Development of novel drug screening process by using Drosophila disease models and silk worm Cypovirus function

• Project/Area Number: 21580067
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: YAMAGUCHI Masamitsu 京都工芸繊維大学, 工芸科学研究科, 教授 (00182460)
• Co-Investigator(Kenkyū-buntansha): MORI Hajime 京都工芸繊維大学, 工芸科学研究科, 教授 (80201812) ; HASHIMOTO Reina 京都工芸繊維大学, ベンチャーラボラトリー, 研究員 (20467492)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 昆虫 / 遺伝子 / 応用動物 / 生体分子 / 遺伝学

Research Abstract

β-sarcoglycan contains EGF-like amino acid sequences in its extracellular domain. In vitro analyses demonstrated thatβ-sarcoglycan can interacts with EGFR via this extracellular domain. Double mutant for Syntrophin1and2has been established. Analyses with this double mutant clarified in vivo roles of these two genes. Recombinantβ-sarcoglycan tagged with Cypovirus VP3has been successfully expressed in cultured Drosophila cells.

Research Products

• [Journal Article] Fibroblast growth factor-2(Fgf2) is an important factor that maintains cellular immaturity and contributes to aggressiveness of osteosarcoma (2012)
  • Author(s): Shimizu, T., Ishikawa, T., Iwai, S., Ueki, A., Sugihara, E., Onishi, N., Kuninaka, S., Miyamoto, T., Toyama, Y., Ijiri, H., Mori, H., Matsuzaki, Y., Yaguchi, T., Nishio, H., Kawakami, Y., Ikeda, Y., Saya, H.
  • Journal Title: Mol. Cancer Res Volume: 10 Pages: 454-468
  • Peer Reviewed
• [Journal Article] XNP/dATRX interacts with DREF in the chromatin to regulate gene expression (2012)
  • Author(s): Valadez-Graham, V., Yoshioka, Y., Velazquez, O., Kawamori, A. Vazquez, M., Neumann, A., Yamaguchi, M. and Zurita, M.
  • Journal Title: Nucleic Acids Res Volume: 40(4) Pages: 1460-1474
  • Peer Reviewed
• [Journal Article] The warts gene as a novel target of the Drosophila DRE/DREF transcription pathway (2012)
  • Author(s): Fujiwara, S., Ida, H., Yoshioka, Y., Yoshida, H. and Yamaguchi, M.
  • Journal Title: Am J. Cancer Res Volume: 2(1) Pages: 36-44
  • Peer Reviewed
• [Journal Article] Transcription factor NF-Y is involved in differentiation of R7 photoreceptor cell in Drosophila (2012)
  • Author(s): Yoshioka, Y., Ly, L. L. and Yamaguchi, M.
  • Journal Title: Biology Open Volume: 1 Issue: 1 Pages: 19-29
  • DOI: 10.1242/bio.2011013
  • Peer Reviewed
• [Journal Article] The warts gene as a novel target of the Drosophila DRE/DREF transcription pathway (2012)
  • Author(s): Fujiwara, S., Ida, H., Yoshioka, Y., Yoshida, H., Yamaguchi, M
  • Journal Title: Am J.Cancer Res. Volume: 2 Pages: 36-44
  • Peer Reviewed
• [Journal Article] XNP/dATRX interacts with DREF in the chromatin to regulate gene expression (2012)
  • Author(s): Valadez-Graham, V., Yoshioka, Y., Velazquez, O., Kawamori, A., Vazquez, M., Neumann, A., Yamaguchi, M., Zurita, M.
  • Journal Title: Nucleic Acids Res. Volume: 40 Pages: 1460-1474
  • Peer Reviewed
• [Journal Article] Effect of αB-crystallin on protein aggregation in Drosophila (2012)
  • Author(s): Tue, N.T., Shimaji, K., Tanaka, N., Yamaguchi, M.
