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Development of novel drug screening process by using Drosophila disease models and silk worm Cypovirus function

Research Project

Project/Area Number 21580067
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied entomology
Research InstitutionKyoto Institute of Technology

Principal Investigator

YAMAGUCHI Masamitsu  京都工芸繊維大学, 工芸科学研究科, 教授 (00182460)

Co-Investigator(Kenkyū-buntansha) MORI Hajime  京都工芸繊維大学, 工芸科学研究科, 教授 (80201812)
HASHIMOTO Reina  京都工芸繊維大学, ベンチャーラボラトリー, 研究員 (20467492)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords昆虫 / 遺伝子 / 応用動物 / 生体分子 / 遺伝学
Research Abstract

β-sarcoglycan contains EGF-like amino acid sequences in its extracellular domain. In vitro analyses demonstrated thatβ-sarcoglycan can interacts with EGFR via this extracellular domain. Double mutant for Syntrophin1and2has been established. Analyses with this double mutant clarified in vivo roles of these two genes. Recombinantβ-sarcoglycan tagged with Cypovirus VP3has been successfully expressed in cultured Drosophila cells.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (52 results)

All 2012 2011 2010 2009 Other

All Journal Article (40 results) (of which Peer Reviewed: 40 results) Presentation (10 results) Remarks (2 results)

  • [Journal Article] Fibroblast growth factor-2(Fgf2) is an important factor that maintains cellular immaturity and contributes to aggressiveness of osteosarcoma2012

    • Author(s)
      Shimizu, T., Ishikawa, T., Iwai, S., Ueki, A., Sugihara, E., Onishi, N., Kuninaka, S., Miyamoto, T., Toyama, Y., Ijiri, H., Mori, H., Matsuzaki, Y., Yaguchi, T., Nishio, H., Kawakami, Y., Ikeda, Y., Saya, H.
    • Journal Title

      Mol. Cancer Res

      Volume: 10 Pages: 454-468

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] XNP/dATRX interacts with DREF in the chromatin to regulate gene expression2012

    • Author(s)
      Valadez-Graham, V., Yoshioka, Y., Velazquez, O., Kawamori, A. Vazquez, M., Neumann, A., Yamaguchi, M. and Zurita, M.
    • Journal Title

      Nucleic Acids Res

      Volume: 40(4) Pages: 1460-1474

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The warts gene as a novel target of the Drosophila DRE/DREF transcription pathway2012

    • Author(s)
      Fujiwara, S., Ida, H., Yoshioka, Y., Yoshida, H. and Yamaguchi, M.
    • Journal Title

      Am J. Cancer Res

      Volume: 2(1) Pages: 36-44

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transcription factor NF-Y is involved in differentiation of R7 photoreceptor cell in Drosophila2012

    • Author(s)
      Yoshioka, Y., Ly, L. L. and Yamaguchi, M.
    • Journal Title

      Biology Open

      Volume: 1 Issue: 1 Pages: 19-29

    • DOI

      10.1242/bio.2011013

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The warts gene as a novel target of the Drosophila DRE/DREF transcription pathway2012

    • Author(s)
      Fujiwara, S., Ida, H., Yoshioka, Y., Yoshida, H., Yamaguchi, M
    • Journal Title

      Am J.Cancer Res.

      Volume: 2 Pages: 36-44

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] XNP/dATRX interacts with DREF in the chromatin to regulate gene expression2012

    • Author(s)
      Valadez-Graham, V., Yoshioka, Y., Velazquez, O., Kawamori, A., Vazquez, M., Neumann, A., Yamaguchi, M., Zurita, M.
    • Journal Title

      Nucleic Acids Res.

      Volume: 40 Pages: 1460-1474

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of αB-crystallin on protein aggregation in Drosophila2012

    • Author(s)
      Tue, N.T., Shimaji, K., Tanaka, N., Yamaguchi, M.
    • Journal Title

      J.Biomed.Biotech.

