Analysis of phospholipid turnovers in plant membrane systems that regulates infection of bacterial wilt pathogen, Ralstonia solanacearum

• Project/Area Number: 21580057
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Kochi University
• Principal Investigator: KIBA Akinori 高知大学, 教育研究部・総合科学系, 准教授 (50343314)
• Co-Investigator(Kenkyū-buntansha): HIKICHI Yasufumi 高知大学, 教育研究部・総合科学系, 教授 (70291507) ; OHNISHI Kouhei 高知大学, 教育研究部・総合科学系, 教授 (50211800)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: リン脂質 / フォスファチジン酸 / 植物免疫 / ジアシグリセロールキナーゼ / SEC14P / フォスファチジン酸フォスファターゼ / 情報伝達 / 青枯病菌 / ジアシルグリセロールキナーゼ / フォスフォリパーゼC / フォスフォリパーゼD / Nicotiana植物 / 防御応答

Research Abstract

Ralstonia solanacearum causes bacterial wilt in several economically important solanaceous. We isolated Sec14P and phosphodidic acid phosphtase(PAP), which are candidate genes related to plant immune response. From our results, Sec14P activate plant immune responses by the regulation of phospholipid turnover. In contrast, PAP negatively regulates plant defense responses by degradation of signaling phosphplipids.

Research Products

• [Journal Article] Lipid transfer protein homolog from gentian has an antimicrobial property and confirms resistance to Botrytis cinerea in transgenic tobacco (2012)
  • Author(s): Kiba A, Nakatsuka T, Yamamura S, Nishihara M
  • Journal Title: Plant Biotechnology Volume: in press
  • Peer Reviewed
• [Journal Article] Isolation and characterization of an asparagine-rich protein that regulates hypersensitive cell death-mediated resistance in Nicotiana plants (2012)
  • Author(s): Komori D, Nishihara M, Takahashi A, Gupta M, Yoshioka H, Mizumoto H, Ohnishi K, Hikichi Y, Kiba A
  • Journal Title: Plant Biotechnology Volume: in press
  • NAID: 10030804810
  • Peer Reviewed
• [Journal Article] 葯培養によるピーマン黒枯病抵抗性育種母本の育成 (2012)
  • Author(s): 下元祥史, 澤田博正, 岡田昌久, 森田泰彰, 水本祐之, 木場章範, 曵地康史
  • Journal Title: 日本植物病理学会誌 Volume: 78(In press)
  • NAID: 10030312036
  • Peer Reviewed
• [Journal Article] ナス黒枯病菌の再同定 (2011)
  • Author(s): 下元祥史, 森田泰彰, 竹内繁治, 曵地康史, 木場章範, 佐藤豊三
  • Journal Title: 日本植物病理学会誌 Volume: 77 Pages: 105-108
  • NAID: 130004497194
  • Peer Reviewed
• [Journal Article] Implication of an aldehyde dehydrogenase gene and a phosphinothricin N-acetyltransferase gene in diversity of Pseudomonas cichorii virulence (2011)
  • Author(s): Tanaka M, Wali UM, Nakayashiki H, Fukuda T, Mizumoto H, Ohnishi K, Kiba A, Hikichi Y
  • Journal Title: Gene Volume: 3 Pages: 62-80
  • Peer Reviewed
• [Journal Article] Implication of hrpW in virulence of Pseudomonas cichorii (2011)
  • Author(s): Kajihara S, Hojo, H, Koyanagi M, Tanaka M, Mizumoto H, Ohnishi K, Kiba A, Hikichi Y
  • Journal Title: Plant Pathology Volume: 61 Pages: 355-363
  • Peer Reviewed
• [Journal Article] S glycoprotein-like protein regulates defense responses in Nicotiana plants against Ralstonia solanacearum (2010)
  • Author(s): Maimbo M, Ohnishi K, Hikichi Y, Yoshioka H, Kiba A
  • Journal Title: Plant Physiology Volume: 152 Pages: 2023-2035
  • Peer Reviewed
• [Journal Article] Induction of reactive oxygen generation and functional changes in mitochondria and their involvement in the development of bacterial rot in lettuce caused by Pseudomonas cichorii (2009)
  • Author(s): Kiba A, Lee KY, Ohnishi K, Park P, Nakayashiki H, Tosa Y, Mayama S, Hikichi Y
  • Journal Title: Physiological Molecular Plant Pathology Volume: 74 Pages: 45-54
  • Peer Reviewed
• [Journal Article] Induction of the response regulator hrpG gene expression and activation of HrpG by phophorylation are controlled by distinct signal cascades in Ralstonia solanacearum (2009)
  • Author(s): Yoshimochi, T.
