Analysis of ancient structure of the W chromosome of the silkworm, Bombyx mori

• Project/Area Number: 21580063
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: ABE Hiroaki 東京農工大学, 大学院・農学研究院, 助教 (80222660)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 分子昆虫生物学 / 性染色体 / カイコ / W染色体 / レトロトランスポゾン / ゲノム / 転移因子 / クワコ / 鱗翅目 / レトロポゾン / 性決定

Research Abstract

I analyzed the structure of the W chromosome of the silkworm, Bombyx mori. I found that the DNA sequences of the W chromosome comprised nested structures of many transposable elements. The history of the chromosome organization and evolution(degeneration) would be expected to be better preserved on the W chromosome than on the other chromosomes.

Research Products

• [Journal Article] Interspecies linkage analysis of mo, a Bombyx mori locus associated with mosaicism and gynandromorphism (2012)
  • Author(s): Fujii Tsuguru
  • Journal Title: Genetica Volume: 139 Issue: 10 Pages: 1323-1329
  • DOI: 10.1007/s10709-012-9634-0
  • Peer Reviewed
• [Journal Article] Molecular phylogeny, laboratory rearing, and karyotype of the bombycid moth,… (2012)
  • Author(s): Daimon, T., Yago, M., Hsu, YF., et al
  • Journal Title: Journal of Insect Science Volume: 12
  • Peer Reviewed
• [Journal Article] Sex-linked transcription factor involved in a shift of sex-pheromone preference in the silkmoth Bombyx mori (2011)
  • Author(s): Fujii Tsuguru
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 108 Issue: 44 Pages: 18038-18043
  • DOI: 10.1073/pnas.1107282108
  • Peer Reviewed
• [Journal Article] 油蚕とメンブレントラフィック (2011)
  • Author(s): 藤井告・阿部広明・嶋田透
  • Journal Title: 蚕糸・昆虫バイオテック Volume: 80巻 Pages: 93-102
  • NAID: 10030280572
• [Journal Article] The silkworm W chromosome is a source of female-enriched piRNAs (2011)
  • Author(s): Kawaoka Shinpei
  • Journal Title: RNA Volume: 17 Issue: 12 Pages: 2144-2151
  • DOI: 10.1261/rna.027565.111
  • Peer Reviewed
• [Journal Article] Identification and characterization of the fusion transcript, composed of the apterous homolog and a putative protein phosphatase gene, generated by 1. 5-Mb interstitial deletion in the vestigial(Vg) mutant of Bombyx mori (2011)
  • Author(s): Fujii, T., H. Abe, S. Katsuma and T. Shimada
  • Journal Title: Insect Biochemistry and Molecular Biology Volume: Vol.41(5) Issue: 5 Pages: 306-312
  • DOI: 10.1016/j.ibmb.2011.01.007
  • Peer Reviewed
• [Journal Article] 油蚕のメンブレントラフィック (2011)
  • Author(s): 藤井告・阿部広明・嶋田透
  • Journal Title: 蚕糸・昆虫バイオテック Volume: 80 Pages: 93-102
  • Peer Reviewed
• [Journal Article] Novel non-autonomous transposable elements on the W chromosome of the silkworm, Bombyx mori (2010)
  • Author(s): Abe, H., T. Fujii, T. Shimada and K. Mita
  • Journal Title: Journal of Genetics Volume: Vol.89(3) Pages: 375-387
  • Peer Reviewed
• [Journal Article] Molecular analysis of sex chromosome-linked mutants in the silkworm Bombyx mori (2010)
  • Author(s): Fujii, T., H. Abe and T. Shimada
  • Journal Title: Journal of Genetics Volume: Vol.89(3) Pages: 365-374
• [Journal Article] Recent transposition of yabusame, a novel piggyBack-like transposable element in the genome of the silkworm, Bombyx mori (2010)
  • Author(s): Daimon, T., M. Mitsuhiro, S. Katsuma, H. Abe, K. Mita and T. Shimada
  • Journal Title: Genome Volume: Vol.53(8) Pages: 585-593
  • Peer Reviewed
• [Journal Article] Non-susceptibility to Bombyx densovirus type 1, Nid-1 and nsd-1, affect distinctsteps of the viral infection pathway (2010)
  • Author(s): Kidokoro, K., K. Ito, D. O. Ogoyi, H. Abe, K. Mita and K. Kadono-Okuda
  • Journal Title: Journal of Invertebrate Pathology Volume: Vol.103 Pages: 79-81
  • Peer Reviewed
• [Journal Article] Identification and molecular characterization of a sex chromosome rearrangement causing a soft and pliable(spli) larval body phenotype in the silkworm, Bombyx mori (2010)
  • Author(s): Fujii, T., S. Kuwazaki, K. Yamamoto, H. Abe, A. Ohnuma, S. Katsuma, K. Mita and T. Shimada
  • Journal Title: Genome Volume: Vol.53 Pages: 45-54
  • Peer Reviewed
• [Journal Article] Identification and molecular characterization of a sex chromosome… (2010)
  • Author(s): Fujii, T., et al.