      Volume: 2012 Pages: 1-7

    • DOI

      10.1155/2012/252049

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential requirement for the N-terminal catalytic domain of the DNA polymerase ε p255 subunit in the mitotic cell cycle and the endocycle2012

    • Author(s)
      Suyari, O., Kawai, M., Ida, H., Yoshida, H., Sakaguchi, K., Yamaguchi, M.
    • Journal Title

      Gene

      Volume: 495 Pages: 104-114

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Drosophila DREF acting via the JNK pathway is required for thorax development2012

    • Author(s)
      Yoshioka, Y., Tue, N.T., Fujiwara, S., Matsuda, R., Valadez-Gtaham, V., Zurita, M., Yamaguchi, M.
    • Journal Title

      Genesis

      Volume: (印刷中) Issue: 8 Pages: 599-611

    • DOI

      10.1002/dvg.22017

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Roles of histone H3K9 methyltransferases during Drosophila spermatogenesis2012

    • Author(s)
      Ushijima Y., Inoue, Y.H., Konishi, T., Kitazawa, D., Yoshida, H., Shimaji, K., Kimura, H., Yamaguchi, M.
    • Journal Title

      Chromosome Res.

      Volume: 20 Pages: 319-331

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Polycomb repressive complex 2-dependent and -independent functions of Jarid2 in transcriptional regulation in Drosophila2012

    • Author(s)
      Herz, H.-M., Mohan, M., Garrett, A.-S., Miller, C., Casto, D., Zhang, Y., Seidel, C., Haug, J.S., Florens, L., Washburn, M.P., Yamaguchi, M., Shiekhattar, R., Shilatifard, A.
    • Journal Title

      Mol.Cell.Biol.

      Volume: 32 Pages: 319-331

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] MLF is a conserved regulator of RUNX transcription factor activity involved in hematopoiesis2012

    • Author(s)
      Bras, S., Martin-Lanneree, S., Gobert, V., Auge, B., Breig, O., Sanial, M., Yamaguchi, M., Haenlin, M., Plessis, A., Waltzer, L
    • Journal Title

      Proc.Natl.Acad.Sci.USA

      Volume: 109 Pages: 4986-4991

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Overexpression of ubiquitin carboxyl terminal hydrolase impairs multipie pathways during eye development in Drosophila melanogaster2012

    • Author(s)
      Thao, D.T.P, Thuy An, P.N., Yamaguchi, M., Thuoc, T.L
    • Journal Title

      Cell and Tissue Research

      Volume: (印刷中)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The use of leukemia inhibitory factor immobilized on virus-derived polyhedra to support the proliferation of mouse embryonic and induced pluripotent stem cells2011

    • Author(s)
      Nishishita, N., Ijiri, H., Takenaka, C., Kobayashi, K., Goto, K., Kotani, E., Itoh, T., Mori, H., Kawamata, S.
    • Journal Title

      Biomaterials

      Volume: 32 Pages: 3555-3563

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of a novel C-type lectin cDNA, CLEM20cDNA, specifically expressed in mouthparts of the flesh fly Sarcopahaga peregrina2011

    • Author(s)
      Yamamoto-Kihara, M., Kotani, E., Mori, H.
    • Journal Title

      J. Insect Biotech. Sericol

      Volume: 80 Pages: 31-39

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of intestinal stem cell proliferation by human methyl-CpG-binding protein-2in Drosophila2011

    • Author(s)
      Lee, S.-H., Kim, I-J., Kim, J.-G., Kim, Y.-S., Yamaguchi, M. and Yoo, M.-A.
    • Journal Title

      Cell Struct. Funct

      Volume: 36(2) Pages: 197-208

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Essential role of Duox in the stabilization of Drosophila wing2011

    • Author(s)
      Tu Anh, N. T., Nishitani, M., Harada, S., Yamaguchi, M. and Kamei, K.
    • Journal Title