  • Journal Title: J.Biomed.Biotech. Volume: 2012 Pages: 1-7
  • DOI: 10.1155/2012/252049
  • Peer Reviewed
• [Journal Article] Differential requirement for the N-terminal catalytic domain of the DNA polymerase ε p255 subunit in the mitotic cell cycle and the endocycle (2012)
  • Author(s): Suyari, O., Kawai, M., Ida, H., Yoshida, H., Sakaguchi, K., Yamaguchi, M.
  • Journal Title: Gene Volume: 495 Pages: 104-114
  • Peer Reviewed
• [Journal Article] Drosophila DREF acting via the JNK pathway is required for thorax development (2012)
  • Author(s): Yoshioka, Y., Tue, N.T., Fujiwara, S., Matsuda, R., Valadez-Gtaham, V., Zurita, M., Yamaguchi, M.
  • Journal Title: Genesis Volume: (印刷中) Issue: 8 Pages: 599-611
  • DOI: 10.1002/dvg.22017
  • Peer Reviewed
• [Journal Article] Roles of histone H3K9 methyltransferases during Drosophila spermatogenesis (2012)
  • Author(s): Ushijima Y., Inoue, Y.H., Konishi, T., Kitazawa, D., Yoshida, H., Shimaji, K., Kimura, H., Yamaguchi, M.
  • Journal Title: Chromosome Res. Volume: 20 Pages: 319-331
  • Peer Reviewed
• [Journal Article] Polycomb repressive complex 2-dependent and -independent functions of Jarid2 in transcriptional regulation in Drosophila (2012)
  • Author(s): Herz, H.-M., Mohan, M., Garrett, A.-S., Miller, C., Casto, D., Zhang, Y., Seidel, C., Haug, J.S., Florens, L., Washburn, M.P., Yamaguchi, M., Shiekhattar, R., Shilatifard, A.
  • Journal Title: Mol.Cell.Biol. Volume: 32 Pages: 319-331
  • Peer Reviewed
• [Journal Article] MLF is a conserved regulator of RUNX transcription factor activity involved in hematopoiesis (2012)
  • Author(s): Bras, S., Martin-Lanneree, S., Gobert, V., Auge, B., Breig, O., Sanial, M., Yamaguchi, M., Haenlin, M., Plessis, A., Waltzer, L
  • Journal Title: Proc.Natl.Acad.Sci.USA Volume: 109 Pages: 4986-4991
  • Peer Reviewed
• [Journal Article] Overexpression of ubiquitin carboxyl terminal hydrolase impairs multipie pathways during eye development in Drosophila melanogaster (2012)
  • Author(s): Thao, D.T.P, Thuy An, P.N., Yamaguchi, M., Thuoc, T.L
  • Journal Title: Cell and Tissue Research Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] The use of leukemia inhibitory factor immobilized on virus-derived polyhedra to support the proliferation of mouse embryonic and induced pluripotent stem cells (2011)
  • Author(s): Nishishita, N., Ijiri, H., Takenaka, C., Kobayashi, K., Goto, K., Kotani, E., Itoh, T., Mori, H., Kawamata, S.
  • Journal Title: Biomaterials Volume: 32 Pages: 3555-3563
  • Peer Reviewed
• [Journal Article] Characterization of a novel C-type lectin cDNA, CLEM20cDNA, specifically expressed in mouthparts of the flesh fly Sarcopahaga peregrina (2011)
  • Author(s): Yamamoto-Kihara, M., Kotani, E., Mori, H.
  • Journal Title: J. Insect Biotech. Sericol Volume: 80 Pages: 31-39
  • Peer Reviewed
• [Journal Article] Regulation of intestinal stem cell proliferation by human methyl-CpG-binding protein-2in Drosophila (2011)
  • Author(s): Lee, S.-H., Kim, I-J., Kim, J.-G., Kim, Y.-S., Yamaguchi, M. and Yoo, M.-A.
  • Journal Title: Cell Struct. Funct Volume: 36(2) Pages: 197-208
  • Peer Reviewed
• [Journal Article] Essential role of Duox in the stabilization of Drosophila wing (2011)
  • Author(s): Tu Anh, N. T., Nishitani, M., Harada, S., Yamaguchi, M. and Kamei, K.