  • Journal Title: Genome Volume: 53 Pages: 45-54
  • Peer Reviewed
• [Journal Article] Non-susceptibility to Bombyx densovirus type 1, Nid-1 and nsd-1,… (2010)
  • Author(s): Kidokoro K., et al.
  • Journal Title: Journal of Invertebrate Pathology Volume: 103 Pages: 79-81
  • Peer Reviewed
• [Journal Article] Recent transposition of yabusame, a novel piggy Bac like transposable… (2010)
  • Author(s): Daimon, T., et al.
  • Journal Title: Genome Volume: 53 Pages: 585-593
  • Peer Reviewed
• [Journal Article] Novel non-autonomous transposable elements on the W chromosome… (2010)
  • Author(s): Abe H., et al.
  • Journal Title: Journal of Genetics Volume: 89 Pages: 375-387
  • Peer Reviewed
• [Journal Article] Identification and molecular characterization of a sex chromosome... (2010)
  • Author(s): T.Fujii, et al.
  • Journal Title: Genome 53 Pages: 45-54
  • Peer Reviewed
• [Journal Article] Non-susceptibility to Bombyx densovirus type 1, Nid-1 and nsd-1, affect... (2010)
  • Author(s): K.Kidokoro, et al.
  • Journal Title: Journal of Invertebrate Pathology 103 Pages: 79-81
  • Peer Reviewed
• [Journal Article] Recent transposition of yabusame, a novel piggyBao-like transposable... (2010)
  • Author(s): T.Daimon, et al.
  • Journal Title: Genome (In press)
  • Peer Reviewed
• [Journal Article] Molecular analysis of sex chromosome-linked mutants in the silkworm... (2010)
  • Author(s): T.Fujii, et al.
  • Journal Title: Journal of Genetics (In press)
  • Peer Reviewed
• [Journal Article] The Bombyx ovary-derived cell line endogenously expresses PIWI/PIWI-interacting complexes (2009)
  • Author(s): Kawaoka, S., N. Hayashi, Y. Suzuki, H. Abe, S. Sugano, Y. Tomari, T. Shimada and S. Katsuma
  • Journal Title: RNA Volume: Vol.15(7) Pages: 1258-1264
  • Peer Reviewed
• [Journal Article] A Bombyx mandarina mutant exhibiting translucent larval skin is controlled by the molybdenum cofactor sulfurase gene (2009)
  • Author(s): Fujii, T., M. Ozaki, T. Masamoto, S. Katsuma, H. Abe and T. Shimada
  • Journal Title: Genes and Genetic Systems Volume: Vol.84 Pages: 147-152
  • NAID: 10024791746
  • Peer Reviewed
• [Journal Article] A Bombyx mandarin mutant exhibiting translucent larval skin is... (2009)
  • Author(s): T.Fujii, et al.
  • Journal Title: Genes and Genetic Systems 84 Pages: 147-152
  • Peer Reviewed
• [Journal Article] The Bombyx ovary-derived cell line endogenously expresses PIWI/PIWI... (2009)
  • Author(s): S.Kawaoka, et al.