      J. Biol. Chem

      Volume: 286 Pages: 33244-33251

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of nuclear localization signals of Drosophila G9a histone H3 methyltransferase2011

    • Author(s)
      Kato, Y., Ushijima, Y. and Yamaguchi, M.
    • Journal Title

      Cell Struct. Funct

      Volume: 36 Pages: 121-129

    • NAID

      130004137567

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] DREF is critical for Drosophila bristle development by regulating endoreplication in shaft cells2011

    • Author(s)
      Kawamori, A. and Yamaguchi, M.
    • Journal Title

      Cell Struct. Funct

      Volume: 36 Pages: 103-119

    • NAID

      130004053879

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Drosophila model for the screening of bioavailable NADPH oxidase inhibitors and antioxidants2011

    • Author(s)
      Tu Anh, N. T., Nishitani, M., Harada, S., Yamaguchi, M. and Kamei, K.
    • Journal Title

      Mol. Cell. Biochem

      Volume: 352 Pages: 91-98

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] NF-Y transcription ally regulates the Drosophila p53 gene2011

    • Author(s)
      Tue, N. T., Yoshioka, Y. and Yamaguchi, M.
    • Journal Title

      Gene

      Volume: 473 Pages: 1-7

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] NF-Y transcriptionally regulates the Drosophila p53 gene.2011

    • Author(s)
      Tue, N.T., Yoshioka, Y., Yamaguchi, M.
    • Journal Title

      Gene

      Volume: 473 Pages: 1-7

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immobilization of protein kinase C into cypovirus polyhedra2010

    • Author(s)
      Ijiri, H., Nakatani, T., Ido, H., Hamada, N., Kotani, E., Mori, H.
    • Journal Title

      J. Insect Biotech. Sericol

      Volume: 79 Pages: 15-20

    • NAID

      10026132179

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rapid and accurate method for isolation of recombinant baculovirus with an expanded host range2010

    • Author(s)
      Watanabe S, Ohta M, Kokuho T, Mori H, Inumaru S.
    • Journal Title

      J. Biosci. Bioeng

      Volume: 110 Pages: 66-68

    • NAID

      110007681446

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Secreted frizzled related protein4reduces fibrosis scar size and ameliorates cardiac function after ischemic injury2010

    • Author(s)
      Matsushima, K., Suyama, T., Takenaka, C., Nishishita, N., Ikeda, K., Ikada, Y., Sawa, Y., Jakt, L.-M., Mori, H., Kawamata, S
    • Journal Title

      Tissue Engineering Part A

      Volume: 16 Pages: 3329-3341

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Drosophila Syntrophins are involved in locomotion and regulation of synaptic morphology2010

    • Author(s)
      Nagai, R., Hashimoto, R. and Yamaguchi, M.
    • Journal Title

      Exp. Cell Res

      Volume: 316 Pages: 2313-2321

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of the Drosophila p38b gene by the transcription factor DREF in adult midgut2010

    • Author(s)
      Park, J.-S., Kim, Y.-S., Kim, J.-G., Lee, S.-H., Park, S.-Y., Yamaguchi, M. and Yoo, M.-A.
    • Journal Title

      Biochim. Biophys. Acta

      Volume: 1799 Pages: 510-519

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of DREF in transcriptional regulation of the Drosophila p53gene2010

    • Author(s)
      Tue, N. T., Thao, D. T. P. and Yamaguchi, M.
    • Journal Title

      Oncogene

      Volume: 29 Pages: 2060-2069

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Syntrophin-2is required for eye development in Drosophila2010

    • Author(s)
      Nagai, R., Hashimoto, R., Tanaka, Y., Taguchi, O., Sato, M., Matsukage, A. and Yamaguchi, M.
    • Journal Title

      Exp. Cell Res

      Volume: 316 Pages: 272-285

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of DREF in transcriptional regulation of the Drosophila p53 gene.2010

    • Author(s)
      Tue, N.T., Thao, D.T.P., Yamaguchi, M.
    • Journal Title

      Oncogene

      Volume: 29 Pages: 2060-2069

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Drosophila Syntrophins are involved in locomotion and regulation of synaptic morphology.2010

    • Author(s)
      Nagai, R., Hashimoto, R., Yamaguchi, M.
    • Journal Title

      Exp.Cell Res.