  • Journal Title: Journal of General Plant Pathology 75 Pages: 196-204
  • Peer Reviewed
• [Journal Article] Implication of C-terminal mutation of PopA of Ralstonia solanacearum strain OE1-1 in development of bacterial wilt (2009)
  • Author(s): Kanda, A.
  • Journal Title: Plant Pathology 58 Pages: 159-169
  • Peer Reviewed
• [Journal Article] Molecular typing of Japanese strains of Ralstonia solanacearum in relation to the ability to induce a hypersensitive reaction in tobacco (2009)
  • Author(s): Liu, Y.
  • Journal Title: Journal of General Plant Pathology 75 Pages: 369-380
  • Peer Reviewed
• [Journal Article] Distribution of avirulence genes avrA and popP1 in 22 Japanese phylotype I strains of Ralstonia solanacearum (2009)
  • Author(s): Liu, Y.
  • Journal Title: Journal of General Plant Pathology 75 Pages: 362-368
  • Peer Reviewed
• [Presentation] Regulatory mechanisms of plant immunity by phosphatidic acid phoshatase (2012)
  • Author(s): Masahito Nakano, Masahiro Nishihara, Kouhei Ohnishi, Yasufumi Hikichi, and Akinori Kiba
  • Organizer: 2nd Korea-Japan Joint Symposium. Fukuoka
  • Year and Date: 2012-03-27
• [Presentation] Regulatory mechanisms of plant immunity by phosphatidic acid phosphatase (2012)
  • Author(s): Nakano M, Nishihara M, Ohnishi K, Hikichi Y, Kiba A
  • Organizer: 2012 Japan-Korea Joint Symposium
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-03-26
• [Presentation] ホスファチジン酸は植物免疫応答を制御する (2012)
  • Author(s): 中野真人,西原昌宏,大西浩平,曵地康史,木場章範
  • Organizer: 日本植物病理学会平成24年度大会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 青枯病菌抵抗性におけるホスファチジン酸の関与 (2012)
  • Author(s): 中野真人,西原昌宏,大西浩平,曵地康史,木場章範
  • Organizer: 日本植物生理学会第53回年会
  • Place of Presentation: 京都産業大学
• [Presentation] 青枯病菌抵抗性におけるホスファチジン酸の関与 (2012)
  • Author(s): 中野真人, 西原昌宏, 大西浩平, 曵地康史, 木場章範
  • Organizer: 第53回日本植物生理学会
  • Place of Presentation: 京都産業大学
• [Presentation] ホスファチジン酸は植物免疫応答を制御する (2012)
  • Author(s): 中野真人, 西原昌宏, 大西浩平, 曵地康史, 木場章範
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場
• [Presentation] ウイルス誘導ジーンサイレンシング法を用いた耐病性植物の作出と解析 (2011)
  • Author(s): 中野真人,大西浩平,曵地康史,木場章範
  • Organizer: 第29回日本植物細胞分子生物学会大会,福岡市, 9月6-8日
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2011-03-26
• [Presentation] Sec14 phospholipids trasnfer protein regulates phospholipid turnover during plant immune responses (2011)
  • Author(s): Kiba A, Nakano M, Yoshioka H, Ohnishi K, Hikichi Y
  • Organizer: The 4th Asian Symposium on Plant Lipids
  • Place of Presentation: The University of Hong Kong
• [Presentation] Phosphatidic acid phosphatase acts as a molecular switch in the plant immune system (2011)
  • Author(s): Nakano M, Nishihara M, Ohnishi K, Hikichi Y, Kiba A