  • Journal Title: J. Biol. Chem Volume: 286 Pages: 33244-33251
  • Peer Reviewed
• [Journal Article] Identification of nuclear localization signals of Drosophila G9a histone H3 methyltransferase (2011)
  • Author(s): Kato, Y., Ushijima, Y. and Yamaguchi, M.
  • Journal Title: Cell Struct. Funct Volume: 36 Pages: 121-129
  • NAID: 130004137567
  • Peer Reviewed
• [Journal Article] DREF is critical for Drosophila bristle development by regulating endoreplication in shaft cells (2011)
  • Author(s): Kawamori, A. and Yamaguchi, M.
  • Journal Title: Cell Struct. Funct Volume: 36 Pages: 103-119
  • NAID: 130004053879
  • Peer Reviewed
• [Journal Article] A Drosophila model for the screening of bioavailable NADPH oxidase inhibitors and antioxidants (2011)
  • Author(s): Tu Anh, N. T., Nishitani, M., Harada, S., Yamaguchi, M. and Kamei, K.
  • Journal Title: Mol. Cell. Biochem Volume: 352 Pages: 91-98
  • Peer Reviewed
• [Journal Article] NF-Y transcription ally regulates the Drosophila p53 gene (2011)
  • Author(s): Tue, N. T., Yoshioka, Y. and Yamaguchi, M.
  • Journal Title: Gene Volume: 473 Pages: 1-7
  • Peer Reviewed
• [Journal Article] NF-Y transcriptionally regulates the Drosophila p53 gene. (2011)
  • Author(s): Tue, N.T., Yoshioka, Y., Yamaguchi, M.
  • Journal Title: Gene Volume: 473 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Immobilization of protein kinase C into cypovirus polyhedra (2010)
  • Author(s): Ijiri, H., Nakatani, T., Ido, H., Hamada, N., Kotani, E., Mori, H.
  • Journal Title: J. Insect Biotech. Sericol Volume: 79 Pages: 15-20
  • NAID: 10026132179
  • Peer Reviewed
• [Journal Article] Rapid and accurate method for isolation of recombinant baculovirus with an expanded host range (2010)
  • Author(s): Watanabe S, Ohta M, Kokuho T, Mori H, Inumaru S.
  • Journal Title: J. Biosci. Bioeng Volume: 110 Pages: 66-68
  • NAID: 110007681446
  • Peer Reviewed
• [Journal Article] Secreted frizzled related protein4reduces fibrosis scar size and ameliorates cardiac function after ischemic injury (2010)
  • Author(s): Matsushima, K., Suyama, T., Takenaka, C., Nishishita, N., Ikeda, K., Ikada, Y., Sawa, Y., Jakt, L.-M., Mori, H., Kawamata, S
  • Journal Title: Tissue Engineering Part A Volume: 16 Pages: 3329-3341
  • Peer Reviewed
• [Journal Article] Drosophila Syntrophins are involved in locomotion and regulation of synaptic morphology (2010)
  • Author(s): Nagai, R., Hashimoto, R. and Yamaguchi, M.
  • Journal Title: Exp. Cell Res Volume: 316 Pages: 2313-2321
  • Peer Reviewed
• [Journal Article] Regulation of the Drosophila p38b gene by the transcription factor DREF in adult midgut (2010)
  • Author(s): Park, J.-S., Kim, Y.-S., Kim, J.-G., Lee, S.-H., Park, S.-Y., Yamaguchi, M. and Yoo, M.-A.
  • Journal Title: Biochim. Biophys. Acta Volume: 1799 Pages: 510-519
  • Peer Reviewed
• [Journal Article] Role of DREF in transcriptional regulation of the Drosophila p53gene (2010)
  • Author(s): Tue, N. T., Thao, D. T. P. and Yamaguchi, M.
  • Journal Title: Oncogene Volume: 29 Pages: 2060-2069
  • Peer Reviewed
• [Journal Article] Syntrophin-2is required for eye development in Drosophila (2010)
  • Author(s): Nagai, R., Hashimoto, R., Tanaka, Y., Taguchi, O., Sato, M., Matsukage, A. and Yamaguchi, M.