      Volume: 316 Pages: 2313-2321

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Syntrophin-2 is required for eye development in Drosophila2010

    • Author(s)
      Nagai, R., Hashimoto, R., Tanaka, Y., Taguchi, O., Sato, M., Matsukage, A., Yamaguchi, M.
    • Journal Title

      Experimental Cell Research 316

      Pages: 272-285

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The atomic structure of baculovirus polyhedra reveals the independent emergence of infectious crystals in DNA and RNA viruses2009

    • Author(s)
      Coulibaly, F., Chiu, E., Gutmann, S., Rajendran, C., Haebel, P. W., Ikeda, K., Mori, H., Ward, V. K., Schulze-Briese, C., Metcalf, P
    • Journal Title

      Proc. Nat. Acad. Sci

      Volume: 106 Pages: 22205-22210

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structure-based targeting of bioactive proteins into cypovirus polyhedra and application to immobilized-cytokines for mammalian cell culture2009

    • Author(s)
      Ijiri, H., Coulibaly, F., Nishimura, G., Nakai, D., Chiu, E., Takenaka, C., Ikeda, K., Nakazawa, H., Hamada, N., Kotani, E., Metcalf, P., Kawamata, S., Mori, H
    • Journal Title

      Biomaterials

      Volume: 30 Pages: 4297-4308

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Characterization of a TIA-1 like RNA-binding protein in the cells derived from the fall armyworm2009

    • Author(s)
      Muto, S., Tanabe, T., Matsumoto, E., Mori, H., Kotani, E
    • Journal Title

      Spodoptera frugiperda(Lepidoptera : Noctuidae). Biosci. Biotechnol. Biochem

      Volume: 73 Pages: 648-656

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional analysis of the gene of BmTRN-1, an RNA-binding protein homologous to mammalian TIA-1from the silkworm2009

    • Author(s)
      Muto, S., Matsumoto, E., Tanabe, T., Mori, H., Kotani, E.
    • Journal Title

      Bombyx mori ; a study of it's over expression and sub-cellular distribution during the baculovirus infection process. J. Insect Biotech. Sericol

      Volume: 78 Pages: 39-51

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pret porter, a Drosophila protein serving as a ligand for Draper in the phagocytosis of apoptotic cells2009

    • Author(s)
      Kuraishi, T., Nakagawa, Y., Nagaosa, K., Hashimoto, Y., Ishimoto, T., Moki, T., Fujita, Y., Nakayama, H., Dohmae, N., Shiratsuchi, A., Yamamoto, N., Ueda, K., Yamaguchi, M., Awasaki, T. and Nakanishi, Y.
    • Journal Title

      EMBO J

      Volume: 28 Pages: 3868-3878

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel Drosophila Girdin-like protein is involved in Akt pathway control of cell size2009

    • Author(s)
      Puseenam A., Yoshioka Y., Nagai, R., Hashimoto R., Suyari, O., Itoh, M., Enomoto, A., Takahashi, M. and Yamaguchi, M.
    • Journal Title

      Exp. Cell Res

      Volume: 315 Pages: 3370-3380

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the Drosophila Mes4 gene as a novel target of the transcription factor DREF2009

    • Author(s)
      Suyari, O., Ida, H., Yoshioka, Y., Kato, Y., Hashimoto, R. and Yamaguchi, M.
    • Journal Title

      Exp. Cell Res

      Volume: 315 Pages: 1403-1414

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genetic screening for modifiers of the DREF pathway in Drosophila melanogaster : Identification and characterization of HP6 as a novel target of DREF2009