  • Organizer: The 4th Asian Symposium on Plant Lipids
  • Place of Presentation: The University of Hong Kong
• [Presentation] ホスファチジン酸ホスファターゼはタバコ植物における防御応答の制御に関与する (2011)
  • Author(s): 中野真人,西原昌宏,大西浩平,曵地康史,木場章範
  • Organizer: 植物微生物研究会第21回研究交流会
  • Place of Presentation: 岡山大学
• [Presentation] Sec14Pを介したリン脂質代謝制御と植物免疫応答 (2011)
  • Author(s): 木場章範,中野真人,大西浩平,吉岡博文,曳地康史
  • Organizer: 第24回植物脂質シンポジウム
  • Place of Presentation: 東京大学
• [Presentation] 病害抵抗性と感受性における植物リン脂質シグナルの役割～ホスファチジン酸脱リン酸化酵素について(2)～ (2011)
  • Author(s): 中野真人,西原昌宏,大西浩平,曵地康史,木場章範
  • Organizer: 第24回植物脂質シンポジウム
  • Place of Presentation: 東京大学
• [Presentation] ウイルス誘導ジーンサイレンシング法を用いた耐病性植物の作出と解析 (2011)
  • Author(s): 中野真人、大西浩平、曵地康史、木場章範
  • Organizer: 第29回日本植物細胞分子生物学会大会
  • Place of Presentation: 九州大学箱崎キャンパス
• [Presentation] ホスファチジン酸ホスファターゼはタバコ植物における防御応答の制御に関与する (2011)
  • Author(s): 中野真人、西原昌宏、大西浩平、曵地康史、木場章範
  • Organizer: 植物微生物研究会第21回研究交流会
  • Place of Presentation: 岡山大学津島キャンパス
• [Presentation] 病害抵抗性と感受性のスイッチングに関わるNbPAPの機能解析 (2011)
  • Author(s): 中野真人、大西浩平、曵地康史、木場章範
  • Organizer: 平成23年度日本植物病理学会大会
  • Place of Presentation: 東京農工大
• [Presentation] ホスファチジン酸脱リン酸化酵素は植物免疫を負に制御する (2011)
  • Author(s): 中野真人、大西浩平、曵地康史、木場章範
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学川内キャンパス
• [Presentation] 病害抵抗性と感受性における植物リン脂質シグナルの役割～ホスファチジン酸脱リン酸化酵素について(2) (2011)
  • Author(s): 中野真人、西原昌宏、大西浩平、曵地康史、木場章範
  • Organizer: 第24回植物脂質シンポジウム
  • Place of Presentation: 東京大学駒場キャンパス
• [Presentation] ホスファチジン酸分解酵素であるNbPAPは病害感受性誘導因子である (2010)
  • Author(s): 中野真人、大西浩平、曵地康史、木場章範
  • Organizer: 平成22年度日本植物病理学会大会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2010-04-19
• [Presentation] A sec14P phospholipids transfer protein regulates plant immunity in Nicotiana plants in response to phytopathogenic bacteria (2010)
  • Author(s): Akinori Kiba
  • Organizer: 1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: 宮崎市
• [Presentation] NbPAPはNicotiana植物の病害感受性遺伝子である (2010)
  • Author(s): 中野真人,大西浩平,曵地康史,木場章範
  • Organizer: 日本植物生理学会第51回年会
  • Place of Presentation: 熊本大学
• [Presentation] リン脂質代謝系はNicotiana植物の防御応答の制御に関与する (2010)
  • Author(s): 木場章範,大西浩平,吉岡博文,曵地康史
  • Organizer: 日本植物生理学会第51回年会
  • Place of Presentation: 熊本大学
• [Presentation] 病害抵抗性・感受性決定に関わる植物の感染応答機構 (2010)
  • Author(s): 木場章範
  • Organizer: 平成22年度植物感染生理談話会
  • Place of Presentation: 佐賀県 虹ノ松原ホテル
• [Presentation] A sec14P phospholipids transfer protein regulates plant immunity in Nicotiana plants in response to phytopathogenic bacteria. (2010)
  • Author(s): Akinori Kiba
  • Organizer: 1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: 宮崎県 青島パームビーチホテル
• [Presentation] ホスファチジン酸脱リン酸化酵素は活性酸素種とジャスモン酸情報伝達経路の抑制を介して感受性を誘導する (2010)
  • Author(s): 中野真人
  • Organizer: 平成22年度植物感染生理談話会
