuroi内に検出された分子系統とフモニシン産生能の関係 (2010)
  • Author(s): 南雲陸,深澤恵海,北嶋美葉,月星隆雄,上垣隆一,中島隆,景山幸二,百町満朗,須賀晴久
  • Organizer: 日本マイコトキシン学会
  • Place of Presentation: 文部科学省研究交流センター(茨城県)
  • Year and Date: 2010-09-10
• [Presentation] ムギ類赤かび病菌におけるチオファネートメチル耐性化と伝播機構の分子遺伝学的研究 (2010)
  • Author(s): 須賀晴久
  • Organizer: 第20回殺菌剤耐性菌研究会シンポジウム
  • Place of Presentation: 京都テルサ(京都県)
  • Year and Date: 2010-04-21
• [Presentation] PCR-RFLPによるGibberella fujikuroi種複合体の種同定とフモニシン産生能 (2009)
  • Author(s): 南雲陸,山本裕子,北嶋美葉,中島隆,景山幸二,百町満朗,須賀晴久
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: 神戸大学(兵庫県)
  • Year and Date: 2009-10-17
• [Presentation] PCR-RELPによるGibberella fujikuroi種複合体の種同定とフモニシン産生能 (2009)
  • Author(s): 南雲陸、山本裕子、北嶋美葉、中島隆、景山幸二、百町満朗、須賀晴久
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: 神戸大学(兵庫県)
  • Year and Date: 2009-10-17