| Project/Area Number |
21580056
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant pathology
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| Research Institution | Okayama University |
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Principal Investigator |
KONDO Hideki 岡山大学, 資源植物科学研究所, 助教 (40263628)
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|---|
| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Nobuhiro 岡山大学, 資源植物科学研究所, 教授 (70206514)
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| Research Collaborator |
MARUYAMA Kazuyuki 岡山大学, 資源植物科学研究所, 技術職員 (20379811)
IDA Bagus andika 中国浙江省農業科学院
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ランえそ斑紋ウイルス / ラブドウイルス / Nicotiana benthamiana / ウイルス工場 / バイロプラズム / 粒子形成 / 植物 / マイナス鎖RNAウイルス / 封入体 / 複製 / 核移行 / BiFC |
|---|
| Research Abstract |
Orchid fleck virus(OFV), a unique two-segmented negative-sense RNA virus, induces an intranuclear electron-lucent viroplasm(inclusion) in infected plant cells. We studied the molecular mechanism by which OFV viroplasms are produced in vivo. Transiently expression examinations by Agrobacterium-infiltration reveled that coexpression of nucleocapsid protein N and the putative phosphoprotein P, in the absence of virus infection, was sufficient for the formation of an intranuclear electron-lucent viroplasm-like structure in Nicotiana benthamiana cells.
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