  • Place of Presentation: 佐賀県 虹ノ松原ホテル
• [Presentation] リン脂質代謝系は Nicotiana 植物の防御応答の制御に関与する (2010)
  • Author(s): 木場章範
  • Organizer: 第51回日本植物生理学会
  • Place of Presentation: 熊本大学
• [Presentation] NbPAP は Nicotiana 植物の病害感受性遺伝子である (2010)
  • Author(s): 中野真人
  • Organizer: 第51回日本植物生理学会
  • Place of Presentation: 熊本大学
• [Presentation] SGT1と HSP90 は Nicotiana benthamiana-青枯病菌の親和性相互作用において防御応答の抑制に関与する (2010)
  • Author(s): 伊藤慎
  • Organizer: 第51回日本植物生理学会
  • Place of Presentation: 熊本大学
• [Presentation] Induction of S glycoprotein-like protein and its participation in defense responses in Nicotiana plants against Ralstonia solanacearum (2009)
  • Author(s): 木場章範
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: Quebec, Canada. Centredes congres
• [Presentation] Expressionof hrpG and activation of response regulator HrpG are controlled by distinct signal cascades in Ralstonia solanacearum (2009)
  • Author(s): Ohnishi K.
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: Quebec, Canada. Centredes congres
• [Presentation] A new positive regulator PrhG for the hrp regulon in Ralstonia solanacearum (2009)
  • Author(s): Zhang Y.
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: Quebec, Canada. Centredes congres
• [Presentation] Regulation mechanism of hrp regulon with a new positive regulator PrhG in Ralstonia solanacearum (2009)
  • Author(s): Zhang Y.
  • Organizer: 日本細菌学会第3回細菌学・若手コロッセウム
  • Place of Presentation: 宮崎市、サンホテルフエニックス
• [Presentation] Phosphatidic acid phosphatase acts as a negative regulator of defense responses induced in pathogenic bacteria-infected tobacco plants (2009)
  • Author(s): 中野真人
  • Organizer: The 2009 KSPP Fall Meeting and the 1st Japan-Korea Joint Symposium
  • Place of Presentation: Jeju KAL Hotel, Jeju, Korea
• [Presentation] S glycoprotein-like protein participates in defense responses in Nicotiana plants against Ralstonia solanacearum (2009)
  • Author(s): 木場章範
  • Organizer: The 2009 KSPP Fall Meeting and the 1st Japan-Korea Joint Symposium
  • Place of Presentation: Jeju KAL Hotel, Jeju, Korea
• [Presentation] SEC14P activates defense responses in Nicotiana plants via regulation of phospholipid metabolisms (2009)
  • Author(s): 木場章範
  • Organizer: 3rd Asian Symposium on Plant Lipids
  • Place of Presentation: Yokohama Media and Communications Center
• [Presentation] Phosphatidic acid phosphatase regulates jasmonic acid and reactive oxygen species signaling in defence responses (2009)
  • Author(s): 中野真人
  • Organizer: 3rd Asian Symposium on Plant Lipids
  • Place of Presentation: Yokohama Media and Communications Center
• [Book] 病害抵抗性・感受性決定に関わる植物の感染応答機構 (2010)
  • Author(s): 木場章範
  • Total Pages: 99
  • Publisher: 日本植物病理学会