  • Journal Title: Exp. Cell Res Volume: 316 Pages: 272-285
  • Peer Reviewed
• [Journal Article] Role of DREF in transcriptional regulation of the Drosophila p53 gene. (2010)
  • Author(s): Tue, N.T., Thao, D.T.P., Yamaguchi, M.
  • Journal Title: Oncogene Volume: 29 Pages: 2060-2069
  • Peer Reviewed
• [Journal Article] Drosophila Syntrophins are involved in locomotion and regulation of synaptic morphology. (2010)
  • Author(s): Nagai, R., Hashimoto, R., Yamaguchi, M.
  • Journal Title: Exp.Cell Res. Volume: 316 Pages: 2313-2321
  • Peer Reviewed
• [Journal Article] Syntrophin-2 is required for eye development in Drosophila (2010)
  • Author(s): Nagai, R., Hashimoto, R., Tanaka, Y., Taguchi, O., Sato, M., Matsukage, A., Yamaguchi, M.
  • Journal Title: Experimental Cell Research 316 Pages: 272-285
  • Peer Reviewed
• [Journal Article] The atomic structure of baculovirus polyhedra reveals the independent emergence of infectious crystals in DNA and RNA viruses (2009)
  • Author(s): Coulibaly, F., Chiu, E., Gutmann, S., Rajendran, C., Haebel, P. W., Ikeda, K., Mori, H., Ward, V. K., Schulze-Briese, C., Metcalf, P
  • Journal Title: Proc. Nat. Acad. Sci Volume: 106 Pages: 22205-22210
  • Peer Reviewed
• [Journal Article] Structure-based targeting of bioactive proteins into cypovirus polyhedra and application to immobilized-cytokines for mammalian cell culture (2009)
  • Author(s): Ijiri, H., Coulibaly, F., Nishimura, G., Nakai, D., Chiu, E., Takenaka, C., Ikeda, K., Nakazawa, H., Hamada, N., Kotani, E., Metcalf, P., Kawamata, S., Mori, H
  • Journal Title: Biomaterials Volume: 30 Pages: 4297-4308
  • Peer Reviewed
• [Journal Article] Molecular Characterization of a TIA-1 like RNA-binding protein in the cells derived from the fall armyworm (2009)
  • Author(s): Muto, S., Tanabe, T., Matsumoto, E., Mori, H., Kotani, E
  • Journal Title: Spodoptera frugiperda(Lepidoptera : Noctuidae). Biosci. Biotechnol. Biochem Volume: 73 Pages: 648-656
  • Peer Reviewed
• [Journal Article] Functional analysis of the gene of BmTRN-1, an RNA-binding protein homologous to mammalian TIA-1from the silkworm (2009)
  • Author(s): Muto, S., Matsumoto, E., Tanabe, T., Mori, H., Kotani, E.
  • Journal Title: Bombyx mori ; a study of it's over expression and sub-cellular distribution during the baculovirus infection process. J. Insect Biotech. Sericol Volume: 78 Pages: 39-51
  • Peer Reviewed
• [Journal Article] Pret porter, a Drosophila protein serving as a ligand for Draper in the phagocytosis of apoptotic cells (2009)
  • Author(s): Kuraishi, T., Nakagawa, Y., Nagaosa, K., Hashimoto, Y., Ishimoto, T., Moki, T., Fujita, Y., Nakayama, H., Dohmae, N., Shiratsuchi, A., Yamamoto, N., Ueda, K., Yamaguchi, M., Awasaki, T. and Nakanishi, Y.
  • Journal Title: EMBO J Volume: 28 Pages: 3868-3878
  • Peer Reviewed
• [Journal Article] A novel Drosophila Girdin-like protein is involved in Akt pathway control of cell size (2009)
  • Author(s): Puseenam A., Yoshioka Y., Nagai, R., Hashimoto R., Suyari, O., Itoh, M., Enomoto, A., Takahashi, M. and Yamaguchi, M.