  • Journal Title: RNA 15 Pages: 1258-1264
  • Peer Reviewed
• [Journal Article] Molecular phylogeny, laboratory rearing and karyotype of the bombycid moth, Trilocha varians(Lepidoptera : Bombycidae : Bombycinae)
  • Author(s): Daimon, T., M. Yago, Y-F. Hsu, T. Fujii, Y. Nakajima, R. Kokusho, H. Abe, S. Katsuma and T. Shimada
  • Journal Title: Journal of Insect Science Volume: Vol.12
  • Peer Reviewed
• [Presentation] カイコのアルビノ(al)はテトラヒドロビオプテリン(BH4)生合成関連遺伝子に変異を持つ (2012)
  • Author(s): 藤井告・川本宗孝・阿部広明
  • Organizer: 第56回日本応用動物昆虫学会大会
  • Place of Presentation: 近畿大学農学部
  • Year and Date: 2012-03-28
• [Presentation] カイコのアルビノ(al)はテトラヒドロビオプテリン(BH4)生合成関連遺伝子 (2012)
  • Author(s): 藤井告・川本宗孝・阿部広明
  • Organizer: 第56回日本応用動物昆虫学会大会
  • Place of Presentation: 近畿大学農学部・奈良
  • Year and Date: 2012-03-28
• [Presentation] カイコの変異体を用いた嗅覚・味覚の分子遺伝学的研究 (2012)
  • Author(s): 嶋田透・藤井告・藤井毅, ら
  • Organizer: 第56回日本応用動物昆虫学会大会シンポジウム
  • Place of Presentation: 奈良県新公会堂
  • Year and Date: 2012-03-27
• [Presentation] イチジクカサンのW染色体RAPDマーカーの探索 (2012)
  • Author(s): 惣中祐季・阿部広明・普後一
  • Organizer: 日本蚕糸学会第82回大会
  • Place of Presentation: 九州大学箱崎キャンパス
  • Year and Date: 2012-03-19
• [Presentation] 第11染色体を部分的に欠失したカイコの食性異常 (2012)
  • Author(s): 藤井告・久保真紀・阿部広明
  • Organizer: 日本蚕糸学会第82回大会
  • Place of Presentation: 九州大学箱崎キャンパス
  • Year and Date: 2012-03-19
• [Presentation] カイコのGe(giant egg)系統で同定した伴性遺伝子の構造異常 (2012)
  • Author(s): 藤井告・阿部広明・川本宗孝
  • Organizer: 日本蚕糸学会第82回大会
  • Place of Presentation: 九州大学箱崎キャンパス
  • Year and Date: 2012-03-18
• [Presentation] カイコのGe(giant egg)系統で同した伴性遺伝子の構造異常 (2012)
  • Author(s): 藤井告・阿部広明・川本宗孝, ら
  • Organizer: 日本蚕糸学会第82回大会
  • Place of Presentation: 九州大学箱崎キャンパス
  • Year and Date: 2012-03-18
• [Presentation] Genetic and genomic studies on the food habit of the silkworm (2012)
  • Author(s): Shimada, T., Daimon, T., et al
  • Organizer: IITG-DBT Biotech Hub Invited Lecture Series
  • Place of Presentation: Seminar Hall, Dep.Biote
  • Year and Date: 2012-02-28
• [Presentation] Genetic and genomic studies on the food habits of the silkworm (2012)
  • Author(s): Shimada, T., Daimon, T., et al
  • Organizer: Special Lecture for the Silver Jubilee of the…
  • Place of Presentation: CDFD Lecture Hall
  • Year and Date: 2012-02-24
• [Presentation] Genomic studies on the relationship between the silkworm and its… (2012)
  • Author(s): Shimada, T., Daimon, T., et al
  • Organizer: Genome Research : Current Challenges and…
  • Place of Presentation: KOKUYO HALL
  • Year and Date: 2012-01-21