    • Author(s)
      Ida, H., Suzusho, N., Suyari, O., Yoshida, H., Ohno, K., Hirose, F., Itoh, M. and Yamaguchi, M.
    • Journal Title

      Nucleic Acids Res

      Volume: 37 Pages: 1423-1437

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Presentation] キイロショウジョウバエDOCK family CG31048遺伝子のR7光受容細胞分化への関与2011

    • Author(s)
      江口晃一、吉岡泰秀、吉田英樹、宮田清司、日合弘、山口政光
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜市・パシフィコ横浜
    • Year and Date
      2011-12-16
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Involvement of dMLF in JNK signaling pathway2011

    • Author(s)
      Yanai, H., Nakao, Y., Yoshioka, Y., Yoshida, H. and Yamaguchi, M.
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜市・パシフィコ横浜
    • Year and Date
      2011-12-16
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] ショウジョウバエ骨髄性白血病因子dMLFのJNKシグナル伝達経路への関与2010

    • Author(s)
      梁井啓史、中尾祐美子、吉岡泰秀、吉田英樹、山口政光
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸市・神戸ポートアイランド
    • Year and Date
      2010-12-10
    • Related Report
      2011 Final Research Report
  • [Presentation] 遺伝性神経変性疾患ALS(筋萎縮性側索硬化症Amyotrophic Lateral Sclerosis)モデルショウジョウバエを用いた病態解析2010

    • Author(s)
      笹山博司、島村真依、徳田隆彦、山口政光、中川正法
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸市・神戸ポートアイランド
    • Year and Date
      2010-12-08
    • Related Report
      2011 Final Research Report
  • [Presentation] NF-Y transcriptionally regulates the Drosophila p53 gene.2010

    • Author(s)
      Tue Nguyen Trong, Yasuhide Yoshioka, Masamitsu Yamaguchi
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸市・神戸ポートアイランド
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Genetic screen for Drosophila myeloid leukemia factor(dMLF) interactants and analysis of dMLF in JNK pathway2009

    • Author(s)
      Yanai, H., Nakao, Y., Yoshioka, Y. and Yamaguchi, M.
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜市・パシフィコ横浜
    • Year and Date
      2009-12-10
    • Related Report
      2011 Final Research Report
  • [Presentation] DREF, a novel transcriptional regulatory factor of the Drosophila p53gene2009

    • Author(s)
      Tue, N. T., Thao, D. T. P. and Yamaguchi, M.
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜市・パシフィコ横浜
    • Year and Date
      2009-12-09
    • Related Report
      2011 Final Research Report
  • [Presentation] In vivo function of Drosophila Syntrophin, a component of the DGC2009

    • Author(s)
      Nagai, R., Hashimoto, R., Tanaka, Y., Sato, M., Matsukage, A. and Yamaguchi, M.
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜市・パシフィコ横浜
    • Year and Date
      2009-12-09
    • Related Report
      2011 Final Research Report
  • [Presentation] DGC構成因子の一つであるショウジョウバエシントロフィンの生体内機能2009

    • Author(s)
      永井里佳, 橋本麗奈, 田中裕子, 佐藤真美子, 松影昭夫, 山口政光
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜 (神奈川)
    • Year and Date
      2009-12-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] Cypovirus polyhedra : structure and application2009

    • Author(s)
      Mori, H.
    • Organizer
      International Symposium on Recent Bio-Research Works in KIT of Japan and PNU of Korea
    • Place of Presentation
      韓国、釜山市・釜山大学
    • Year and Date
      2009-09-11
    • Related Report
      2011 Final Research Report
  • [Remarks] 京都工芸繊維大学・応用生物学部門・染色体工学研究室ホームページ

    • URL

      http://www.bio.kit.ac.jp/chromosome/PUBLICATION.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.bio.kit.ac.jp/chromosome/INDEX.html

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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