  • Journal Title: Exp. Cell Res Volume: 315 Pages: 3370-3380
  • Peer Reviewed
• [Journal Article] Identification of the Drosophila Mes4 gene as a novel target of the transcription factor DREF (2009)
  • Author(s): Suyari, O., Ida, H., Yoshioka, Y., Kato, Y., Hashimoto, R. and Yamaguchi, M.
  • Journal Title: Exp. Cell Res Volume: 315 Pages: 1403-1414
  • Peer Reviewed
• [Journal Article] Genetic screening for modifiers of the DREF pathway in Drosophila melanogaster : Identification and characterization of HP6 as a novel target of DREF (2009)
  • Author(s): Ida, H., Suzusho, N., Suyari, O., Yoshida, H., Ohno, K., Hirose, F., Itoh, M. and Yamaguchi, M.
  • Journal Title: Nucleic Acids Res Volume: 37 Pages: 1423-1437
  • Peer Reviewed
• [Presentation] キイロショウジョウバエDOCK family CG31048遺伝子のR7光受容細胞分化への関与 (2011)
  • Author(s): 江口晃一、吉岡泰秀、吉田英樹、宮田清司、日合弘、山口政光
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
  • Year and Date: 2011-12-16
• [Presentation] Involvement of dMLF in JNK signaling pathway (2011)
  • Author(s): Yanai, H., Nakao, Y., Yoshioka, Y., Yoshida, H. and Yamaguchi, M.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
  • Year and Date: 2011-12-16
• [Presentation] ショウジョウバエ骨髄性白血病因子dMLFのJNKシグナル伝達経路への関与 (2010)
  • Author(s): 梁井啓史、中尾祐美子、吉岡泰秀、吉田英樹、山口政光
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸市・神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] 遺伝性神経変性疾患ALS(筋萎縮性側索硬化症Amyotrophic Lateral Sclerosis)モデルショウジョウバエを用いた病態解析 (2010)
  • Author(s): 笹山博司、島村真依、徳田隆彦、山口政光、中川正法
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸市・神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] NF-Y transcriptionally regulates the Drosophila p53 gene. (2010)
  • Author(s): Tue Nguyen Trong, Yasuhide Yoshioka, Masamitsu Yamaguchi
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸市・神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] Genetic screen for Drosophila myeloid leukemia factor(dMLF) interactants and analysis of dMLF in JNK pathway (2009)
  • Author(s): Yanai, H., Nakao, Y., Yoshioka, Y. and Yamaguchi, M.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] DREF, a novel transcriptional regulatory factor of the Drosophila p53gene (2009)
  • Author(s): Tue, N. T., Thao, D. T. P. and Yamaguchi, M.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
  • Year and Date: 2009-12-09
• [Presentation] In vivo function of Drosophila Syntrophin, a component of the DGC (2009)
  • Author(s): Nagai, R., Hashimoto, R., Tanaka, Y., Sato, M., Matsukage, A. and Yamaguchi, M.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
  • Year and Date: 2009-12-09
• [Presentation] DGC構成因子の一つであるショウジョウバエシントロフィンの生体内機能 (2009)
  • Author(s): 永井里佳, 橋本麗奈, 田中裕子, 佐藤真美子, 松影昭夫, 山口政光
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: パシフィコ横浜 (神奈川)
  • Year and Date: 2009-12-09
• [Presentation] Cypovirus polyhedra : structure and application (2009)
  • Author(s): Mori, H.
  • Organizer: International Symposium on Recent Bio-Research Works in KIT of Japan and PNU of Korea
  • Place of Presentation: 韓国、釜山市・釜山大学
  • Year and Date: 2009-09-11
• [Remarks] 京都工芸繊維大学・応用生物学部門・染色体工学研究室ホームページ
  • URL: http://www.bio.kit.ac.jp/chromosome/PUBLICATION.html
• [Remarks]
  • URL: http://www.bio.kit.ac.jp/chromosome/INDEX.html