• [Presentation] Genetic studies on the relationship between the silkworm, Bombyx… (2011)
  • Author(s): Shimada, T., Daimon, T., et al
  • Organizer: The 2^<nd> International Symposium on Bombyx…
  • Place of Presentation: 中国・西南大学
  • Year and Date: 2011-10-23
• [Presentation] p50T系統に生じた新規突然変異の遺伝学的解析 (2011)
  • Author(s): 藤井告・阿部広明・木内隆史
  • Organizer: 日本蚕糸学会第81回大会
  • Place of Presentation: 東京大学農学部キャンパス
  • Year and Date: 2011-03-21
• [Presentation] 桑園におけるクワコ(Bombyx mandarina)の産卵性について (2011)
  • Author(s): 横山岳・檜山はる・阿部広明
  • Organizer: 日本蚕糸学会第81回大会
  • Place of Presentation: 東京大学農学部キャンパス
  • Year and Date: 2011-03-21
• [Presentation] カイコとクワコのW染色体に共通して存在する転移因子の入れ子状態 (2011)
  • Author(s): 阿部広明・横山岳・高田守
  • Organizer: 日本蚕糸学会第81回大会
  • Place of Presentation: 東京大学農学部キャンパス
  • Year and Date: 2011-03-21
• [Presentation] 融合染色体の解離とZ染色体および第13染色体への再転座 (2011)
  • Author(s): 藤井告・桑崎誠剛・阿部広明
  • Organizer: 日本蚕糸学会第81回大会
  • Place of Presentation: 東京大学農学部キャンパス
  • Year and Date: 2011-03-21
• [Presentation] クワコの幼虫に寄生するハチ (2011)
  • Author(s): 阿部広明・横山岳・普後一
  • Organizer: 日本蚕糸学会81回大会
  • Place of Presentation: 東京大学農学部キャンパス
  • Year and Date: 2011-03-21
• [Presentation] カイコとクワコW染色体に共通して存在する転移因子の入れ子状態 (2010)
  • Author(s): 阿部広明・横山岳・高田守
  • Organizer: 日本蚕糸学会関東支部第61回大会
  • Place of Presentation: 蚕糸会館6階
  • Year and Date: 2010-11-18
• [Presentation] カイコ第11染色体の塩基配列上での火傷Buの原因領域の絞り込み (2010)
  • Author(s): 久保真紀・藤井告・阿部広明
  • Organizer: 日本蚕糸学会関東支部第61回大会
  • Place of Presentation: 蚕糸会館6階
  • Year and Date: 2010-11-18
• [Presentation] moはカイコの第14染色体に連鎖する (2010)
  • Author(s): 藤井告・阿部広明・山本公子
  • Organizer: 日本蚕糸学会関東支部第61回大会
  • Place of Presentation: 蚕糸会館6階
  • Year and Date: 2010-11-18
• [Presentation] moはカイコの第14染色体に連鎖する (2010)
  • Author(s): 藤井告・阿部広明・山本公子, ら
  • Organizer: 日本蚕糸学会関東支部第61回大会
  • Place of Presentation: 蚕糸会館6階(千代田区)
  • Year and Date: 2010-11-18
• [Presentation] カイコ第11染色体の塩基配列上での火傷Buの原因領域の絞り込み (2010)
  • Author(s): 久保真紀・藤井告・阿部広明, ら
  • Organizer: 日本蚕糸学会関東支部第61回大会
  • Place of Presentation: 蚕糸会館6階(千代田区)
  • Year and Date: 2010-11-18
• [Presentation] カイコとクワコW染色体に共通して存在する転移因子の入れ子状態 (2010)
  • Author(s): 阿部広明・横山岳・高田守, ら
  • Organizer: 日本蚕糸学会関東支部第61回大会
  • Place of Presentation: 蚕糸会館6階(千代田区)
  • Year and Date: 2010-11-18
• [Presentation] Bombyx Familyの進化 (2010)
  • Author(s): 味村正博・前川秀彰, ら
  • Organizer: 平成22年度日本蚕糸学会東北中部東海関西九州支部合同大会
  • Place of Presentation: てだこホール(沖縄県浦添市)
  • Year and Date: 2010-11-14
• [Presentation] Evolution of Bombyx family (2010)
  • Author(s): Ajimura M., Maekawa H., et al.
  • Organizer: 6^<th> INTERNATIONAL CONFERENCE ON WILD SILKMOTHS, 2010 Tokyo
  • Place of Presentation: 東京農業大学
  • Year and Date: 2010-09-23
• [Presentation] カイコの伴性突然変異spliとBtに認められる性フェロモン応答性の異常 (2010)
  • Author(s): 藤井告・藤井毅・阿部広明
  • Organizer: 公開シンポジウム「複合適応形質の進化」
  • Place of Presentation: 東京大学農学部2号館
  • Year and Date: 2010-09-17
• [Presentation] Silkworm gonadal transcriptome reveals piRNAs derived from the sex… (2010)
  • Author(s): Kawaoka S., Kadota K., et al.
  • Organizer: The 19^<th> CDB Meeting "RNA Sciences in Cell and Developmental Biology"
  • Place of Presentation: 理化学研究所発生・再生科学総合研究センター
  • Year and Date: 2010-05-11
• [Presentation] 青熟油(oa)の原因遺伝子はBmHPS5である (2010)
  • Author(s): 藤井告・伴野豊・阿部広明
  • Organizer: 日本蚕糸学会第80回記念大会
  • Place of Presentation: 信州大学繊維学部
  • Year and Date: 2010-04-04
• [Presentation] 青熟油(oa)の原因遣伝子はBmHPS5である (2010)
  • Author(s): 藤井告・伴野豊・阿部広明, ら
  • Organizer: 日本蚕糸学会第80回記念大会
  • Place of Presentation: 信州大学繊維学部
  • Year and Date: 2010-04-04
• [Presentation] カイコW染色体から産生されるpiRNA (2010)
  • Author(s): 河岡慎平・門田幸二・林伸光, ら
  • Organizer: 日本蚕糸学会第80回記念大会
  • Place of Presentation: 信州大学繊維学部
  • Year and Date: 2010-04-04
• [Presentation] カイコの伴性突然変異Vg(痕跡翅)に特異的なptpとapterousの融合遺伝子 (2010)
  • Author(s): 藤井告・阿部広明・勝間進
  • Organizer: 第54回日本応用動物昆虫学会大会
  • Place of Presentation: 千葉大学西千葉キャンパス
  • Year and Date: 2010-03-27
• [Presentation] カイコの伴性突然変異Vg(痕跡翅)に特異的なptpとapterousとの融合遺伝子 (2010)
  • Author(s): 藤井告・阿部広明・勝間進・嶋田透
  • Organizer: 第54回日本応用動物昆虫学会大会
  • Place of Presentation: 千葉大学
  • Year and Date: 2010-03-26
• [Presentation] W-chromosome-derived female-specific piRNAs and their possible… (2010)
  • Author(s): 河岡慎平・門田幸二・鈴木穣, ら
  • Organizer: BMB2010(第33回日本分子生物学会年会)
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] カイコの伴性突然変異spliとBtに認められる性フェロモン応答性の異常 (2009)
  • Author(s): 藤井告・藤井毅・阿部広明, ら
  • Organizer: 日本蚕糸学会関東支部第60回学術講演会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2009-11-06
• [Presentation] acj6 mutants of the silkworm Bombyx mori cannot discriminate mulberry... (2009)
  • Author(s): 藤井告, et al.
  • Organizer: The Eighth International Workshop on MOLECULAR
  • Place of Presentation: Creta, Greece
• [Book] Molecular Biology and Genetics of Lepidoptera(Contemporary Topics in Entomology) (2010)
  • Author(s): Abe, H., Fujii, T. and Shimada, T.
• [Book] Molecular Biology and Genetics of the Lepidoptera (2010)
  • Author(s): Abe H., Fujii T., Shimada T.
  • Total Pages: 362
  • Publisher: CRC Press
• [Book] Molecular Biology and Genetics of the Lepidoptera(Contemporary, Topics in...) (2010)
  • Author(s): Abe, H., Fujii, T.
  • Total Pages: 362
  • Publisher: CRC Press Taylor and Francis Group
• [Book] Short views on International Insect Molecular Biology (2009)
  • Author(s): Abe, H. and Fujii T.
  • Publisher: India